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Öğe Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D(Walter De Gruyter Gmbh, 2022) Kader, Saadet; Akdag, Turan; Ecer, Busra; Abusoglu, Sedat; Unlu, AliObjectives Vitamin D is one of the major hormones involved in the metabolism of calcium (Ca) and phosphorus (P). In the present study, we aimed to determine the analytical performance of the immunoassay method used for determining plasma 25-hydroxyvitamin D [25(OH)D] levels in routine clinical practice in laboratories. Methods Venous blood samples were collected from 156 patients for the comparisons and were analyzed with Siemens ADVIA Centaur XPT, the Roche Cobas 6,000's module e601, Abbott Architect i2000, and the liquid chromatography with tandem mass spectrometry (LC-MS/MS). Results The four methods were analyzed and compared through the Passing-Bablok regression for 25(OH)D, and the highest correlation was found at LC-MS/MS and Cobas 6,000's module e601 (r=0.799), LC-MS/MS/Abbott Architect i2000, and LC-MS/MS/Siemens ADVIA Centaur XPT as r=0.736, 0.721, respectively. The correlation coefficient was found between Abbott Architect i2000 with Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.934 and r=0.907, respectively. Also, the correlation coefficient was found between Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.906. Conclusions The Roche Cobas assay showed better performance, compared with the other assays. Based on our findings, the chemiluminescence methods in automated systems seem to be expedient.Öğe Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D(Walter De Gruyter Gmbh, 2022) Kader, Saadet; Akdag, Turan; Ecer, Busra; Abusoglu, Sedat; Unlu, AliObjectives Vitamin D is one of the major hormones involved in the metabolism of calcium (Ca) and phosphorus (P). In the present study, we aimed to determine the analytical performance of the immunoassay method used for determining plasma 25-hydroxyvitamin D [25(OH)D] levels in routine clinical practice in laboratories. Methods Venous blood samples were collected from 156 patients for the comparisons and were analyzed with Siemens ADVIA Centaur XPT, the Roche Cobas 6,000's module e601, Abbott Architect i2000, and the liquid chromatography with tandem mass spectrometry (LC-MS/MS). Results The four methods were analyzed and compared through the Passing-Bablok regression for 25(OH)D, and the highest correlation was found at LC-MS/MS and Cobas 6,000's module e601 (r=0.799), LC-MS/MS/Abbott Architect i2000, and LC-MS/MS/Siemens ADVIA Centaur XPT as r=0.736, 0.721, respectively. The correlation coefficient was found between Abbott Architect i2000 with Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.934 and r=0.907, respectively. Also, the correlation coefficient was found between Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.906. Conclusions The Roche Cobas assay showed better performance, compared with the other assays. Based on our findings, the chemiluminescence methods in automated systems seem to be expedient.Öğe Cystatin C as a potential biomarker to evaluate migraine(Assoc Arquivos Neuro- Psiquiatria, 2020) Akdag, Turan; Uca, Ali UlviBackground: Migraine is a multifactorial neurovascular syndrome and closely associated to inflammation. Cystatin C (Cys C) is a neuroendocrine polypeptide which also plays a role in inflammation. Objective: To investigate the levels of Cys C in migraine patients without aura. Methods: A total of 80 participants were included in the study; 40 patients and 40 healthy controls. Serum Cys C levels were investigated by using enzyme-linked immunosorbent assay (ELISA). Statistical analysis were performed using Statistical Package for the Social Sciences (SPSS) version 22.0 (SPSS Inc, IL, USA). Results: Serum Cys C levels were found as 73.88 ng/mL in the patient group and 24.92 ng/mL in the healthy control group, being significantly higher among patients (p=0.000). Serum Cys C levels were significacntly different across age subgroups among patients (p=0.049), but not among controls. However, visual analog scale (VAS) (p=0.707), disease duration time (p=0.725) and body mass index (p=0.136) were not significantly different between the two groups. Conclusion: Our findings demonstrate that high serum Cys C levels are independently associated to migraine without aura. To the best of our knowledge, this is the first study to determine the serum levels of Cys C in patients with migraine. Thus, serum Cys C may be a potential biomarker of migraine.