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Öğe Antibiotic Susceptibility of Group A B-Hemolytic Streptococci Isolated From Tonsillar Swab Samples in 5-15 Years Old Children(Modestum Ltd, 2014) Dogan, Metin; Aydemir, Ozlem; Guner, Sukru Nail; Feyzioglu, Bahadir; Baykan, MahmutTonsillopharyngitis is a common infectious disease in the pediatric age group. Group A Beta Hemolytic Streptococcus ( GAS) is a main infectious agent of pharyngitis. Optimal therapeutic approach in these patients has been a matter of debate to avoid the complications of infection. The aim of this study was to investigate of antibiotic susceptibility for group A B-hemolytic Streptococci which isolated from tonsillar swab samples of 5-15 years old children. 2599 outpatient children who are 5-15 years old and have general infection symptoms like high fever, weakness, chills-shivering and observed hyperemia at tonsils and pharynx were included in the study. Tonsillopharyngeal culture and antimicrobial susceptibility tests were performed. 319 ( 12,3%) GAS isolates was obtained from tonsillopharyngeal cultures of the 2599 patients. Susceptibility to penicillin, vancomycin, linezolid, cefotaxime, erythromycin, chloramphenicol, and clindamycin were 100%, 100%, 100%, 100%, 97,2%, 98,3%, and 94,7% respectively. Resistance to penicillin was not yet observed, penicillin may safely be chosen to treatment of these infections for non-allergic patients to penicillin. It is considered that culture and antibiotic susceptibility testing may be more useful to for the diagnosis and treatment of these patients.Öğe Antibiotic Susceptibility of Group A B-Hemolytic Streptococci Isolated From Tonsillar Swab Samples in 5-15 Years Old Children(Modestum Ltd, 2014) Dogan, Metin; Aydemir, Ozlem; Guner, Sukru Nail; Feyzioglu, Bahadir; Baykan, MahmutTonsillopharyngitis is a common infectious disease in the pediatric age group. Group A Beta Hemolytic Streptococcus ( GAS) is a main infectious agent of pharyngitis. Optimal therapeutic approach in these patients has been a matter of debate to avoid the complications of infection. The aim of this study was to investigate of antibiotic susceptibility for group A B-hemolytic Streptococci which isolated from tonsillar swab samples of 5-15 years old children. 2599 outpatient children who are 5-15 years old and have general infection symptoms like high fever, weakness, chills-shivering and observed hyperemia at tonsils and pharynx were included in the study. Tonsillopharyngeal culture and antimicrobial susceptibility tests were performed. 319 ( 12,3%) GAS isolates was obtained from tonsillopharyngeal cultures of the 2599 patients. Susceptibility to penicillin, vancomycin, linezolid, cefotaxime, erythromycin, chloramphenicol, and clindamycin were 100%, 100%, 100%, 100%, 97,2%, 98,3%, and 94,7% respectively. Resistance to penicillin was not yet observed, penicillin may safely be chosen to treatment of these infections for non-allergic patients to penicillin. It is considered that culture and antibiotic susceptibility testing may be more useful to for the diagnosis and treatment of these patients.Öğe Changing Infection Dynamics with the Pandemic: Distribution of Viral Agents of Respiratory Tract Infections in the Last 5 Years(Galenos Publ House, 2023) Karabey, Mehmet; Kaya, Havva; Kaba, Kadir; Ceylan, Alperen; Taskin, Zekeriya; Ozdemir, Mehmet; Feyzioglu, BahadirIntroduction: The measures taken against Severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2) positively impacted the reduction of its transmission. In addition, these measures also significantly decreased the spread of infections caused by other respiratory viruses. In this study, we aimed to determine the frequency of respiratory virus infections, other than SARS-CoV-2, during the Coronavirus disease-2019 (COVID-19) pandemic and investigated their course during both the quarantine and normalization periods.Materials and Methods: Swab samples sent to Necmettin Erbakan University Meram Faculty of Medicine Hospital Medical Microbiology Laboratory between May 2017 and May 2022 to determine the viral agents of respiratory tract infections by polymerase chain reaction (PCR) were retrospectively scanned.Results: A total of 187,240 SARS-CoV-2 PCR tests were performed between April 1, 2020, and May 31, 2020, and 14,773 (9.82%) tests were reported as positive. Based on our observation, the viruses demonstrating a decrease during the pandemic period were influenza A and B, seasonal H1N1, human metapneumovirus, respiratory syncytial virus A and B, and human herpes virus 7. No changes were observed in the infection rates of parvovirus B19, adenovirus, and human rhinovirus.Conclusion: In our study, we observed a serious decline in the cases caused by other respiratory viral agents and the detection rates of these agents during the pandemic period compared to the pre-pandemic period. This can be attributed to the extensive impact of the measures implemented during the COVID-19 pandemic to mitigate the spread of respiratory infections. Our results are a reflection of this situation. We believe that the data obtained from a large number of samples will serve as a guide for managing infections during the current pandemic and for future experiences.