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Öğe Borik Asitin Kök Dentin Üzerine Etkisi Mineral İçeriği ve Bağ Dayanımı AH-Plus: SEM-EDX Çalışması(2016) Akman, Melek; Belli, Sema; Olcay, Keziban; Özçopur, BetülBorik asit, EDTA ve sitrik asitin kök dentininin mineral içeriğine ve AH Plus’ın makaslama bağlanma dayanımına etkisini araştırmaktır. Gereç ve Yöntemler: 18 adet çekilmiş premolar dişler kullanıldı. Dişlerin kuron kısımları kaldırıldı. Herbir dişten iki kök yarısı elde etmek için su soğutması altında uzunlamasına kesit alındı. SEM EDX kullanılarak element seviyeleri ölçüldü. AH Plus ile build-up oluşturuldu. Element seviyeleri ve makaslama bağlanma dayanım değerleri (MPa) kaydedildi. Shear testi yapıldıktan sonra kök yüzeyleri tekrar düzleştirildi ve %5,25 NaOCl’u takiben %10 Borik asit, %17 EDTA and %10 Sitrik asit uygulandı. Kök yüzeyleri tekrar SEM EDX ile ölçüldü. Element seviyeleri ve makaslama bağlanma dayanım değerleri kaydedildi (MPa) ve paired t testi kullanılarak analiz edildi. Bulgular: Borik asit uygulanımı kök dentininin O, Na, K ve Ca/P oranını (p<0.001) artırdı, Ca, C, Mg mineral içeriğini (p>0.05) değiştirmedi. EDTA uygulanımı O, Na, C, Ca/P oranını artırdı (p<0.001), Ca, P, S mineral içeriğini azalttı (p<0.001). Sitrik asit uygulanımı C, Na, Ca/P oranını artırdı (p<0.001), Ca, P, S mineral içeriğini azalttı (p<0.05). Sitrik asit uygulanımı AH Plus’ın makaslama bağlanma dayanım değerini değiştirmedi (p=0.218). Hem Borik asit hem de EDTA uygulanımı AH Plus’ın makaslama bağlanma dayanım değerini azalttı (p=0.000). Sonuç: Tüm test edilen materyaller kök dentininin mineral içeriğini değiştirdi. Borik asit ve EDTA AH Plus’ın makaslama bağlanma dayanımını azalttı. Borik asit alternatif şelasyon ajanı olarak düşünülebilir ancak materyalin kullanım güvenilirliği için daha ileri çalışmalara ve klinik uygulamalara ihtiyaç vardır.Öğe Comparison of Different Irrigation Activation Regimens and Conventional Irrigation Techniques for the Removal of Modified Triple Antibiotic Paste from Root Canals(Elsevier Science Inc, 2015) Akman, Melek; Akbulut, Makbule Bilge; Aydinbelge, Hale Ari; Belli, SemaIntroduction: The aim of this study was to compare the efficacy of irrigation activation regimens and conventional syringe irrigation technique in the removal of modified triple antibiotic paste (mTAP) from root canal walls. Methods: Fifty-six extracted human mandibular premolars were prepared using ProTaper rotary files (Dentsply Maillefer, Ballaigues, Switzerland) up to size F4. The root canals were filled with mTAP medicament, and after 21 days, the roots were randomly assigned to 5 groups (n = 10) according to the irrigation regimens used: conventional syringe irrigation (Cl), Self-Adjusting File (SAF; ReDent-Nova, Ra'anana, Israel), EndoVac (Discus Dental, Culver City, CA), EndoActivator (Dentsply, Tulsa, OK), and passive ultrasonic irrigation (PUI). In 3 teeth, mTAP was not removed (positive controls), and another 3 teeth were not filled with mTAP (negative controls). The roots were sectioned, and the amount of remaining medicament at each root half (n = 20) was evaluated at 30x magnification using a 4-grade scoring system. Data were evaluated using the Kruskal-Wallis and Mann-Whitney U tests. Results: There were statistically significant differences among all experimental groups; of which, the Cl group was the significantly least efficient in removing mTAP from the root canal (P < .05). At the apical and middle third, the PUI, SAF, EndoVac, and EndoActivator groups removed significantly more mTAP medicament than the Cl group (P < .05); however, there were no significant differences between these groups (P > .05). The SAF and PUI showed significantly better performances in removing mTAP from the coronal third (P < .05). Conclusions: The use of irrigation activation regimens significantly improves the removal of mTAP from root canals when compared with Cl.