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Öğe Antibiotic Susceptibility of Group A B-Hemolytic Streptococci Isolated From Tonsillar Swab Samples in 5-15 Years Old Children(Modestum Ltd, 2014) Dogan, Metin; Aydemir, Ozlem; Guner, Sukru Nail; Feyzioglu, Bahadir; Baykan, MahmutTonsillopharyngitis is a common infectious disease in the pediatric age group. Group A Beta Hemolytic Streptococcus ( GAS) is a main infectious agent of pharyngitis. Optimal therapeutic approach in these patients has been a matter of debate to avoid the complications of infection. The aim of this study was to investigate of antibiotic susceptibility for group A B-hemolytic Streptococci which isolated from tonsillar swab samples of 5-15 years old children. 2599 outpatient children who are 5-15 years old and have general infection symptoms like high fever, weakness, chills-shivering and observed hyperemia at tonsils and pharynx were included in the study. Tonsillopharyngeal culture and antimicrobial susceptibility tests were performed. 319 ( 12,3%) GAS isolates was obtained from tonsillopharyngeal cultures of the 2599 patients. Susceptibility to penicillin, vancomycin, linezolid, cefotaxime, erythromycin, chloramphenicol, and clindamycin were 100%, 100%, 100%, 100%, 97,2%, 98,3%, and 94,7% respectively. Resistance to penicillin was not yet observed, penicillin may safely be chosen to treatment of these infections for non-allergic patients to penicillin. It is considered that culture and antibiotic susceptibility testing may be more useful to for the diagnosis and treatment of these patients.Öğe Antibiotic Susceptibility of Group A B-Hemolytic Streptococci Isolated From Tonsillar Swab Samples in 5-15 Years Old Children(Modestum Ltd, 2014) Dogan, Metin; Aydemir, Ozlem; Guner, Sukru Nail; Feyzioglu, Bahadir; Baykan, MahmutTonsillopharyngitis is a common infectious disease in the pediatric age group. Group A Beta Hemolytic Streptococcus ( GAS) is a main infectious agent of pharyngitis. Optimal therapeutic approach in these patients has been a matter of debate to avoid the complications of infection. The aim of this study was to investigate of antibiotic susceptibility for group A B-hemolytic Streptococci which isolated from tonsillar swab samples of 5-15 years old children. 2599 outpatient children who are 5-15 years old and have general infection symptoms like high fever, weakness, chills-shivering and observed hyperemia at tonsils and pharynx were included in the study. Tonsillopharyngeal culture and antimicrobial susceptibility tests were performed. 319 ( 12,3%) GAS isolates was obtained from tonsillopharyngeal cultures of the 2599 patients. Susceptibility to penicillin, vancomycin, linezolid, cefotaxime, erythromycin, chloramphenicol, and clindamycin were 100%, 100%, 100%, 100%, 97,2%, 98,3%, and 94,7% respectively. Resistance to penicillin was not yet observed, penicillin may safely be chosen to treatment of these infections for non-allergic patients to penicillin. It is considered that culture and antibiotic susceptibility testing may be more useful to for the diagnosis and treatment of these patients.Öğe Comparative evaluation of automated chemiluminescence tests and RIBA assay used in HCV diagnosis(Scientific Publishers India, 2016) Kalem, Fatma; Yuksekkaya, Serife; Dagi, Hatice Turk; Ertugrul, Omur; Dogan, MetinIntroduction: Hepatitis C, caused by hepatitis C virus (HCV) can be a mild illness lasting a few weeks or can cause lifelong liver cirrhosis and cancer. Today although the sensitivity of diagnostic tests is increasing; it has often been associated with decreased specificity so the rate of false-positive test results is increasing. The aim of this study was to compare the false-positive rates of anti-HCV results. Methods: During the period of 18.07.2011 to 18.12.2013; blood samples of patients admitted to Konya Numune Hospital were screened for anti-HCV using chemiluminescence immunoassay (CIA). After 2012; the new version of same anti-HCV test was used. Borderline and reactive results were retested and tests which were reactive in repeated CIA were confirmed by a recombinant immunoblot-assay (RIBA). Subjects with a positive RIBA test were considered to have been as true positive anti-HCV. Results: A total of 54178 sera were tested for anti-HCV during the period of 18.07.2011 to 18.12.2013 and 649 sera were positive with chemiluminescence method. 