Öğe Comparison of the performance of TK system with LJ and MGIT methods in the diagnosis of tuberculosis(E-Century Publishing Corp, 2014) Feyzioglu, Bahadir; Dogan, Metin; Sanli, Ozlem O.; Ozdemir, Mehmet; Baykan, MahmutTuberculosis is a common infectious disease caused by various strains of mycobacteria, usually Mycobacterium tuberculosis (TB). Various liquid or solid media are used for the diagnosis of tuberculosis. TK Rapid Mycobacterial Culture System has been developed recently. In our study, we aimed to compare TK Rapid Mycobacterial Culture System with LJ and MGIT systems in the diagnosis of tuberculosis. 200 clinical specimens (152 sputum, 41 Bronchoalveolar lavage fluid (BAL), 4 gastric aspirations, 2 urine and 1 wound) obtained from 192 patients from different clinics were included for the diagnosis of TB. All specimens were decontaminated by using the same-common procedure in all the methods. The obtained sediment was used for inoculation for the BACTEC MGIT 960, TK and LJ. Additionally, smears were prepared from the residual suspension for Ehrlich-Ziehl-Neelsen (EZN) staining for microscopic examination. Contamination was observed in 23 sputum and 4 BAL samples. Contamination rates for TK, LJ, and BACTEC MGIT 960 systems were determined as 3 (1.5%), 13 (6.5%), and 18 (9%) respectively. Mycobacterium tuberculosis growth was determined as 15 (7.5%), 14 (7%) and 13 (6.5%) by TK culture system, MGIT and LJ, respectively. In our study, the total mean detection times of Mycobacterium tuberculosis by the LJ, TK, and MGIT method were 20.1, 17.1, and 8.3 days, respectively. TK system showed a dramatically lower contamination rate than the others. There was no difference in growth rates for each of the three methods. We concluded that the TK culture system is disadvantageous in terms of turnaround time.Öğe COVID-19 Seroprevalance in a University Hospital Health Workers(Bilimsel Tip Yayinevi, 2021) Arslan, Gokce Kader; Ozdemir, Mehmet; Kaya, Havva; Feyzioglu, Bahadir; Kepenek Kurt, Esma; Erayman, IbrahimIntroduction: Healthcare workers are at the forefront in the Pandemic war against COVID-19 (Coronavirus Disease 2019) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2). In this struggle, they have become high-risk by keeping in close con- tact with patients during their diagnosis, treatment, and follow-up with long working hours. The aim of this study was to contribute to epidemiological data of our country by examining the antibody status of our hospital healthcare workers. Materials and Methods: Anti-SARS-CoV-2 IgG/IgM, COVID-19 ELISA kits were studied from sera samples of healthcare workers in Necmettin Erbakan University Meram Medical Faculty Hospital between June 1 and November 30, 2020. Nasopharyngeal swab sam- ples of these persons were also tested with the Real Time Polymerase Chain Reaction (RT-PCR) method. Results: SARS-CoV-2 seroprevalence of 741 healthcare workers included in our study was found to be 17%. Seropositivity was detected in 6.4% (33/515) of the healthcare workers with negative SARS-CoV-2 PCR test and in 3.9% (5/130) of the healthcare workers who did not have PCR test. Among the healthcare workers, the highest seroprevalence was observed in nurses (39.6%) followed by doctors (%23). Conclusion: It was evaluated that SARS-CoV-2 seroprevalence in healthcare workers is higher than in the population. This study shows that occupational exposure is a risk factor. 3.9% seropositivity was found in healthcare workers who never had a test. Considering that these workers have an asymptomatic or subclinical infection, there is a possible risk for nosocomial transmission. Therefore, healthcare professionals should use personal protective equipment and apply hygiene rules correctly and effectively in infectious diseases, especially during pandemic periods, while working in the hospital.