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Öğe A case with buccal squamous cell carcinoma that developed pemphigus vulgaris induced by radiotherapy(Wiley, 2020) Daye, Munise; Findik, Siddika; Durmaz, Koray[Abstract Not Availabe]Öğe Comparison of primary repair and repair with polyglycolic acid coated tube in recurrent laryngeal nerve cuts (an experimental study)(Excerpta Medica Inc-Elsevier Science Inc, 2020) Senturk, Mustafa; Cakir, Murat; Tekin, Ahmet; Kucukkartallar, Tevfik; Yildirim, Mehmet Aykut; Alkan, Selman; Findik, SiddikaBackground: Recurrent laryngeal nerve (RLN) injury is an important complication of thyroid surgery. In our study, we aimed to compare the effects of primary repair and polyglycolic acid (PGA) coated tube repair on nerve function and regeneration in RLN cuts in rats. Methods: Twenty seven rats were used for the study. Group-1 with only nerve cuts, group-2 with primary repair, and group-3 with conduit repair. The study was planned over two-stage surgery. In the first step, nerve defect formation and nerve repair were performed in the same session. In the second step, samples were taken from the subjects for histopathological. Results: Vocal cord mobility was proportionally higher in group-3 than other groups. However, there was no statistically significant difference between the groups (p < 0.239). The mean number of highest axons were detected the group-3. Conclusions: We think that PGA coated conduit can provide a more accurate orientation of nerve fibers by creating an isolated environment when compared to the primary repair. And this may be the cause of functional improvement in the nerve. (C) 2019 Elsevier Inc. All rights reserved.Öğe Comparison of the efficacy of growth factor collagen and antibiotic collagen on colon anastomosis in experimental animals with peritonitis(Springer India, 2021) Yildirim, Mehmet Aykut; Cakir, Murat; Findik, Siddika; Kisi, Omer; Senturk, MustafaIntroduction In spite of advances in surgical techniques, the significance of anastomosis leak continues in colorectal surgery. There is no ideal method in spite of all studies and technical advances in this field. Our aim of this study was to use fibroblast growth factor collagen (FGF-C) and antibiotic collagen (AB-C) to increase the rate of anastomosis healing in experimental animals with peritonitis. Methods This animal experimental study received ethics committee approval. The animals were divided into three groups of seven animals each; the first group was control, the second group was the fibroblast growth factor collagen group, and the third group was the antibiotic collagen group. Under anesthesia, more than 50% of the colonic lumen was opened 4-5 cm distal to the ileocecal junction to create a defect. Twenty-four hours later, primary anastomosis was performed. The second group had the anastomosis line covered with a cover containing FGF-C. The third group had the anastomosis line covered by material containing AB-C. The experiment was concluded on the postoperative 7th day, and the anastomosis burst pressure, tissue hydroxyproline level, and histopathological assessment were performed. Results Though the burst pressure was higher in the experimental groups, it was not statistically significant. In the second and third groups, vascular proliferation and fibroblastic activity appeared to be better than in the control group. Hydroxyproline values were statistically significant in the experimental groups compared to the control group. Conclusion FGF-C and AB-C may have potential utility in anastomosis healing, especially in those susceptible to infection due to anastomosis leak.Öğe Cutaneous Metastases of the Synchronous Primary Endometrial and Bilateral Ovarian Cancer: An Infrequent Presentation and Literature Review(Hindawi Ltd, 2016) Kanyilmaz, Gul; Aktan, Meryem; Koc, Mehmet; Findik, SiddikaThere are limited data about the cutaneous metastases of gynecological malignancies in the literature. Based on this limited number of studies, cutaneous metastases from gynecological malignancies are uncommon occurrences. Cutaneous metastases from the synchronous endometrioid carcinoma of the uterine corpus and bilateral ovaries arising from endometriosis are extremely rare. Herein, we report a 51-year-old woman with FIGO Stage 1A Grade 1 endometrial endometrioid-type adenocarcinoma and synchronous bilateral Stage 1B ovarian endometrioid-type adenocarcinoma who presented 34 months following total abdominal hysterectomy and bilateral salpingo-oophorectomy with skin metastases. After the patient underwent an excisional biopsy, we applied a palliative radiotherapy. The patient received the combination therapy with cisplatin and doxorubicin after the completion of radiotherapy but the disease evolution was rapidly fatal and the patient died 4 months after her admission to our department due to widely disseminated disease.