Cetinkaya, Yagmur NurBulut, OnurOktem, Huseyin AvniYilmaz, M. Deniz2024-02-232024-02-2320231386-14251873-3557https://doi.org/10.1016/j.saa.2023.123222https://hdl.handle.net/20.500.12452/12551Herein, we report fluorescein-labelled silica nanoparticles (FSNP) which serve as fluorescent nano-chemosensors for sequential detection of Pb2+ (which is a toxic heavy metal) and dipicolinic acid (DPA) (which is a distinctive indicator biomarker of bacterial spores) with high sensitivity and selectivity. The fluorescence of FSNP is quenched because of the complex formation between Pb2+ ions and surface amide groups, however, the fluorescence is recovered in contact with DPA, resulting from the association of DPA with surface bound Pb2+ ions. FSNP-Pb2+ complexes show high sensitivity towards DPA with a low detection limit of 850 nM which is approximately seventy times lower than the infectious dosage of bacterial spores (60 & mu;M). Lateral flow test platform was further developed to show the applicability and practicability of our system.eninfo:eu-repo/semantics/closedAccessBacterial SporesDipicolinic AcidPb2+Silica NanoparticlesFluorescenceLateral FlowArticle303375428712-s2.0-85169883742Q2WOS:00107130960000110.1016/j.saa.2023.123222