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Öğe 3?,4?-Dihydroxyflavonol (DiOHF) prevents DNA damage, lipid peroxidation and inflammation in ovarian ischaemia-reperfusion injury of rats(Taylor & Francis Inc, 2022) Agartan, Elif Sena; Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim; Menevse, Esma; Dasdelen, Dervis; Avunduk, Mustafa CihatThis study aimed to determine the effect of 3',4'-Dihydroxyflavonol (DiOHF) on lipid peroxidation, DNA damage and inflammation in ovarian ischaemia (I)-reperfusion (R) injury. This study was performed on 44 Wistar-albino female rats. Groups were designed as Control; Sham; I/R (the left ovary was ligated for 2 h and then reperfused for 2 h); I/R + DiOHF (after 2 h ischaemia and 2 h reperfusion, 30 mg/kg of DiOHF was given intraperitoneally and reperfusion was allowed for 2 h more); I + DiOHF + R (after 2 h I, 30 mg/kg of DiOHF was given at the beginning of 2 h reperfusion); DiOHF + I/R (2 h after DiOHF administration, the left ovary was ligated for 2 h and then reperfused for 2 h). Blood and ovarian tissue samples were analysed for GSH, MDA, 8-OHdG, SOD, and IL-6. Ovarian tissue was examined histopathologically. Ovarian I/R has led to inflammation and oxidative damage. However, DiOHF activated the antioxidant system and prevented DNA damage induced by I/R in ovarian tissue. Vascularisation, oedema, and inflammation also occurred in ovarian tissue in I/R group. The results of this study indicated that I/R led to disturbance of the oxidant/antioxidant system balance and increased DNA damage; however, DiOHF supplementation prevented DNA damage, lipid peroxidation and inflammation by increasing the antioxidant system in ovarian I/R injury in rats. However, in potential I/R situations, DiOHF application appears to be beneficial in reducing inflammation, oxidant injury, and DNA damage, and in activating the antioxidant system. IMPACT STATEMENT What is already known on this subject? Ischaemia/reperfusion (I/R) injuries lead to damage in cells or tissues due to insufficient blood flow. What do the results of this study add? Increased DNA injury and inflammatory response (IL-6) and structural impairment were treated by administration of intraperitoneal (DiOHF) which strongly stimulated the antioxidant system, inhibited antioxidant activities, prevented DNA damage and inflammation process. What are the implications of these findings for clinical practice and/or further research? This study's strength is that it is the first research demonstrates the prevention of DNA damage in ovarian I/R by DiOHF supplementation. This flavonoid (DiOHF) may be used for treatment in different ovarian ischaemia/reperfusion.Öğe 3?,4?-Dihydroxyflavonol attenuates spatial learning and memory impairments in global cerebral ischemia(Taylor & Francis Ltd, 2017) Oz, Mehmet; Demir, Enver Ahmet; Caliskan, Merve; Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim; Atalik, K. Esra NurullahogluObjectives: In the present study, effects of 3', 4'-dihydroxyflavonol (DiOHF) on anxiety-like behavior, and learning and memory were investigated in a model of transient global cerebral ischemia and reperfusion. Methods: The animals were assigned to sham-operated, ischemia, and two DiOHF-treated (10 mg/kg i. p.) groups. DiOHF was administered at 1 hour before and immediately after the ischemia. Male rats were subjected to bilateral common carotid artery occlusion to induce acute cerebral ischemia for 20 minutes, followed by reperfusion for 7 days. The openfield, elevated plus maze (EPM), and Morris water maze tests were used to evaluate the effects of DiOHF treatment on ischemia-induced locomotor activity, anxiety-like behavior, and spatial and recognition memory impairments, respectively. Results: In the open field test, locomotor activity in the ischemic rats was not altered 6 days after the ischemia, nor was anxiety-like behavior, which was evaluated with the EPM (P > 0.05). In the water-maze test, cerebral ischemia significantly decreased the exploration time in the target quadrant, and the platform crossing counts were lower (P < 0.05) in the probe trial test; this memory impairment was significantly improved by DiOHF applied 1 hour before and immediately after ischemia (P < 0.05). Discussion: All together, these findings suggest that DiOHF reverses spatial learning and memory deficits resulting from transient global ischemia but has no significant effect on anxiety-like behavior.