Öğe Cutaneous Mucinosis Case with Characteristic Lion Face Appearance(Aves, 2018) Temiz, Selami Aykut; Dursun, Recep; Ataseven, Arzu; Ozer, Ilkay; Findik, SiddikaCutaneous mucinosis includes a heterogeneous group of skin diseases characterized by the deposition of mucin in the interval of the dermis. Mucin is a protein ordinarily found as part of the dermal connective tissues. Mucin is a mucopolysaccharide produced by mast cells and fibroblasts and includes hyaluronic acid and sulfated glycosaminoglycans. Because hyaluronic acid holds water, in disease states where mucin production is increased, the dermal connective tissue swells, which is defined as myxedematous. Systemic mucin deposition may include systemic involvement; monoclonal gammopathy or paraproteinemia have been detected in the large majority (83.2%) of scleromyxedema cases. A 50-year-old female presented with pruritic, flesh-colored papules spread throughout the body. She also exhibited a characteristic lion face appearance. These lesions had first appeared 18 months before in the neck. The protein electrophoresis and bone marrow biopsy of the patient were normal. Histopathological examination revealed widespread mucin accumulation with alcian blue and colloidal iron stains in the papillary and reticular dermis. Granulomas were not observed with CD68 staining, and no accumulation was observed with amyloid staining. The final diagnosis was cutaneous mucinosis. In this paper we present the case of a patient with generalized primary cutaneous mucinosis without any systemic disease.Öğe Diclofenac Sodium Treatment Ameliorates Extrapancreatic Organ Injuries in a Murine Model of Acute Pancreatitis Induced by Caerulein(Hindawi Ltd, 2018) Cakir, Ozlem Ozer; Findik, SiddikaAim. We determined the effects of diclofenac sodium, octreotide, and their combination on extrapancreatic organ injuries in caerulein-induced acute pancreatitis in mice. Methods. A total of 58 BALB-C male mice (25 g) were divided into seven groups and used to create a caerulein-induced acute pancreatitis model. Diclofenac sodium, octreotide, and their combination were given for treatment of caerulin-induced acute pancreatitis in mice. At the end of the experiment, the lung, liver, kidney, and stomach were removed for histopathologic assessment. Results. Histopathologic investigation revealed a statistically significant difference between the groups in mean congestion, edema, tubular injury, perirenal fat tissue inflammation, and tubular stasis scores in kidney tissue (P < 0 001, P < 0 001, P < 0 001, P < 0 001, and P = 0 048, respectively); mean congestion, edema, neutrophil inflammation, mononuclear inflammation, and emphysematous change scores in the lung (P < 0 001, P < 0 001, P < 0 001, P = 0 030, and P < 0 001, respectively); mean congestion, edema, and neutrophil inflammation scores in the stomach (P = 0 008, P = 0 014, and P < 0 001, respectively); and mean congestion and hydropic degeneration scores in the liver (P = 0 029 and P = 0 002, respectively). Conclusion. Diclofenac sodium alone ameliorates lung edema due to caerulin-induced acute pancreatitis.Öğe Effect of methylprednisolone loaded poly lactic-co-glycolic acid (PLGA) bioabsorbable nanofibers on tendon healing and adhesion formation(Elsevier, 2023) Zuhour, Moath; Gunes, Cansu; Findik, Siddika; Dundar, Mehmet Akif; Gok, Orhan; Altuntas, ZeynepBackground: Nanomaterials have been widely used in many fields such as vaccination and drug delivery. Beside its behavior as a degradable physical barrier, it can provide a controlled drug release. Tendon healing is a process full of complications, one of which is adhesion caused by excessive fibrosis.Aim: In this study, we aimed to prevent adhesion formation by using methylprednisolone (MP) loaded Poly lactic-co-glycolic acid (PLGA) mats. We used PLGA mats as both, biodegradable physical barrier to reduce the contact between surrounding tissues and healing tendon and as a drug delivery vehicle to release the antifibrotic MP with a controlled pattern.Methods: MP-loaded PLGA nanofiber mats were produced using electrospinning technique under optimized pa-rameters. To find the optimal MP dose, the drug was loaded in 15%, 25% and 35% concentrations. In vitro analysis included FT-IR, antibacterial, water absorption, biodegradability and drug release behavior tests. 