Yazar "Gurbuz, Ali Sami" seçeneğine göre listele

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    Comparison of embryo morphokinetics following intracytoplasmic sperm injection in smoker and non-smoker couples: Are the results different?
    (Pakistan Medical Assoc, 2017) Salvarci, Ahmet; Gurbuz, Ali Sami; Uzman, Sukru; Kaya, Melek; Gorkemli, Huseyin
    Objective: To assess early embryo development via time-lapse in smokers and non-smokers. Methods: The retrospective study was conducted at Novafertil IVF centers in Konya, Turkey and comprised oocytes of both smoker and non-smoker couples subjected to in vitro fertilisation /introcytoplasmic sperm injection from 2012 to 2015. Age, basal follicle-stimulating hormone, number of stimulation days, amount of gonadotropin used, number of metaphase II oocytes, number of embryos transferred and pregnancy, abortus and clinical pregnancy rates were noted. The embryos were observed for 72 hours in the time-lapse monitoring system. SPSS 22 was used for data analysis. Results: Of the 257 couples, 132(51.4%) were non-smokers and 125(48.6%) were smokers. A total of 1,414 oocytes were collected from non-smokers and 1,280 oocytes from smokers. There was no significant difference in the age of patients and number of stimulation days between the smoker and non-smoker groups (p>0.05). The number of oocytes, fertilised oocytes, transferred embryos and metaphase II oocytes was significantly less in the smoker group (p<0.05). The rate of pregnancy and ongoing pregnancy was also lower in the smoker group (p<0.05)smoker and non-smoker groups (p<0.05). A prolongation was observed in time to pronuclear fading. A difference was observed in time of pronuclei appearance, t8, t9+ cleavage times in time-lapse in the and t2 cleavage times in time-lapse in the non-smoker group (p<0.05). Some chromosomal number and structural defects were identified in preimplantation genetic in some embryos with prolongation in time-lapse cleavage time in the smoker and nonsmoker groups. Conclusion: The negative impacts of smoking were not observed at each cleavage phase of embryo development in time-lapse.
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    Evaluation from a different perspective of 10-year results of infertile males with Y chromosome AZFc microdeletions compared with a control group
    (Wiley, 2020) Salvarci, Ahmet; Gurbuz, Ali Sami; Balasar, Mehmet
    AZFc microdeletions will be evaluated upon being divided into partial and complete subgroups. The association of deletions with reactive oxidative stress (ROS) and sperm DNA fragmentation (SDFI) and the impact of their coexistence on fertility starting from the pregnancy process until live birth will be presented. Semen analyses, microbiological results, hormones, ROS and sperm TUNEL tests were checked. Preimplantation genetic testing (PGT) was planned for relevant patients. Intracytoplasmic sperm injection (ICSI) was applied. Their embryo fragmentation was monitored via time lapse. Their results were compared with those with no AZF deletion and no other genetic problems. Azoospermia rate was 71.5%, m-TESE success rate was 25%, pregnancy rate was 26% and live child rate was 2.2%. No difference was detected between the partial and total groups in terms of ROS and SDFI rates and no difference was identified with the control group. Better results were obtained in terms of live child rate in patients with partial AZFc and low ROS/SDFI. Spermatozoon was retrieved in AZFc deletions and pregnancy, and live child was identified. No AZFc impact was observed on ROS and SDFI in the results compared with the control groups in terms of their coexistence.
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    GnRH agonist triggering affects the kinetics of embryo development: a comparative study
    (Biomed Central Ltd, 2016) Gurbuz, Ali Sami; Gode, Funda; Uzman, Mehmet Sukru; Ince, Betul; Kaya, Melek; Ozcimen, Necati; Ozcimen, Emel Ebru
    Background: To evaluate the effects of an ovulation triggering agent, human chorionic gonadotropin (hCG), versus a gonadotropin-releasing hormone agonist (GnRHa) on early embryo development in vitro using a time-lapse system. Methods: Retrospective analysis of a prospectively collected database. A total of 739 embryos from 152 infertile couples undergoing intracytoplasmic sperm injection cycles. Interventions : Embryo culture in a time-lapse incubator (EmbryoScope, Vitrolife, Goteborg, Sweden). Main Outcome Measures: Embryo morphokinetic parameters. Results: In the 152 women, 252 embryos were derived from GnRHa-triggered cycles compared with 487 embryos derived from hCG-triggered cycles. Time-lapse analysis revealed that embryos from cycles triggered by a GnRHa cleaved faster than embryos derived from hCG-triggered cycles. Conclusion: Triggering with a GnRHa in in vitro fertilization cycles affects embryo kinetics.
