Yazar "Hitit, Mustafa" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    The effect of medicarpin on PTEN/AKT signal pathway in head and neck squamous cell carcinoma
    (Wolters Kluwer Medknow Publications, 2022) Yigin, Aysel Kalayci; Donmez, Huseyin; Hitit, Mustafa; Seven, Sena; Eser, Nadire; Kurar, Ercan; Seven, Mehmet
    Background/Aim: We aimed to investigate the in vitro modulating effects of medicarpin on the PI3K/AKT signal pathway gene expressions in head and neck squamous cell carcinoma (HNSCC). Materials and Methods: The effect of medicarpin on PTEN and other associated genes in the PTEN/AKT signal pathway was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, real-time quantitative polymerase chain reaction, and Western blot analysis in the SCCL-MT1 (HNSCC) and control (HEK-293) cell lines. Results: The IC50 dose was 80 mu M as a result of medicarpin treatment on HNSCC cells (P = 0.0006). It was found that PTEN and AKT gene expressions increased after the medicarpin administration while PDK1 gene expression was decreased in SCCL-MT1 cells (P = 0.0002, P = 0.0003, and P = 0.05, respectively). Protein expression results showed that medicarpin-treated cells significantly increased in pAKT (P = 0.024), pPTEN (P = 0.032), and decreased pPDK1 (P = 0.059) in SCCL-MT1. Conclusions: Our data show that medicarpin modulates HNSCC cells by increasing the PTEN and decreasing PDK1 expressions. PDK1 gene expression effects mTOR pathway which may increase AKT gene. Our study suggests that both medicarpin extracts combination with the HNSCC drug may be more effective in cancer treatment. Future prospective studies that integrate molecular and pharmacogenetic studies are crucial for revealing the mechanism and preventive medical efforts.
  • Küçük Resim Yok
    Öğe
    Investigation of interferon-tau stimulated genes (ISGs) simultaneously in the endometrium, corpus luteum (CL) and peripheral blood leukocytes (PBLs) in the preluteolytic stage of early pregnancy in ewes
    (Elsevier, 2016) Kiyma, Zekeriya; Kose, Mehmet; Atli, Mehmet Osman; Ozel, Caglayan; Hitit, Mustafa; Sen, Gonca; Kaya, Muhammet
    The aim of the present study was to investigate the expression profiles of Interferon-tau (IFN-T) stimulated genes (ISGs) at the mid-luteal stage of the cycle (on day 13) in the uterine endometrium and extra-uterine tissues, such as the corpus luteum (CL), and in the peripheral blood leukocytes (PBLs) of pregnant and non -pregnant ewes, The objective was to evaluate the possibility of using the regulation of ISG expression in PBLs as a possible early pregnancy indicator in ruminants. For this purpose, multiparous ewes were synchronized and either allowed to mate (pregnancy group) or detected in estrus (cyclic group; day 0). The ewes were slaughtered on day 13, and the PBLs, endometrium and luteal tissues were collected. Total RNA was isolated from eight cyclic and eight pregnant ewes, and qPCR was employed to detect the steady state levels of Interferon -stimulated gene 15 (ISG15), Myxovirus (influenza virus) resistance 1 (Mxl) and Receptor transporter protein 4 (RTP4) mRNAs. The expressions of ISG15, Mxl and RTP4 were detected in the endometrium, CL and PBLs on day 13 of the estrous cycle and pregnancy. The expressions of these ISGs were upregulated only in the endometrium of pregnant ewes compared to non-pregnant ewes, but this stimulation was not observed in the CL and PBLs. The results suggest that the embryo stimulates ISGs only in the endometrium, and the effects are not evident in the extra-uterine tissues on day 13 of pregnancy. This study suggests that the measurement of the ISG expression in the PBLs is not a reliable detection method of early pregnancy in ewes, which are in the preluteolytic stage of early pregnancy. (C) 2016 Elsevier B.V. All rights reserved.
  • Küçük Resim
    Öğe
    MikroRNA Biyogenezi
    (2015) Hitit, Mustafa; Kurar, Ercan; Güzeloğlu, Aydın
    Öz: MikroRNAlar (miRNA) kodlama yapmayan 21-24 nükleotid uzunluğunda RNA molekülleridir. Genel olarak translasyonun baskılanmasına veya mRNAnın yıkımlanmasına neden olurlar. MikroRNA ilk keşfedildiğinde solucanlarda olağandışı spesifik gen ekspresyon mekanizması olarak düşünülmesine rağmen, artık günümüzde ökaryotlarda önemli gen ekspresyon düzenleyicisi olarak kabul edilmektedir. MikroRNA biyogenezi çekirdekte RNA polimeraz II aracılığında transkripsiyon ile başlar ve hairpin yapısında olgun miRNA dizisini içeren uzun miRNA (pri-miRNA)dan oluşur. Hairpin yapısı Drosha (RNAaz III enzimi) ve kofaktörü DiGeorge kritik sendrom bölgesi 8 (DGCR8)den oluşan mikroprosesör tarafından kesilir. Oluşan prekürsör miRNA (pre-miRNA) nükleustan Exportin-5 ile sitoplazmaya taşınır ve diğer RNAaz III enzimi olan Dicer tarafından 21-24 nükleotid uzunluğundaki dubleks miRNAya kesilir. Olgun diziye kesilecek olan iplik miRNA, RNA indüklenmiş susturma kompleksinde (RISC) Argonautea yüklenir. MikroRNAnın 2-8 nükleotidlik çekirdek dizisi hedef mRNA ile tam olarak eşlendiğinde mRNAnın destabilizasyonu sağlanır. Ancak tam olarak eşlenmediği zaman translasyonal baskılanmaya neden olur. MikroRNAların gelişim, farklılaşma ve diğer fizyolojik fonksiyonlarda önemli rol aldığı gösterilmesine rağmen, düzensiz ifadesi durumunda farklı patolojik olaylar ile ilişkilendirilmiştir. MikroRNA biyogenezinin farklı fizyolojik süreçlerde ve hastalıklarda epigenetik etkisinin moleküler düzeyde anlaşılmasının potansiyel önemi bulunmaktadır.