70 rats' tendons were used for this study. After scarification of the rats, tendons were analyzed in terms of macroscopic, histopathological and biomechanical evaluation.Results: The neat PLGA and 15%, 25%, and 35% MP-loaded nanofibers lost 47%, 83%, 88%, and 97% of their initial weights at the end of the 8 weeks' degradation process. Within the first 24 h, mats including higher drug concentrations showed more initial release burst effect than samples loaded with lower concentrations. Cumu-lative drug release at 24 h was 29.5%, 27.6%, and 24.7% for PLGA nanofiber samples containing 15%, 25%, 35% MP, respectively. Macroscopically, When the groups were compared, no statistically significant difference was found between group 1 (no surgical intervention) and group 5 (25% MP/PLGA) in terms of length, characteristics and degree of the adhesion. Comparing to other groups statistically significant atrophic effect was found in group 6 (35% MP/PLGA).Conclusion: 25% MP-loaded PLGA reduces the formation of adhesions macroscopically comparable to tendons that didn't receive any surgical intervention. Microscopically, it provides better tendon healing compared to tendons that received only surgical repair or surgery + neat PLGA. Methylprednisolone did not only add an antibacterial effect to PLGA but also increased the hydrophilic property and degradation rate of PLGA. Increased steroid concentration also leads to atrophy at the healing tendons which can be prevented by modifying the PLGA design.Öğe The Effect of RAS/BRAF Mutation Status on Prognosis and Relapse Pattern in Early Stage Colon Cancers(Springer, 2023) Kunt, Nazli; Araz, Murat; Yildirim, Mahmut Selman; Findik, Siddika; Kocak, Mehmet Zahid; Eryilmaz, Melek Karakurt; Artac, MehmetPurposeIt is known that the RAS and BRAF mutations are predictive for targeted therapies in treating metastatic colon cancer and negatively affect the prognosis of the disease. However, there are limited studies in early-stage colon cancer about the relationship of this mutational condition with the prognosis and relapse pattern of the disease. In this study, we evaluated the effects of mutational status on the clinical pattern of recurrence and survival in early-stage colon cancer in addition to classical risk factors.MethodsPatients with early-stage colon cancer at the first time of diagnosis and developing recurrence or metastasis on following up were included in this study. Patients were divided into two groups according to the at the time of relapse RAS/BRAF mutation status: mutant or non-mutant/wild types. Then, mutation analysis was performed again from the early-stage tissue of the patients if available. The relationship between early-stage mutation status and progression-free survival (PFS), overall survival (OS), and relapse pattern was analyzed.ResultsThe number of patients with mutant and non-mutations in the early stage was 39 and 40, respectively. Mutant and non-mutant patients with stage 3 disease were similar (69% and 70%, respectively). OS (47.27 months vs. 67.53 months; p = 0.02) and PFS (25.12 vs. 38.13 months; p = 0.049) were statistically significantly lower in mutant patients, respectively. Most patients had distant metastases on both sides at recurrence (61.5% vs. 62.5%, respectively). There was no significant difference between mutant and non-mutant patients regarding distant metastasis and local recurrence rates (p = 0.657). A discordance of 11.4% between early-stage and late-stage tissue mutation status.ConclusionThe presence of mutation in early-stage colon cancer is associated with shorter OS and PFS. The mutational status did not have a significant effect on the recurrence pattern. Because of the discordance of early-stage and late-stage mutational status, it is recommended to perform mutation analysis from tissue at relapse.