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    Brain-Derived Neurotrophic Factor Levels in Mesenchymal Stem Cell Culture Fluid Under Different Culture Conditions
    (Aves, 2023) Cicek, Gulsemin; Cicek, Muharrem; Ozen, Emine Utlu; Aktan, T. Murad; Ozdengul, Faik; Duman, Selcuk
    Objective: Mesenchymal stem cells are used in various fields, such as cellular therapy, regeneration, or tissue engineering. It has been shown that they exhibit many protective factors and also work as a modulating chief within the region in which they are administered. There are studies on both the therapeutic and neuroprotective effects of brain-derived neurotrophic factor. Also, there are many studies on the improvement of culture conditions for in vitro reproduction of mesenchymal stem cells, which can be obtained from many sources in various bodies, such as adipose tissue and Wharton's jelly. Improving and standardizing these culture conditions will increase the effectiveness and reliability of stem cell therapies. Studies evaluating many culture conditions, such as O-2 level, type of medium, monolayer culture, and the transition from in vitro 3D models, are ongoing. Materials and Methods: In our study, groups were formed by using stem cells originating from adipose tissue and Wharton's jelly. Stem cell cultures were made using Hillex-II and Pronectin-F microcarriers. Cell culture O-2 level was adjusted as 1% and 5% for each group separately. Enzyme-linked immunosorbent assay was used to analyze brain-derived neurotrophic factor levels in stem cell culture fluid. Results: The highest brain-derived neurotrophic factor level in mesenchymal stem cells culture medium was observed in an adipose-derived stem cell culture with an in vitro fertilization (non-treated) dish, using a Hillex microcarrier in a 1% O 2 microenvironment. Conclusion: As a result of our observations, we think that cells could exhibit greater therapeutic potential in a dynamic adhesion environment.
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    Evaluation of the Effects of Adolescent Obesity on Electrocardiography and Pulmonary Function Tests
    (Galenos Publ House, 2022) Akkus, Yasin; Dibeklioglu, Saime Ergen; Ozdemir, Rahmi; Bas, Veysel Nijat; Cicek, Muharrem
    Introduction: The frequency of obesity is increasing in our country and all over the world. Obesity can also increase mortality and morbidity by affecting vital vital systems such as the cardiovascular system and the respiratory system. In this sense, early recognition of obesity and prevention of complications are of vital importance. In our study, we aimed to evaluate the effects of adolescent obesity on patients with obesity and normal by compairing electrocardiography (ECG) and pulmonary function tests (PFT) findings. Materials and Methods: Adolescent cases between 10 and 18 years of age were included in our study. Cases were divided into three groups according to body mass index. ECG and PFT measurements of the patients were obtained and study data were obtained. The data were evaluated with SPSS package. Results: One hundred fifty six patients were included in our study. In our study, no significant difference was found between ECG parameters in terms of heart rate (p=0,107). When Qt and QTc groups were compared, a statistically significant difference was found (p<0.001). There was no significant difference in QT dispersion and QTc dispersion (p=0.314, p=0.624, respectively). There was a significant difference between the groups in terms of Tp-e (p<0.05). There was no significant difference between the groups in terms of Tp-e/QT ratio and Tp-e/QTc ratio (p=0.054 and p=0.058). When the values of FEV1, FEV1/FVC, PEF, FEF25-75 were compared, a statistically significant difference was found between all groups (p<0.001). Conclusion: In our study, we concluded that the effects of obesity on respiratory and cardiovascular system in adolescents can be determined by ECG and PFT and possible complications can be prevented.