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Öğe Atopy and allergic diseases in immunoglobulin A deficiency(Bilimsel Tip Yayinevi, 2012) Artac, Hasibe; Keles, Sevgi; Karagol, Cuneyt; Gokturk, Bahar; Reisli, IsmailObjective: Immunoglobulin (Ig) A deficiency is a common immune system disorder which causes morbidity especially in preschool children. The aim of this study is to evaluate atopy and allergic diseases of cases with IgA deficiency, determinate clinical results before and after four years of age. Materials and Methods: The data records of 295 children with IgA deficiency (272 partial, 23 selective IgA deficiency) between November 2001 and 2007 were examined retrospectively. Results: The mean age at the diagnosis was 64.3 +/- 43 (4-204) months, the mean age at the beginning of infection was 29.5 +/- 36 (1-168) months. There were 121 patients under the age of four years at the diagnosis time. The clinical presentations were recurrent bronchitis (40%), tonsillitis/pharyngitis (33%), sinusitis (6%), pneumonia (4%), acute otitis media (2%), acute gastroenteritis (0.3%) and neck abscess (0.3%), respectively. Eosinophilia was present in 25% of patients, increased IgE levels was present in 38% of patients, and they were correlated with each other. Atopy was detected in 31% of patients by spesific IgE and/or skinprick test positivity. Allergic disease prevalance was found as % 78 (60% asthma, 15% allergic rhinitis, 8% atopic dermatitis, 6% urticaria, 0.7% allergic conjunctivitis). During the clinical follow-up IgA levels reached to normal levels at 17 +/- 14 months ranged between 3 and 60 months. Three patients with selective IgA deficiency had bronchiectasia. Conclusion: We found that allergic diseases were increased in these patients. Serum IgA level should be tested not only for recurrent infections but also for patients with allergic symptoms.Öğe B cell aplasia and hypogammaglobulinemia associated with levetiracetam(K Faisal Spec Hosp Res Centre, 2018) Ozdemir, Hulya; Sumer, Sua; Karabagli, Hakan; Akdemir, Gokhan; Caliskaner, A. Zafer; Artac, HasibeLevetiracetam (LEV) is a second-generation antiepileptic drug approved for the treatment of several types of epilepsy. We report a 45-year-old female who developed hypogammaglobulinemia and B cell aplasia during LEV treatment. The Naranjo probability score for an adverse drug reaction was 6. After LEV discontinuation, the number of B cells gradually increased and reached normal levels within two months. This case suggests that monitoring of immunoglobulin levels and lymphocyte subsets analysis is important in patients treated with LEV, especially in cases of prolonged infections.Öğe A CASE WITH ABSENCE OF B LYMPHOCYTES(Springer/Plenum Publishers, 2014) Ozdemir, Hulya; Artac, Hasibe; Ural, Onur; Karabagli, Hakan; Caliskaner, A. Zafer[Abstract Not Availabe]Öğe The change of HLA DR expressions and monocyte subsets in non-small cell lung cancer(Wiley, 2021) Emsen, Ayca; Artac, Mehmet; Artac, Hasibe[Abstract Not Availabe]Öğe The effects of trastuzumab, paclitaxel, and carboplatin on HER2-positive cancer stem cells that are isolated from primary breast cancer cultures: a preliminary report(Amer Assoc Cancer Research, 2015) Artac, Mehmet; Kayadibi, Gozde; Ceylan, Ayca; Kars, Meltem Demirel; Artac, Hasibe; Cakir, Murat; Boruban, Cem[Abstract Not Availabe]Öğe Efficacy of intravenous immunoglobulin treatment in immunocompromised children with H1N1 influenza: a clinical observation(Wiley-Blackwell, 2016) Gokturk, Bahar; Pekcan, Sevgi; Guner, Sukru Nail; Artac, Hasibe; Keles, Sevgi; Kirac, Mine; Reisli, IsmailBackground and AimsThe appropriate treatment of pandemic H1N1 influenza which was first identified in April 2009 in Mexico is insufficient especially for immunocompromised patients. We aimed to evaluate the features and prognostic factors of the children with H1N1, especially immunocompromised ones, and whether intravenous immunoglobulin G (IVIG) replacement could aid for a better outcome. MethodsTwenty-one hospitalized children with laboratory-confirmed H1N1 were evaluated retrospectively. Data were extracted from files and electronic medical records. ResultsThe median age was 37 (1-216) months; 62% of them were under 5years of