Öğe Detection of influenza viruses from patients in university hospital(Elsevier Science Bv, 2016) Gulcen, Begum Saran; Feyzioglu, Bahadir; Ozdemir, Mehmet; Baykan, Mahmut[Abstract Not Availabe]Öğe The Efficiency of Hepatitis C Virus Core Antigen Test in the Diagnosis of Hepatitis C Infection(Galenos Yayincilik, 2016) Demircili, Mehmet Emin; Ozdemir, Mehmet; Feyzioglu, Bahadir; Baysal, BulentObjective: It was aimed to investigate diagnostic value of hepatitis C virus (HCV) core antigen test in patients with positive or negative anti-HCV assay by comparing with HCV ribonucleic acid (RNA) assay. Materials and Methods: Serum samples obtained from 189 patients who were admitted to Necmettin Erbakan University Meram Faculty of Medicine between December 2010 and February 2012, and HCV RNA assay were carried out for various reasons. Two mL of samples were stored under suitable conditions and anti-HCV, HCV core antigen and strip immunoblot assay [Commercial INNO LIA (TM) HCV Score (Innogenetics NV in Ghent, Belgium)] were performed. Genotyping was performed in the amplicons of the samples with positive HCV RNA test. Results: The diagnostic sensitivity specificity, negative predictive value and positive predictive value of HCV core antigen test were 96.2%, 100%, 97.3%, and 100%, respectively. Sixty-five serum samples were genotyped and their distribution were detected: Fifty-nine samples were genotype 1b, 2-genotype 1a/1b, 1-genotype 3a, 1-genotype 4, 1-genotype 2a/2c, and 1 was genotype 1a. Conclusion: It was concluded that HCV core antigen assay is highly specific, sensitive, reliable, reproducible, and easy to perform. It may be applied as a supplemental and confirmatory test in anti-HCV assays in the diagnosis of HCV.Öğe Emerging Technologies for the Diagnosis of Viral Infections and SARS-CoV-2(Gazi Univ, Fac Med, 2020) Altindis, Mustafa; Feyzioglu, BahadirCoronavirus disease 2019 (COVID-19) is a newly emerging infection caused by Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Considering that there is no specific drug or vaccine yet for COVID-19, effective rapid diagnosis of viruses has become very important in terms of early detection and control of the outbreak. The routine difficulties of isolating the virus necessitated the diagnosis to be made with more serological tests for many years. However, in recent years, molecular tests that provide fast and high-quality viral diagnosis information have started to take their place in laboratories. Syndrome-based PCR tests after PCR and multiplex PCR tests are also approaches that question the direct factor and accelerate the diagnosis and treatment. LAMP PCR technology has also developed rapidly, and the diagnosis time has been shortened in the field or at the bedside with very small portable devices. As a new technology, CRISPR diagnostic methods and portable DNA sequencing devices will be very useful in the diagnosis of viral infections in the clinic for rapid results per patient. With immunoprecipitation systems using luciferase-labeled antigens, virus identification, quantitation, antiviral efficacy can be monitored. COVID-19 outbreak management increased the need for very fast and reliable tests and triggered the laboratory biotechnology industry. The entire world is experiencing a dynamic pandemic process in which the benefits of new, highly sensitive, accessible and portable identification methods will be tested. The presence of a large number of applications in the approval process for these methods provides strong evidence that SARS-CoV-2 diagnostic algorithms will have richer and productive solutions in the near future. Experiences will be guiding in better understanding of other viral infections, establishing bedside diagnostic solutions, providing more effective treatments.Öğe Emerging Technologies for the Diagnosis of Viral Infections and SARS-CoV-2(Gazi Univ, Fac Med, 2020) Altindis, Mustafa; Feyzioglu, BahadirCoronavirus disease 2019 (COVID-19) is a newly emerging infection caused by Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Considering that there is no specific drug or vaccine yet for COVID-19, effective rapid diagnosis of viruses has become very important in terms of early detection and control of the outbreak. The routine difficulties of isolating the virus necessitated the diagnosis to be made with more serological tests for many years. However, in recent years, molecular tests that provide fast and high-quality viral diagnosis information have started to take their place in laboratories. Syndrome-based PCR tests after PCR and multiplex PCR tests are also approaches that question the direct factor and accelerate the diagnosis and treatment. LAMP PCR technology has also developed rapidly, and the diagnosis time has been shortened in the field or at the bedside with very small