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Öğe Anticancer mechanism of Sinapic acid in PC-3 and LNCaP human prostate cancer cell lines(Elsevier, 2018) Eroglu, Canan; Avci, Ebru; Vural, Hasibe; Kurar, ErcanSinapic acid (SA) is a derivative of hydroxycinnamic acid and found in various vegetables and fruit species. Aim was to evaluate the anticancer effects of SA in PC-3 and LNCaP human prostate cancer cells. The effect of SA on cell viability was determined using XTT assay. Expressions of 8 genes for apoptosis and 6 genes for metastasis were evaluated by qPCR. Caspase-3 activity was determined using caspase-3 colorimetric assay kit. Effect of SA on cell invasion was evaluated with cell invasion assay. The IC50 dose of SA in PC-3 and LNCaP cells was found to be 1000 mu M for 72 h. SA treatment increased the expression of BAX, CASP3, CASP8, CYCS, FAS, TIMP-1 and CDH1 however significantly decreased the expression of MMP-9 in PC-3 cells. In LNCaP cells, the expressions of BAX, CASP3, CASP7 and CYCS were significantly elevated; however, a decrease was seen in the expressions of CDH2, MMP-2 and MMP-9 in the SA treatment. Moreover, SA significantly increased caspase-3 activity and suppressed the cell invasion. In conclusion, it is thought that SA has anticancer effect on prostate cancer cells. However, more detailed studies should be conduct to illuminate molecular mechanism of apoptotic and anti metastatic activity of SA.Öğe Apelin triggers macrophage polarization to M2 type in head and neck cancer(Elsevier Gmbh, 2023) Celik, Fatma Secer; Gunes, Canan Eroglu; Yavuz, Emine; Kurar, ErcanCancer comes after cardiovascular diseases in terms of mortality rate in the world. Chemotherapy, radiotherapy and surgical interventions are the current cancer treatment. Recently, it has been observed that immunothera-peutic approaches provide a significant improvement when used along with these interventions. The mono-nuclear system mainly consists of macrophages that play an active role in the pathology of many diseases because of having high plasticity capacities. Previous research suggested that they can be used as an alternative to cancer treatment. Aim was to investigate the effect of apelin on macrophage polarization in the tumor microenvironment.Mouse macrophage cell line RAW264.7 cells and head and were chosen for this study. The apelin expression was knockdown in neck cell carcinoma cell line SCCL MT1 cells using shRNA technique. SCCL MT1 cells having normal or suppressed apelin expression were co-cultured with mouse macrophage RAW264.7 cells. The effect of co-culturing on the expression of inflammatory genes in RAW264.7 cells was investigated.Suppressed apelin expression in SCCL MT1 cells resulted in elevated pro-inflammatory response in co-cultured macrophages. Expression of the IL113, IL6, and TNF alpha genes significantly increased, however anti-inflammatory cytokine levels were significantly decreased. However, in the control group, a downregulation was deter-mined in pro-inflammatory genes, while an increase was observed in anti-inflammatory genes. The protein levels of these cytokines in concordance with the RT-PCR analysis.As a result of this study, apelin released from cancer cells was found to affect macrophage polarization. These results indicated that the apelin peptide may cause the intense presence of M2-type macrophages in the tumor niche, and the therapeutic approaches targeting of apelin in cancer cells may have a potential role in macrophage polarization.Öğe The association between leptin receptor polymorphism and suicidal behaviour in depressed adolescents(Taylor & Francis Ltd, 2020) Acikel, S. Burak; Eroglu, Canan; Dikmen, Asiye Ugras; Kurar, ErcanObjective: Suicide is the second leading cause of death in adolescence. Genetic studies implicate the genetic component of suicide independent from associated psychiatric disorder. Although genetics is an important factor that might be associated with suicide, limited progress is achieved to identify the candidate genes in adolescents. Methods: The study included 97 patients and 106 controls. Five leptin receptor single nucleotide polymorphism (SNP) region examined. Depressive