Öğe Cystatin C as a potential biomarker to evaluate migraine(Assoc Arquivos Neuro- Psiquiatria, 2020) Akdag, Turan; Uca, Ali UlviBackground: Migraine is a multifactorial neurovascular syndrome and closely associated to inflammation. Cystatin C (Cys C) is a neuroendocrine polypeptide which also plays a role in inflammation. Objective: To investigate the levels of Cys C in migraine patients without aura. Methods: A total of 80 participants were included in the study; 40 patients and 40 healthy controls. Serum Cys C levels were investigated by using enzyme-linked immunosorbent assay (ELISA). Statistical analysis were performed using Statistical Package for the Social Sciences (SPSS) version 22.0 (SPSS Inc, IL, USA). Results: Serum Cys C levels were found as 73.88 ng/mL in the patient group and 24.92 ng/mL in the healthy control group, being significantly higher among patients (p=0.000). Serum Cys C levels were significacntly different across age subgroups among patients (p=0.049), but not among controls. However, visual analog scale (VAS) (p=0.707), disease duration time (p=0.725) and body mass index (p=0.136) were not significantly different between the two groups. Conclusion: Our findings demonstrate that high serum Cys C levels are independently associated to migraine without aura. To the best of our knowledge, this is the first study to determine the serum levels of Cys C in patients with migraine. Thus, serum Cys C may be a potential biomarker of migraine.Öğe Elevated levels of neopterin and pentraxin 3 in patients with rheumatoid arthritis(Walter De Gruyter Gmbh, 2021) Ekin, Sabri; Sivrikaya, Abdullah; Akdag, Turan; Yilmaz, Sema; Gulcemal, SemralObjectives: As a systemic inflammatory disease, rheu-matoid arthritis (RA) is the most common inflammatory arthritis in the population and there is no specific diag-nostic marker in laboratory tests. The purpose of the study was to determine whether serum neopterin and pentraxin 3 (PTX3) levels may be a marker of increased inflammation in RA patients. Materials and methods: The study were consist of 30 RA patients and 30 healthy controls who were admitted to the department of rheumatology. Blood specimens were taken from both group, and the levels of neopterin were analyzed by chromatography method (HPLC) and the PTX 3 levels were measured by enzyme-linked immunosorbent assay (ELISA). All data and demographic characteristics of par-ticipants were also recorded. Results: Serum neopterin and PTX 3 levels of the patient group (25.99 +/- 7.24 ng/mL and 4.19 +/- 1.01 ng/dL, respec-tively) was higher than the control group (9.55 +/- 0.74 ng/mL and 2.23 +/- 0.39 ng/dL, respectively). These results were remarkable significant (p<0.01). No statistically significant correlation was found between age-PTX 3, age-neopterin and PTX 3-neopterin parameters in the patient group. In the control group, a significant negative correlation was found between age and PTX 3 (p<0.05), and a positive correlation between neopterin and PTX 3. Conclusions: Consequently, the serum neopterin and PTX 3 levels were higher in RA patients as compared to the healthy individuals. Our study suggest that there is a relation between neopterin and PTX 3 levels with RA patients. These findings suggest that neopterin and PTX 3 are important markers in the monitoring of RA disease.