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Öğe ELA-DRA gene haplotypes in healthy Arabian horses(Elsevier Science Bv, 2016) Bulut, Zafer; Kurar, Ercan; Bulut, Oya; Nizamlioglu, Mehmet[Abstract Not Availabe]Öğe Genetic Diversity of Eight Domestic Goat Populations Raised in Turkey(Hindawi Ltd, 2016) Bulut, Zafer; Kurar, Ercan; Ozsensoy, Yusuf; Altunok, Vahdettin; Nizamlioglu, MehmetThe objective of this study was to determine the intra- and intergenetic diversities of eight different goat populations in Turkey including Hair, Angora, Kilis, Yayladag, Shami, Honamli, Saanen, and Alpine. A total of 244 DNA samples were genotyped using 11 microsatellites loci. The genetic differentiation between breeds was considerable as a result of the statistically significant (P < 0.001) pairwise F-ST values of each pair of breeds. Exceptionally, F-ST values calculated for Honamli and Hair breeds were statistically nonsignificant (P > 0.05). Heterozygosity values ranged between 0.62 and 0.73. According to the structure and assignment test, Angora and Yayladag goats were assigned to the breed they belong to, while other breeds were assigned to two or more different groups. Because this study for the first time presented genetic data on the Yayladag goat, results of structure analysis and assigned test suggest that further analyses are needed using additional and different molecular markers.Öğe Phylogenetic relationships of native Turkish cattle breeds using microsatellite markers(Tubitak Scientific & Technological Research Council Turkey, 2019) Ozsensoy, Yusuf; Kurar, Ercan; Dogan, Muge; Bulut, Zafer; Nizamlioglu, Mehmet; Altunok, Vahdettin; Isik, AyseA total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, respectively. TGLA122 was the most polymorphic locus; however, only 7 different alleles were observed for INRA005. A total of 40 different private alleles were determined. The general F-IS values were between 0.034 and 0.123. Due to the close location to the domestication center, higher genetic diversities were observed. The observed genetic diversities and the results of the phylogenetic analyses were in agreement with evolutionary history and the geographical origins of Turkish native cattle breeds.Öğe Sex-dependent colonic microbiota modulation by hazelnut (Corylus avellana L.) dietary fiber(Royal Soc Chemistry, 2023) Dastan, Elanur; Celik, Omer F.; Bas, Orhan; Bulut, Zafer; Lindemann, Stephen R.; Tugay, Mehmet I.; Degermenci, MuhammetAlthough many efforts have been made to characterize the functional properties of hazelnut constituents (mainly its oil, protein, and phenolics), those of its dietary fiber (DF) have not been elucidated yet. Here, we aimed to investigate the impact of DF of natural and roasted hazelnuts, and hazelnut skin on the colonic microbiota in vivo (C57BL/6J mouse models) by determining their composition through 16S rRNA sequencing and microbial short-chain fatty acids (SCFAs) using gas chromatography. Our results revealed that hazelnut DF generally showed an acetogenic effect in male mice, whereas the same trend was not observed in the female counterparts. The 16S rRNA sequencing results showed that hazelnut DF, especially that of natural hazelnuts, increased the relative abundances of Lactobacillus-related OTUs that have probiotic potential. LEfSe analysis indicated that, for female mice, Lachnospiraceae, Prevotella, Ruminococcaceae, and Lactobacillus were found to be discriminators for DF of natural hazelnuts, roasted hazelnuts, hazelnut skin, and control, respectively, whereas Bacteroides, Lactobacillus, Prevotella, and Lactococcus were the discriminators for the male counterparts, respectively. This study clearly indicates that, although the roasting process slightly alters the functionalities, hazelnut DF favors beneficial microbes and stimulates beneficial microbial metabolites in the colon in a sex-dependent way, which could be a contributing factor to the health-promoting effects of hazelnuts. Furthermore, hazelnut skin, a byproduct of the hazelnut industry, was found to have potential to be utilized to produce functional DF targeting colonic health.Öğe Türkiye’de bulunan bazı sığır ırklarının DGAT1 ve PRNP gen polimorfizminin araştırılması(2017) Şahin, İclal; Bulut, Zafer; Kurar, Ercan; Özşensoy, Yusuf; Doğan, Müge; Nizamlıoğlu, MehmetAmaç: Sunulan çalışmada, Türkiye yerli sığır ırklarında DGAT1 geni K232A ile PRNP geni promotor ve intron 1 bölgelerinde indel polimorfizmleri araştırılmıştır.Gereç ve Yöntem: Çalışmada materyal olarak, Boz Irk (BI), Yerli Kara (YK), Doğu Anadolu Kırmızısı (DAK), Güney Doğu Anadolu Kırmızısı (GAK) ve Zavot ırklarından olmak üzere toplam 122 kan örneği kullanıldı. Örnekler üzerinde DGAT1 geni K232A polimorfizmi Polimeraz Zincir Reaksiyonu- Parça Uzunluk Polimorfizmi (PZR-RFLP) tekniği kullanılarak ve PRNP geninin promotor bölgesi (23 bç indel) ile intron 1 bölgesi (12 bç indel) insersiyon-delesyon (indel) polimorfizmi PZR tekniği kullanılarak belirlendi. Bulgular: DGAT1 geninde A allelin en yüksek sıklığı YK (0,58) daha sonra GAK ve Zavot'da (0.50), en düşük sıklığı ise BI ve DAK'da (0.38) gözlendi. PRNP promotor ve intron 1 indel polimorfizmi için yapılan genotiplemede; promotor bölgesi en yüksek del/del genotipi sıklığı DAK ve GAK (sırası ile 0.53-0.52), en düşük BI ve Zavot (sırası ile 0.19 ve 0.17) ırklarında, intron 1 bölgesi del/del genotipi en yüksek sıklığı ise BI ve GAK (sırası ile 0.25-0.13), daha sonra YK (0.08) en düşük sıklığı DAK ve Zavot (0.05)'da görüldü.Öneri: BI, YK, DAK, GAK ve Zavot ırklarında yürütülen bu çalışma ile elde edilen verilerin, bu sığır ırklarında süt özelliği ile DGAT1 ve PRNP gen polimorfizmi arasındaki korelasyon ile ilgili çalışmalarda yararlı olacağı, hayvan yetiştiriciliği ve ıslah çalış- malarında süt verimi yönünden seleksiyona katkı sağlayacağı düşünülmektedir.