Öğe Changes in the Serum Levels of Trace Elements Before and After the Operation in Thyroid Cancer Patients(Humana Press Inc, 2017) Baltaci, Abdulkerim Kasim; Dundar, Tugrul Kadir; Aksoy, Faruk; Mogulkoc, RasimThe present study aims to examine the changes in the serum levels of trace elements before and after the operation in thyroid cancer patients. The study registered 50 individuals, of whom 25 were female and 25 were male. The patients were allocated to four groups: group 1: male thyroid cancer patients group (n = 15), group 2: female thyroid cancer patients group (n = 15), group 3: male control group (n = 10), group 4: female control group (n = 10). The subjects in groups 1 and 2 were the patients who were post-operatively diagnosed with a pathological malignancy in the thyroid tissue samples. Blood samples were collected from all subjects before the operation, immediately after the operation, and on the post-operative day 15. Additionally, thyroid tissue samples were taken from all subjects post-operatively. Some elements in the blood and tissue samples were determined using the atomic emission method. Zinc and selenium levels of groups 1 and 2 in the pre- and post-operative measurements were significantly lower than those in the control groups (p < 0.05), but were higher in the thyroid tissue (p < 0.05). Serum zinc and selenium levels measured in the subjects on the post-operative day 15 were similar to those measured in the controls. Our study show that changes in the serum and thyroid tissue levels of trace elements like zinc and selenium, which play a critical role in thyroid function, might be associated with the pathogenesis of thyroid cancer.Öğe Determination of Effective Mechanism of Melatonin in Hyperthermic Febrile Convulsion in Rats(Karger, 2018) Aydin, Leyla; Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim[Abstract Not Availabe]Öğe Effect of 3?-4?-dihydroxyflavonol on lipid per oxidation in experimental renal ischemia-reperfusion in rats(Univ Karachi, 2019) Koc, Aynur; Ergene, Neyhan; Baltaci, Abdulkerim Kasim; Mogulkoc, RasimThis study aimed to examine the affects of 3'-4'-dihydroxyflavonol (DiOHF) on lipid peroxidation in experimental renal ischemia-reperfusion. The research was conducted on Wistar-albino type male rat. The experimental groups were formed as 1.Control; 2.Sham; 3.Ischemia; 4.Ischemia+reperfusion; 5.DiOHF+Ischemia; 6.Ischemia+ DiOHF + reperfusion. The highest tissue glutathione levels were found in groups 5 and 6. Groups 1 and 2, which were control and sham groups respectively, had the lowest tissue GSH values. Ischemia group was found to have the highest tissue malondialdehyde (MDA) level. Tissue MDA levels in group 4 were lower than those in group 3, however, higher than the levels in all other groups. Erythrocyte GSH levels in groups 5 and 6 were higher than the levels in all other groups. Group 4 has highest plasma MDA values. Plasma MDA levels in group 3 were lower than the levels in Group 4, but higher than those in other groups. The results of the study indicate that intraperitoneal DiOHF administration inhibits lipid per oxidation that intensifies in the case of renal ischemia-reperfusion injury in rats.Öğe The Effect of Pinealectomy and Melatonin Application on Metallothionin, ZnT3 and ZIP2 Levels in Rat Brain Tissue(Karger, 2018) Deniz, Arife; Baltaci, Saltuk Bugra; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa Cihat[Abstract Not Availabe]Öğe Effect of pinealectomy and melatonin supplementation on metallothionein, ZnT2, ZIP2, ZIP4 and zinc levels in rat small intestine(Taylor & Francis Ltd, 2021) Unal, Omer; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa CihatWe investigated the relations among levels of metallothionein (MT); zinc (Zn) transport proteins, ZnT2, ZIP2 (ZRT and IRT-like proteins); and ZIP4, which enable Zn absorption in the small intestine of rats. We also investigated tissue Zn levels in the small intestine. We used four groups of adult male rats: group 1, control; group 2, pinealectomy (Px); group 3, Px + melatonin (MEL); group 4, MEL only. Animals in groups 3 and 4 were administered 5 mg/kg/day MEL for four