Simsek, AyselPehlivanoglu, SurayAcar, Cigdem Aydin2024-02-232024-02-2320212190-572X2190-5738https://doi.org/10.1007/s13205-021-02923-4https://hdl.handle.net/20.500.12452/11424In this study, we aimed at the green synthesis of silver nanoparticles (AgNPs) using Lavandula angustifolia extract and the investigation of the anti-proliferative and apoptotic inducing effects of these nanoparticles in the U87MG glioblastoma cancer cell line. Green synthesized silver nanoparticles were characterized by various analytical techniques such as UV-Visible Spectrophotometer (UV-Vis), scanning electron microscopy (SEM) and Energy Dispersive X-ray (EDX). UV-Vis spectroscopy displayed a specific silver plasmon peak at 430 nm. U87MG cells were treated at increased concentrations with Lavandula angustifolia-AgNPs (La-AgNPs) (0-20 mu g/mL) for 72 h and the anti-proliferative effects of green synthesized silver nanoparticles on U87MG cells were evaluated by MTT assay. The La- AgNPs induced a statistically significant dose-dependent decrease in proliferation and increased cytotoxicity in U87MG cells. The IC50 value is 7.536 mu g/mL. Furthermore, the expression of apoptosis proteins caspase-3, caspase-8 and caspase-9 was analyzed using ELISA and caspase-3 and p53 using western blotting. The results suggest that La-AgNPs induce cell death in U87MG cells through the p53 mediated intrinsic apoptotic pathway. Together, the present findings suggest that La-AgNPs could be considered as a potential option for the treatment of glioblastoma.eninfo:eu-repo/semantics/openAccessSilver NanoparticlesAgnpsGlioblastomaU87mgArticle118343678662-s2.0-85110736025Q2WOS:000691836900001Q310.1007/s13205-021-02923-4