Sahin, MerveArioglu-Tuncil, SedaUnver, AhmetDeemer, DaneLindemann, Stephen R.Tuncil, Yunus E.2024-02-232024-02-2320230021-85611520-5118https://doi.org/10.1021/acs.jafc.3c01415https://hdl.handle.net/20.500.12452/12720This study aimed to evaluate and compare the effectsof dietaryfibers (DFs) of commercially important tree nuts (almond, cashew,hazelnut, pistachio, and walnut) on gut microbiota in vitro. Microbial compositions and short-chain fatty acids were determinedusing 16S rRNA sequencing and gas chromatography (GC), respectively.Neutral and acidic monosaccharides were analyzed using GC/MS and spectrophotometry,respectively. Our results revealed that cashew fibers exhibit higherbutyrate formation compared to others. Accordingly, cashew fiber promotedbutyric acid-producing bacteria-related operational taxonomic units(OTUs; Butyricimonas and Collinsella) at higher relative abundances. The higher butyrogenic capacityof cashew fiber is mainly attributed to its higher soluble/total DFratio and remarkably distinct monosaccharide composition. Additionally,nut fibers stimulated family Lachnospiraceae- and Ruminococcaceae-related OTUs. These findings show that althoughthe degree of promotion is nut type-dependent, nut fibers are generallycapable of promoting beneficial microbes in the colon, further suggestingthat DFs of tree nuts are contributing factors to their health-promotingeffects.eninfo:eu-repo/semantics/closedAccessAlmondHazelnutPistachioWalnutColonMicrobiome16s RrnaArticle712597629771373344682-s2.0-85164210420Q1WOS:00101151930000110.1021/acs.jafc.3c01415