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    Efficacy of Light Emitting diodes (LEDs) Lighting System for In vitro Shoot Regeneration of Medicinal Water hyssop (Bacopa monnieri L. PENNEL)
    (Ars Docendi, 2018) Karatas, Mehmet; Aasim, Muhammad; Dazkirili, Murat
    Efficacy of different Light Emitting Diodes (LEDs) on in vitro whole plant regeneration of water hyssop (Bacopa monnieri L. Pennel) was investigated in this study. Shoot tip explants were cultured on MS medium with BA (0.25, 0.50 and 1.0 mg l(-1)) and incubated under Red: Blue (R:B) LEDs in different combinations (4:1, 3:1, 2:1 and 1:1 R: B and white LEDs) for 10 weeks. Callus induction and shoot regeneration was noted on all explants irrespective of LEDs type or BA concentration. Shoots per explant and shoot length ranged 6.56-10.42 and 0.96-1.71cm respectively. Maximum number of 10.42 shoots with highest shoot length of 1.71 cm was achieved under 1:1 R:B LEDs. Whereas, different BA concentrations generated 4.97-11.98 shoots per explant with shoot length in the range of 0.93-1.35 cm. Maximum shoots per explant (10.35) with more longer shoots (1.31 cm) was achieved on MS medium containing 1.0 mg l(-1) BA. Comparing of BA x LEDs, maximum number of 15.17 shoots per explant was induced under 1.0 mg l(-1) BA x 2:1 R: B LEDs. Whereas, longer shoots (2.68 cm) were scored under 0.5 mg l(-1) BA x 2:1 R: B LEDs. Regenerated shoots were rooted using 1.0 mg l(-1) IBA in four weeks followed by acclimatization in the aquariums provided with tap water. Results revealed that LEDs in combination with BA can be successfully employed for multiple shoot regeneration of water hyssop.