age and 71.4% had one or more underlying disorders. Main symptoms were high fever, cough, fatigue and vomiting. Lower respiratory tract manifestations were seen in 66.6% of children. Mortality rate was 4.7%. The patient who died had the lowest lymphocyte (100/mm(3)), thrombocyte (21000/mm(3)) and highest blood urea nitrogen (87mg/dL) levels. Fifty-eight percent of evaluated patients had one of the primary immunodeficiency disorders. Surprisingly, none of the six patients with primary immunodeficiency who are on regular IVIG replacement needed intensive care unit and died. Although median durations of cough, fever and hospitalization were lower, they did not change statistically according to get IVIG replacement regularly (P=0.47, 0.97, 0.09, respectively). ConclusionOur study is important while it is the first one that shows the course of primary immunodeficient children with H1N1 infection who were on regular IVIG replacement. A trial of high-dose IVIG may be a useful adjunctive therapy in severe H1N1 influenza, particularly in the immunocompromised patients.Öğe Increased PD-1 and EGFR expression levels of T lymphocytes in patients with non-small cell lung cancer.(Lippincott Williams & Wilkins, 2021) Ceylan, Ayca; Artac, Mehmet; Artac, Hasibe[Abstract Not Availabe]Öğe Increased PD-1 and EGFR expression levels of T lymphocytes in patients with non-small cell lung cancer.(Lippincott Williams & Wilkins, 2021) Ceylan, Ayca; Artac, Mehmet; Artac, Hasibe[Abstract Not Availabe]Öğe A Novel Mutation in the Complement Component 3 Gene in a Patient with Selective IgA Deficiency(Springer/Plenum Publishers, 2013) Santos-Valente, Elisangela; Reisli, Ismail; Artac, Hasibe; Ott, Raphael; Sanal, Ozden; Boztug, KaanPurpose Immunological and molecular evaluation of a patient presenting with recurrent infections caused by Streptococcus pneumoniae and low complement component 3 (C3) levels. Methods Immunological evaluation included complement components and immunoglobulin level quantification as well as number and function of T cells, B cells and neutrophils. Serotype-specific immunoglobulin G antibodies against S. pneumoniae capsular polysaccharides were quantified by ELISA in serum samples before and after vaccination with unconjugated polysaccharide vaccine. For the molecular analysis, genomic DNA from the patient and parents were isolated and all exons as well as exon-intron boundaries of the C3 gene were sequenced by Sanger sequencing. Results A 16-year-old male, born to consanguineous parents, presented with recurrent episodes of pneumonia caused by S. pneumoniae and bronchiectasis. The patient showed severely reduced C3 and immunoglobulin A levels, while the parents showed moderately reduced levels of C3. Mutational analysis revealed a novel, homozygous missense mutation in the C3 gene (c. C4554G, p. Cys1518Trp), substituting a highly conserved amino acid in the C345C domain of C3 and interrupting one of its disulfide bonds. Both parents were found to be carriers of the affected allele. Vaccination against S. pneumoniae resulted in considerable clinical improvement. Conclusions We report a novel homozygous mutation in the C3 gene in a patient with concomitant selective IgA deficiency who presented with a marked clinical improvement after vaccination against S. pneumoniae. This observation underlines the notion that vaccination against this microorganism is an important strategy for treatment of PID patients, particularly those presenting with increased susceptibility to infections caused by this agent.Öğe Protein functionality as a potential bottleneck for somatic revertant variants(Mosby-Elsevier, 2021) Kaiser, Fabian M. P.; Reisli, Ismail; Pico-Knijnenburg, Ingrid; Langerak, Anton W.; Kavelaars, Francois G.; Artac, Hasibe; IJspeert, Hanna[Abstract Not Availabe]Öğe Reduced CD19 expression and decreased memory B cell numbers in transient hypogammaglobulinemia of infancy(Springer-Verlag Italia Srl, 2013) Artac, Hasibe; Kara, Reyhan; Gokturk, Bahar; Reisli, IsmailWe aimed to evaluate the role of the CD19 complex in the pathogenesis of transient hypogammaglobulinemia of infancy (THI) and to better characterize the subsets of memory B cells. The study population consisted of 22 male and 14 