portable devices. As a new technology, CRISPR diagnostic methods and portable DNA sequencing devices will be very useful in the diagnosis of viral infections in the clinic for rapid results per patient. With immunoprecipitation systems using luciferase-labeled antigens, virus identification, quantitation, antiviral efficacy can be monitored. COVID-19 outbreak management increased the need for very fast and reliable tests and triggered the laboratory biotechnology industry. The entire world is experiencing a dynamic pandemic process in which the benefits of new, highly sensitive, accessible and portable identification methods will be tested. The presence of a large number of applications in the approval process for these methods provides strong evidence that SARS-CoV-2 diagnostic algorithms will have richer and productive solutions in the near future. Experiences will be guiding in better understanding of other viral infections, establishing bedside diagnostic solutions, providing more effective treatments.Öğe Frequency of Adenovirus and Rotavirus and Their Seasonal Distribution in Children With Gastroenteritis(Aves, 2016) Tuzuner, Ugur; Gulcen, Begum Saran; Ozdemir, Mehmet; Feyzioglu, BahadirObjective: In our study, we aimed to investigate the prevalence of rotavirus and enteric adenovirus in stool samples sent to our laboratory for antigen detection of children between 0-18 years of age admitted to hospital with diarrhea, abdominal pain, vomiting and fever and diagnosed as gastroenteritis. We also analyzed their frequencies according to demographic parameters. Methods: Results of 5156 pediatric patients admitted to Nec-mettin Erbakan University Meram Faculty of Medicine Hospital and diagnosed as gastroenteritis between January 2013-December 2015 were investigated retrospectively. VIKIA (R) Rota-Adeno (bioMerieux, Marcy l'Etoile, France), a chromatographic immunoassay detecting both viruses simultaneously was used according to the manufacturer's recommendations in stool samples. Results: Viral antigens were detected in 884 (17.1%) of the total 5156 samples. 764 (14.8%) of the positive results were detected as rotavirus and 120 (2.3%) were detected as adenovirus. Of the patients with positive results, 412 (46.6%) were female and 472 (53.4%) were male. When results are considered according to age, 2-4 age group was found to have the most common positivity (n=372) as 42.1%. Seasonal distribution of acute gastroenteritis cases was analyzed and the number of cases due to rotavirus was found to be increased in winter and spring and enteric adenoviruses were detected all year round. Conclusions: Rotavirus is the most common reason of gastroenteritis in the newborn and children, which must be considered for patients with diarrhea especially in the first four years of life. Rapid diagnosis is important for prediction of clinical implications and treatment. As enteric adenovirus is an important reason of gastroenteritis in infancy and childhood, it is necessary to investigate adenovirus antigens as well. Conducting regional studies are important for contributing to epidemiological data.Öğe Hepatitis C Genotypes in Patients with Chronic Hepatitis C Infection: A Three-Year Evaluation(Bilimsel Tip Yayinevi, 2020) Gulseren, Yasemin Derya; Esenkaya Tasbent, Fatma; Ozdemir, Mehmet; Feyzioglu, BahadirIntroduction: In case of chronic hepatitis C infection, cirrhosis and hepatocellular carcinoma may progress. HCV genotypes and subtypes have been found to vary according to geographical regions. In addition to its epidemiological importance, HCV genotype is an important factor in determining the response and duration of treatment. In this study, it was aimed to determine the genotype distribution in our region. Materials and Methods: The results of 241 patients with HCV RNA positivity detected in our laboratory Molecular unit between 2016 and 2018 were retrospectively screened. HCV-RNA extraction for genotyping was performed by automated system (EZ1 Virus Mini Kit v.2.0, Germany), and line probe assay (LIPA) based on reverse hybridization method was applied. HCV-RNA levels were determined by real-time PCR method (Artus HCV QS-RGQ kit, Qiagen, Germany). Results: Two hundred and forty-one patients were included in the study, and 116 (48%) were females and 125 (52%) were males. Mean age was 56.1 +/- 19.4 (range: 16-90) years. Mean logarithmic viral load value was 5.7 +/- 0.9 IU/ml (range; 2.71 x 10(2)-17 x 10(6)), mean value of AST was 50.5 +/- 43.7 IU/ml and mean ALT value was 63.4 +/- 63.5 IU/ml. Genotype 1b was detected in 58.9% of the patients, genotype 3a in 14.1%, genotype 1a in 13.27%, genotype 2b in 4.1%, genotype 4a in 1.2%. The subtypes could not be determined for 4.9%, 1.2%, 1.6% and 0.4% of infected patient in genotype 1,2,4 and 5 respectively. Conclusion: In our study, genotype 1b (58.9%) was found as the dominant genotype. This was followed by genotype 3a (14.1%). In patients infected with genotype 1, viral load value was found to be significantly higher than other genotypes. Monitoring genotype change is important for determining treatment protocols and duration.