Öğe The effects of a combination treatment with mesenchymal stem cell and platelet-rich plasma on tendon healing: an experimental study(Tubitak Scientific & Technological Research Council Turkey, 2022) Uyar, Ilker; Altuntas, Zeynep; Findik, Siddika; Yildirim, Mehmet Emin Cem; Yarar, Serhat; Aktan, Murat; Avci, AlunetBackground/Aim: The objective of this study was to investigate the effects that the application of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) following tendon repair have on the strength and healing of the tendon and also to examine the possible mechanisms of action that take place. Materials and methods: The Achilles tendons of 80 rats were repaired and divided into eight groups. Following the repairs, MSCs obtained from humans were injected into the rat tendons in groups 1 and 2, a combination of MSCs from humans and PRP from rats was injected into the tendons in groups 3 and 4, and PRP from rats was injected into the tendons in groups 5 and 6. These procedures all took place simultaneously. Groups 7 and 8 did not receive any injections following the repairs. The rats were sacrificed at the end of the first and second months following the procedures, and biomechanical and histopathological analyses were performed. Results: Inflammatory cell density increased most significantly in the combined group when compared to the first and second months. The fibroblast density on the tendon repair region was significantly lower in the second-months groups of each intervention compared to their first-month groups (p = 0.001). For the analysis of the maximum tensile breaking force, the behaviors of the groups over time were significant when compared to the control groups (p = 0.0015). Also, the mean maximum breaking force in the combined group was statistically significantly higher at the end of the second month than at the end of the first month (p = 0.0008). Conclusion: The combination therapy increased tendon strength force. This combination therapy can make a positive contribution to the healing of tendons after surgery.Öğe Epidemiological and Localization Characteristics of Non-Melanoma Skin Cancers: Retrospective Analysis of 400 Cases(Wolters Kluwer Medknow Publications, 2019) Findik, Siddika; Uyanik, Orkun; Altuntas, Mahmut; Altuntas, ZeynepAim: In this study, it was aimed to investigate the epidemiological and localization characteristics of the patients diagnosed with nonmelanocytic skin cancer (NMSC). Materials and Methods: Histopathologic results of patients diagnosed with NMSC in the Pathology Department of Necmettin Erbakan University, Meram School of Medicine, between 2007 and 2017 were retrospectively reviewed. The age, sex, tumor type, and localization of the patients were investigated. Results: A totals of 400 patients diagnosed with NMSC were identified. 220 of the patients were male (55%) and 180 were female (45%). The male to female ratio was 220/180(1.22). The age range was 10-105-year-old and the mean age was 67.8. Basal cell carcinoma (BCC) was found in 263 patients (65%), squamous cell carcinoma (SCC) in 114 patients (28%) and baso SCC (BSCC) in 12 patients (3%). BCC and SCC were observed together in 9 patients. BCC was most commonly detected in the nasal region with (31%) 82 patients (50%). SHC was most commonly detected in the lower lip region with 26 patients (22%). The localization of the BSCC was most common in the nasal region with 6 patients (%50). The rate of BCC/SCC in the nasal region was 82/11. The BCC/SCC ratio in the extremity region was 4/14. Conclusion: In our region, the most frequent histopathologic diagnosis was found BCC in patients prediagnosed with NMSC, and most cases were seen in male patients. In cases of BCC a BSCC, the most common tumor localization was nose area while it was lower lip area in SCC cases. In addition, BCC and BSCC were not detected in the lower lip.Öğe Extended-spectrum of KRAS and NRAS mutations in lung cancer tissue specimens obtained with bronchoscopy(Wolters Kluwer Medknow Publications, 2022) Basdemirci, Muserref; Zamani, Adil; Zamani, Ayse G.; Findik, Siddika; Yildirim, Mahmut S.Background: Mutations in the RAS genes, HRAS, KRAS, and NRAS, are the most common modifications in many types of human tumors and are found in approximately 30% of all human cancers. These mutations are usually found in codons 12, 13, or 61. Methods: The aim of this study is to evaluate mutations in codons 59, 117, and 146 of KRAS and NRAS genes in addition to codons 12,13, and 61 of KRAS gene in lung cancer tissue specimens obtained with bronchoscopy. KRAS and NRAS mutation analyses with pyrosequencing were performed on DNA isolated from formalin-fixed paraffinembedded (FFPE) tissue samples of 64 patients histopathologically diagnosed as lung cancer after bronchoscopic biopsy. Results: In all, 20 patients (31.2%) had mutations in KRAS gene (8/27 squamous cell carcinoma, 8/11 adenocarcinoma, 3/16 small cell carcinoma, and 1/1 pleomorphic carcinoma). The most common mutation in codon 12 was in c.35G>T (G12V). When the mutation rate of adenocarcinoma (72.7%) and squamous cell carcinoma (22.9%) patients was