374 of reactive cases were confirmed by RIBA. The RIBA results showed 171 (45.7 %) negative, 163 (43.5 %) positive, and 40 (10.7 %) indeterminate results. By using the new version of the test; the rate of false positive and indeterminate anti-HCV test results decreased from 75.1% to 35.5 %. Conclusions: In this study it was observed that lower false positive rates of newly developed test. Lowering the false positive rate of ELISA tests will provide more confidence to use these tests in the diagnosis of HCV. There is a need for further studies on this issue.Öğe Comparison of a New and Rapid Method: Brucella Coombs Gel Test With Other Diagnostic Tests(Wiley, 2016) Kalem, Fatma; Ergun, Ayse Gul; Durmaz, Suleyman; Dogan, Metin; Ertugrul, Omur; Gundem, SevalBackground: The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis. Methods: Brucella Coombs gel test (BCGT), Brucella ELISA (IgG IgM), Standard agglutination test, and Brucella immunocapture agglutination tests of 78 patients with presumptive diagnosis of brucellosis which were sent to Microbiology Laboratory of Konya Numune Hospital from various regions of Konya were studied. Results: Results: Of 78 patients with ELISA IgG and IgM, STA, BICA and BCGT; 26, 21, 10, 12 and 12 were positive. When compared with BICA, the sensitivity and specifity of BCGT were 100% and 100%, respectively. Conclusion: According to results BCGT can be used as a diagnostic test in routine laboratories after more comprehensive studies in control groups and patients. J. Clin. Lab. Anal. 30:756-759,2016. (C) 2016 Wiley Periodicals, Inc.Öğe Comparison of the performance of TK system with LJ and MGIT methods in the diagnosis of tuberculosis(E-Century Publishing Corp, 2014) Feyzioglu, Bahadir; Dogan, Metin; Sanli, Ozlem O.; Ozdemir, Mehmet; Baykan, MahmutTuberculosis is a common infectious disease caused by various strains of mycobacteria, usually Mycobacterium tuberculosis (TB). Various liquid or solid media are used for the diagnosis of tuberculosis. TK Rapid Mycobacterial Culture System has been developed recently. In our study, we aimed to compare TK Rapid Mycobacterial Culture System with LJ and MGIT systems in the diagnosis of tuberculosis. 200 clinical specimens (152 sputum, 41 Bronchoalveolar lavage fluid (BAL), 4 gastric aspirations, 2 urine and 1 wound) obtained from 192 patients from different clinics were included for the diagnosis of TB. All specimens were decontaminated by using the same-common procedure in all the methods. The obtained sediment was used for inoculation for the BACTEC MGIT 960, TK and LJ. Additionally, smears were prepared from the residual suspension for Ehrlich-Ziehl-Neelsen (EZN) staining for microscopic examination. Contamination was observed in 23 sputum and 4 BAL samples. Contamination rates for TK, LJ, and BACTEC MGIT 960 systems were determined as 3 (1.5%), 13 (6.5%), and 18 (9%) respectively. Mycobacterium tuberculosis growth was determined as 15 (7.5%), 14 (7%) and 13 (6.5%) by TK culture system, MGIT and LJ, respectively. In our study, the total mean detection times of Mycobacterium tuberculosis by the LJ, TK, and MGIT method were 20.1, 17.1, and 8.3 days, respectively. TK system showed a dramatically lower contamination rate than the others. There was no difference in growth rates for each of the three methods. We concluded that the TK culture system is disadvantageous in terms of turnaround time.Öğe Comparison of Various Methods for Identification of Anaerobic Bacteria and Investigation of Sensitivity to Antibiotics(Bilimsel Tip Yayinevi, 2022) Ugrakli, Selin; Dogan, MetinIntroduction: In this study, it was aimed to identify anaerobic bacteria isolated from clinical specimens admitted to Necmettin Erbakan University Meram Faculty of Medicine Hospital, Microbiology Laboratory with suspicion of anaerobic infection via different methods and to determine the antibiotic susceptibility against several antibiotics.Materials and Methods: The study included 197 clinical specimens sent for anaerobic culture between August 2019 and March 2020. The samples were incubated using anaerobic culture techniques and the isolates obtained were identified by conventional methods, an-ident discs, API 20 a panel and Vitek 2 semi-automatic system compatible ANC cards. Penicillin G, cefoxitin, clindamycin, metro-nidazole, imipenem, piperacillin/tazobactam, ceftolozane /tazobactam susceptibilities of isolates were determined by concentration gradient