Öğe High thioredoxin reductase 1 expression in meningiomas undergoing malignant progression(Springer Japan Kk, 2015) Esen, Hasan; Feyzioglu, Bahadir; Erdi, Fatih; Keskin, Fatih; Kaya, Bulent; Demir, Lutfi SaltukThioredoxin (Trx) is a redox active protein that regulates several physiological and biochemical functions, such as growth, apoptosis and cellular defense. The function of Trx itself is regulated by thioredoxin reductase (TrxR). This study was designed to determine the expression of TrxR1 in meningioma tissues of different World Health Organization grades (grade I-III). Meningioma tissues were extracted from the histopathological specimens of 29 patients. These samples included seven histologically normal meningeal tissues that served as a control group and 12 grade I, 12 grade II and 5 grade III meningioma samples. TrxR1 expression was evaluated using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunostaining. The proliferative and apoptotic indices of the specimens were investigated by Ki-67 immunostaining and TUNEL assay, respectively. TrxR1 expression, as assessed by qRT-PCR, increased significantly with meningioma grade (p < 0.001). The immunostaining intensity of TrxR1 increased significantly with meningioma grade (p < 0.001). Ki-67 index values increased significantly in accordance with grade progression (p < 0.001). The apoptotic index values were not significantly different in any group (p > 0.05). Trx system seems to be involved in the malignant progression of meningiomas. Further, large studies are required to elucidate the exact role of this system.Öğe Increased expression of ubiquitin C-terminal hydrolase L1 in astrocytomas of ascending grades(Lippincott Williams & Wilkins, 2023) Izci, Emir Kaan; Keskin, Fatih; Erdi, Fatih; Kaya, Bulent; Karatas, Yasar; Feyzioglu, Bahadir; Findik, SiddikaBackground:The ubiquitin-proteasome pathway controls the monitoring and degradation of important proteins and is involved in several cellular processes, such as development, differentiation, and transcriptional regulation. Recent evidence has shown that ubiquitin carboxy-terminal hydrolase-L1 (UCH-L1), a member of the deubiquitinating enzyme family that removes ubiquitin from protein substrates, is overexpressed in many types of cancer. Aim:This study thus examined the expression of UCH-L1 in human astrocytoma tissues. Material and methods:Formalin-fixed, paraffin-embedded astrocytoma samples were obtained from 40 patients, after which histopathological examination, typing, and grading were performed. The study group included 10 histologically normal brain tissues, which served as the control group, and 10 WHO grade II, 10 WHO grade III, and 10 WHO grade IV (glioblastoma) samples. Normal brain tissue samples were obtained from the histologically normal, non-tumoral portion of the pathology specimens. UCH-L1 expression was evaluated using quantitative reverse transcription-polymerase chain reaction and immunohistochemistry. Results:Astrocytoma tissues exhibited higher UCH-L1 expression compared to the control group. UCH-L1 overexpression increased significantly together with the increase in astrocytoma grades (from II to IV). Conclusion:UCH-L1 could be a good diagnostic and therapeutic marker for determining astrocytoma development and progression.Öğe Investigation of Parvovirus B19 IgM and IgG Positivity Rates in Pediatric Hematology Patients(Georg Thieme Verlag Kg, 2018) Gorkem, Aysun; Ugur, Ayse Ruveyda; Ozdemir, Mehmet; Feyzioglu, Bahadir; Baykan, MahmutHuman parvovirus B19 is a frequent etiologic agent causing erythema infectiosum in children. It has recently been suggested that parvovirus B19 may be latent after infection and cause reactive infections especially in immunosuppressed patients with hematological problems. In this study, we aimed to investigate the parvovirus B19 immunoglobulin M (IgM) and immunoglobulin G (IgG) seropositivity rates of patients evaluated in a pediatric hematology clinic. We retrospectively screened the laboratory results of parvovirus B19 IgM and IgG antibody assays of children less than 18 years, who consulted pediatric in-and-outpatient clinics between 2013 and 