Öğe Elevated levels of neopterin and pentraxin 3 in patients with rheumatoid arthritis(Walter De Gruyter Gmbh, 2021) Ekin, Sabri; Sivrikaya, Abdullah; Akdag, Turan; Yilmaz, Sema; Gulcemal, SemralObjectives: As a systemic inflammatory disease, rheu-matoid arthritis (RA) is the most common inflammatory arthritis in the population and there is no specific diag-nostic marker in laboratory tests. The purpose of the study was to determine whether serum neopterin and pentraxin 3 (PTX3) levels may be a marker of increased inflammation in RA patients. Materials and methods: The study were consist of 30 RA patients and 30 healthy controls who were admitted to the department of rheumatology. Blood specimens were taken from both group, and the levels of neopterin were analyzed by chromatography method (HPLC) and the PTX 3 levels were measured by enzyme-linked immunosorbent assay (ELISA). All data and demographic characteristics of par-ticipants were also recorded. Results: Serum neopterin and PTX 3 levels of the patient group (25.99 +/- 7.24 ng/mL and 4.19 +/- 1.01 ng/dL, respec-tively) was higher than the control group (9.55 +/- 0.74 ng/mL and 2.23 +/- 0.39 ng/dL, respectively). These results were remarkable significant (p<0.01). No statistically significant correlation was found between age-PTX 3, age-neopterin and PTX 3-neopterin parameters in the patient group. In the control group, a significant negative correlation was found between age and PTX 3 (p<0.05), and a positive correlation between neopterin and PTX 3. Conclusions: Consequently, the serum neopterin and PTX 3 levels were higher in RA patients as compared to the healthy individuals. Our study suggest that there is a relation between neopterin and PTX 3 levels with RA patients. These findings suggest that neopterin and PTX 3 are important markers in the monitoring of RA disease.Öğe Elevated survivin levels in patients with acne vulgaris(Wiley, 2022) Aksoy Sarac, Gulhan; Kader, Saadet; Akdag, TuranObjective Acne vulgaris is a multifactorial disease of the pilosebaceous unit. As the most common skin disease, it may affect approximately 85% of the young population. Survivin, a member of the inhibitors of the apoptosis (IAP) gene family, can inhibit apoptosis and regulate cell division and proliferation. In the study, we aimed to investigate the potential role of serum survivin in acne vulgaris. Methods Forty individuals who were diagnosed with acne vulgaris and forty healthy subjects as the control group were enrolled in the study. Venous blood samples were collected from each participant, and the serum levels of survivin were measured by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using SPSS software version 25. Results The serum survivin levels were statistically significant between the groups, and the levels of survivin were measured as acne vulgaris patients group 153.44 and control group 104.17 pg/ml, respectively (p < 0.018). When the serum survivin levels were compared according to gender, females had higher levels of survivin than the males (168.16 versus 50.45 pg/mL, p = 0.001). A significant correlation was found between acne severity (p = 0.017) and Scale for Acne Scar severity (SCAR-S) score (p = 0.001) according to the survivin levels. In terms of age, no significant relationship was found between age and survivin (p = 0.4048). Conclusion Elevated serum levels of survivin were determined in acne vulgaris. Moreover, there was a significant correlation between acne stage and SCAR-S score according to survivin levels.Öğe Evaluation of BD Vacutainer Eclipse and BD Vacutainer Ultra-Touch butterfly blood collecting sets in laboratory testing(Walter De Gruyter Gmbh, 2021) Kader, Saadet; Kirmit, Adnan; Akdag, Turan; Tekindal, Mustafa AgahObjectives Choosing the right device for blood collection is an issue that is frequently encountered in laboratory medicine. The purpose of this study was to compare the quality of the samples collected in terms of hemolysis index, pain intensity and tube filling rates of the samples drawn with BD Vacutainer(R) EclipseNeedle and BD Vacutainer Ultra-Touch (TM) Butterfly Blood Collection Set, which is currently used in laboratories. Methods Blood samples were drawn from the 44 apparently healthy adult individuals from different arm sites by routine phlebotomy into Citrate, SST and EDTA tubes in Karapinar State Hospital. K, P, AST, LDH tests and hemolysis index were analyzed by Mindray BS-800 chemistry analyzer. Complete blood count (CBC) was determined by Mindray BC 6000. Results There was no significant difference in K, P, AST, LDH and CBC values between the devices for hemolysis (p>0.05). No significant difference was found out with respect to visual hemolysis and hemolysis index, tube filling speed and pain intensity between the devices. Conclusions Our findings demonstrate that two sets may used for venipuncture blood collection without creating additional hemolysis risk.