scores were measured with Children Depression Inventory and suicidal behaviour was measured by Suicide Probability Scale. Logistic and linear regression analysis used for determining to predictors. Results: In linear regression analysis (R-2: 0.786) both previous suicide attempt (B:5.553, t:2.613 p: .035) and having a mutant allele in rs1171276 SNP region (B:4.346 t:2.220 p: .048) have been associated with suicidal behaviour. In logistic regression analysis, family history of depression (p < .0001, OR: 4.2 [1.7-9.6]) and number of stressful life events (p: .001, OR: 1.7 [1.3-2.1]) predicted depression significantly. Conclusion: Leptin receptor polymorphism could result in an increase in impulsive behaviour and suicide scores with leptin resistance. Our research is the first study to investigate the relationship between depression, suicidal behaviour and leptin receptor polymorphism in adolescent sample. Similar studies could be carried out on a community basis.Öğe Does Neurokinin 3 Receptor Agonism Affect Hippocampal Neurogenesis in an Experimental Rat Model of Alzheimer's Disease?(Wiley, 2023) Koca, Raviye Ozen; Gormus, Z. Isik Solak; Solak, Hatice; Celik, Fatma Secer; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe The effect of medicarpin on PTEN/AKT signal pathway in head and neck squamous cell carcinoma(Wolters Kluwer Medknow Publications, 2022) Yigin, Aysel Kalayci; Donmez, Huseyin; Hitit, Mustafa; Seven, Sena; Eser, Nadire; Kurar, Ercan; Seven, MehmetBackground/Aim: We aimed to investigate the in vitro modulating effects of medicarpin on the PI3K/AKT signal pathway gene expressions in head and neck squamous cell carcinoma (HNSCC). Materials and Methods: The effect of medicarpin on PTEN and other associated genes in the PTEN/AKT signal pathway was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, real-time quantitative polymerase chain reaction, and Western blot analysis in the SCCL-MT1 (HNSCC) and control (HEK-293) cell lines. Results: The IC50 dose was 80 mu M as a result of medicarpin treatment on HNSCC cells (P = 0.0006). It was found that PTEN and AKT gene expressions increased after the medicarpin administration while PDK1 gene expression was decreased in SCCL-MT1 cells (P = 0.0002, P = 0.0003, and P = 0.05, respectively). Protein expression results showed that medicarpin-treated cells significantly increased in pAKT (P = 0.024), pPTEN (P = 0.032), and decreased pPDK1 (P = 0.059) in SCCL-MT1. Conclusions: Our data show that medicarpin modulates HNSCC cells by increasing the PTEN and decreasing PDK1 expressions. PDK1 gene expression effects mTOR pathway which may increase AKT gene. Our study suggests that both medicarpin extracts combination with the HNSCC drug may be more effective in cancer treatment. Future prospective studies that integrate molecular and pharmacogenetic studies are crucial for revealing the mechanism and preventive medical efforts.Öğe The Effect of Morphine Dependence and Morphine Withdrawal on Neuritin and Some Markers of Neurogenesis in the Rat Hippocampus(Wiley, 2023) Cimen, Yasin Ali; Yildiz, Ibrahim; Gunes, Canan Eroglu; Ozkurkculer, Alpaslan; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe Effect of Morphine Dependency on Apelinergic System in Rat Hippocampus(Wiley, 2022) Yildiz, Ibrahim; Cimen, Yasin Ali; Eroglu, Canan; Ozkurkculer, Alpaslan; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe Effect of Naloxone Precipitated Morphine Withdrawal on Melatonin Receptor Expressions in Hippocampus and Hypothalamus Tissues in Morphine Dependent Rat(Karger, 2021) Cimen, Yasin Ali; Gunes, Canan Eroglu; Yildiz, Ibrahim; Ozdengul, Faik; Kurar, Ercan; Meral, Ismail; Kutlu, Selim[Abstract Not Availabe]Öğe Effect of Pregnancy and Lactation Period on Depression Like Behaviour in Depressive Rats(Karger, 2018) Solak, Hatice; Koca, Raviye Ozen; Koc, Aynur; Sahin, Zafer; Uguz, Faruk; Ak, Mehmet; Kurar, Ercan[Abstract Not Availabe]Öğe Effect of RFRP-3 on Anxiety-like Behaviour and Hippocampal Neurogenesis in Rats(Wiley-Blackwell, 2016) Boyukcam, Yasemin; Sahin, Zafer; Koc, Aynur; Eroglu, Canan; Gormus, Isik Solak; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe The Effect of Sertraline on