The role of oxidative stress in ?-amanitin-induced hepatotoxicity in an experimental mouse model
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CitationDündar, Z. D., Ergin, M., Kilinç, I., Çolak, T., Oltulu, P., Cander, B. (2017). The role of oxidative stress in α-amanitin-induced hepatotoxicityin an experimental mouse model. Turkish Journal of Medical Sciences, 47, 1, 318-325.
Background/aim: This study aimed to evaluate oxidative stress markers of liver tissue in a mouse &#945;-amanitin poisoning model with three different toxin levels. Materials and methods: The mice were randomly divided into Group 1 (control), Group 2 (0.2 mg/kg), Group 3 (0.6 mg/kg), and Group 4 (1.0 mg/kg). The toxin was injected intraperitoneally and 48 h of follow-up was performed before sacrifice. Results: Median superoxide dismutase activities of liver tissue in Groups 3 and 4 were significantly higher than in Group 1 (for both, P 0.001). The catalase activity in Group 2 was significantly higher, but in Groups 3 and 4 it was significantly lower than in Group 1 (for all, P 0.001). The glutathione peroxidase activities in Groups 2, 3, and 4 were significantly higher than in Group 1 (P 0.006, P 0.001, and P 0.001, respectively). The malondialdehyde levels of Groups 3 and 4 were significantly higher than Group 1 (P 0.015 and P 0.003, respectively). The catalase activity had significant correlations with total antioxidant status and total oxidant status levels (r 0.935 and r 0.789, respectively; for both, P > 0.001). Conclusion: Our findings support a significant role for increased oxidative stress in &#945;-amanitin-induced hepatotoxicity.
SourceTurkish Journal of Medical Sciences