Öğe The Effect of Different Fiber Post-Application Techniques on Fracture Resistance of Structurally Compromised Premolars with Flared Root Canals: An In Vitro Study(Sage Publications India Pvt Ltd, 2022) Kanyilmaz, Ata Nisa Coban; Akman, Melek; Sismanoglu, Soner; Belli, SemaAim: Intraradicular post systems have become an important treatment method for endodontically treated teeth as they provide retention for restoration to the remaining tooth structure. The objective of the present in vitro study was to evaluate the effect of different fiber post-application techniques on the fracture resistance of premolars with flared root canals and no ferrule. Materials and Methods: Sixty freshly extracted, single-rooted mandibular premolars with no ferrule were endodontically treated by an experienced endodontist, and their root canals were experimentally flared. The specimens with flared root canals were divided into five groups according to fiber post-application techniques (n = 12): a prefabricated fiber post (G1), G1 + two auxiliary posts (G2), i-TFC post system (G3), G1 + quartz splint (G4), and Ribbond (G5). The data were analyzed with analysis of variance and Tukey tests. Results: The findings were revealed that no significant difference was found among four groups, except G5 (P > .05), which demonstrated the lowest fracture resistance (P < .05). G2, G3, G4, and G5 exhibited dominantly restorable fracture pattern, whereas G1 exhibited nonrestorable fracture pattern. Conclusion: Within the limitations of this study, the placement of auxiliary posts, i-TFC post system, quartz splint placement, and Ribbond increased the incidence of restorable fracture compared with the controls.Öğe Effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with Bioaggregate apical plugs(Taylor & Francis Ltd, 2016) Akman, Melek; Akbulut, Makbule Bilge; Guneser, Mehmet Burak; Eldeniz, Ayce UnverdiTo evaluate the effect of intracanal medicaments on the push-out bond strength of Biodentine in comparison with DiaRoot BioAggregate (BA) when used as apical plugs. Forty single-rooted teeth were prepared using Peeso reamers. The samples were divided into four groups. The intracanal medicaments were applied to the root canals as follows: Group1: a combination of metronidazole-ciprofloxacin-cefaclor, Group2: a combination of metronidazole-ciprofloxacin, Group3: calcium hydroxide, and Group4: no medication. After 21 days, the medicaments were removed. The apical part of each root was horizontally sectioned into 1-mm thick slices. The samples were divided into two subgroups, and the following materials were placed: Biodentine, DiaRoot-BioAggregate. After 48-h incubation, the push-out bond strength was measured. The data were analyzed by a two-way ANOVA. Biodentine showed a significantly higher mean push-out bond strength value than DiaRoot-BioAggregate (P=0.00). The medications have an effect on the push-out bond strength of both materials (P=0.002). Biodentine showed better adhesive performance as an apical plug than DiaRoot-BioAggregate.Öğe The effect of Nd:YAG laser irradiation on root surface: shear-bond strength and SEM-EDX study(Taylor & Francis Ltd, 2015) Akman, Melek; Celik, Kezban; Ozcopur, Betul; Terlemez, ArslanTo investigate the effects of Nd:YAG laser and 17% EDTA treatment on root-dentin mineral content using scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDX) and on shear-bond strength of epoxy-resin-based sealer (AH Plus) to root dentin. Twelve extracted premolars were decoronated and roots were sectioned, so that 24 two-root halves were obtained. Element levels of each half were examined by SEM/EDX, and AH Plus build-ups were created. After shear-testing, the test surfaces were reground and subjected to a 5.25% NaOCl. Two subgroups were created according to the surface treatment (n=12): G1, with 17% EDTA for 5min; G2, with the Nd:YAG laser. The element level analysis and shear-bond strength test were repeated for each half; the data were recorded (MPa) and analyzed (paired samples t-test). The EDTA treatment increased the O, C, Ca/P ratio (p<0.001), decreased Ca, P content (p=0.000), but did not change Na, Mg content (p>0.05). The Nd:YAG laser increased O, Ca/P ratio (p<0.001), and decreased the Ca, P content (p=0.000). The C, Mg, Na content did not change with the Nd:YAG laser (p>0.05). Both 17% EDTA and Nd:YAG laser had an effect on the mineral content of roots. The 17% EDTA treatment decreased the shear-bond strength of AH Plus to root dentin (p=0.000); however, the Nd:YAG laser did not affect the bond strength (p=0.238). Thus, an Nd:YAG laser can be used for disinfection of the root canal when AH Plus is used as a sealer.