Yazar "Gurbilek, Mehmet" seçeneğine göre listele

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    Are dietary and serum advanced glycation end-products related to inflammation and oxidation biomarkers in breast cancer patients: a follow-up study
    (Springer, 2023) Alkan, Senay Burcin; Artac, Mehmet; Aksoy, Faruk; Belviranli, Mehmet Metin; Gurbilek, Mehmet; Cizmecioglu, Hilal Akay; Rakicioglu, Neslisah
    PurposeThis study is aimed at evaluating the relationship between dietary and serum advanced glycation end-products (AGEs) with serum inflammatory and oxidative stress biomarkers in breast cancer (BC).MethodsA sample of BC patients was followed for 12 months (March 2020-January 2022). Three-day food consumption record and serum samples were taken before surgery (T1), before chemotherapy (T2), at the 6(th) month of chemotherapy (T3), and at the 12(th) month of chemotherapy (T4). Dietary AGE intake was represented by carboxymethyl lysine (dCML). Serum levels of CML, inflammation, and oxidation biomarkers were determined with biochemical blood tests. The results were compared according to human epidermal growth factor receptor-2 (HER2) status.ResultsThirty-two women with BC and 32 age and body mass index-matched healthy women participated. No significant correlation was found between dCML and serum CML, inflammatory or oxidative stress biomarkers at T1, T2, and T4. A weak positive correlation was demonstrated between dCML and serum malondialdehyde levels (rho=0.355, p=0.046) at T3. The serum CML, inflammation, and oxidation biomarker levels of the HER2- group were significantly higher than those of the HER2+ group at T1.ConclusionThis study suggests that there is limited correlation between dCML and serum inflammation and oxidative stress biomarkers in BC patients. Inflammation and oxidative biomarker levels appear to decline with treatment although dietary and serum AGE levels show not a corresponding significant decline. The HER2- subtype appears to be associated with higher dietary and serum AGEs and higher inflammatory and oxidative stress biomarkers.
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    Can Urinary KIM-1 and NGAL Predict Management Endoscopic Surgery in Acute Unilateral Obstructive Stone Disease? Results from a Prospective Cohort Study
    (Karger, 2021) Goger, Yunus Emre; Ozkent, Mehmet Serkan; Topcu, Cemile; Atici, Ahmet; Sonmez, Mehmet Giray; Balasar, Mehmet; Gurbilek, Mehmet
    Introduction: Kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) are the leading novel biomarkers used efficiently in acute kidney injury (AKI). The levels of these biomarkers increase especially in the early period of nephrotoxic and ischemic renal damage. In this study, we aimed to investigate the clinical importance of NGAL and KIM-1 biomarkers used in the effective evaluation of kidney functions in patients with acute unilateral obstructive stone disease (AUOSD) in the management of endoscopic surgery. Materials and Methods: We prospectively included patients who underwent endoscopic surgery due to AUOSD between January 2018 and December 2019. Urine KIM-1 and NGAL values of the patients were measured preoperative period, postoperative 4th h, and postoperative 7th day. The patients were evaluated according to the location and size of the stone, the degree of renal hydronephrosis, the duration of the operation, complications, and JJ stent placement. Results: The study enrolled 50 patients. Urinary KIM-1/Cr and urinary NGAL/Cr ratios were higher in postoperative 4th h than in others (p < 0.001). Also, we found that urinary KIM-1/Cr and urinary NGAL/Cr ratios with Grade 2 and higher hydronephrosis were statistically higher than Grade 0-1 hydronephrosis (p < 0.001 and p: 0.042, respectively). Additionally, a preoperative urinary KIM-1 value of 1.24 ng/mL had a sensitivity of 78% and a specificity of 63% to predict the presence of hydronephrosis. When urine KIM-1 and NGAL results were compared with surgery time, stone size and location, serum creatinine (sCr) value, and Post-Ureteroscopic Lesion Scale grade, the difference was not statistically significant. Postoperative 7th day NGAL/Cr and KIM-1/Cr ratios were statistically higher in those with JJ stents placed (p: 0.03 and p: 0.004, respectively). Conclusion: KIM-1 and NGAL can be used in our assessment of renal function in patients with AUOSD, even if sCr is normal. Also, these biomarkers can predict the presence of hydronephrosis. It can be helpful in determining the time of surgical treatment, as well as providing information in the follow-up of patients with JJ stents after treatment.