compared with each other, a statistically significant difference was observed (P = 0.008). There were no mutations in codons 59, 117, or 146 of KRAS and NRAS genes in patients with lung cancer. Conclusion: In this study, we firstly examined mutations in codons 59, 117, and 146 of KRAS and NRAS genes in addition to codons 12, 13, and 61 of KRAS gene in Turkish lung cancer patients both in non-small cell lung cancer and small cell lung cancer. Although no mutation was detected in codons 59, 117, and 146 of KRAS and NRAS genes, the frequency of KRAS gene mutation was higher than the rate of mutation in both Asian and Western countries, and multicenter studies including more cases should be performed to further explore our results.Öğe Human papillomavirus (HPV) subtypes and their relationships with cervical smear results in cervical cancer screening: a community-based study from the central Anatolia region of Turkey(E-Century Publishing Corp, 2019) Findik, Siddika; Findik, Serkan; Abuoglu, Seyfettin; Cihan, Fatma Goksin; Ilter, Huseyin; Iyisoy, Mehmet SinanObjective: Cervical cancer can be diagnosed early by cancer screening programs. The aim of this study was to evaluate the cervical smear test results of healthy women. Methods: We enrolled 94,848 healthy women from 30-65 years of age in whom both HPV typing and a cervical smear test was performed between 2014 and 2017. Results: HPV was detected in 3001 women (3.16%). The mean age was 42 +/- 8.94 years old. Positive HPV types were HPV16; HPV16 and multiple infection; HPV31; HPV51; HPV39; HPV52; HPV56; HPV18; HPV68; HPV35; HPV18 and multiple infection; HPV58; HPV45; HPV59; HPV16, HPV18 and multiple infection; HPV16 and 18; and HPV33, in descending order. Cytology results were normal in 63.61%. We also identified atypical squamous cells of undetermined significance in 6.60%, atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesion in 0.73%, low-grade squamous intraepithelial lesion in 10%, high-grade squamous intraepithelial lesion and atypical glandular cells in 0.53%, and adenocarcinoma in situ in 0.03%. In terms of HPV subtypes, abnormal smear results were divided into an HPV16, HPV18, and multiple infection group (25%), an HPV35 group (23.61%), an HPV 16 and multiple infection group (22%), and an HPV16-only group (21.85%). Most cases of HPV (39.02%) and abnormal cytology (43.04%) were detected between the ages of 30 and 40. Conclusions: This study is remarkable because it is a community-based study and includes a large population of healthy women to detect HPV prevalence, its subtype, and abnormal smear results.Öğe Imaging Features of Breast Plasmacytoma(Galenos Publ House, 2022) Babaoglu, Seyma; Poyraz, Necdet; Kaya, Bugra; Findik, Siddika; Demircioglu, Sinan[Abstract Not Availabe]Öğe Immunohistochemical Evaluation of TNF-?, IL-1, IL-12, IL-17, IL-23 Expression and Investigation of the Effect of Demodex in Patients with Discoid Lupus Erythematosus(Galenos Publ House, 2023) Dursun, Recep; Temiz, Selami Aykut; Findik, Siddika; Durmaz, Koray; Oltulu, PembeObjective: Discoid lupus erythematosus (DLE) is a chronic inflammatory skin disease that can be triggered by several factors although its etiology is not yet known. Hypotheses have been reported that the demodex mites may be involved in the etiopathogenesis of DLE. In this study, we aimed to investigate the potential relationship between the frequency of immunohistochemical staining of tumor necrosis factor (TNF)-a, interleukin (IL)-1, IL-12, IL-17 and IL-23 cytokines obtained from cutaneous biopsy of DLE patients and disease severity. Materials and Methods: Biopsy tissues of patients who were previously diagnosed with DLE in the dermatology outpatient clinic, which were also supported histopathologically, were re-sectioned and subjected to immunohistochemical examination for TNF-a, IL-1, IL-12, IL-17 and IL-23, and their staining scores were obtained. These immunohistochemical staining scores were compared with disease severity. The presence and density of demodex were evaluated in standard skin surface biopsy taken from the lesions at the time of diagnosis. Results: In the comparison of immunohistochemical staining scores with DLE-skin score (DLE-SS), a statistically significant positive correlation was found between DLE-SS and TNF-a (p=0.003), DLE-SS and IL-17 (p=0.002). There was no difference between the presence or absence of demodex and DLE-SS (p=0.9). There was no correlation between demodex density and disease severity in demodex-positive cases (p=0.34). Conclusion: In line with the data obtained from our study, TNF-a and IL-17 seem to be more associated with the disease severity in DLE. The fact that demodex positivity/negativity and demodex density are independent of disease severity supports that demodex mite is an etiopathogenetic factor rather than overlap in DLE cases. Further studies on this subject are needed.