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Öğe Facile microwave-mediated green synthesis of non-toxic copper nanoparticles using Citrus sinensis aqueous fruit extract and their antibacterial potentials(Elsevier, 2021) Jahan, Israt; Erci, Fatih; Isildak, IbrahimThis study focuses on the utilization of orange (Citrus sinensis) fruit extract for microwave mediated green synthesis of copper nanoparticles for the first time. After completion of the synthesis cycles, the presence of a maximum peak at the wavelength of 560 nm by UV-vis spectroscopy indicated the formation of CuNPs. FTIR spectrum identified the functional biomolecules of the orange juice extract, responsible for the bioreduction, stabilization of copper nanoparticles. Scanning Electron Microscopy (SEM) indicated the presence of globular shaped nano-sized copper particles with smooth surfaces whereas Energy Dispersive X-ray Spectrometry (EDX) confirmed the presence of copper, carbon and oxygen atoms with the amount of 77.68%, 17.55% and 4.77%, respectively. Transmission Electron Microscopy (TEM) illustrated that the CuNPs are morphologically round in shape with the distribution range of 6.93 nm-20.70 nm in diameter. X-ray diffraction (XRD) analysis revealed the presence of face-centered cubic crystalline structures of copper nanoparticle, and Zeta potential analysis confirmed their high stability by showing the strong negative potential value (-25.60 mV). Moreover, these biosynthesized copper nanoparticles displayed their efficiency in controlling the growth of the bacteria, i.e., Escherichia coli and Staphylococcus aureus. The results revealed their non-cytotoxic nature on the L929 mouse fibroblast cell line through XTT cell viability assay. All these studies indicated that CuNPs synthesized by orange extract have the potential to serve as a green nanomaterial for biomedical applications.Öğe GREEN SYNTHESIS OF COPPER OXIDE NANOPARTICLES USING OCIMUM BASILICUM EXTRACT AND THEIR ANTIBACTERIAL ACTIVITY(Parlar Scientific Publications (P S P), 2017) Altikatoglu, Melda; Attar, Azade; Erci, Fatih; Cristache, Corina Marilena; Isildak, IbrahimThe investigation aims the synthesis of copper oxide nanoparticles (CuO NPs) using Ocimum basilicum plant extract at room temperature. This method is completely a green method, free from toxic and harmful solvent. The CuO NPs were sytnhesized by mixing copper sulphate dehydrate (CuSO4.5H20) and aqueous Ocimum basilicum leaf extract. The biosynthesized copper oxide nano-particles were characterized by UV-vis spectro-scopy, Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The existence of the CuO NPs was revealed by UV-vis spectroscopy. The FTIR spectra of control (leaf extract) and synthesized CuO NPs identified the functional groups of the active components. SEM images brought out that the particles were spherical in shape, and the size was found to be ranging under 70 rim. Further, the synthesized CuO NPs were tested for antibacterial activity by standart disc diffusion method. The nanoparticles were found to have antibacterial activity against pathogenic bacterial strains Escherichia coli and Staphylococcus aureus by the observation of inhibition zones around each well.Öğe Green synthesis of silver nanoparticles using Thymbra spicata L. var. spicata (zahter) aqueous leaf extract and evaluation of their morphology-dependent antibacterial and cytotoxic activity(Taylor & Francis Ltd, 2018) Erci, Fatih; Cakir-Koc, Rabia; Isildak, IbrahimSilver (Ag) nanoparticles (NPs) were green synthesized at room temperature using different concentrations of the Thymbra spicata L. var. spicata (Zahter) aqueous leaf extracts for the first time. With the synthesis of AgNPs using the leaf extract of Cynara scolymus (Artichoke) and Mentha piperita (Peppermint), the biological activities of the nanoparticles synthesized using leaf extract of three economically significant plants have been studied comparatively. Nanoparticles were characterized by different spectroscopic and microscopic analysis. TEM analysis of the biosynthesized AgNPs revealed that the size and shape of the AgNPs were changed with the plant extract concentration. Biologically synthesized AgNPs from leaf extracts of the three different plants displayed significant differences in antibacterial activity against two different gram-negative and gram-positive bacteria. Also, the results from this study show the shape dependence of the antibacterial and cytotoxic activity of silver nanoparticles synthesized using T. spicata leaf extract. The nanoparticles with different shapes exhibited the strongest antibacterial and cytotoxic activity compared to mostly spherical nanoparticles. Present results clearly indicate that biological activities of silver nanoparticles were affected by nanoparticle shape and the source of the plant extract used in the synthesis.