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    The effects of trans-9 18:1 octadecenoic acid isomer on levels of sICAM-1, sVICAM-1 and IGF-1
    (Walter De Gruyter Gmbh, 2011) Akoz, Mehmet; Kocabas, Rahim; Topcu, Cemile; Gurbilek, Mehmet
    Objective: The effects of trans-9 18: 1 octadecenoic acid isomer from the fatty acid isomers taken with diet on soluble cell adhesion molecules (sICAM-1: Soluble intercellular adhesion molecule-1 and sVCAM-1: Soluble vascular cell adhesion molecule-1) and insulin like growth factor-1 (IGF-1) levels were investigated on rats. Method: The rats which were fed for two weeks by the same diet were separated into experimental and control groups. Fifty mg/day trans-9 18: 1 octadecenoic acid isomer was added to the diet of experimental group for 10 days. Following the completion of special diet, fatty acid composition in both groups was studied by gas chromatography analysis. Results: Trans-9 18: 1 fatty acid level in the experimental group was higher than control group (p<0.01). The soluble cell adhesion molecules (sICAM-1 and sVCAM-1) increased (p<0.01) and IGF-1 levels decreased (p<0.05) in rats fed with trans-9 18: 1 octadecenoic acid. Conclusion: Our results indicate that, high levels of dietary trans fatty acid isomers are a factor in the increasing of cell adhesion molecules (sICAM-1, sVCAM-1) levels and decreasing of IGF-1 levels. Hence, measurement of these parameters may be beneficial in patients with coronary artery disease and atherosclerosis, as well as in other pathological cases. We believe that these parameters may also be useful for early diagnosis.
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    The Evaluation of Dietary Antioxidant Capacity, Dietary Inflammatory Index and Serum Biomarkers in Breast Cancer: A Prospective Study
    (Routledge Journals, Taylor & Francis Ltd, 2023) Alkan, Senay Burcin; Artac, Mehmet; Aksoy, Faruk; Belviranli, Mehmet Metin; Gurbilek, Mehmet; Cizmecioglu, Hilal Akay; Rakicioglu, Neslisah
    Dietary antioxidant capacity (dTAC) and dietary inflammatory index (DII) are commonly used to assess nutrition. This prospective study examined dTAC, DII, and serum biomarkers in women with breast cancer (BC). Patients were followed-up before surgery (T-1), before chemotherapy (T-2), at 6(th) (T-3) and 12(th) months of chemotherapy (T-4). Serum tumor necrosis factor alpha, interleukin 1 & beta;, interleukin 6, protein carbonyl, malondialdehyde, total antioxidant capacity (TAC), and total oxidant status levels were analyzed. Dietary antioxidant intake, dTAC, and DII were determined using a three-day dietary record. dTAC was calculated using vitamin C equivalent (VCE), oxygen radical absorption capacity (ORAC), trolox equivalent antioxidant capacity (TEAC), total radical-trapping antioxidant parameter (TRAP), and ferrous ion reducing antioxidant potential (FRAP). This study included 32 women with BC and 32 controls (CG). ORAC, TEAC, TRAP, and FRAP were significantly lower in BC than in CG. During follow-up, only ORAC increased significantly at T-2 compared to T-1. A weak positive correlation was found between dTAC (VCE) and serum TAC levels at T-2 (rho = 0.371, p = 0.036). The relationship between diet and serum biomarkers was not significant. Multicenter prospective studies on different age groups are needed to understand the association between diet and serum biomarkers levels in patients with BC.
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    Evaluation of the radioprotective effects of thymoquinone on dynamic thiol-disulphide homeostasis during total-body irradiation in rats
    (Oxford Univ Press, 2019) Deniz, Cigdem Damla; Aktan, Meryem; Erel, Ozcan; Gurbilek, Mehmet; Koc, Mehmet
    Ionizing radiation-induced free radicals cause functional and structural harmful effects. Thiol, an important antioxidant, plays a major role in the eradication of reactive oxygen molecules. Thiol/disulphide homeostasis is a marker of oxidative stress. The objective of this study was to assess the potential radioprotective effects of thymoquinone (TQ) on the dynamic thiol/disulphide homeostasis of rats receiving total-body irradiation (IR). Twenty-two rats were divided into three groups to test the radioprotective effectiveness of TQ. The sham control group did not receive TQ or IR. The IR group received only total-body IR. The TQ + IR group received IR plus TQ. Following IR, blood samples were taken. The thiol/disulphide homeostasis parameters were analysed by a newly established method. In the IR group, native thiol and the native thiol/total thiol ratio were significantly decreased (P = 0.003 and P = 0.003, respectively), whereas the disulphide/native thiol and disulphide/total thiol ratios were significantly increased when compared with those of the sham control group (P = 0.003 and P = 0.003, respectively). In the TQ + IR group, the mean disulphide, native thiol and total thiol levels and the disulphide/native thiol, disulphide/total thiol and native thiol/total thiol ratios were not found to be significantly different when compared with those of the sham control group (P > 0.05 for all). Thiol/disulphide homeostasis was found to be disturbed after IR exposure. The results showed that TQ had antioxidant effects and reduced the IR-induced oxidative stress, which was demonstrated through the dynamic thiol/disulphide homeostasis. Thus, the use of TQ before radiation treatment helped protect the rats from oxidant side effects.