2016. Parvovirus B19 IgM and IgG antibodies were investigated in serum samples by using enzymelinked immunosorbent assay method in the Medical Microbiology Laboratory. Parvovirus B19 IgM antibodies were detected in 109 of 602 patients attending pediatric hematology clinics (18.1%). Parvovirus B19 IgG antibody was detected in 244 of 952 patients attending pediatric hematology clinics (25.6%). Parvovirus B19 IgM and IgG positivity in samples from pediatric in-and-outpatient clinics other than pediatric hematology were 2.8% and 35.7%, respectively. Parvovirus IgM and IgG positivity in serum samples sent from the pediatric hematology clinic and outpatients was statistically significant compared with those sent from pediatric clinics other than pediatric hematology (p = 0.0001 and p = 0.0008, respectively). The higher detection rate of serum parvovirus B19 IgM positivity in patients under the follow-up of pediatric hematology clinics suggests that immune suppression-related viral reinfection or persistence may occur in these patients.Öğe Laboratory Diagnosis of Pediatric Herpesvirus Infections of the Central Nervous System by a Multiplex Polymerase Chain Reaction Assay and Intrathecal Antibodies(Georg Thieme Verlag Kg, 2018) Feyzioglu, Bahadir; Yavru, Sibel; Ozdemir, MehmetIntroduction Central nervous system (CNS) viral infections are a serious problem requiring accurate diagnosis and treatment. Human herpesviruses (HHVs) are an important cause of these infections. Recent research has focused on new diagnostic methods allowing accurate and rapid identification of viral infections because there are still diagnostic difficulties for these infections. This study was done to determine the etiologic role of human herpes viruses and to obtain information that will contribute to the diagnostic algorithm in suspected cases of viral encephalitis or aseptic meningitis. Materials and Methods In our study, herpes simplex virus (HSV)-1, HSV-2, varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and HHV-6 DNA was detected in the cerebrospinal fluid (CSF) by multiplex polymerase chain reaction (PCR) and virus-specific immunoglobulin G (IgG) antibodies in CSF and serum by EIA in pediatric encephalitis/meningitis cases. Results HSV-1 and VZV were detected in 5 and 3.3% of aseptic meningitis cases, respectively, but no encephalitis cases. Other viruses were not identified as etiologic agents. The seroprevalences were determined as 72.4, 34.3, 81.9, 93.3, 88.6, and 80.9%, respectively for HSV-1, HSV-2, VZV, CMV, EBV, and HHV-6. The performance of specific IgG CSF/serum antibody index (AI) was not satisfactory. Conclusion Our study indicates that the multiplex PCR method is the most suitable for the diagnosis of CNS infections caused by HHVs. However, due to the high cost of the PCR method, the positive results of the specific AI may be significant, but the negative results are unreliable, especially in limited health care facilities.Öğe The presence of Torque teno virus in chronic obstructive pulmonary disease(E-Century Publishing Corp, 2014) Feyzioglu, Bahadir; Teke, Turgut; Ozdemir, Mehmet; Karaibrahimoglu, Adnan; Dogan, Metin; Yavsan, MehmetTorque Teno Virus (TTV) has been identified as transfusion-transmitted virus in humans, initially. Although TTV viremia is extremely common in the general population worldwide, there is no direct causal evidence linking TTV infection to specific clinical manifestations. Our hypothesis was that TTV might play a role in Chronic obstructive pulmonary disease (COPD) by inducing inflammatory mechanisms previously identified. The study was conducted on 57 COPD patients and 39 healthy control groups. COPD patient groups included: the patients (n:20) with exacerbation needed noninvasive ventilation, the patients (n: 19) who received only medical treatment, and the invited patients (n: 18) for outpatient control. Serum samples were collected from patients and voluntary blood donors. TTV DNA quantification was carried out with a real time PCR by the hybridization probe system and viral load was interpreted through the crossing point value. TTV DNA was detected in the majority of both patients and healthy control groups. The prevalence was 94.4% (17/18) in patients for outpatient control, 94.7% (18/19) in patients who received only medical treatment, 100% (20/20) in patients with exacerbation needed noninvasive ventilation and 84.6% (33/39) in healthy controls. This difference was not statistically significant. However, CP values was statistically different in all the patient groups from the control group. TTV DNA prevalence was higher in patients than healthy individuals. More interesting thing, viral load was highest in the patients with exacerbation needed noninvasive ventilation. As a result, TTV may be associated with COPD and the severity of it.