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    Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D
    (Walter De Gruyter Gmbh, 2022) Kader, Saadet; Akdag, Turan; Ecer, Busra; Abusoglu, Sedat; Unlu, Ali
    Objectives Vitamin D is one of the major hormones involved in the metabolism of calcium (Ca) and phosphorus (P). In the present study, we aimed to determine the analytical performance of the immunoassay method used for determining plasma 25-hydroxyvitamin D [25(OH)D] levels in routine clinical practice in laboratories. Methods Venous blood samples were collected from 156 patients for the comparisons and were analyzed with Siemens ADVIA Centaur XPT, the Roche Cobas 6,000's module e601, Abbott Architect i2000, and the liquid chromatography with tandem mass spectrometry (LC-MS/MS). Results The four methods were analyzed and compared through the Passing-Bablok regression for 25(OH)D, and the highest correlation was found at LC-MS/MS and Cobas 6,000's module e601 (r=0.799), LC-MS/MS/Abbott Architect i2000, and LC-MS/MS/Siemens ADVIA Centaur XPT as r=0.736, 0.721, respectively. The correlation coefficient was found between Abbott Architect i2000 with Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.934 and r=0.907, respectively. Also, the correlation coefficient was found between Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.906. Conclusions The Roche Cobas assay showed better performance, compared with the other assays. Based on our findings, the chemiluminescence methods in automated systems seem to be expedient.
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    Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D
    (Walter De Gruyter Gmbh, 2022) Kader, Saadet; Akdag, Turan; Ecer, Busra; Abusoglu, Sedat; Unlu, Ali
    Objectives Vitamin D is one of the major hormones involved in the metabolism of calcium (Ca) and phosphorus (P). In the present study, we aimed to determine the analytical performance of the immunoassay method used for determining plasma 25-hydroxyvitamin D [25(OH)D] levels in routine clinical practice in laboratories. Methods Venous blood samples were collected from 156 patients for the comparisons and were analyzed with Siemens ADVIA Centaur XPT, the Roche Cobas 6,000's module e601, Abbott Architect i2000, and the liquid chromatography with tandem mass spectrometry (LC-MS/MS). Results The four methods were analyzed and compared through the Passing-Bablok regression for 25(OH)D, and the highest correlation was found at LC-MS/MS and Cobas 6,000's module e601 (r=0.799), LC-MS/MS/Abbott Architect i2000, and LC-MS/MS/Siemens ADVIA Centaur XPT as r=0.736, 0.721, respectively. The correlation coefficient was found between Abbott Architect i2000 with Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.934 and r=0.907, respectively. Also, the correlation coefficient was found between Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.906. Conclusions The Roche Cobas assay showed better performance, compared with the other assays. Based on our findings, the chemiluminescence methods in automated systems seem to be expedient.
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    Elevated survivin levels in patients with acne vulgaris
    (Wiley, 2022) Aksoy Sarac, Gulhan; Kader, Saadet; Akdag, Turan
    Objective Acne vulgaris is a multifactorial disease of the pilosebaceous unit. As the most common skin disease, it may affect approximately 85% of the young population. Survivin, a member of the inhibitors of the apoptosis (IAP) gene family, can inhibit apoptosis and regulate cell division and proliferation. In the study, we aimed to investigate the potential role of serum survivin in acne vulgaris. Methods Forty individuals who were diagnosed with acne vulgaris and forty healthy subjects as the control group were enrolled in the study. Venous blood samples were collected from each participant, and the serum levels of survivin were measured by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using SPSS software version 25. Results The serum survivin levels were statistically significant between the groups, and the levels of survivin were measured as acne vulgaris patients group 153.44 and control group 104.17 pg/ml, respectively (p < 0.018). When the serum survivin levels were compared according to gender, females had higher levels of survivin than the males (168.16 versus 50.45 pg/mL, p = 0.001). A significant correlation was found between acne severity (p = 0.017) and Scale for Acne Scar severity (SCAR-S) score (p = 0.001) according to the survivin levels. In terms of age, no significant relationship was found between age and survivin (p = 0.4048). Conclusion Elevated serum levels of survivin were determined in acne vulgaris. Moreover, there was a significant correlation between acne stage and SCAR-S score according to survivin levels.