female patients with a mean age at presentation of 20 +/- A 9.9 months. The CD19 complex and B cell subsets were evaluated by flow cytometry. While the CD19 median fluorescence index (MFI) in patients with THI was significantly lower than controls (122.9 +/- A 66.7 in patients; 184.2 +/- A 39 in controls, p < 0.01), expression of CD21 and CD81 was increased (94.4 +/- A 3, 96.8 +/- A 2.5 % in patients; 91 +/- A 3.9; 94.7 +/- A 3.5 % in controls, p < 0.01 vs. p < 0.05, respectively). The expressions of switched memory B cells and IgM memory B cells were found to be reduced in THI. Considering that the CD19 complex regulates the events following antigen stimulation, the change in CD19 complex detected in THI may be related to insufficiency of antibody production.Öğe Reduced Monocyte Subsets, Their HLA-DR Expressions, and Relations to Acute Phase Reactants in Severe COVID-19 Cases(Mary Ann Liebert, Inc, 2022) Cizmecioglu, Ahmet; Emsen, Ayca; Sumer, Sua; Ergun, Dilek; Akay Cizmecioglu, Hilal; Turk Dagi, Hatice; Artac, HasibeMonocytes are one of the principal immune defense cells that encounter infectious agents. However, an essential role of monocytes has been shown in the spread of viruses throughout the human body. Considering this dilemma, this study aimed to evaluate monocyte subsets and Human Leukocyte Antigen-DR isotype (HLA-DR) expressions in clinical coronavirus disease 2019 (COVID-19) cases. This prospective, multicenter, case-control study was conducted with COVID-19 patients and healthy controls. The patient group was divided into two subgroups according to disease severity (severe and non-severe). Three monocyte subsets (classical, CL; intermediate, INT; non-classical, NC) were analyzed with flow cytometry upon the patients' hospital admission. A total of 42 patients with COVID-19 and 30 controls participated in this study. The patients' conditions were either severe (n = 23) or non-severe (n = 19). All patients' monocyte and HLA-DR expressions were decreased compared with the controls (p < 0.05). Per disease severity, all monocyte subsets were not significant with disease severity; however, the HLA-DR expressions of CL monocytes (p = 0.002) and INT monocytes (p = 0.025) were more decreased in the severe patient group. In patients with various clinical features, NC monocytes were more affected. Based on these results, NC monocytes were more decreased in acute COVID-19 cases, though related various clinics decreased all monocyte subsets in these patients. Decreased monocyte HLA expressions may be a sign of immune suppression in severe patients, even when the percentage of monocyte levels has not decreased yet.Öğe Response to trastuzumab and investigation of expression profiles of matrix metalloproteinase-related proteins in primary breast cancer stem cells(Springer-Verlag Italia Srl, 2021) Koygun, Gozde Kayadibi; Kars, Meltem Demirel; Emsen, Ayca; Artac, Hasibe; Aksoy, Faruk; Cakir, Murat; Tavli, LemaBreast cancer (BC) is the leading cause of cancer deaths in women. One of the reasons for the failure of BC treatment is reportedly the ineffectiveness of chemotherapeutic drugs against breast cancer stem-like cells (BCSCs). HER2 receptors have an important role in the self-renewal of BCSCs. Matrix metalloproteinase (MMP) and cytokine levels were found to be higher in BCSCs, which demonstrates their potential metastatic capacity. Therefore, the aim of this study was to evaluate the response of BCSCs to trastuzumab and to investigate the MMP levels in primary breast cancer cells and HER2(+) BCSCs. Tumour tissue samples were obtained during surgical intervention from ten breast cancer patients, and primary culture cells were established from these tissues. Four major molecular subgroups were sorted from the primary culture: HER2(+) BCSCs (CD44(+)CD24(-)HER2(+)), HER2(-) BCSCs (CD44(+)CD24(-)HER2(-)), HER2(-) primary culture cells (CD44(+)CD24(+)HER2(-)) and triple positive primary culture cells (CD44(+)CD24(+)HER2(+)). These cells were cultured and treated with trastuzumab, paclitaxel, carboplatin, and the combination of those three drugs for 96 h. Cellular responses to these drugs were determined by XTT cytotoxicity test. MMPs and cytokine array analysis showed that MMPs and TIMP-1, TIMP-2 proteins were expressed more in HER2(+) BCSCs than in primary culture. HER2(-) BCSCs were more resistant to drugs than HER2(+) BCSCs. Our findings suggest that the presence of HER2(-) BCSCs may be responsible for primary trastuzumab resistance in HER2(+) BC cell population. Further studies investigating the function of MMPs are needed for drug targeting of BCSCs.