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    Evaluation of the relationship of erythrocyte membrane Na plus /K plus -ATPase enzyme activity and tumor response to chemoradiotherapy in patients diagnosed with locally advanced nonsmall cell lung cancer and glioblastoma multiforme
    (Wolters Kluwer Medknow Publications, 2018) Cetinkaya, Cigdem Damla; Gurbilek, Mehmet; Koc, Mehmet
    Context: Radiotherapy is the commonly used therapeutic modality for inoperable cancer types. We investigated chemoradiotherapy (CRT) effects on the Na+/K+-ATPase enzyme. Aims: The aim of the present study was to determine the usefulness of Na+/K+-ATPase enzyme as a prognostic factor and as a potential target for increasing the CRT response of nonsmall cell lung cancer (NSCLC) and glioblastoma multiforme (GBM). Settings and Design: We prospectively evaluated 30 patients (all were treated with CRT) and 20 healthy controls. Subjects and Methods: Blood samples were taken before and after the completion of CRT from the patients and once from the control group. Erythrocyte membranes were isolated and Na+/K+-ATPase enzyme activities were measured. Statistical Analysis Used: The statistical significance was calculated using the one-way analysis of variance test and the Tukey's test. Results: Na+/K+-ATPase activity levels were increased in the patient groups before completion of CRT CRT, when compared to the control group. A significant decrease in Na+/K+-ATPase activity was noted in the patient groups after the completion of CRT when compared to before CRT, but the activity remained higher than in the control group. No relationship was noted between survival and Na+/K+-ATPase activity in NSCLC and GBM patients. Conclusion: Levels of Na+/K+-ATPase activity were initially high in patients with NSCLC and GBM, and decreased after the completion of CRT. This supports a linkage between the altered activity of Na+/K+-ATPase and the treatment effects of CRT. The observed change in Na+/K+-ATPase activity in cancer patients receiving CRT suggests that targeting this enzyme could represent a novel mean of combatting NSCLC and GBM.
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    Urinary kidney injury molecule-1 levels in renal stone patients
    (Springer, 2016) Balasar, Mehmet; Piskin, Mehmet Mesut; Topcu, Cemile; Demir, Lutfi Saltuk; Gurbilek, Mehmet; Kandemir, Abdulkadir; Ozturk, Ahmet
    To study kidney injury molecule-1 (KIM-1) biomarker levels, indicating renal tubular damage, in patients with kidney stones and in those who underwent minimally invasive method stone treatment. Sixty patients with renal stones between 10 and 20 mm were included into the present study. Patients who were divided into three cohorts underwent micropercutaneous nephrolithotomy (microperc), retrograde intrarenal stone surgery (RIRS), and percutaneous nephrolithotomy (PNL). Urine samples were obtained from all participants before, 4 h and 14 days after the procedure. In all the samples obtained, urinary KIM-1 and creatinine (Cr) levels were measured and KIM-1/Cr ratios (ng/mg creatinine) were calculated. Preoperative urine KIM-1/Cr ratio was higher than postoperative 14th day. The bigger the renal stone size, the higher was the ratio (correlation coefficient 0.353, p = 0.006). According to preferred treatment procedure, there was a statistically significant decrease in preoperative and postoperative 4th hour and 14th day urine KIM-1/Cr rates in the RIRS and PNL, yet none in the microperc group (p = 0.010, p = 0.001, p = 0.212, respectively). In renal stone patients, the elevated urine KIM-1/Cr ratio levels increase further according to stone size. KIM-1/Cr ratio is a promising marker might be helpful in monitoring the damage created by stone disease.
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    Vitamin E partially ameliorates cyclophosphamide-induced nephrotoxicity in rats
    (Mattioli 1885, 2016) Cuce, Gokhan; Esen, Haci Hasan; Koc, Tugba; Canbaz, Halime Tuba; Limandal, Cisem; Kalkan, Serpil; Gurbilek, Mehmet
    Purpose: Cyclophosphamide (CP) is a widely used anti chemotherapeutic drug, which causes nephrotoxicity due to its toxic metabolites. This study was carried out to assess the effects of vitamin E on cyclophosphamide induced renal toxicity in rats. Model: Twenty-eight Wistar albino rats were assigned to four groups, which were given 20 mg/kg CP, 20 mg/kg CP + 100 mg/kg vitamin E, 100 mg/kg vitamin E, or 20 mg/kg isotonic sodium chloride solution intraperitoneally each day for 7 days. Effects were assessed by histology of the kidney, TUNEL assay and measurement of serum uric acid and creatinine. Results: Cyclophosphamide significantly increased glomerular inflammation, edema, congestion and tubular degeneration, TUNEL positive cells, while addition of vitamin E significantly decreased glomerular inflammation, edema and TUNEL positive cells. Cyclophosphamide did not affect urea and creatinine levels, which may due to the absence of renal necrosis. Conclusion: Vitamin E application appears to partially ameliorate Cyclophosphamide induced renal toxicity.