Öğe Evaluation of BD Vacutainer Eclipse and BD Vacutainer Ultra-Touch butterfly blood collecting sets in laboratory testing(Walter De Gruyter Gmbh, 2021) Kader, Saadet; Kirmit, Adnan; Akdag, Turan; Tekindal, Mustafa AgahObjectives Choosing the right device for blood collection is an issue that is frequently encountered in laboratory medicine. The purpose of this study was to compare the quality of the samples collected in terms of hemolysis index, pain intensity and tube filling rates of the samples drawn with BD Vacutainer(R) EclipseNeedle and BD Vacutainer Ultra-Touch (TM) Butterfly Blood Collection Set, which is currently used in laboratories. Methods Blood samples were drawn from the 44 apparently healthy adult individuals from different arm sites by routine phlebotomy into Citrate, SST and EDTA tubes in Karapinar State Hospital. K, P, AST, LDH tests and hemolysis index were analyzed by Mindray BS-800 chemistry analyzer. Complete blood count (CBC) was determined by Mindray BC 6000. Results There was no significant difference in K, P, AST, LDH and CBC values between the devices for hemolysis (p>0.05). No significant difference was found out with respect to visual hemolysis and hemolysis index, tube filling speed and pain intensity between the devices. Conclusions Our findings demonstrate that two sets may used for venipuncture blood collection without creating additional hemolysis risk.Öğe Evaluation of D-Dimer and Neutrophil/Lymphocyte Ratios of COVID-19 Patients Whom Applied to Karapinar State Hospital(Marmara Univ, Inst Health Sciences, 2022) Kader, Saadet; Akdag, Turan; Sariyildiz, Levent; Ozel, ZeynepObjective: The epidemic which caused by the SARS-CoV-2 virus were defined as COVID-19) and declared as a global pandemic by the World Health Organization (WHO) on March, 2020. Nowadays, many biochemical parameters related to the diagnosis and prognosis of COVID-19 are being investigated. Therefore, we aimed to evaluate D-dimer and neutrophil/lymphocyte ratios (NLR) of COVID-19 patients whom applied to Karapinar State Hospital. Methods: Patients which consisted of 2523, whom diagnosed with COVID-19 between 11 March 2019 and 29 July 2021 at Karapinar State Hospital were included in the study. Age, gender and social history of the patients were recorded. From the results, the relationships between D-dimer and hemogram were evaluated. Results: There was a high correlation between the variables HCT and HGB, PLT and PCT, NEUT# and WBC, and MCH and MCV (r=0.981, r=0.944, r=0.923, r=0.925). In addition, there was a high correlation between RBC and HCT and between RBC and HGB variables (r=0.852, r=0.795). There was a moderate correlation between WBC and MO#, MCHC and MCH, PDW and MPV (r=0.562, r=0.639, r=0.64). All the relationships between these variables were positive, and the value of the correlated parameter increases linearly by unit. Also, the highest positive relationships were between HCT and HGB, PLT and PCT, NEUT# and WBC, MCH and MCV. Moreover, D-dimer and NLR were not correlated (r= - 0.015, p=0.49). Conclusion: In the study, no correlation was observed between D-dimer and neutrophil/lymphocyte ratios of COVID-19 patients. So, more comprehensive and further studies are needed to clarify these results.Öğe Evaluation of diagnostic performance of haematological parameters in Behcet's disease(Wiley-Hindawi, 2021) Tezcan, Dilek; Korez, Muslu Kazim; Gulcemal, Semral; Hakbilen, Selda; Akdag, Turan; Yilmaz, SemaObjective Behcet's Disease (BD) is a polygenic and chronic autoinflammatory multisystemic vasculitis disease characterised by mucocutaneous, musculoskeletal, neurological, gastrointestinal and ophthalmologic lesions. There has been no specific test or serum marker to measure and determine the diagnosis and severity of BD. Purpose The study aimed to investigate the diagnostic performance of haematological parameters as MLR (monocyte to lymphocyte ratio), NLR (neutrophil to lymphocyte ratio), PLR (platelet to lymphocyte ratio), MPV (mean platelet volume), MPVPR (mean platelet volume to platelet ratio), LMR (lymphocyte to monocyte ratio), LPM (lymphocyte and platelet multiplication), WLP (lymphocyte and leukocyte multiplication), RDW (red blood cell distribution width) and PCT (plateletcrit) in BD and compare these with disease activity and clinical findings. Methods A total of 266 participants (49 healthy control and 217 BD patients) were recruited from the rheumatology department in a single-centre as a case-control study. The laboratory data were obtained from the electronic registration database. BD Activity scores (BDCAF/Behcet's Disease Current Activity Form) were calculated. Laboratory findings of BD patients and healthy controls were compared and evaluated. Results RDW, Platelet, PCT, NLR and PLR values were significantly higher in patient group than in the healthy controls. However, haemoglobin, MPVPR and LMR were significantly lower in the patient group which compared with the healthy controls. LPM in BD with genital ulcers, WLP in BD with genital ulcers and arthritis, MPR in BD with uveitis, RDW in BD with thrombosis and neuro-Behcet's disease (NBD), PLR in NBD were observed to be higher. However, LMR in NBD and MPV in BD with thrombosis were lower than those without. There was a positive correlation between BDCAF score and RDW, and NLR. Conclusion Haemoglobin, RDW, Platelet, PCT, NLR, LMR, PLR and MPVPR were statistically significant predictors for BD. RDW, PCT and NLR are the most valuable predictors for BD.Öğe Evaluation of MCV/RDW Ratio and Correlations With Ferritin in Telogen Effluvium Patients(Mattioli 1885, 2022) Ibis, Saadet; Sarac, Gulhan Aksoy; Akdag, TuranIntroduction: Telogen effluvium is one of the chronic diseases that affect the quality of life (QoL) in women. Genetic factors, vitamin deficiencies, hormonal and environmental conditions take roles in the etiology of hair loss. Objectives: The study aimed to evaluate the RDW(Red cell distribution witdh)/MCV(Mean corpuscular volume) ratio and its correlation with ferritin in Telogen Effluvium patients and to reveal their potential role in the etiopathogenesis of Telogen effluvium. Methods: We retrospectively evaluated the medical data of 250 patients who were admitted to the dermatology outpatient clinic between September 2020 and December 2020 with a diagnosis of telogen effluvium. The control group was created retrospectively from the medical records of 250 healthy individuals. HB(Hemoglobin), HCT(Hematocrit), MPV(mean platelet volume), MCV, RDW, ferritin, and MCV/RDW ratio of both groups were compared and evaluated statistically. Results: All telogen effluvium patients were women in terms of gender. The mean age of the patient group was 33.11 +/- 9.66 years and the mean age of the control group was 34.98 +/- 12.37 years. The ratio of MCV/RDW, MPV, MCV, and ferritin is lower in the group with telogen effluvium compared to the control group and a statistically significant difference was found (P < 0.05). Conclusions: Iron deficiency anemia is thought of as a factor in female patients with telogen effluvium. Although the data shows the correction of iron deficiency is insufficient telogen effluvium, we proposed that laboratory tests should be routinely used in the diagnosis and treatment phase of patients who apply with the complaint of hair loss.Öğe Evaluation of serum interleukin-6 (IL-6), IL-13, and IL-17 levels and computed tomography finding in interstitial lung disease associated with connective tissue disease patients(Springer London Ltd, 2021) Tezcan, Dilek; Sivrikaya, Abdullah; Ergun, Dilek; Ozer, Halil; Eryavuz Onmaz, Duygu; Korez, Muslu Kazim; Akdag, TuranObjective: Interstitial lung disease (ILD) is one of the most severe complications which is associated with connective tissue disease (CTD) and causes to morbidity and mortality. So, we aimed to determine serum levels of interleukin-6 (IL-6), IL-13, and IL-17, to investigate whether these cytokines are related to CTD-ILD, and to find their possible contribution to determining the prognosis of the disease. Methods: A total of 150 participants, 80 patients diagnosed with CTD-ILD (mean age, 58.21 +/- 12.36) and 70 healthy controls (mean age, 57.07 +/- 9.60) were recruited from the rheumatology department between January 2016 and June 2019 in the study. High-resolution