Öğe The Importance of Preoperative Dermoscopy in Basal Cell Carcinoma Due to a Case of Nodular Cystic BCC(Erciyes Univ Sch Medicine, 2019) Temiz, Selami Aykut; Ozer, Ilkay; Ataseven, Arzu; Dursun, Recep; Findik, SiddikaBasal cell carcinoma (BCC) is the most widespread malignancy among all malignancies. It constitutes 65%-80% of non-melanoma skin malignancies. Although there is no universally accepted classification for BCC, there are at least 26 different defined subtypes, which can all be very difficult to recognize. The best-known variants of BCC are nodular, superficial, morpheaform, and infiltrative types. In this report, we describe a patient who was referred for surgery with the diagnosis of cystic BCC by preoperative dermoscopy. Herein, we present a rare form of nodular cystic BCC due to its importance in improving the knowledge of dermoscopy and surgery.Öğe The Importance of Preoperative Dermoscopy in Basal Cell Carcinoma Due to a Case of Nodular Cystic BCC(Erciyes Univ Sch Medicine, 2019) Temiz, Selami Aykut; Ozer, Ilkay; Ataseven, Arzu; Dursun, Recep; Findik, SiddikaBasal cell carcinoma (BCC) is the most widespread malignancy among all malignancies. It constitutes 65%-80% of non-melanoma skin malignancies. Although there is no universally accepted classification for BCC, there are at least 26 different defined subtypes, which can all be very difficult to recognize. The best-known variants of BCC are nodular, superficial, morpheaform, and infiltrative types. In this report, we describe a patient who was referred for surgery with the diagnosis of cystic BCC by preoperative dermoscopy. Herein, we present a rare form of nodular cystic BCC due to its importance in improving the knowledge of dermoscopy and surgery.Öğe Increased expression of ubiquitin C-terminal hydrolase L1 in astrocytomas of ascending grades(Lippincott Williams & Wilkins, 2023) Izci, Emir Kaan; Keskin, Fatih; Erdi, Fatih; Kaya, Bulent; Karatas, Yasar; Feyzioglu, Bahadir; Findik, SiddikaBackground:The ubiquitin-proteasome pathway controls the monitoring and degradation of important proteins and is involved in several cellular processes, such as development, differentiation, and transcriptional regulation. Recent evidence has shown that ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1), a member of the deubiquitinating enzyme family that removes ubiquitin from protein substrates, is overexpressed in many types of cancer. Aim:This study thus examined the expression of UCH-L1 in human astrocytoma tissues. Material and methods:Formalin-fixed, paraffin-embedded astrocytoma samples were obtained from 40 patients, after which histopathological examination, typing, and grading were performed. The study group included 10 histologically normal brain tissues, which served as the control group, and 10 WHO grade II, 10 WHO grade III, and 10 WHO grade IV (glioblastoma) samples. Normal brain tissue samples were obtained from the histologically normal, non-tumoral portion of the pathology specimens. UCH-L1 expression was evaluated using quantitative reverse transcription-polymerase chain reaction and immunohistochemistry. Results:Astrocytoma tissues exhibited higher UCH-L1 expression compared to the control group. UCH-L1 overexpression increased significantly together with the increase in astrocytoma grades (from II to IV). Conclusion:UCH-L1 could be a good diagnostic and therapeutic marker for determining astrocytoma development and progression.Öğe Intratumoral hemorrhage-related differences in the expression of vascular endothelial growth factor, basic fibroblast growth factor and thioredoxin reductase 1 in human glioblastoma(Spandidos Publ Ltd, 2016) Kaya, Bulent; Cicek, Onur; Erdi, Fatih; Findik, Siddika; Karatas, Yasar; Esen, Hasan; Keskin, FatihThe present study was designed to evaluate the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and thioredoxin reductase 1 (TrxR1) in glioblastoma multiforme (GBM) with and without intratumoral hemorrhage. Surgically resected human GBM samples from 20 patients who underwent surgery at our institute were extracted from the histopathological specimens and divided into two groups. A total of 10 samples from each type of GBM (World Health