method.Results: A total of 46 anaerobic bacteria were isolated from 37 (18.8%) samples; 69.6% of these isolates were gram-positive anaerobic bacteria and 30.4% were gram-negative anaerobes. The most frequently isolated anaerobic bacteria were Clostridium spp. (n= 9) and Peptostreptococcus spp. (n= 8) in gram-positives; Prevotella spp. (n= 7) and Bacteroides fragilis group (n= 5) in gram-negatives. The compatibility between API20A panels and ANC cards was determined 79.5% (35/44), 43.2% (19/44) of test strains at the genus and species levels respectively. The highest rate of resistance was detected against penicillin G (38.6%). Cefoxitin, clindamycin, metronidazole, piperacillin/tazobactam and imipenem sensitivity rates were 93.2%, 65.9%, 84.1%, 93.2% and 100% respectively. The MIC value of ceftolozane-tazobactam, one of the new antimicrobials, varied in a wide range (0.032-12mg/L) according to the bacterial species. While the highest MIC values were found in B. fragilis group strains, the lowest levels were detected in Veillonella spp. (MIC= 0.25) and Fusobacterium spp. (MIC= 0.5 mg/L) isolates.Conclusion: As a result, the data of this study, which is one of the limited number of studies evaluating the performance among anaerobic diagnostic tests frequently used in routine; suggested that the diagnosis should be supported by a different diagnostic test in case of inconsistency between the identification result and clinical or antibiotic susceptibility profiles. One of our isolates was the first strain of metronidazole resistant B. fragilis isolated in our laboratory in which requires monitoring of susceptibility profiles. To our knowledge, this is the first study to evaluate the efficacy of ceftolozane/tazobactam on anaerobic clinical isolates from our country. Ceftolozane/tazobactam activity was detected in great variability between bacterial species in accordance with the literature data. So, it is need optimized studies including more bacterial species and numbers.Öğe Comperative Evaluation of Vitek 2 and Etest Methods with the Referance Broth Microdilution for Antimicrobial Susceptibility Testing of Colistin Among Multi-Drug Resistant Gram-Negative Bacteria(Bilimsel Tip Yayinevi, 2021) Ugrakli, Selin; Dogan, MetinIntroduction: Despite the increased need for colistin, especially in serious infections caused by carbapenem resistant gram-negative bacteria, problems and challenges regarding colistin susceptibility testing remain. The aim of this study was to evaluate the performance of Vitek 2, one of the commonly used automated systems, and Etest for colistin susceptibility testing compared with reference broth microdilution method (BMD). Materials and Methods: This study included 657 multi-drug resistance (MDR) Gram negative bacteria obtained from clinical samples; Negative control, Escherichia coli ATCC 25922 and Positive control, Escherichia coli NCTC 13846. The collected MDR isolates were performed colistin BMD according to ISO standard 20776-1, prospectively. Categorical agreement (CA), Very Major Error (VME), and Major Error (ME) rate were calculated. Acceptable performance was evaluated as; CA >= 90%; VME <1.5% and ME <3%. Results: Colistin resistance rates were detected by Vitek 2, Etest and BMD; 40.3%, 48.7%, 53.9%, respectively. CA rates were as fol- lows: Vitek 2 92.4% and Etest 71.9%. While the compatibility of Vitek 2 and BMD was observed (kappa value= 0.85) to be 'excellent agreement'; the agreement of Etest and BMD was found to be 'moderate' (kappa value= 0.45). Although CA varied from 85.7% to 100% for Vitek 2, it ranged 63.6% to 80% for Etest depending on bacterial species. Alarming high rates of VME were determined for Vitek 2 (14.5%) and Etest (36.5%). While MEs were 1.7% by Vitek 2; there was no false resistant isolate with Etest. Conclusion: It may be recommended for laboratories not to rely on Vitek 2 and Etest colistin susceptibility results. Additionally, colistin resistant isolates will be underestimated by reducing colistin susceptibility studies to a specific minimum inhibitory concentration (MIC). In this regard, our suggestion is that laboratories would improve their infrastructure and staff skills to apply BMD routinely.