weeks. At the end of the study, all animals were sacrificed and samples of duodenum, jejunum and ileum were harvested to analyze ZnT2, ZIP2, ZIP4 and MT levels using immunohistochemistry, and tissue Zn levels were measured by atomic absorption spectrophotometry. The lowest ZnT2 levels in the duodenum, jejunum and ileum, and the lowest ZIP2 levels in the duodenum and ileum were found in group 2. The lowest ZIP4 levels were found in the duodenum and jejunum, and the lowest MT levels in the duodenum and ileum were found in group 2. The highest MT values in the ileum were found in group 4. We found that ZnT2, ZIP2, ZIP4 and MT levels were reduced in the ileum compared to controls following Px, but levels approached control values after MEL administration. By its effects on ZnT2, ZIP2, ZIP4 and MT levels, MEL participates in the absorption of Zn in the rat small intestine.Öğe Effect of resveratrol administration on muscle glycogen levels in rats subjected to acute swimming exercise(C M B Assoc, 2019) Bicer, Mursel; Baltaci, Saltuk Bugra; Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim; Avunduk, Mustafa CihatThe present study aims to examine how resveratrol administration affects muscle glycogen levels in rats subjected to an acute swimming exercise bout. The study was conducted on adult male rats of Wistar-Albino. The 28 rats used in the study were equally divided among four groups: Group 1, Control Group: The group fed on a standard diet and not subjected to any procedure. Group 2, Control Swimming Group: The group fed on a standard diet and subjected to an acute swimming exercise bout for 30 minutes at the end of the study. Group 3, Resveratrol Group: The group fed on a standard diet and given (10 mg/kg) resveratrol in drinking water for four weeks. Group 4, Resveratrol Swimming Group: The group fed on a standard diet, given (10 mg/kg) resveratrol in drinking water for four weeks and subjected to a 30-minute acute swimming exercise at the end of the study. At the end of the four weeks, the animals were decapitated, muscle glycogen levels using immunohistochemical method. The highest muscle glycogen levels were obtained in the resveratrol-administered Group 3 and the lowest levels in group 2 (swimming group) (p<0.05). The results of the study demonstrate that resveratrol support had a protective and/or regulatory effect on mucle glycogen in both exercised and not-exercised rats.Öğe Effect of resveratrol administration on muscle glycogen levels in rats subjected to acute swimming exercise(C M B Assoc, 2019) Bicer, Mursel; Baltaci, Saltuk Bugra; Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim; Avunduk, Mustafa CihatThe present study aims to examine how resveratrol administration affects muscle glycogen levels in rats subjected to an acute swimming exercise bout. The study was conducted on adult male rats of Wistar-Albino. The 28 rats used in the study were equally divided among four groups: Group 1, Control Group: The group fed on a standard diet and not subjected to any procedure. Group 2, Control Swimming Group: The group fed on a standard diet and subjected to an acute swimming exercise bout for 30 minutes at the end of the study. Group 3, Resveratrol Group: The group fed on a standard diet and given (10 mg/kg) resveratrol in drinking water for four weeks. Group 4, Resveratrol Swimming Group: The group fed on a standard diet, given (10 mg/kg) resveratrol in drinking water for four weeks and subjected to a 30-minute acute swimming exercise at the end of the study. At the end of the four weeks, the animals were decapitated, muscle glycogen levels using immunohistochemical method. The highest muscle glycogen levels were obtained in the resveratrol-administered Group 3 and the lowest levels in group 2 (swimming group) (p<0.05). The results of the study demonstrate that resveratrol support had a protective and/or regulatory effect on mucle glycogen in both exercised and not-exercised rats.