Öğe Efficacy of Twisted File Adaptive, Reciproc and ProTaper Universal Retreatment instruments for root-canal-filling removal: A cone-beam computed tomography study(Japanese Soc Dental Materials Devices, 2016) Akbulut, Makbule Bilge; Akman, Melek; Terlemez, Arslan; Magat, Guldane; Sener, Sevgi; Shetty, HeereshThe aim of this study was to evaluate the efficacy of Twisted File (TF) Adaptive, Reciproc, and ProTaper Universal Retreatment (UR) System instruments for removing root-canal-filling. Sixty single rooted teeth were decoronated, instrumented and obturated. Preoperative CBCT scans were taken and the teeth were retreated with TF Adaptive, Reciproc, ProTaper UR, or hand files (n=15). Then, the teeth were rescanned, and the percentage volume of the residual root-canal-filling material was established. The total time for retreatment was recorded, and the data was statistically analyzed. The statistical ranking of the residual filling material volume was as follows: hand file=TF Adaptive>ProTaper UR=Reciproc. The ProTaper UR and Reciproc systems required shorter periods of time for retreatment. Root canal filling was more efficiently removed by using Reciproc and ProTaper UR instruments than TF Adaptive instruments and hand files. The TF Adaptive system was advantageous over hand files with regard to operating time.Öğe Fracture resistance of roots obturated with a novel calcium silicate-based endodontic sealer (BioRoot RCS)(Taylor & Francis Ltd, 2016) Guneser, Mehmet Burak; Akman, Melek; Kolcu, Inci Baser; Eldeniz, Ayce UnverdiThe aim of this ex vivo study was to evaluate the vertical-fracture resistance of roots obturated with a newly developed tricalcium silicate cement (BioRoot RCS; Septodont, Saint Maur Des Fosses, France) using cold lateral compaction technique (LC) or matched-taper single-cone gutta-percha technique (SC). Decoronated 82 single-rooted mandibular premolars were chemo-mechanically prepared and then randomly divided into 6 experimental groups (n = 12) and 2 control groups (n = 5): Group 1 iRoot SP-LC; Group 2 iRoot SP-SC; Group 3 MTA Fillapex-LC; Group 4 MTA Fillapex-SC; Group 5 BioRoot RCS-LC and Group 6 BioRoot RCS-SC. In the positive-control group, roots were instrumented but not filled, and in the negative-control group, roots were neither instrumented nor filled. All samples were incubated for two weeks and then subjected to vertical loading force (1 mm/min) until fracture. The force required to fracture each specimen were determined, and the data were statistically analyzed. The highest significant fracture resistance was recorded for the iRoot SP-LC, iRoot SP-SC, BioRoot RCS-LC, and BioRoot RCS-SC groups, with no significant difference among them (p > 0.05) when compared with the positive-control group (p < 0.05), whereas the lowest significant values were observed in the MTA Fillapex-LC and MTA Fillapex-SC groups (p < 0.05). The vertical fracture resistance of roots obturated with BioRoot RCS and iRoot SP sealers using either LC or SC technique was found to be similar to that of intact teeth. BioRoot RCS, newly developed tricalcium silicate cement, might have the potential to reinforce the instrumented teeth against vertical root fracture.Öğe Perforasyon tamir materyallerinin sızıntı ve kalitesi: İntrakoronal ve retrograd tekniklerin karşılaştırılması(2014) Özçopur, Betül; Akman, Melek; Hakkı, Sema; Belli, SemaAmaç: Bu in vitro çalışmanın amacı, perforasyon tamir materyallerinin, perforasyon bölgesine ortograd ya da retrograd yerleştirildiğinde, sızıntı ve kalitesini değerlendirmektir. Gereç ve Yöntemler: Çekilmiş insan molar dişlerin mesial ve distal kök yüzeylerinde, dişin uzun aksına 45 derecelik açıyla elmas bir frez ile kasten perforasyonlar oluşturuldu. Mesial perforasyonlar intrakoronal olarak şu materyaller kullanılarak tamir edildi: IRM (Dentsply), amalgam (Dentsply), Dyract (Dentsply), SuperBond C&B (Sun Medical) and MTA (Dentsply). Siman ile giriş kavitesi doldurulduktan sonra distal perforasyonlar aynı materyaller ile retrograd olarak tamir edildi. Dişler %100 nemli ortamda 24 saat saklandı. Perforasyon bölgeleri 24 saat %2'lik metilen mavisinde bekletildi. Dişler kesilerek, 20x ve 40x büyütme ile stereomikroskop altında incelendi ve boya penetrasyonuna göre taşmış, yeterli ve yetersiz olarak skorlandı. Bulgular: Veriler Kruskal-Wallis ve MannWhitney U-testleri ile analiz edildi. Restorasyon teknikleri arasında önemli derecede fark bulundu (p0.05). Tüm materyallerde retrograd teknik kullanıldığı zaman daha az sızıntı gözlendi (p0.05). Giriş kavitesi boyunca perforasyonun tamiri, %86 taşmış ya da yetersiz bulundu. Retrograd olarak uygulandığı zaman IRM %80, MTA %60 oranında sızıntı olmadan yeterli bulundu. Sonuç: Retrograd teknik kullanarak perforasyonların tamiri, kullanılan materyalin etkisi olmaksızın yeterli restorasyonun sayısı önemli derecede arttı. IRM retrograd uygulandığı zaman MTA'yı takiben en iyi kapamayı sağladı.