Organization grade IV, intratumoral hemorrhage-positive or -negative) were included in each group. VEGF, bFGF and TrxR1 expression was analyzed using immunohistochemistry and the results were compared between groups. VEGF and bFGF immunoreactivity was significantly higher in GBMs containing intratumoral hemorrhage. Furthermore, VEGF, bFGF and TrxR1 immunointensity was significantly higher in GBMs containing intratumoral hemorrhage. Thus, the present study demonstrated a higher VEGF, bFGF and TrxR1 expression in GBMs contain intratumoral hemorrhage, indicatiogn a role of VEGF, bFGF and TrxR1 expression in the promotion of tumoral angiogenesis and tumoral growth by complex mechanisms that require further elucidation.Öğe Nicotinamide N-methyltransferase expression in squamous cell carcinoma of the vulva(Taylor & Francis Inc, 2022) Celik, Zeliha Esin; Akar, Serra; Findik, Siddika; Aytekin, Emine; Celik, CetinThe molecular pathways involved in the development of vulvar squamous cell carcinoma (SCC) cancer are not completely known. Nicotinamide N-methyltransferase (NNMT) is a cytosolic enzyme associated with tumorigenesis and metastasis in a variety of cancers. Its role in vulvar cancer has not been studied, previously. Vulvar SCC, high and low grade squamous intraepithelial lesions (SILs) and benign squamous hyperplasia were analysed immunohistochemically. The mean staining score for vulvar SCC was significantly higher than the score for vulvar squamous hyperplasia (p<.001). The mean relapse-free survival for patients with low and high NNMT expression was 41.4 months (95% CI: 25.6-57.2) and 19.8 months (95% CI: 3.0-36.6), respectively (p=.035). The mean disease-specific survival for patients with low and high NNMT expression was 75.8 months (95% CI: 57.5-94.2) and 27.8 months (95% CI 12.2-43.4), respectively (p=.015). Although quite preliminary, this study showed that NNMT expression was elevated in vulvar SCC compared to benign and premalignant lesions. Additionally, elevated NNMT expression was associated with poor survival. Impact Statement What is already known on this subject? Nicotinamide N-methyltransferase (NNMT) is a methyltransferase, associated with tumour progression, spread and poor prognosis in a variety of cancers. Its upregulation can lead to DNA hypomethylation, which can in turn result in the activation of proto-oncogenes and deactivation of tumour suppressor genes. What do the results of this study add? Although quite preliminary, this study showed that NNMT expression was elevated in vulvar SCC compared to benign and premalignant lesions. Additionally, elevated NNMT expression was associated with poor survival. What are the implications of these findings for clinical practice and/or further research? NNMT has been regarded as a potential target of cancer therapy and its role in vulvar cancer has not been studied, previously. This is the first study to investigate the expression of NNMT in vulvar cancer and associate NNMT elevation with poor survival. NNMT can further be investigated as a possible target of vulvar cancer therapy.Öğe Pathology awareness in patients and patient's relatives applying to a pathology laboratory(Saudi Med J, 2019) Findik, Siddika; Cihan, Fatma G.Objectives: To investigate pathology awareness in patients applying to a pathology laboratory. Method: This cross-sectional study included 150 patients and patients' relatives. A questionnaire, prepared by the researchers according to the literature, was administered at the Pathology Laboratory, Necmettin Erbakan University, Konya, Turkey on May 2018. Results: Of the participants, 79 (52.7%) were female and 71 (47.3%) were male. The mean age was 39.56 +/- 14.16 years old. Ninety-eight (65.3%) of them thought that patients do not have right to choose their pathologist. Of the participants, 24 (16%) did not know that the medical school had to be completed in order to become a pathologist and 73 (48.7%) of them did not know that 4 years postgraduate training was required. Fifty-nine (39.3%) of the participants did not know the pathologists were a medical doctor. While 89 participants (59.4%) had no idea what frozen section means, 66 people (44%) did not know that the pathologist uses microscope and 64 (42.7%) of them thought that all diseases could be diagnosed 100% by pathological examination. Forty-six (30.7%) of the participants thought that all specimens are not needed to be examined. Conclusion: The knowledge and awareness of the participants about the pathology discipline came out to be inadequate.