Öğe Rapid Dissemination of Multidrug Resistant Providencia Stuartii- A University Hospital Based Study(Allied Acad, 2014) Feyzioglu, Bahadir; Gueldemir, Dilek; Karagoz, Alper; Erayman, Ibrahim; Demircili, Mehmet Emin; Baykan, MahmutProvidencia stuartii infections are not common. There are a few reports of declared outbreaks or spreads. In our study, nosocomial dissemination by extended-spectrum beta-lactamase (ESBL) producing Providencia stuartii in a variety of clinics was investigated in a short period. Nine multidrug-resistant Providencia stuartii clinical isolates were collected between October and November in 2011. Nine isolates were obtained from six patients who had been hospitalized in a variety of clinics at a university hospital in Turkey. The clonal relationship among nine isolates was determined by pulsed-field gel electrophoresis. All isolates were ESBL positive and resistant to quinolones, and we determined amikacin resistance to three strains. There were two antibiotypes, and a second PFGE profile was not observed in different types. The outbreak was due to the dissemination of one epidemic multiresistant clone of Providencia stuartii. We considered that it had been a common dissemination from a single source and the first isolate which was collected from the first patient was epidemic clone. The respiratory tract colonization of the first patient was considered to be the possible route of transmission. The resistance to amikacin occurred during treatment with aminoglycoside. It was noteworthy.Öğe Recurrent meningitis by Streptococcus pneumoniae in a girl with cochlear implant and head trauma despite 13-valent conjugated pneumococcal vaccine(Soc Argentina Pediatria, 2019) Emiroglu, Melike; Alkan, Gulsum; Feyzioglu, Bahadir; Aycan, Ahmet E.; Ceyhan, MehmetRecurrent bacterial meningitis is a very rare phenomenon in children. Skull base fractures and cochlear implant are the important predisposing factors and, Streptococcus pneumoniae is the most frequently isolated agent. Implementation of 13-valent conjugated pneumococcal vaccine (PCV13) has reduced the occurence of invasive pneumococcal diseases. Vaccination breakthrough is typically related to underlying predisposing conditions. Herein, we reported recurrent pneumococcal meningitis in a patient with a cochlear implant who experienced a head trauma after being fully vaccinated with PCV13. The patient experienced three meningitis episodes within one year. S. pneumoniae was determined on CSF culture in the first and third episodes and detected by PCR at the second episode. Neurosurgical intervention was performed after the third meningitis episode, and the patient had no recurrence problems for the following two years. To our knowledge, breakthrough S. pneumoniae serotype 1 meningitis after full PCV13 immunization has not been reported elsewhere in the literature.Öğe The Role of New Technologies for Diagnosis of Coronavirus Disease 2019 in Near Future(Georg Thieme Verlag Kg, 2020) Feyzioglu, Bahadir; Ozdemir, Mehmet[Abstract Not Availabe]Öğe The role of torque teno virus (TTV) viremia in sarcoidosis etiology(Kuwait Medical Assoc, 2019) Teke, Turgut; Feyzioglu, Bahadir; Tosun, Mustafa; Dogan, Metin; Yesildag, Kerim; Ozdemir, MehmetObjective: To investigate a possible role of torque teno virus (TTV) in the etiology of sarcoidosis Design: Controlled prospective study Setting: Faculty of Meram Medicine, Necmettin Erbakan University, Konya, Turkey Subjects: This cross-sectional, clinical study included 37 sarcoidosis patients and 9 healthy control patients. Intervention: TTV DNA quantification was performed by real-time PCR by the hybridization probe system, and viral load was interpreted by means of the crossing point (CP) value in sera of subjects. Main outcome measures: The prevalence and viral load of TTV Results: TTV DNA was detected in the majority of both patients and healthy controls. The prevalence was 59.5% in patients and 55.6% in controls. This difference was not statistically significant. However, CP values were statistically different in the patient group from the control group (p < 0.001); namely, the viral load of TTV was higher in the patient group than in the healthy control group. Conclusions: Overall, these findings demonstrate for the first time that TTV plays a role in the etiology of sarcoidosis. It is likely that the role of TTV in the pathogenesis of sarcoidosis is an aberrant immune response, driven by viral antigens.