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    GnRH agonist triggering affects the kinetics of embryo development: a comparative study
    (Biomed Central Ltd, 2016) Gurbuz, Ali Sami; Gode, Funda; Uzman, Mehmet Sukru; Ince, Betul; Kaya, Melek; Ozcimen, Necati; Ozcimen, Emel Ebru
    Background: To evaluate the effects of an ovulation triggering agent, human chorionic gonadotropin (hCG), versus a gonadotropin-releasing hormone agonist (GnRHa) on early embryo development in vitro using a time-lapse system. Methods: Retrospective analysis of a prospectively collected database. A total of 739 embryos from 152 infertile couples undergoing intracytoplasmic sperm injection cycles. Interventions : Embryo culture in a time-lapse incubator (EmbryoScope, Vitrolife, Goteborg, Sweden). Main Outcome Measures: Embryo morphokinetic parameters. Results: In the 152 women, 252 embryos were derived from GnRHa-triggered cycles compared with 487 embryos derived from hCG-triggered cycles. Time-lapse analysis revealed that embryos from cycles triggered by a GnRHa cleaved faster than embryos derived from hCG-triggered cycles. Conclusion: Triggering with a GnRHa in in vitro fertilization cycles affects embryo kinetics.
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    Severe ovarian hyperstimulation syndrome and gonadotropin-releasing hormone agonist trigger in patients with hypogonadotropic hypogonadism: A report of two cases
    (Galenos Yayincilik, 2020) Gurbuz, Ali Sami; Gode, Funda; Kilic, Fatma; Gurbuz, Zeynep Umay; Deveer, Ruya
    Ovarian Hyperstimulation syndrome (OHSS) is a rare condition in patients with hypogonadotropic hypogonadism. Two patients with hypogonadotropic hypogonadism are reported, a rare case of severe OHSS and a case of prevented OHSS via gonadotropin-releasing hormone (GnRH) agonist trigger, respectively. The first case was a 31-year-old patient. in vitro fertilization (IVF) treatment was performed three times but the patient never developed OHSS. The first patient was diagnosed as having severe OHSS on the ninth day after the fresh embryo transfer. She stayed 66 days in hospital and 50.5 litres of fluid were aspirated from her abdomen. The second case was a 26-year-old and primary infertile patient. She had never undergone IVF treatment. The GnRH agonist stimulation test was performed before IVF treatment. After the ovarian stimulation, GnRH agonist trigger was given. Thirty-two oocytes were retrieved from the ovaries and OHSS did not occur. Although severe OHSS is rare, it can develop in patients hypogonadotropic hypogonadism. If a GnRH stimulation test is performed before ovarian stimulation, OHSS can be prevented because the test allows agonist triggering instead of hCG in hypogonadotropic hypogonadism.
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    Ten-year outcomes of patients who developed persistent azoospermia following chemotherapy associated with different oncological diagnoses: A retrospective cohort study from a different perspective
    (Tubitak Scientific & Technological Research Council Turkey, 2022) Salvarci, Ahmet; Gurbuz, Ali Sami; Balasar, Mehmet
    Background/aim: This study evaluated the treatment procedures for chemotherapy (CT)-induced persistent azoospermia and their outcomes from a different perspective. Materials and methods: In 63 patients (mean age: 30.16 +/- 4.91 years) who had undergone CT 11 +/- 5 years earlier, the semen volume, gonadotropins level, FSH level, genetics, micro-testicular sperm extraction (m-TESE) result, sperm DNA fragmentation index (SDFI), semen reactive oxidative stress (ROS) rate, duration of embryonic development, and pregnancy and baby take-home rates were examined. The correlations between the ROS rates and the SDFIs, m-TESE results, sperm motility, pathology scores, time-lapses, and baby take-home rates were evaluated. Results: The semen volumes were 3.5 +/- 1.1/ml. The FSH level following CT was 17.87 +/- 5.80 mIU/ml. A sperm rate of 34.9% was found from the m-TESE result. The mean SDFI and ROS rate were 4 (<15-30>) and 1.29 +/- 0.51, respectively. The time-lapse was calculated as 5h. Pregnancy and live birth were achieved at 20.63% and 12.7%, respectively. In the patients with a low ROS (<= 1.42) and SDFI (<= 15), the m-TESE success rate was high, the FSH value was low, the pathological score and fertilization rate were elevated, the embryonic cleavage period was normal, and the pregnancy and baby take-home rates were high. Conclusion: The sperms may be detected using m-TESE in patients who develop persistent azoospermia associated with CT due to different oncological diagnoses. Our study revealed that a low FSH value and normal ejaculatory ROS rates are positive predictive factors of sperm detection before m-TESE. The motility of the sperms detected after m-TESE and normal SDFI rates were found to be positive predictive criteria of high fertilization, good embryonic cleavage, pregnancy, and live birth.