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    Evaluation of BD Vacutainer Eclipse and BD Vacutainer Ultra-Touch butterfly blood collecting sets in laboratory testing
    (Walter De Gruyter Gmbh, 2021) Kader, Saadet; Kirmit, Adnan; Akdag, Turan; Tekindal, Mustafa Agah
    Objectives Choosing the right device for blood collection is an issue that is frequently encountered in laboratory medicine. The purpose of this study was to compare the quality of the samples collected in terms of hemolysis index, pain intensity and tube filling rates of the samples drawn with BD Vacutainer(R) EclipseNeedle and BD Vacutainer Ultra-Touch (TM) Butterfly Blood Collection Set, which is currently used in laboratories. Methods Blood samples were drawn from the 44 apparently healthy adult individuals from different arm sites by routine phlebotomy into Citrate, SST and EDTA tubes in Karapinar State Hospital. K, P, AST, LDH tests and hemolysis index were analyzed by Mindray BS-800 chemistry analyzer. Complete blood count (CBC) was determined by Mindray BC 6000. Results There was no significant difference in K, P, AST, LDH and CBC values between the devices for hemolysis (p>0.05). No significant difference was found out with respect to visual hemolysis and hemolysis index, tube filling speed and pain intensity between the devices. Conclusions Our findings demonstrate that two sets may used for venipuncture blood collection without creating additional hemolysis risk.
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    Evaluation of BD Vacutainer Eclipse and BD Vacutainer Ultra-Touch butterfly blood collecting sets in laboratory testing
    (Walter De Gruyter Gmbh, 2021) Kader, Saadet; Kirmit, Adnan; Akdag, Turan; Tekindal, Mustafa Agah
    Objectives Choosing the right device for blood collection is an issue that is frequently encountered in laboratory medicine. The purpose of this study was to compare the quality of the samples collected in terms of hemolysis index, pain intensity and tube filling rates of the samples drawn with BD Vacutainer(R) EclipseNeedle and BD Vacutainer Ultra-Touch (TM) Butterfly Blood Collection Set, which is currently used in laboratories. Methods Blood samples were drawn from the 44 apparently healthy adult individuals from different arm sites by routine phlebotomy into Citrate, SST and EDTA tubes in Karapinar State Hospital. K, P, AST, LDH tests and hemolysis index were analyzed by Mindray BS-800 chemistry analyzer. Complete blood count (CBC) was determined by Mindray BC 6000. Results There was no significant difference in K, P, AST, LDH and CBC values between the devices for hemolysis (p>0.05). No significant difference was found out with respect to visual hemolysis and hemolysis index, tube filling speed and pain intensity between the devices. Conclusions Our findings demonstrate that two sets may used for venipuncture blood collection without creating additional hemolysis risk.
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    Evaluation of D-Dimer and Neutrophil/Lymphocyte Ratios of COVID-19 Patients Whom Applied to Karapinar State Hospital
    (Marmara Univ, Inst Health Sciences, 2022) Kader, Saadet; Akdag, Turan; Sariyildiz, Levent; Ozel, Zeynep
    Objective: The epidemic which caused by the SARS-CoV-2 virus were defined as COVID-19) and declared as a global pandemic by the World Health Organization (WHO) on March, 2020. Nowadays, many biochemical parameters related to the diagnosis and prognosis of COVID-19 are being investigated. Therefore, we aimed to evaluate D-dimer and neutrophil/lymphocyte ratios (NLR) of COVID-19 patients whom applied to Karapinar State Hospital. Methods: Patients which consisted of 2523, whom diagnosed with COVID-19 between 11 March 2019 and 29 July 2021 at Karapinar State Hospital were included in the study. Age, gender and social history of the patients were recorded. From the results, the relationships between D-dimer and hemogram were evaluated. Results: There was a high correlation between the variables HCT and HGB, PLT and PCT, NEUT# and WBC, and MCH and MCV (r=0.981, r=0.944, r=0.923, r=0.925). In addition, there was a high correlation between RBC and HCT and between RBC and HGB variables (r=0.852, r=0.795). There was a moderate correlation between WBC and MO#, MCHC and MCH, PDW and MPV (r=0.562, r=0.639, r=0.64). All the relationships between these variables were positive, and the value of the correlated parameter increases linearly by unit. Also, the highest positive relationships were between HCT and HGB, PLT and PCT, NEUT# and WBC, MCH and MCV. Moreover, D-dimer and NLR were not correlated (r= - 0.015, p=0.49). Conclusion: In the study, no correlation was observed between D-dimer and neutrophil/lymphocyte ratios of COVID-19 patients. So, more comprehensive and further studies are needed to clarify these results.