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    A new molecular approach to the diagnosis of small ruminant morbillivirus with EvaGreen based assay
    (Tubitak Scientific & Technological Research Council Turkey, 2021) Oz, Mustafa Emin; Torlak, Emrah; Avci, Oguzhan; Dogan, Muge; Uncu, Ali Tevfik
    The present work describes a new method that is anticipated to contribute to the global control and eradication strategies of small ruminant morbillivirus (SRMV), which negatively affects sheep and goat breeding in many countries where outbreaks emerge. Described assay was developed to detect SRMV by targeting the fusion glycoprotein gene. In the study, the EvaGreen based real-time RT-PCR method is compared with real-time RT-PCR based on TaqMan probe, which is recommended as the diagnostic test by World Organisation for Animal Health (OIE). Detection limit, normalized fluorescence values, and sensitivity of the methods were evaluated in the analysis. The lowest limit of detection obtained was approximately 12 RNA copies per reaction corresponding to a C-T value of 36.79. According to the results, it was observed that the developed in-house method has a specific, sensitive, and powerful detection limit as much as the gold standard test. As a result, it was concluded that the proposed method, which was developed as a more cost-effective and easily optimized analysis, could provide an advantage for detecting SRMV in diagnostic laboratories.
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    Phylogenetic relationships of native Turkish cattle breeds using microsatellite markers
    (Tubitak Scientific & Technological Research Council Turkey, 2019) Ozsensoy, Yusuf; Kurar, Ercan; Dogan, Muge; Bulut, Zafer; Nizamlioglu, Mehmet; Altunok, Vahdettin; Isik, Ayse
    A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, respectively. TGLA122 was the most polymorphic locus; however, only 7 different alleles were observed for INRA005. A total of 40 different private alleles were determined. The general F-IS values were between 0.034 and 0.123. Due to the close location to the domestication center, higher genetic diversities were observed. The observed genetic diversities and the results of the phylogenetic analyses were in agreement with evolutionary history and the geographical origins of Turkish native cattle breeds.