Öğe The effect of resveratrol supplementation on plasma leptin and liver glycogen levels in rats with acute swimming exercise(Wiley-Blackwell, 2015) Duran, Mursel Oguzhan; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa Cihat; Ergene, Neyhan[Abstract Not Availabe]Öğe Effect of Zinc and Melatonin on Oxidative Stress and Serum Inhibin-B Levels in a Rat Testicular Torsion-Detorsion Model(Springer/Plenum Publishers, 2017) Semercioz, Atilla; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa CihatThe present study was aimed to examine the effects of 3-week zinc and melatonin administration on testicular tissue injury and serum Inhibin-B levels caused by unilateral testicular torsion-detorsion in rats. The study was performed on 60 Wistar Albino-type adult male rats. The animals were allocated to 6 groups in equal numbers. 1. Control; 2. Sham; 3. Ischemia-reperfusion; 4. Zinc + ischemia-reperfusion; 5. Melatonin + ischemia-reperfusion; 6. Zinc + melatonin + ischemia-reperfusion. Zinc and melatonin were administered before ischemia-reperfusion at doses of 5 and 3 mg/kg respectively, by intraperitoneal route for a period of 3 weeks. Testicular torsion-detorsion procedures consisted of ischemia for 1 h and then reperfusion for another hour of the left testis. Blood and testicular tissue samples were collected to analyze erythrocyte and tissue GSH and plasma and tissue MDA, Inhibin-B levels. The highest erythrocyte and testis GSH values were found in zinc, melatonin, and zinc + melatonin groups (p < 0.001). Torsion-detorsion group has significantly lower erythrocyte GSH levels and higher plasma MDA values (p < 0.001). Serum inhibin-B and spermatogenic activity levels in the torsion-detorsion group were also significantly lower than those in the other groups (p < 0.001). However, zinc-, melatonin-, and melatonin + zinc-supplemented groups have higher inhibin-B and spermatogenetic activity (p < 0.001). The results of the study show that zinc, melatonin, and melatonin + zinc administration partially restores the increased oxidative stress, as well as the reduced inhibin-B and spermatogenic activity levels in testes ischemia-reperfusion in rats. Suppressed inhibin-B levels in the testicular tissue may be a marker of oxidative stress.Öğe The Effect of Zinc Deficiency and Supplementation on Elements in the Kidney Tissue of Ovariectomized Rats: Histopathologic Changes(Verlag Hans Huber, 2017) Baltaci, Abdulkerim Kasim; Sunar, Fusun; Mogulkoc, Rasim; Toy, HaticeThe objective of the present study is to determine the effects of zinc deficiency in and zinc supplementation to ovariectomized rats on some elements in kidney tissue. The study included 40 Sprague-Dawley type adult female rats. The experimental animals were randomized into four groups with equal numbers as follows: Group 1: Control (10). Group 2: Ovariectomized control (10). Group 3: Ovariectomized + zinc supplemented (10). Group 4: Ovariectomized + zinc deficient (10). After the animals were decapitated at the end of the experiment, element levels were determined by Atomic Emission (ICP-AES) as mg/g/wet tissue for calcium, phosphate, zinc, aluminum, copper, iron, lithium, and manganese and mu g/g/wet tissue for magnesium in the kidney tissue. Additionally, the tissue samples were subjected to a histopathologic assessment. An examination of the study results showed that ovariectomy significantly reduced calcium, phosphorus, and zinc levels, while zinc supplementation to the rats following ovariectomy restored the reduced element levels to normal (0.10 +/- 0.03, 0.85 +/- 0.16, 0.11 +/- 0.03 vs 0.19 +/- 0.06, 1.86 +/- 0.18, 0.52 +/- 0.05). Group 4, which was both ovariectomized and fed on a zinc-deficient diet, had significantly lower aluminum, copper, and lithium values. Calcification, inflammation, and sclerotic changes in group 4, the group which was fed on a zinc-deficient diet, were greater in comparison to other groups (p < 0.05). Results of the study suggest that ovariectomy + zinc deficiency leads to calcification, inflammation, and sclerotic changes in renal tissue and significantly reduces element levels, whereas zinc supplementation after ovariectomy restores the lowered element levels to normal.