Öğe Push-out bond strength of a new post system after various post space treatments(Japanese Soc Dental Materials Devices, 2016) Akman, Melek; Eldeniz, Ayce Unverdi; Ince, Selen; Guneser, Mehmet BurakTo assess the effect of post-space treatment with chelating agents on the push-out bond-strength of a glass fiber post-system. Fortyeight human teeth were decoronated. The roots were prepared to size 40 and obturated. The post-spaces were prepared with PeesoReamer drills. The post-spaces were irrigated with (i) NaOCl and EDTA with chlorhexidine (QMix), NaOCl and EDTA, NaOCl and Citric acid, (iv) NaOCl and saline as a control group. i-TFC glass fiber posts were then luted with i-TFC bond system. The samples were horizontally sectioned. The displacement resistance was measured. Push-out bond-strength (MPa) was calculated. Data were analyzed. NaOCl/QMix group showed highest values to dentine whereas NaOCl/Citric acid group showed lowest values. i-TFC post-system demonstrated equal bond strength values when post-space treated with either NaOCl/EDTA or NaOCl/Saline. It can be concluded that post-space could be treated with NaOCl and QMix in order to increase adhesion of i-TFC post-system to root dentine.Öğe Tooth discoloration effects of calcium silicate based barrier materials used in revascularization and treatment with internal bleaching(Elsevier Taiwan, 2017) Akbulut, Makbule Bilge; Terlemez, Arslan; Akman, Melek; Buyukerkmen, Begum; Guneser, Mehmet Burak; Eldeniz, Ayce UnverdiBackground/purpose: Usage of barrier materials is an important step in revascularization procedure. One of the undesired properties of these barrier materials is to cause coronal tooth discoloration. The aim of this in vitro study was to evaluate the tooth discoloration induced by ProRoot MTA (PMTA), Biodentine, and MM-MTA, as well as the efficacy of internal bleaching on this discoloration. Materials and methods: Forty-two maxillary incisor teeth were prepared. Triple antibiotic paste (TAP) was placed in the root canals and incubated for 3 weeks. After removing the TAP, blood embedded spongostans were inserted into the root canals, and PMTA, Biodentine, or MM-MTA was placed over them. The teeth were incubated for 4 weeks at 37 degrees C; then, the internal bleaching agent was sealed for one week. The tooth color was measured throughout the study and the color change values (Delta E) of each specimen were calculated, and the data was statistically analyzed using the one-way ANOVA and Tamhane's T2 tests. Results: The TAP significantly decreased the luminosity of the teeth (p < 0.05); however, no significant differences were observed between the tooth discolorations induced by the PMTA, Biodentine, and MM-MTA (p > 0.05). The teeth in the Biodentine group were more whitened than those of the PMTA and MM-MTA groups (p < 0.05). Conclusion: Although the PMTA, Biodentine, and MM-MTA caused similar color alterations in the teeth, more bleaching was observed on those teeth discolored using TAP + blood + Biodentine. (C) 2017 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.Öğe Which is effective on bond strength of resin-based sealers: incorporation of powdered dentin to primer or adhesive?(Taylor & Francis Ltd, 2015) Akman, Melek; Belli, Sema; Ozcopur, Betul; Eskitascioglu, GuercanThe aim of this study was to investigate the effect of adding powdered dentin to primer or adhesive in a self-etch system on the shear bond strength of three resin-based sealers. Seventy-two premolars were sectioned buccolingually, and 144 root halves were divided into three groups according to the sealer used: epoxy resin-based sealer (AH Plus), methacrylate resin-based (RealSeal, Hybrid Root SEAL) n=48. The surfaces were irrigated with 5% NaOCl, 17% EDTA, distilled water for 5min. Four subgroups were created (n=12): control group; Clearfil Liner Bond 2V treated group; powdered dentin added to the primer of Clearfil Liner Bond 2V (40 wt.%); and powdered dentin added to the adhesive of Clearfil Liner Bond 2V (20 wt.%). Dentin powder was prepared. Three mm high buildups with a constant surface area of 3.45mm(2) were created using the sealers and allowed to set (37oC, 100% humid, 72h). The samples were tested to failure for shear bond strength (1mm/min). The data were calculated (MPa) and analyzed using two-way ANOVA, one-way ANOVA, and Tukey HSD tests. Adhesive use decreased the bonding performance of AH Plus (p=0.00). Mean bond strength of the other sealers was found similar to control. Primer or adhesive resin with powdered dentin did not increase the adhesive performance of the self-etch system used. The shear bond strength of RealSeal was significantly increased when powdered dentin was added to primer or adhesive (p=0.00). The effect of adding powdered-dentine to primer or adhesive in a self-etch system on the shear bond strength was sealer-dependent .