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Öğe The Efficiency of Hepatitis C Virus Core Antigen Test in the Diagnosis of Hepatitis C Infection(Galenos Yayincilik, 2016) Demircili, Mehmet Emin; Ozdemir, Mehmet; Feyzioglu, Bahadir; Baysal, BulentObjective: It was aimed to investigate diagnostic value of hepatitis C virus (HCV) core antigen test in patients with positive or negative anti-HCV assay by comparing with HCV ribonucleic acid (RNA) assay. Materials and Methods: Serum samples obtained from 189 patients who were admitted to Necmettin Erbakan University Meram Faculty of Medicine between December 2010 and February 2012, and HCV RNA assay were carried out for various reasons. Two mL of samples were stored under suitable conditions and anti-HCV, HCV core antigen and strip immunoblot assay [Commercial INNO LIA (TM) HCV Score (Innogenetics NV in Ghent, Belgium)] were performed. Genotyping was performed in the amplicons of the samples with positive HCV RNA test. Results: The diagnostic sensitivity specificity, negative predictive value and positive predictive value of HCV core antigen test were 96.2%, 100%, 97.3%, and 100%, respectively. Sixty-five serum samples were genotyped and their distribution were detected: Fifty-nine samples were genotype 1b, 2-genotype 1a/1b, 1-genotype 3a, 1-genotype 4, 1-genotype 2a/2c, and 1 was genotype 1a. Conclusion: It was concluded that HCV core antigen assay is highly specific, sensitive, reliable, reproducible, and easy to perform. It may be applied as a supplemental and confirmatory test in anti-HCV assays in the diagnosis of HCV.Öğe Hepatit C Enfeksiyonunun Tanısında Hepatit C Virüsü Kor Antijen Testinin Etkinliği(2016) Demircili, Mehmet Emin; Özdemir, Mehmet; Feyzioğlu, Bahadır; Baysal, BülentAmaç: Hepatit C virüsü (HCV) kor antijen testinin tanı değerinin antiHCV testi pozitif veya negatif olan hastalarda HCV ribonükleik asit (RNA) ile kıyaslanarak araştırılmasıdır.Gereç ve Yöntemler: Necmettin Erbakan Üniversitesi Üniversitesi Meram Tıp Fakültesi'ne Aralık 2010- Şubat 2012 tarihleri arasında başvuran ve çeşitli nedenlerle HCV RNA testi çalışılan 189 hastadan elde edilen serum örnekleri bu çalışmaya dahil edildi. İki mL serum örneği uygun koşullar altında saklandı ve anti HCV, HCV kor antijen ve strip immünblot testi [Ticari INNO LIA(TM) HCV Score testi (Innogenetics NV in Ghent, Belçika)] testleri çalışıldı. HCV RNA pozitif olan örneklere genotipleme yapıldıBulgular: Çalışmamızda HCV kor antijen testinin sensitivite, spesifite; negatif prediktif değer ve pozitif prediktif değerleri sırayla %96,2, %100, %97,3 ve %100 olarak tespit edildi. Genotipleme yapılan 65 örneğin 59'u genotip 1b, 2'si genotip 1a/1b, 1'i genotip 3a, 1'i genotip 4, 1'i genotip 2a/2c ve 1'i genotip 1a olarak tespit edildi.Sonuç: HCV kor antijen testi sensitivitesi ve spesifitesi yüksek, kolay uygulanabilir, güvenilir bir testtir. Bu test HCV enfeksiyonunun tanısında anti-HCV test sonuçlarının konfirmasyon ve tamamlayıcı testi olarak kullanılabilir.Öğe Investigating the effect of gold nanoparticles on hydatid cyst protoscolices under low-power green laser irradiation(Turkish Surgical Assoc, 2019) Colak, Bayram; Aksoy, Faruk; Yavuz, Selman; Demircili, Mehmet EminObjective: Various scolicidal agents are applied for the destruction of protoscolices in cysts media. Undesirable complications of the scolicidal agents limit the techniques to treat the cyst disease. Therefore, new non-toxic scolicidal agents are needed. Upon laser light irradiation, the photothermal gold nanoparticles (AuNPs) convert the absorbed laser light into heat through photothermal effect which kills the surrounding protoscolices by rising the temperature of the cysts media. In this study, we introduced biocompatible AuNPs as a non-toxic scolicidal agent to cure liver hydatid cysts. Material and Methods: The protoscoleces were collected from the livers of naturally infected sheeps. In each experimental group, 1.5 mL suspensions of hydatid liquid containing