Öğe The role of regulatory T cells in allergic rhinitis and their correlation with IL-10, IL-17 and neopterin levels in serum and nasal lavage fluid(Springer, 2020) Erkan, Kadriye; Bozkurt, Mete K.; Artac, Hasibe; Ozdemir, Hulya; Unlu, Ali; Korucu, Emine N.; Elsurer, CagdasPurpose Allergic rhinitis (AR), is an IgE-mediated inflammation of the nose. Regulatory T cells (Tregs) and inflammatory cytokines have been shown to play a critical role in allergic airway inflammation. The aim of the study was to compare the levels of blood T lymphocyte subsets and IL-10, IL-17 and neopterin concentrations in serum and nasal lavage of patients with AR compared to healthy subjects. Methods The study included 38 subjects with moderate-severe AR and 36 sex- and age-matched controls. Peripheral blood CD3+, CD3+CD4+ and CD4+CD25+Foxp3 percentages were evaluated using flow cytometry. Levels of IL-10, IL-17 and neopterin were measured both in serum and nasal lavage fluid with ELISA and HPLC, respectively. Results No difference was found in the percentages of T lymphocyte subsets between the two groups (p > 0.05). Serum IL-10 levels were similar (p > 0.05), whereas nasal IL-10 was lower in AR subjects compared to control group (2.22 +/- 0.91 and 3.12 +/- 1.45 pg/ml, respectively) (p < 0.05). Mean serum and nasal IL-17 were higher in AR (107.7 +/- 79.61 and 527.36 +/- 738.7 pg/ml) than the control group (76.29 +/- 28.94 and 328.9 +/- 430.8 pg/ml) (p < 0.05 and p > 0.05). There were no significant differences in serum and nasal neopterin levels (p > 0.05). Conclusions Although there were no differences in the distribution of lymphocyte subsets between the AR and control groups, the finding of higher levels of serum and nasal IL-17 and lower levels of nasal IL-10 support the cytokine imbalance in the pathogenesis of AR.Öğe Systemic Atopy and Immunoglobulin Deficiency in Turkish Patients with Vernal Keratoconjunctivitis(Taylor & Francis Inc, 2013) Bozkurt, Banu; Artac, Hasibe; Arslan, Nasha; Gokturk, Bahar; Bozkurt, Mete Kaan; Reisli, Ismail; Irkec, MuratPurpose: To determine the prevalence of systemic atopy and immunoglobulin (Ig) deficiencies in vernal keratoconjunctivitis (VKC). Methods: Sixty-seven VKC subjects (79.1% boys) with a mean age of 11.3 +/- 4.3 years were included. Serum Ig levels and specific IgE levels were measured using the nephelometric method and reversed enzyme immunoassay with sandwich ELISA technique, respectively. The patients underwent epidermal skin tests with commercial extracts. Results: Family history of atopy and associated systemic allergies were detected in 32.8 and 40.3% of the subjects, respectively. Blood eosinophilia, elevated total, and specific IgE and positive skin tests were detected in 33.8, 42.2, 50, and 35% of the subjects, respectively. Out of 62 subjects, low levels of IgA, IgG, IgM, and IgG3 were detected in 12.9, 8, 6.5, and 1.6% of the patients, respectively. Conclusion: IgE-mediated mechanisms are involved in approximately 40% of VKC patients. A new finding was the higher incidence of Ig deficiency.Öğe THE VALUE OF MEAN PLATELET VOLUME/PLATELET COUNT RATIO TO PREDICT 22q11.2 DELETION SYNDROME(Springer/Plenum Publishers, 2014) Gokturk, Bahar; Guner, Sukru Nail; Kara, Reyhan; Kirac, Mine; Keles, Sevgi; Artac, Hasibe; Reisli, Ismail[Abstract Not Availabe]Öğe VEGFR2 expressions in Th1 and CD8+cytotoxic T lymphocytes (CTL) in colon cancer patients.(Lippincott Williams & Wilkins, 2021) Artac, Mehmet; Ceylan, Ayca; Eryilmaz, Melek Karakurt; Araz, Murat; Karaagac, Mustafa; Artac, Hasibe; Emiroglu, Melike[Abstract Not Availabe]Öğe VEGFR2 expressions in Th1 and CD8+cytotoxic T lymphocytes (CTL) in colon cancer patients.(Lippincott Williams & Wilkins, 2021) Artac, Mehmet; Ceylan, Ayca; Eryilmaz, Melek Karakurt; Araz, Murat; Karaagac, Mustafa; Artac, Hasibe; Emiroglu, Melike[Abstract Not Availabe]Öğe Would mean platelet volume/platelet count ratio be used as a novel formula to predict 22q11.2 deletion syndrome?