Aydin, ZiyaKeskinates, MukaddesYilmaz, BaharDurmaz, MustafaBayrakci, Mevlut2024-02-232024-02-2320220003-26971096-0309https://doi.org/10.1016/j.ab.2022.114798https://hdl.handle.net/20.500.12452/11532Cyanide ion (CN-) is widely used in many industrial processes; however, it causes several diseases in humans. Therefore, rapid and accurate detection of CN- is very important and urgent. In this study, a CN- sensor (MH-2) which was capable of detecting CN- ions in living cell was developed. MH-2 gives a rapid color change, absorbance and fluorescence response to CN- in the presence of the anions tested in the working system. The binding ratio between the sensor and CN- was demonstrated by some spectrophotometric methods and the sensing mechanism was investigated by NMR titration experiments, suggesting that MH-2 gives response to CN- via the nucleophilic addition reaction. The fluorescence detection limit and the absorbance detection limit were calculated as 0.056 mu M and 0.11 mu M, respectively. Both of these detection limits are below the tolerable limit recommended by WHO for CN- in the drinking water (1.9 mu M). MH-2 was also applied to living cells for bio-imaging and the results showed that the sensor penetrates the cells and can detect cyanide ions in living cells.eninfo:eu-repo/semantics/closedAccessColorimetric SensorFluorescent SensorCyanide SensingBioimagingArticle654357795722-s2.0-85133430138Q3WOS:000841161600003Q210.1016/j.ab.2022.114798