Nurullahoglu-Atalik, K. E.Oz, M.Shafiyi, A.2024-02-232024-02-2320150006-92481336-0345https://doi.org/10.4149/BLL_2015_095https://hdl.handle.net/20.500.12452/16315OBJECTIVE: The effects of Rho-kinase inhibitors on vasodilatation induced by 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor rosuvastatin (10(-9)-10(-4)M) on 5-HT-precontracted calf cardiac vein and the role of endothelium in these effects were analyzed. MATERIAL AND METHODS: Cardiac vein ring preparations were suspended in organ baths containing 25 ml of Krebs Henseleit solution, maintained at 37 degrees C and continuously gassed with 95 % O-2-5 % CO2. At the end of the resting period, the cardiac vein preparations were contracted with 10(-6) M 5-HT. After the contraction had reached a steady state, rosuvastatin was added to the organ bath cumulatively (10(-9)-10(-4)M). RESULTS: Rosuvastatin relaxed the cardiac vein rings in general while the degree of relaxation was greater in those with endothelium and lower in those without it. HA1077 [1-(5-isoquinolinesulfonyl)-homopiperazine] (Fasudil, 10(-6) M) and Y-27632 [(+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclohexane carboxamide dihydrochloride] (10(-6) M) incubation increased the rosuvastatin-induced relaxation only in the presence of endothelium. CONCLUSIONS: The results demonstrate for the first time that in calf cardiac vein, rosuvastatin induced endothelium-dependent relaxations while Rho-kinase inhibition increased these relaxations in the presence of endothelium layer (Fig. 3, Ref. 44). Text in PDF www.elis.sk.eninfo:eu-repo/semantics/openAccessCalf Cardiac VeinEndothelium-Dependent RelaxationRho-KinaseRosuvastatinArticle1168494498263500902-s2.0-84962269374Q3WOS:000361083000008Q410.4149/BLL_2015_095