Öğe The effect of zinc deficiency and zinc supplementation on element levels in the bone tissue of ovariectomized rats: Histopathologic changes(Taylor & Francis Ltd, 2014) Baltaci, Abdulkerim Kasim; Sunar, Fusun; Mogulkoc, Rasim; Acar, Musa; Toy, HaticeObjective: Study aimed to determine the effects of zinc supplementation/deficiency on the histological structure and elements levels in bone tissue in ovariectomized rats. Methods: The study included 40 Sprague-Dawley type adult female rats, divided as follows: Control, ovariectomized, ovariectomized + zinc supplemented, ovariectomized + zinc deficient groups. At the end of the study bone tissues (femur) were collected to determine the levels of calcium, phosphorus, magnesium, zinc, iron, aluminium, chrome, lithium, lead, nickel, and manganese. The bone tissue was examined for histopathology. Results: Ovariectomy leaded to significant decrease in magnesium. Zinc supplementation to ovariectomized rats restored the reduced calcium, phosphorus, zinc. However, zinc deficiency in ovariectomized rats further reduced calcium, phosphorus, zinc, and manganese levels. Zinc deficiency in ovariectomized significantly increased Al, Cr, Li, Pb, and Ni levels. Tissue integrity was impaired due to ovariectomy and zinc deficiency. Conclusion: Ovariectomy and zinc deficiency leads significant decreases elements of the bone.Öğe Effect of zinc sulfate supplementation on metallothionein levels in rat heart tissue with induced ischemia-reperfusion injury(Biointerface Research Applied Chemistry, 2018) Yazgan, Betul; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa Cihat; Sahna, EnginMetallothioneins are remarkable proteins with regard to their role in the regulation of intracellular zinc balance and mediation of the physiological effects of zinc, as well as their antioxidant effects. The objective of the present study is to examine how zinc supplementation impacts metallothionein levels in ischemia-reperfusion injury in rats. The study involved heart tissues obtained from 30 Wistar-Albino adult male rats already used in another project. Experimental animals were equally divided into three groups as follows: Group 1: Control (H-Cont); Group 2: Heart Ischemia/Reperfusion (H-I/R); and Group 3: Zinc supplemented Ischemia/Reperfusion (H/Zn-I/R). Cardiac ischemia-reperfusion procedures were carried out under general anesthesia. In the zinc-supplemented cardiac I/R group (Group 3), the animals were supplemented with 5 mg/kg/day intraperitoneal (i.p.) zinc sulfate per rat for 21 days. At the end of the procedures, all animals were decapitated and heart tissues were collected. The tissues were subjected to immunohistochemical staining procedures using rat metallothionein antibody. The stained preparations were photographed and cells stained with metallothionein were counted at a light microscope to calculate their percentages. The analyzed heart tissue samples of the groups did not have any significant difference in terms of their metallothionein levels. Results obtained from the study indicate that 21-day zinc supplementation does not have a critical effect on metallothionein synthesis in cardiac ischemia-reperfusion. This result may be attributed to the dose of zinc, length of supplementation and/or duration of ischemia-reperfusion.Öğe Effects of zinc supplementation on metallothionein levels in ischemic renal tissue(Taylor & Francis Ltd, 2020) Yazgan, Betul; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa CihatWe investigated how zinc (Zn) supplementation affects metallothionein levels in the cortex and medulla of ischemic renal tissue of rats. We used adult male rats divided into four groups: group 1, untreated control; group 2, sham-operated; group 3, ischemia-reperfusion; group 4, ischemia-reperfusion + 5 g/kg Zn. Renal tissue was analyzed using immunostaining of rat metallothionein. Cells stained with metallothionein were counted and their percentage was calculated. We found that the Zn supplemented ischemia and reperfusion group exhibited a greater percentage of cells stained strongly for metallothionein in the renal cortex than all other groups. In the renal medulla, percentages of weak staining for metallothionein in the control and ischemia and reperfusion groups were greater than those in the sham and Zn-supplemented ischemia/reperfusion groups. Our findings indicate that the main effect of Zn in the renal tissue occurs in the cortex, while metallothionein synthesis in the renal medulla is unaffected.