Öğe Determination of antibiotic resistance and high-performance liquid chromatography profiles for Mycobacterium species(Wiley, 2018) Ozer, Turkan Toka; Yula, Erkan; Dogan, Metin; Baskin, HuseyinBackgroundIncidence of mycobacterial infections has been increasing. However, diagnosis and treatment of mycobacterial infections can be difficult. The aim of this study was to investigate high-performance liquid chromatography (HPLC) analysis of the mycolic acids for rapid identification and dendrogram cluster analysis of mycobacterium species. MethodsClinical specimens received for mycobacterial culture and antimicrobial susceptibility test were processed by standard laboratory protocols. Positive cultures were analyzed with HPLC method. Mycolic acid analysis with HPLC was used for diagnosis of tuberculosis and other mycobacterial infections. These reports were compared with Sherlock Library mycobacterial species, and the similarity index was analyzed. This value was formed by a software in multidimensional space that was the calculation of the average distance between the nearest library profile and unknown profile. ResultsThe ninety-two samples were identified as M.tuberculosis. (similarity index between 0.593 and 0.994). One of the other strains was identified as M.avium intracellulare (strain No. 82) (SI=0.906); one of them was identified as M.interjectum (strain no. 89) (SI=0.644). Total 94 samples were identified, and dendrogram was applied to these samples. Profile A (10.6%), profile B (59.6%), profile C (11.7%), profile D (3.2%), and other profiles as single different profiles were identified. Rates for each as 1% (89, 94, 1, 82, 26, 42, 32, 41, 100, 43, 47, 44, 40, 35). ConclusionHigh-performance liquid chromatography is a useful, rapid, reliable, and practical method for diagnosis of mycobacterium species.Öğe Determination of Carbapenemase Producing of Klebsiella pneumoniae Isolated in Blood Cultures By Matrix Assisted Laser Desorption/Ionization-flight Time Mass Spectrometry(Bilimsel Tip Yayinevi, 2020) Dogan, Metin; Baba, Abdullah YucelIntroduction: Klebsiella pneumoniae can cause Healthcare Related Infections (HRIs), especially by carbapenemase producing microorganisms. Rapid methods are needed to determine carbapenem resistance. MALDI-TOF MS is a faster diagnostic method than conventional methods. It is expected that routine use and standardization of this diagnostic test in laboratories will contribute to the rapid determination of carbapenemase resistance. In this study, it was aimed to determine carbapenemase enzyme production by MALDI-TOF MS technique in K. pneumoniae strains. Materials and Methods: Carbapenem susceptible (n=40) and non-susceptible (n=96) K. pneumoniae strains isolated in the Medical Microbiology Laboratory between January 2016 to December 2017 were included into the study. Detecting of production of carbapenemase enzyme was investigated by MALDI-TOF MS technique by comparing with the polymerase chain reaction (PCR). Results: PCR analysis showed that 93 of the carbapenem resistant isolates had OXA-48 gene and three of them had NDM gene. In the MALDI-TOF MS analyzes of the 96 isolates with genotypic resistance, 88 were resistant and three were susceptible. Five isolates of carbapenem resistant with PCR could not be identified as resistant or sensitive by this method. Forty isolates susceptible to carbapenem were determined to be susceptible by MALDI-TOF MS. Sensitivity, specificity, positive predictive, negative predictive values were 96.7%, 100%, 100%, 93% respectively. Conclusion: It appears that MALDI-TOF MS determines carbapenemase production with high specificity and sensitivity. We think it will be beneficial to complete standardization studies and use them in routine practice.Öğe Determining the Infectious Pathogens and Their Resistance to Antibiotics in a Pediatric Intensive Care Unit(Georg Thieme Verlag Kg, 2018) Akin, Fatih; Yazar, Abdullah; Dogan, MetinIntroduction Nosocomial infections are one of the main causes of morbidity and mortality. It is important to know the common infectious pathogens and their resistance profiles in intensive care units (ICUs) to determine appropriate treatment protocols. The aimof this study was to determine the epidemiological profile of microorganisms isolated in a pediatric ICU (PICU) and to determine antibiotic resistance in isolated strains. Materials and Methods This retrospective study was performed at the Meram Medical Faculty Hospital, Necmettin Erbakan University, Konya, Turkey. A total of 1,502 bacteria that were isolated from various specimens from children who were hospitalized in PICUs