computed tomography (HRCT) findings were scored as similarly to previous studies. Serum IL-6, IL 13, and IL-17 levels were measured by ELISA test kits. Results: The levels of IL-6, IL-13, and IL-17 in CTD patients were significantly higher than the healthy individuals (p < 001), but the HRCT score's relation were not determined. IL-6 was associated with disease duration and disease activity scores of DAS28, ESDAII, and dSSc. There was a significant relation between dSSc, HCRT fibrosis, and total score. CRP, hemoglobin, and platelets were associated with the HRCT inflammation pattern. Conclusion: At the study, it has been observed that serum IL-13, IL-6 and IL-17 levels are increased in patients with CTD-ILD. Besides, IL-6 was associated with disease activity scores of DAS28, ESDAII, and dSSc. Also, HRCT fibrosis score is associated with dSSc. Further and comprehensive studies are needed to understand better the complex intersection of lung disease with systemic autoimmunity.Öğe Evaluation of the association between platelet tests and thyroid-stimulating hormone levels in patients with vitiligo(Wiley, 2022) Aksoy Sarac, Gulhan; Ibis, Saadet; Akdag, TuranObjective Vitiligo is a common dermatological disease of unknown cause and progressing with depigmentation and affects approximately 1% of the world population. In the study, we aimed to compare plateletcrit (PCT), mean platelet volume (MPV), platelet (PLT), and thyroid-stimulating hormone (TSH) values in vitiligo patients. Material and Methods We retrospectively evaluated the medical data of 100 patients who were admitted to the dermatology outpatient clinic between January 2020 and December 2021 with a diagnosis of vitiligo. The control group was retrospectively constituted from medical records of 90 healthy individuals. PCT, MPV, PLT, and TSH levels of both groups were compared statistically. Results A total of 190 participants (100 vitiligo patients and 90 healthy volunteers) were included in the study. The mean age of the patient group was 38.62 +/- 1.62, while the mean age of the control group was 41.52 +/- 1.54. There were no differences between the two groups in terms of age and gender. It was found that the mean MPV value in the patient group was lower than the control group (p = 0.00). PLT and PCT values were significantly higher in the patient group than the control group (p = 0.00, p = 0.01, respectively). There was no statistically significant difference between the two groups in terms of TSH (p > 0.05). A negative correlation between MPV and PLT values in the patient group (r = -0.218, p = 0.029), and a negative correlation between MPV and TSH (r = -0.218, p = 0.029), (-0.230, p = 0.021). Conclusion In the study, a comparison of the PCT, MPV, and PLT levels showed a difference between both groups, but no differences in TSH levels. To clarify these results, comprehensive studies with more samples are needed.Öğe Investigation of serum adropin levels and its relationship with hypothalamic atrophy in patients with multiple sclerosis(Elsevier Sci Ltd, 2022) Demirdogen, Filiz; Akdag, Turan; Gunduz, Zahide Betuel; Odabas, Faruk OmerObjective Adropin is expressed in vascular endothelial cells and regulates nitric oxide (NO) bioavailability by upregulating nitric oxide. In recent years, some studies have revealed its relationship with the pathogenesis of multiple sclerosis (MS). Our aim in this study is to determine serum adropin levels in MS patients and to investigate adropin levels's relationship with hypothalamic atrophy.Methods A total of 80 people, 40 of whom had MS and 40 of whom were healthy volunteers, were included in the study. Serum samples were taken from all participants. Hypothalamus and pituitary diameters were calculated from magnetic resonance imaging of MS patients. The relationship between serum adropin levels and demographic characteristics, Expanded Disability Status Scale (EDSS), and hypothalamic atrophy were evaluated.Results The levels of adropin were 0.85 +/- 0.14 ng/mL in patients with MS and 2.96 ng/mL +/- 0.285 ng/mL in the healthy controls. MS patients had significantly lower levels of adropin than the healthy controls (p = 0.003). Adropin has the highest diagnostic value (AUC=0.874, (95% CI, 0,800-0,947) as cut-off value (838.00), sensitivity (80.43%) and specificity (70.64%) in the MS group. In the study, serum adropin levels were not significantly correlated with 3 ventricle diameter (3VD) and pituitary diameter (PD) size (p = 0,968) and no significant relationships were determined between adropin and other clinical parameters.Conclusion As a potential diagnostic marker, adropin levels were significantly lower in MS patients than in those without. Comprehensive studies are needed to verify this entity.