Öğe Microwave-Assisted Green Synthesis of Non-Cytotoxic Silver Nanoparticles Using the Aqueous Extract of Rosa santana (rose) Petals and Their Antimicrobial Activity(Taylor & Francis Inc, 2019) Jahan, Israt; Erci, Fatih; Isildak, IbrahimGreen methods using biological extracts, in particular plant-based solutions, have shown great potential for silver nanoparticle synthesis. A microwave-assisted single-step phytosynthesis of silver nanoparticles is described in the present study. The aqueous extract obtained from the Rosa santana (rose) petals was used for the first time in the synthesis. The synthesized nanoparticles obtained after optimized microwave conditions for time and temperature were analyzed by ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta-size analysis. The results obtained from the characterization studies showed that the synthesized nanoparticles were nearly spherical in shape with sizes from 6.52 nm to 25.24 nm with an average particle size of 14.48 nm with a face-centered cubic structure. The antibacterial activities of the synthesized nanoparticles were evaluated and revealed that the silver nanoparticles displayed good inhibition against both Gram-negative and Gram-positive bacteria. Also, the cytotoxic effect of the silver nanoparticles on a mouse fibroblast cell line (L929) was studied by a cell viability assay. The results showed that phytosynthesized silver nanoparticles were nontoxic to the healthy normal cell line at all tested concentrations.Öğe Microwave-irradiated green synthesis of metallic silver and copper nanoparticles using fresh ginger (Zingiber officinale) rhizome extract and evaluation of their antibacterial potentials and cytotoxicity(Taylor & Francis Inc, 2021) Jahan, Israt; Erci, Fatih; Cakir-Koc, Rabia; Isildak, IbrahimHerein the microwave-assisted green synthesis of both silver (Ag) and copper (Cu) nanoparticles (NPs) by microwave irradiation have been successfully carried out using fresh ginger rhizome extract. Different analytical methods were used for the characterization of the nanoparticles. The size distribution of AgNPs was found from the range of 2.68 nm to 42.69 nm, whereas for CuNPs the size range was run from 11.32 nm to 33.70 nm in diameter. From the antibacterial assay, we found that both silver and copper nanoparticles demonstrated significant potentials against tested bacterial strains. Besides,in vitrocytotoxic effects of NPs were also tested on the L929 mouse fibroblast cell line through XTT cell viability assay which confirmed the non-toxic nature of AgNPs and CuNPs at all tested concentrations. Thus, the obtained biological properties, suggested the use of rhizome extract ofZingiber officinalemediated non-toxic AgNPs and CuNPs in further biomedical and clinical applications.Öğe Plant extract mediated silver nanoparticles by concentrated sunlight and their antibacterial and cytotoxic activities(Taylor & Francis Inc, 2022) Kutuk, Yagmur; Yontem, Mustafa; Erci, Fatih; Esirgenler, Busra; Isildak, Ibrahim; Totu, Eugenia EftimieHere we report the synthesis of silver nanoparticles (AgNPs) under concentrated sunlight by using a newly designed solar system. In the study, AgNPs synthesized by using different plant extracts of Ginko biloba (Gb), Alpinia officinarum (Ao), Foeniculum vulgare (Fv) and Cassia angustifolia (Ca) were characterized by different techniques. The antibacterial activity of the AgNPs was determined by agar diffusion assay. TEM analysis revealed the spherical nanoparticles with average size of 26, 23,44 and 32 nm in diameter for Gb, Ao, Fv and Ca AgNPs, respectively. Also, we found that the AgNPs were not cytotoxic at the concentration of 2.5 mu g/mL. We also revealed that Ca AgNPs showed the highest antibacterial activity against S. aureus with inhibition zone of 15.27 mm. All in all, the results indicated that the formation of AgNPs was achieved in less than 1 h without any energy consumption in the solar system with concentrated sunlight.Öğe Rapid green synthesis of non-cytotoxic silver nanoparticles using aqueous extracts of 'Golden Delicious' apple pulp and cumin seeds with antibacterial and antioxidant activity(Springer Int Publ Ag, 2021) Jahan, Israt; Erci, Fatih; Isildak, IbrahimA simple, facile and rapid microwave irradiated system was applied to synthesize silver nanoparticles using 'Golden Delicious' apple pulp (Malus domestica) and cumin (Cuminum cyminum) seed extracts. The phytosynthesized AgNPs were characterized by Ultraviolet-Visible Spectroscopy (UV-vis), Fourier transform infrared (FTIR), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM) and Zeta sizer analysis. In the study, the presence of face-centered cubic crystalline structured metallic silver in AgNPs from apple and cumin extracts and the monodisperse nature of AgNPs with the size distribution range of 5.46-20 nm and 1.84-20.57 nm were confirmed, respectively. This study established an efficient green synthesis approach that created so far, the smallest silver nanoparticles by using these two extracts. According to the results obtained, AgNPs synthesized using both extracts were non-toxic against L929 mouse fibroblast cells, while they were effective against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria with a greater effect on S. aureus. Moreover, AgNPs synthesized through cumin extract exhibited a higher ABTS scavenging ability (96.43 +/- 0.78% at 160 mu g/mL) in comparison to apple pulp extract mediated AgNPs, while both AgNPs showed lower activity for DPPH (27.84 +/- 0.56% and 13.12 +/- 0.32% from cumin seed and apple pulp extracts, respectively). In summary, our results suggest the green non-cytotoxic AgNPs synthesized in this study could be a promising template for further biological and clinical applications. [GRAPHICS]