Cognitive Behaviors in Depressed Rats with Chronic Mild Stress Model(Wiley, 2022) Solak, Hatice; Gormus, Z. Isik Solak; Koca, Raviye Ozen; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe Effects of Mu Opioidergic Receptors on Endoplasmic Reticulum Stress in the Hypothalamus on Rats(Wiley, 2023) Sen, Aysu; Gunes, Canan Eroglu; Caliskan, Zeynep; Erkaya, Zeliha; Dursun, Ebru Nur; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe ELA-DRA gene haplotypes in healthy Arabian horses(Elsevier Science Bv, 2016) Bulut, Zafer; Kurar, Ercan; Bulut, Oya; Nizamlioglu, Mehmet[Abstract Not Availabe]Öğe Fibrinogen-A? THR312ALA Polymorphism is Associated to Chronic Thromboembolic Pulmonary Hypertension in Turkey(Kare Publ, 2023) Cortuk, Mustafa; Yildizeli, Bedrettin; Kurar, Ercan; Kayis, Seyit Ali; Tanriverdi, Elif; Cetinkaya, ErdoganBackground: Chronic thromboembolic pulmonary hypertension is a condition that occurs after mechanical obstruction of the pulmonary arteries by thrombus. Since the frequency and demographics of chronic thromboembolic pulmonary hypertension differ between countries, it is thought that genetic factors may play a role in its development. The aim of this study is to reveal the status of VKORC1, CYP2C9*3, CYP2C9*7, and fibrinogen-A alpha THR312ALA gene polymorphisms in chronic thromboembolic pulmonary hypertension patients in Turkey.Methods: In this prospective cross-sectional study, a total of 46 chronic thromboembolic pulmonary hypertension patients and 106 healthy volunteers were included. Polymerase chain reaction-restriction fragment length polymorphism method was used to determine candidate gene polymorphisms for chronic thromboembolic pulmonary hypertension. The general population parameters of each locus were calculated, and the relationship between dominant, co-dominant, and recessive genotype models and chronic thrombo-embolic pulmonary hypertension was analyzed.Results: For the fibrinogen-A alpha gene, those with the THR/THR genotype were found to have a 13.51 (95% CI: 2.688-33.333) times less susceptibility rate to the disease than those with the ALA/ THR genotype, the susceptibility of THR/ALA genotype to the disease was 5.026 (95% CI: 1.774-14.242) times more than those with ALA/ALA genotype. There was no difference between patient groups for VKORC1, CYP2C9*3 genes (P > .05). Since the CYP2C9*7 patient group was monomorphic for the ILE allele, the patient/control odds ratio and 95% CI could not be calculated.Conclusion: This study shows that there is an association between the fibrinogen-A alpha gene ALA polymorphism at the amino acid position of 312 and the development of chronic thromboembolic pulmonary hypertension, but not between the CYP2C9 and VKORC1 gene polymorphisms.Öğe Fibrinogen-A? THR312ALA Polymorphism is Associated to Chronic Thromboembolic Pulmonary Hypertension in Turkey(Kare Publ, 2023) Cortuk, Mustafa; Yildizeli, Bedrettin; Kurar, Ercan; Kayis, Seyit Ali; Tanriverdi, Elif; Cetinkaya, ErdoganBackground: Chronic thromboembolic pulmonary hypertension is a condition that occurs after mechanical obstruction of the pulmonary arteries by thrombus. Since the frequency and demographics of chronic thromboembolic pulmonary hypertension differ between countries, it is thought that genetic factors may play a role in its development. The aim of this study is to reveal the status of VKORC1, CYP2C9*3, CYP2C9*7, and fibrinogen-A alpha THR312ALA gene polymorphisms in chronic thromboembolic pulmonary hypertension patients in Turkey.Methods: In this prospective cross-sectional study, a total of 46 chronic thromboembolic pulmonary hypertension patients and 106 healthy volunteers were included. Polymerase chain reaction-restriction fragment length polymorphism method was used to determine candidate gene polymorphisms for chronic thromboembolic pulmonary hypertension. The general population parameters of each locus were calculated, and the relationship between dominant, co-dominant, and recessive genotype models and chronic thrombo-embolic pulmonary hypertension was analyzed.Results: For the fibrinogen-A alpha gene, those with the THR/THR genotype were found to have a 13.51 (95% CI: 2.688-33.333) times less susceptibility