Öğe Investigation of Some Hematological and Biochemical Parameters in Pregnancy and Early Puerperium(Derman Medical Publ, 2013) Sariyildiz, Levent; Akdag, Turan; Tiftik, Ali MuhtarAim and Material and Method: The aim of the study was investigate some biochemical parameters in pregnancy and after birth. In this study, we determined the levels of serum Fe, TIBC, Ca, Mg, triglyceride, total cholesterol, and whole blood WBC, RBC, HCT, HGB in twenty pregnant women whose age ranges were 20-35 yr. The samples were collected before the birth for three times (during three trimester) and after the forty days of birth for only once. Results: Our findings show that levels of triglyceride, total cholesterol and RBC statistically increased (p<0.01) in pregnant women from the first trimester to the birth. Levels of RBC statistically (p<0.01) decreased more in the forth day after the birth than the first trimester of pregnancy. Although we determined statiscally important low levels of triglyceride, total cholesterol after the birth, we also concluded that those low levels were higher than those at the beginning of pregnancy. Although our findings showed statistically important low levels of Fe, TIBC, Ca and Mg since the first trimester during pregnancy, Ca and Mg levels increased more in the forth day after birth than those at the beginning of pregnancy. Furthermore, Fe levels showed an important increase (p<0.05) but did not reach the levels that were at the beginning of pregnancy. In the second trimester an increase and in the third trimester, in the forth day of the birth decreases in TIBC levels were determined. At the end of the pregnancy, the lowest levels of TIBC were determined. We concluded statistically important (p<0.01) low levels of RBC, HGB and HCT in the second trimester. However, it reached the levels that were at the beginning of pregnancy in the third trimester. Whereas the levels of RBC and HGB in the forth day of the birth were higher than (p<0.01) those at the first trimester, hematocrit levels were similar to the level that were at the beginning of pregnancy. Discussion: Metabolic change that occur during pregnancy affect the health of both mother and fetus. Before and during pregnancy routine investigation and monitoring of the biochemical parameters will be important for prevention of deficiency for the healthy pregnancy and fetus.Öğe A New Predictor AMH (anti-mullerian hormone) to Determining Ovarian Reserve and Menoposual Aging in the Women(Derman Medical Publ, 2013) Sariyildiz, Levent; Akdag, TuranAnti-mullerian hormone (AMH), also termed Mullerian inhibiting substance (MIS), is produced in ovary by granulose cells and known as a member of the TGF-beta superfamily. The researchers imply that AMH is associated with the various growth factors in development and growth of ovarian follicles, furthermore is exhibited the features of other TGF-beta family members. AMH has a critical importance in reproductive age of women. Certain standardization has not yet been developed in determing the ovarian reserve. Recent findings reveal the importance of AMH in determining ovary function and, highlight the importance of AMH than FSH.