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    Antibacterial nano cerium oxide/chitosan/cellulose acetate composite films as potential wound dressing
    (Pergamon-Elsevier Science Ltd, 2020) Kalaycioglu, Zeynep; Kahya, Nilay; Adimcilar, Veselina; Kaygusuz, Hakan; Torlak, Emrah; Akin-Evingur, Gulsen; Erim, F. Bedia
    Novel chitosan and cellulose acetate polymer composites with nanosized cerium oxide were prepared as potential wound dressing materials. Chitosan and cellulose acetate are natural polymer derivatives with biocompatible properties. Both polymers can be dissolved in formic acid. The polymer composite was formed by solvent-casting method. The formed films were not soluble in water. In order to improve antibacterial, thermal, and mechanical properties of the composite material, films were loaded with nanosized cerium oxide. Films were characterized with thermogravimetric, scanning electron microscopy with energy-dispersive X-ray spectroscopy, mechanical, ultraviolet-visible light transmittance, water solubility, pH, moisture content, water vapor transmission rate, swelling, and antibacterial tests. The physical properties and antibacterial characteristics of the films are promising for further research as a potential wound covering material.
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    Antimicrobial and physical properties of chitosan films incorporated with turmeric extract
    (Elsevier Science Bv, 2017) Kalaycioglu, Zeynep; Torlak, Emrah; Akin-Evingur, Gulsen; Ozen, Ilhan; Erim, F. Bedia
    In this study, the effects of turmeric extract incorporation on the antibacterialand physical properties of the chitosan films were evaluated. Turmeric containing chitosan-based film was produced with casting procedure and cross-linked with sodium sulfate. Mechanical, optical, thermal properties, and water vapor permeability of the films were studied. The addition of turmeric to chitosan film significantly increased the tensile strength of the film and improved the ultraviolet-visible light barrier of the film. Infrared spectroscopy analysis suggested an interaction between the phenolic compounds of the extract and amin group of chitosan. Antimicrobial activity of the chitosan films was studied against Salmonella and Staphylococcus aureus by plate count agar technique and a better antimicrobial activity was observed with turmeric incorporation. Turmeric incorporated chitosan films with enhanced antimicrobial activity and film stiffness can be suggested as a promising application for food packaging. (C) 2017 Elsevier B.V. All rights reserved.
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    Antimicrobial cerium ion-chitosan crosslinked alginate biopolymer films: A novel and potential wound dressing
    (Elsevier Science Bv, 2017) Kaygusuz, Hakan; Torlak, Emrah; Akm-Evingur, Gulsen; Ozen, Ilhan; von Klitzing, Regine; Erim, F. Bedia
    Wound dressings require good antiseptic properties, mechanical strength and, more trustably, natural material ingredients. Antimicrobial properties of cerium ions and chitosan are known and alginate based wound dressings are commercially available. In this study, the advantages of these materials were combined and alginate films were crosslinked with cerium(III) solution and chitosan added cerium(III) solution. Films were characterized by Fourier transform infrared spectroscopy (FTIR), light transmittance, scanning electron microscopy (SEM), swelling expefiments, water vapor transmittance tests, and mechanical stretching tests. The antibacterial and physical properties of the films were compared with those of conventional calcium alginate films. Both cerium ion crosslinked and cerium ion-chitosan crosslinked alginate films gained antibacterial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Cerium alginate-chitosan films showed high resistance to being deformed elastically. Results show that cerium alginate-chitosan films can be flexible, ultraviolet protecting, and antibacterial wound dressings. (C) 2017 Elsevier B.V. All rights reserved.
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    Comparative physical, antioxidant, and antimicrobial properties of films prepared by dissolving chitosan in bioactive vinegar varieties
    (Elsevier, 2023) Adimcilar, Veselina; Kalaycioglu, Zeynep; Akin-Evingur, Gulsen; Torlak, Emrah; Erim, F. Bedia
    Solvent casting following the dissolution of chitosan in aqueous acetic acid is the most widely used method for preparing chitosan films. In this study, an economical and practical way is proposed to improve the physico-chemical properties of chitosan films by using vinegar varieties both as solvents and as bioactive additives to improve the properties of the films. Chitosan films were prepared by dissolving chitosan in pomegranate, grape, apple, and hawthorn vinegar. Vinegar contains bioactive phenolics and different organic acids together with acetic acid, depending on the main raw material from which it is obtained. The films' mechanical, optical properties, antioxidant and antimicrobial activities were compared with each other and with the chitosan film prepared by dissolving chitosan in acetic acid. The antioxidant and antimicrobial properties of chitosan films prepared with vinegar increased. The use of vinegar as a solvent increased the UV light barrier properties of the films. Improved antimicrobial, antioxidant, optical, and elastic properties of films prepared by dissolving chi-tosan in vinegar varieties are promising in applications of these films as potential and economic food packaging materials.
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    Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films
    (Elsevier, 2021) Kizilkonca, Ezgi; Torlak, Emrah; Erim, F. Bedia
    Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated. Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method. Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation. Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA). Cerium oxide nanoparticle incorporation enhanced the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus. This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength. ? 2021 Elsevier B.V. All rights reserved.