Öğe Resveratrol does not affect leptin while it has regulatory effects on liver glycogen levels in exercised and non-exercised rats(Verlag Hans Huber, 2019) Baltaci, Abdulkerim Kasim; Duran, Mursel Oguzhan; Mogulkoc, Rasim; Oltulu, Pembe; Avunduk, Mustafa CihatResveratrol (RES) is a well-known phytocompound and food component which has antioxidative and multifunctional bioactivities. The present study aims to examine how resveratrol administration affects plasma leptin and liver glycogen levels in rats subjected to an acute swimming exercise bout. The study was carried out on Wistar-Albino type adult male rats, each group include 7 rats. Group 1, Control Group. Group 2, Control Swimming Group: The group fed on a standard diet and subjected to an acute swimming exercise bout for 30 minutes at the end of the study. Group 3, Resveratrol Group: The group fed on a standard diet and given (10 mg/kg) resveratrol in drinking water for four weeks. Group 4, Resveratrol + Swimming Group: The group fed on a standard diet, given (10 mg/kg) resveratrol in drinking water for four weeks and subjected to a 30-minute acute swimming exercise at the end of the study. Plasma leptin levels using ELISA method (ng/l) and liver glycogen levels were determined by using histochemical method (number/0.1 mm(2)). Four weeks resveratrol administration to exercised and not-exercised rats did not cause a change in plasma leptin levels. Liver glycogen levels were 17.00 +/- 3.16; 14.12 +/- 2.98; 20.82 +/- 1.97; 16.38 +/- 1.27 (mean +/- sd); respectively in groups 1, 2, 3, 4. Resveratrol administration to rats subjected to a bout of acute swimming exercise produced an effect that prevented the decrease in liver glycogen (p < 0.05). The study highlights that resveratrol supplementation may have regulatory effects on liver glycogen levels in exercised and non-exercised rats.Öğe Role of Melatonin Receptors in Hyperthermia-Induced Acute Seizure Model of Rats(Humana Press Inc, 2019) Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim; Aydin, LeylaMelatonin is a neurohormone that has anticonvulsant activity in different experimental seizure models including hyperthermic febrile seizure. However, the mechanisms of this effect are not clear at the receptor level. The aim of the study was to determine which melatonin receptors involve in the hyperthermic febrile seizure model. 22-30 days Wistar male rats were used, and in children, it corresponds to 1.5-2 years. Groups were performed as (1) control, (2) ethanol/saline, (3) DMSO, (4) melatonin (MT), (5) MT + luzindole (LUZ), (6) MT + K-185, (7) MT + prazosin (PRZ), (8) MT + LUZ + K-185, (9) MT + LUZ + PRZ, (10) MT + K-185 + PRZ, and (11) MT + LUZ + PRZ + K-185. The hyperthermic febrile seizure pattern was established by keeping the rats in 45 degrees C hot water, and the latency, duration, and severity of seizures were determined in all groups. MT, LUZ, K-185, and PRZ were given 15, 45, 15, and 30 min before the induction of seizure, respectively. It was observed that melatonin shortened the duration of seizure, reduced the severity, and did not affect latency and that these effects were not completely blocked by receptor antagonists when compared with control, ethanol/saline, and DMSO groups. In conclusion, the fact that the anticonvulsant effect of melatonin is not completely blocked by all melatonin receptor antagonists. We can conclude that a multimodal mechanism may be responsible for the effect of melatonin receptors alone on the anticonvulsant effect of melatonin. It will be useful to design new pharmacological studies to make the subject clear.Öğe Zinc is a strong stimulant of metallothionein synthesis in the ischaemic testis tissue(Wiley, 2021) Yazgan, Betul; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa CihatThis study was performed to determine the effect of zinc supplementation effects on metallothionein levels in testis ischaemia-reperfusion of rats. The experimental groups were designed as Control, Sham, Ischaemia-Reperfusion (I/R) and I/R + Zinc supplemented. Zinc supplemented as 5 mg/kg day for 3 weeks. Testis tissues were analysed for metallothionein by immunohistochemical staining procedures. Group comparison showed that the zinc-supplemented ischaemia-reperfusion group had a significantly higher level of cells strongly stained with metallothionein than all other groups. A general evaluation of the results suggests that zinc supplementation is a strong stimulant of metallothionein synthesis in the ischaemic testis tissue.