Öğe Possible roles of sestrin2 in multiple sclerosis and its relationships with clinical outcomes(Assoc Arquivos Neuro- Psiquiatria, 2022) Odabas, Faruk Omer; Uca, Ali Ulvi; Akdag, Turan; Demirdogen, Filiz; Altas, Mustafa; Tokgoz, Osman SerhatBackground: Characterized by demyelination, inflammation and axonal damage, multiple sclerosis (MS) is one of the most common disorders of central nervous system led by the immune system. There is an urgent and obvious need for biomarkers for the diagnosis and follow-up of MS. Objective: To investigate serum levels of sestrin2 (SESN2), a protein that responds to acute stress, in MS patients. Methods: A total of 85 participants, 40 patients diagnosed previously with relapsing-remitting MS and 45 healthy controls, were included. Serum SESN2 parameters were investigated in blood samples drawn from each participant in the patient and control groups. Results: SESN2 levels were significantly lower in MS patients than in controls (z:-3.06; p=0.002). In the ROC analysis of SESN2, the predictive level for MS was 2.36 ng/mL [sensitivity, 72.50%; specificity, 55.56%; p=0.002; area under the curve (AUC)=0.693]. For the cut-off value in both groups, SESN2 was an independent predictor for MS [Exp (B)=3.977, 95% confidence interval (95%CI) 1.507-10.494 and p=0.013]. Conclusions: The decreased expression of SESN2 may play a role in MS pathogenesis, and SESN2 could be used as a biomarker for MS and as immunotherapeutic agent to treat MS.Öğe Possible roles of sestrin2 in multiple sclerosis and its relationships with clinical outcomes(Assoc Arquivos Neuro- Psiquiatria, 2022) Odabas, Faruk Omer; Uca, Ali Ulvi; Akdag, Turan; Demirdogen, Filiz; Altas, Mustafa; Tokgoz, Osman SerhatBackground: Characterized by demyelination, inflammation and axonal damage, multiple sclerosis (MS) is one of the most common disorders of central nervous system led by the immune system. There is an urgent and obvious need for biomarkers for the diagnosis and follow-up of MS. Objective: To investigate serum levels of sestrin2 (SESN2), a protein that responds to acute stress, in MS patients. Methods: A total of 85 participants, 40 patients diagnosed previously with relapsing-remitting MS and 45 healthy controls, were included. Serum SESN2 parameters were investigated in blood samples drawn from each participant in the patient and control groups. Results: SESN2 levels were significantly lower in MS patients than in controls (z:-3.06; p=0.002). In the ROC analysis of SESN2, the predictive level for MS was 2.36 ng/mL [sensitivity, 72.50%; specificity, 55.56%; p=0.002; area under the curve (AUC)=0.693]. For the cut-off value in both groups, SESN2 was an independent predictor for MS [Exp (B)=3.977, 95% confidence interval (95%CI) 1.507-10.494 and p=0.013]. Conclusions: The decreased expression of SESN2 may play a role in MS pathogenesis, and SESN2 could be used as a biomarker for MS and as immunotherapeutic agent to treat MS.Öğe Serum levels of irisin and nesfatin-1 in multiple sclerosis(Assoc Arquivos Neuro- Psiquiatria, 2022) Altas, Mustafa; Uca, Ali Ulvi; Akdag, Turan; Odabas, Faruk Omer; Tokgoz, Osman SerhatBackground: Multiple sclerosis (MS) is an inflammatory and neurodegenerative autoimmune chronic neurological disease. Currently, there are no effective serum biomarkers to verify MS diagnosis, to assess disease prognosis, and evaluate response to MS treatment. Objective: The present study is a preliminary assessment of irisin and nesfatin-1 serum levels in patients with relapsing-remitting MS (RRMS). Methods:A total of 86 participants, 42 patients with RRMS diagnosis and 44 healthy controls were included in the study. The serum irisin and nesfatin-1 parameters of the patients and control group members were analyzed.Results:Irisin and nesfatin-1 levels of the RRMS patients were significantly lower than the controls (z:-3.82, p<0.001; z:-4.79, p<0.001, respectively) The cut-off level of irisin is 10.390 (ng/mL) (sensitivity: 84.1%, specificity: 71.4%, AUC: 0.800), and the cut-off level of nestatin-1 is 7.155 (ng/mL) (sensitivity: 68.2%, specificity: 64.3%, AUC: 0.739) in the ROC analysis. For these cut-off levels in the case-control groups, the lower irisin and nesfatin-1 levels are the independent variables for MS patients (OR 9.723, 95%CI 2.884-32.785, p<0.001; OR 3.992, 95%CI 1.336-11.928, p<0.001) respectively. Conclusion: The present study revealed lower irisin and nesfatin-1 levels in patients with RRMS. These findings suggest that the decreased levels of irisin and nesfatin-1 peptides may contribute to MS pathogenesis such as inflammation, oxidative stress, and apoptosis in MS, leading to demyelination, axonal damage with neuronal loss, and gliosis.