(Allergy Immunol Soc Thailand, 2016) Gokturk, Bahar; Guner, Sukru Nail; Kara, Reyhan; Kirac, Mine; Keles, Sevgi; Artac, Hasibe; Zamani, Ayse GulBackground: The diagnosis of 22q11.2 deletion syndrome depends on a time-consuming and expensive method, fluorescence in situ hybridisation (FISH). Objectives: We aimed to determine new parameters which can aid for in the diagnosis of 22q11.2 deletion syndrome. Methods: Twenty two patients with 22q11.2 or 10p13 deletion were evaluated retrospectively. Results: Facial-dysmorphism and mental-motor retardation were detected in 100% of patients. Mean platelet (PLT) counts were lower (224,980 versus 354,000, p = 0.001), mean PLT volume (MPV) (9.95 versus 7.07, p = 0.002), and MPV/PLTx10(5) ratios (5.36 versus 2.08, p < 0.001) were higher in patients with 22q11.2 deletion compared with the control group. Area under the receiver-operator characteristic (ROC) curve was 0.864, sensitivity was 84.6%, specificity was 90.9%, positive predictive value (PPV) was 91.7%, and negative predictive value (NPV) was 83.3% when MPV was 8.6. Area under ROC curve was 0.864, sensitivity was 76.9%, specificity was 90.1%, PPV was 90.1%, and NPV was 76.3% when PLT was 265,500. Area under ROC curve was 0.906, sensitivity was 84.6%, specificity was 100%, PPV was 100%, and NPV was 84.6% when MPV/PLTx10(5) was 3.3. Expression of PLT surface markers which were not in the GPIb-V-IX receptor complex (CD61, CD41a) increased as the surface area increased, but markers which were in a complex (CD42a, CD42b) did not change. Conclusions: High MPV/PLT value can be a good predictor for the diagnosis of 22q11.2 deletion syndrome. We suggest that in patients with facial dysmorphism and retardation in neurodevelopmental milestones and if MPV >= 8.6fl, MPV/PLTx10(5) ratio >= 3.3 and PLT count <= 265,500/mm(3), the patients should be tested by FISH analysis to confirm the 22q11.2 deletion. If there are no macrothrombocytes, the 10p13 deletion should be tested in suspected cases.Öğe Would mean platelet volume/platelet count ratio be used as a novel formula to predict 22q11.2 deletion syndrome?(Allergy Immunol Soc Thailand, 2016) Gokturk, Bahar; Guner, Sukru Nail; Kara, Reyhan; Kirac, Mine; Keles, Sevgi; Artac, Hasibe; Zamani, Ayse GulBackground: The diagnosis of 22q11.2 deletion syndrome depends on a time-consuming and expensive method, fluorescence in situ hybridisation (FISH). Objectives: We aimed to determine new parameters which can aid for in the diagnosis of 22q11.2 deletion syndrome. Methods: Twenty two patients with 22q11.2 or 10p13 deletion were evaluated retrospectively. Results: Facial-dysmorphism and mental-motor retardation were detected in 100% of patients. Mean platelet (PLT) counts were lower (224,980 versus 354,000, p = 0.001), mean PLT volume (MPV) (9.95 versus 7.07, p = 0.002), and MPV/PLTx10(5) ratios (5.36 versus 2.08, p < 0.001) were higher in patients with 22q11.2 deletion compared with the control group. Area under the receiver-operator characteristic (ROC) curve was 0.864, sensitivity was 84.6%, specificity was 90.9%, positive predictive value (PPV) was 91.7%, and negative predictive value (NPV) was 83.3% when MPV was 8.6. Area under ROC curve was 0.864, sensitivity was 76.9%, specificity was 90.1%, PPV was 90.1%, and NPV was 76.3% when PLT was 265,500. Area under ROC curve was 0.906, sensitivity was 84.6%, specificity was 100%, PPV was 100%, and NPV was 84.6% when MPV/PLTx10(5) was 3.3. Expression of PLT surface markers which were not in the GPIb-V-IX receptor complex (CD61, CD41a) increased as the surface area increased, but markers which were in a complex (CD42a, CD42b) did not change. Conclusions: High MPV/PLT value can be a good predictor for the diagnosis of 22q11.2 deletion syndrome. We suggest that in patients with facial dysmorphism and retardation in neurodevelopmental milestones and if MPV >= 8.6fl, MPV/PLTx10(5) ratio >= 3.3 and PLT count <= 265,500/mm(3), the patients should be tested by FISH analysis to confirm the 22q11.2 deletion. If there are no macrothrombocytes, the 10p13 deletion should be tested in suspected cases.