between January 2014 and December 2015 were included in this study to determine the isolated bacteria diversity and susceptibility to various antibiotics. Results Staphylococcus spp. was the most frequently isolated microorganism followed by Escherichia coli and Klebsiella spp., respectively. The sites where pathogens were isolated were as follows: 616 blood, (41%), 445 urine (29.6%), 60 sputum (4%), 44 cerebrospinal fluid (2.9%), 25 wound swab (1.6%), 20 tracheal aspirate (1.3%), and 26 others (1.7%). The carbapenem resistance rate was 40.8% among Pseudomonas aeruginosa isolates. Among 60 Acinetobacter baumannii isolates tested, 62% were resistant to carbapenems. Sensitivity rates of A. baumannii isolates to tigecycline and colistin were as high as 98 and 96%, respectively. Meropenem and colistin resistance rates to Klebsiella spp. were 16.2 and 15%, respectively. Conclusion In conclusion, it is essential to identify the infectious pathogens and their resistance to antibiotics especially in ICUs where infections with multidrug-resistant bacteria are frequent. Studies on this issue should be performed at appropriate time intervals.Öğe The Effect of Different Dental Implant Surface Characteristics on Bone Immunologic Biomarkers and Microbiologic Parameters: A Randomized Clinical Study(Quintessence Publishing Co Inc, 2021) Karci, Bilge; Oncu, Elif; Dogan, MetinThis study assessed the levels of tumor necrosis factor-alpha (TNF-alpha), prostaglandin E2 (PGE2), receptor activator of nuclear factor kappa B (RANK), RANK ligand (RANKL), osteoprotegerin (OPG), and levels of Fusobacterium nucleatum, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Prevotella intermedia, and Streptococcus oralis in areas where airborne particle-abraded, large-grit, acid-etched (SLA), fluorine-modified, and anodized implant surfaces are used. A total of 71 implants from 37 patients were assessed, grouped according to the surface characteristics of the implants: SLA surface (Group 1), fluorine-modified surface (Group 2), and anodized surface (Group 3). The following clinical indices were measured: Gingival Index (GI), probing depth (PD), bleeding on probing (BOP), clinical attachment level (CAL), and keratinized tissue width (KTW). Peri-implant sulcus fluid and subgingival plaque samples were also collected. Commercial enzyme-linked immunosorbent assay (ELISA) kits were purchased for measuring TNF-alpha, PGE2, RANKL, RANK, and OPG. Real-time quantitative polymerase chain reaction (PCR) was used to detect P intermedia, T forsythia, T denticola, F nucleatum, P gingivalis, and S oralis levels in the subgingival biofilms. The groups showed no statistically significant differences in GI, PD, BOP, CAL, KTW, or peri-implant status. The total amounts of PGE2, TNF-alpha, RANKL, RANK, and OPG and the RANKL/OPG ratio were not significantly different between groups. F nucleatum, T forsythia, P intermedia, P gingivalis, and T denticola were significantly higher in Group 3 implants. DNA concentrations of S oralis were higher in Group 2. Within the limitations of this study, SLA and fluorine-modified implant surfaces may be more clinically successful than anodized-surface implants.Öğe Investigation of Bacterial Gastroenteritis Agents in Patients Who Admitted to Pediatric Clinic with Diarrhea(Doc Design Informatics Co Ltd, 2021) Agrali, Muhammet Sukru; Dogan, MetinObjective: In this study we aimed to determine bacterial causes of diarrhea in patients presenting to the pediatric clinic. Methods: The stool specimens of 487 patients with diarrhea from pediatric emergency and other pediatric clinics were investigated in order to determine gastroenteritis causes. Microscopic examinations, and appropriate culture methods were performed to determine Salmonella, Shigella, E. coil O157: H7, Campylobacter, Yersinia enterocolitica, Vibrio cholerae, and Aeromonas species. Stool specimens of patients with diarrhea were stained with methylene blue for microscopic examination, and they were cultured on propagating selenite F medium for Salmonella spp. and Shigellas spp., and 8 h later, were subcultured to Salmonella Shigella agar. Also they were cultured to hektoen and xylose lysine desoxycholate agar, to Sorbitol MacConkey agar for E. cob O157: H7 strain, to MacConkey agar for Yersinia enterocolitica, to Campylobacter selective agar for Campylobacter spp., to vibrio selective agar for Vibrio cholerae and to 5% sheep