protoscolices were added to test tubes. The test tubes were divided into five groups. Control, AuNPs only, Green laser only, High-dose AuNPs + laser and Low-dose AuNPs + laser groups. Two concentrations (0.4 and 0.8 mL) of AuNPs and three laser powers (30, 50, 150 mW) were applied for 30, 60 and 120 minutes to the groups. Then the ciysts liquid assessed under a light microscope and determined the viability of protoscoleces. Results: Protoscolices in high-dose AuNPs group were destructed up to 89.30% deaths under 150 mW laser power for 120 minutes. However, negligible cell deaths were observed in cases where only AuNPs added or only laser irradiated groups. Increasing the dose of AuNPs or laser power or duration of aplication increased the protoscolosidal death rate. Conclusion: In the study, we have successfully demonstrated that the AuNPs are an effective therapeutic and scolicidal agent to cure hydatid cyst disease under laser irradiation.Öğe Rapid Dissemination of Multidrug Resistant Providencia Stuartii- A University Hospital Based Study(Allied Acad, 2014) Feyzioglu, Bahadir; Gueldemir, Dilek; Karagoz, Alper; Erayman, Ibrahim; Demircili, Mehmet Emin; Baykan, MahmutProvidencia stuartii infections are not common. There are a few reports of declared outbreaks or spreads. In our study, nosocomial dissemination by extended-spectrum beta-lactamase (ESBL) producing Providencia stuartii in a variety of clinics was investigated in a short period. Nine multidrug-resistant Providencia stuartii clinical isolates were collected between October and November in 2011. Nine isolates were obtained from six patients who had been hospitalized in a variety of clinics at a university hospital in Turkey. The clonal relationship among nine isolates was determined by pulsed-field gel electrophoresis. All isolates were ESBL positive and resistant to quinolones, and we determined amikacin resistance to three strains. There were two antibiotypes, and a second PFGE profile was not observed in different types. The outbreak was due to the dissemination of one epidemic multiresistant clone of Providencia stuartii. We considered that it had been a common dissemination from a single source and the first isolate which was collected from the first patient was epidemic clone. The respiratory tract colonization of the first patient was considered to be the possible route of transmission. The resistance to amikacin occurred during treatment with aminoglycoside. It was noteworthy.Öğe The Use of Radiofrequency Thermal Ablation Method in The Treatment of Hepatic Hydatid Cysts: Ex vivo Sheep Study(2019) Sarıcık, Bekir; Kartal, Adil; Esen, Hacı Hasan; Demircili, Mehmet EminObjective: Hydatid disease is a disease caused by parasites belonging to the echinococcus family. This disease is often caused byEchinococcus granulosus and rarely by echinococcus alveolaris.The parasite may cause illness anywhere in the human body, mainlyin liver. In this study, we aimed to destroy the hydatid cyst viability by Radiofrequency Thermal Ablation (RFTA) method whichhas been used in many areas in medicine.Methods: We used fresh sheep liver with hydatid cysts. Average diameter of cysts was 3.3 cm. The study was performed in 3groups, each of which involved 20 cysts. After more than half of the cyst fluid was drained, ablation was performed. When thecore temperature of the cyst exceeded 95C, ablation procedure was continued for 3 minute in 1st group and for 4 minutes in 2ndgroup. Third group was the control group. And then, cyst fluid and germinative membrane were collected for microbiologic andpathologic assessment.Results: In 1st group, the cysts could not be destroyed at the desired level. In 2nd group, it was observed that 100% of theprotoscolex died and 100% of the germinative membranes was degenerated. In control group, %13 of protoscolex died and %10of germinative membranes wasdegenerated.Conclusion: We destroyed all the protoscolex and germinative membranes by using RFTA in 2nd group.
