Çakmak, DeryaKaraoğlu, CumaAasım, MuhammadSancak, CengizÖzcan, Sebahatin2020-01-182020-01-182016Aasim, M., Çakmak, D., Karaoğlu, C., Sancak, C., Özcan, S. (2016). Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica. Turkish Journal of Biology, 40, 4, 878-888.1300-01521303-6092https://app.trdizin.gov.tr/makale/TWpFME56UXhNUT09/advancement-in-protocol-for-in-vitro-seed-germination-regeneration-bulblet-maturation-and-acclimatization-of-fritillaria-persicahttps://hdl.handle.net/20.500.12452/1421WOS:000378689600017t: The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening, and acclimatization of Persian lily (Fritillaria persica). Seed germination rate was recorded as 86.7% and 96.7% after 2 and 3 months of cold treatment at 4 °C, respectively. Bulblet explants taken from a germination experiment were cultured on Murashige and Skoog (MS) medium supplemented with 0.5–2.0 mg/L thidiazuron (TDZ). Maximum bulblet regeneration frequency and bulblets per explants were achieved on a medium containing 2.0 mg/L TDZ. Leaf disc and leaf scale explants isolated from germinated seedlings were also cultured on MS medium containing 2–6 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) with a constant concentration of 0.2 mg/L kinetin. Both explants yielded the highest bulblet regeneration on MS medium containing 2 mg/L 2,4-D and 0.2 mg/L kinetin. A combination of 1.5 g/L agar and 1.6 g/L GELRITE resulted in a maximum bulblet diameter with a higher number of roots. The addition of 10 g/L NaCl to the culture medium resulted in the best root and leaf formation and secondary bulblet regeneration. Exposure of regenerated bulblets to 10 g/L NaCl at 4 °C for 2 months on a medium with 20 g/L sucrose was also found to be beneficial for acclimatization.eninfo:eu-repo/semantics/openAccessFritillaria persicaMicropropagationSecondary bulbletsBulblet sizeHardeningArticle404878888Q2WOS:000378689600017Q310.3906/biy-1510-18