blood agar for Aeromonas spp.. The isolated microorganisms were identified by conventional methods, biochemical tests, specific antibody/antigens and VITEK* 2 (bioMerieux, Marcy l'Etoile, France) device. Results: Salmonella spp. was the most frequently isolated bacteria that was isolated in 19 (3.9%) samples, and Campylobacter spp. was isolated in seven (1.4%), and E. coil O157:H7 and Aeromonas spp. were isolated in three (0.6%) samples. We observed that Salmonella isolation was highest in HE agar. It is remarkable that E. coil O157: H7 was observed among the bacterial agents of gastroenteritis in children. Conclusions: We concluded that E. coil O157: 117 strain should be investigated especially in stool samples with bloody diarrhea in our region, and bacteria such as Campylobacter spp. Aeromonas spp. should be investigated in addition to Salmonella spp. for investigation of gastroenteritis agents in children.Öğe Investigation of granulomatous prostatitis incidence following intravesical BCG therapy(E-Century Publishing Corp, 2014) Balasar, Mehmet; Dogan, Metin; Kandemir, Abdulkadir; Taskapu, Hakan Hakki; Cicekci, Faruk; Toy, Hatice; Gurbuz, RecaiIn the present manuscript, we studied the incidence of granulomatous prostatitis in the prostatectomy specimen of the patients who underwent transurethral resection of the prostate (TURP) after superficial bladder cancer treatment with intravesical Bacillus Calmette-Guerin (BCG) and were diagnosed with benign prostate hyperplasia (BPH). The clinical data and histopathological specimen records of 472 patients who underwent TUR-P due to BPH diagnosis, obtained over a period of 6 years in the urology department of Private Konya Hospital, Konya, Turkey, were studied retrospectively. The cases were divided into two groups as (Group I) who did not undergo any treatment and as (Group II) who underwent BCG treatment. The frequency and the clinical course of the cases with granulomatous prostatitis were studied histopathologically. There were in total 472 patients who underwent TUR-P. Out of the 459 patients who did not undergo BCG treatment (Group I), the histopathological specimen records of 262 (57%) was BPH, of 197 (43%) BPH + chronic prostatitis. Of the second group, 13 cases underwent intravesical BCG treatment before surgical intervention due to superficial bladder CA diagnosis. In this group 4 of the cases were diagnosed as (30%) BPH, 9 as (70%) chronic prostatitis + BPH. 6 out of the 9 chronic prostatitis cases were chronic prostatitis, 2 caseous granulomatous prostatitis, 1 non-caseous granulomatous prostatitis. Granulomatous prostatitis cases should require no specific therapy. Conclusion: In patients with obstruction complaints following intravesical BCG treatment, granulomatous prostatitis should also be considered and treatment plans should be made accordingly.Öğe The presence of Torque teno virus in chronic obstructive pulmonary disease(E-Century Publishing Corp, 2014) Feyzioglu, Bahadir; Teke, Turgut; Ozdemir, Mehmet; Karaibrahimoglu, Adnan; Dogan, Metin; Yavsan, MehmetTorque Teno Virus (TTV) has been identified as transfusion-transmitted virus in humans, initially. Although TTV viremia is extremely common in the general population worldwide, there is no direct causal evidence linking TTV infection to specific clinical manifestations. Our hypothesis was that TTV might play a role in Chronic obstructive pulmonary disease (COPD) by inducing inflammatory mechanisms previously identified. The study was conducted on 57 COPD patients and 39 healthy control groups. COPD patient groups included: the patients (n:20) with exacerbation needed noninvasive ventilation, the patients (n: 19) who received only medical treatment, and the invited patients (n: 18) for outpatient control. Serum samples were collected from patients and voluntary blood donors. TTV DNA quantification was carried out with a real time PCR by the hybridization probe system and viral load was interpreted through the crossing point value. TTV DNA was detected in the majority of both patients and healthy control groups. The prevalence was 94.4% (17/18) in patients for outpatient control, 94.7% (18/19) in patients who received only medical treatment, 100% (20/20) in patients with exacerbation needed noninvasive ventilation and 84.6% (33/39) in healthy controls. This difference was not statistically significant. However, CP values was statistically different in all the patient groups from the control group. TTV DNA prevalence was higher in patients than healthy individuals. More interesting thing, viral load was highest in the patients with exacerbation needed noninvasive ventilation. As a result, TTV may be associated with COPD and the severity of it.Öğe Rare Cause of Soft Tissue Infection: Shewanella algae(Georg Thieme Verlag Kg, 2024) Yilik, Ezgi; Akgul, Ayse Humeyra; Genceli, Mustafa; Akcan, Ozge Metin; Kan, Seda Nur Arazi; Dogan, MetinShewanella algae is a common microorganism in nature, but a rare pathogen in humans. It is a causative agent of skin and soft tissue infections, especially in patients with skin ulcers or trauma. S. algae can enter the body through the eyes, ears, gastrointestinal tract, or respiratory tract. Immunocompromised individuals are at increased risk of developing bacteremia and sepsis from the infection. This report presents a case of posttraumatic soft tissue infection caused by S. algae in an 18-year-old male after swimming in the sea. The bacteria were cultured from the wound site.Öğe A Rare Complication during Salmonella sp. Infection in a 4-Year-Old Child: Acute Pancreatitis(Georg Thieme Verlag Kg, 2021) Kilic, Ahmet O.; Akin, Fatih; Yazar, Abdullah; Cokbicer, Abdulkerim; Dogan, Metin; Energin, Vesile M.Objective We aimed to report a rare complication of acute pancreatitis which developed during Salmonella infection. Methods We report a 4-year-old girl with pancreatitis that occurred after salmonella gastroenteritis. Discussion Salmonella sp. infections cause serious mortality and morbidity worldwide. Salmonella transmission is mostly fecal oral from contaminated water and foods. Acute pancreatitis expresses the acute inflammatory process of the pancreas. Inflammation of the pancreas associated with bacterial, viral, and fungal agents can lead to acute pancreatitis. There are limited data regarding the frequency of infections leading to pancreatitis. Here, a pediatric case with pancreatitis that occurred after salmonella gastroenteritis is reported. Conclusion Acute pancreatitis due to S. sp. infection is very rare in children. Pancreatitis should be considered in patients with gastroenteritis with typical abdominal pain.Öğe A Rare Presentation of Sphingomonas paucimobilis in a Healthy Child: Acute Ischemic Stroke(Georg Thieme Verlag Kg, 2023) Yilmaz, Busra Zeynep; Akcan, Ozge Metin; Pekcan, Sevgi; Parlatan, Havva; Emlik, Ganime Dilek; Dogan, MetinSphingomonas paucimobilis is a rarely pathogenic organism that usually infects immunocompromised patients and causes nosocomial infections, but a few community-acquired infections have been identified in relatively healthy adult patients. Herein we report a 5-year-old child who presented with acute ischemic stroke caused by S. paucimobilis that was treated successfully with antibiotics and antithrombotic agents. According to our knowledge, this is the first case of acute ischemic infarction with S. paucimobilis in a previously healthy child.Öğe The role of torque teno virus (TTV) viremia in sarcoidosis etiology(Kuwait Medical Assoc, 2019) Teke, Turgut; Feyzioglu, Bahadir; Tosun, Mustafa; Dogan, Metin; Yesildag, Kerim; Ozdemir, MehmetObjective: To investigate a possible role of torque teno virus (TTV) in the etiology of sarcoidosis Design: Controlled prospective study Setting: Faculty of Meram Medicine, Necmettin Erbakan University, Konya, Turkey Subjects: This cross-sectional, clinical study included 37 sarcoidosis patients and 9 healthy control patients. Intervention: TTV DNA quantification was performed by real-time PCR by the hybridization probe system, and viral load was interpreted by means of the crossing point (CP) value in sera of subjects. Main outcome measures: The prevalence and viral load of TTV Results: TTV DNA was detected in the majority of both patients and healthy controls. The prevalence was 59.5% in patients and 55.6% in controls. This difference was not statistically significant. However, CP values were statistically different in the patient group from the control group (p < 0.001); namely, the viral load of TTV was higher in the patient group than in the healthy control group. Conclusions: Overall, these findings demonstrate for the first time that TTV plays a role in the etiology of sarcoidosis. It is likely that the role of TTV in the pathogenesis of sarcoidosis is an aberrant immune response, driven by viral antigens.