rate to the disease than those with the ALA/ THR genotype, the susceptibility of THR/ALA genotype to the disease was 5.026 (95% CI: 1.774-14.242) times more than those with ALA/ALA genotype. There was no difference between patient groups for VKORC1, CYP2C9*3 genes (P > .05). Since the CYP2C9*7 patient group was monomorphic for the ILE allele, the patient/control odds ratio and 95% CI could not be calculated.Conclusion: This study shows that there is an association between the fibrinogen-A alpha gene ALA polymorphism at the amino acid position of 312 and the development of chronic thromboembolic pulmonary hypertension, but not between the CYP2C9 and VKORC1 gene polymorphisms.Öğe Genetic Diversity of Eight Domestic Goat Populations Raised in Turkey(Hindawi Ltd, 2016) Bulut, Zafer; Kurar, Ercan; Ozsensoy, Yusuf; Altunok, Vahdettin; Nizamlioglu, MehmetThe objective of this study was to determine the intra- and intergenetic diversities of eight different goat populations in Turkey including Hair, Angora, Kilis, Yayladag, Shami, Honamli, Saanen, and Alpine. A total of 244 DNA samples were genotyped using 11 microsatellites loci. The genetic differentiation between breeds was considerable as a result of the statistically significant (P < 0.001) pairwise F-ST values of each pair of breeds. Exceptionally, F-ST values calculated for Honamli and Hair breeds were statistically nonsignificant (P > 0.05). Heterozygosity values ranged between 0.62 and 0.73. According to the structure and assignment test, Angora and Yayladag goats were assigned to the breed they belong to, while other breeds were assigned to two or more different groups. Because this study for the first time presented genetic data on the Yayladag goat, results of structure analysis and assigned test suggest that further analyses are needed using additional and different molecular markers.Öğe Glycoside oleandrin downregulates toll-like receptor pathway genes and associated miRNAs in human melanoma cells(Elsevier, 2022) Gunes, Canan Eroglu; Celik, Fatma Secer; Secme, Mucahit; Elmas, Levent; Dodurga, Yavuz; Kurar, ErcanMelanoma accounts for the majority of skin cancer-related deaths. Nerium oleander is a plant known to be toxic and consumed due to the cardiac glycosides it contains. Oleandrin is a cardiac glycoside obtained from of N. oleander. Beside capable of inhibiting proliferation and metastasis of cancer cells, cardiac glycoside derivative compounds cause cardiovascular side effects. Because of cardiovascular toxicity of clinically used cardiac gly-cosides, it is necessary to investigate cardiac glycoside derivative compounds capable of inhibiting proliferation and metastasis of cancer cells.It is known that oleandrin has anticarcinogenic effects in other cancers. Previous studies have shown that toll -like receptors (TLRs) and their related microRNAs (miRNAs) are associated with cancer. Therefore, aim was to investigate the effect of oleandrin on genes and miRNAs associated with TLRs in A375 melanoma cells in this study. The effects of oleandrin on cell viability, cytokines, apoptosis were evaluated using XTT, ELISA and TUNEL analyses, respectively. The effect of oleandrin on expression of TLR genes and 5 associated miRNAs in A375 cells has been determined by qRT-PCR. In addition, the levels of MyD88, TLR2 and TLR4 proteins were analyzed by western blot method.ELISA indicated that oleandrin treatment (47 nM at 48 h) reduced the level of proinflammatory cytokine IFNG. TUNEL analysis showed that apoptosis rate was significantly increased in the oleandrin dose group. According to qRT-PCR results, there was a significant decrease in IRAK1, IRAK4, MyD88, TLR2-TLR7 and TRAF3 expressions in the oleandrin treated group compared to the control (untreated cell). Also, a significant decrease in TLR4 protein expression has been observed. In addition, oleandrin significantly downregulated the levels of hsa-miRNA-146a-5p and hsa-miRNA-21-5p.In conclusion, it has been observed that oleandrin has an effect on TLR pathway-related genes and miRNAs in melanoma cells. We show that TLRs pathways and hsa-miR-146a-5p and hsa-miR-21-5p can participate in the oleandrin molecular mechanism of action.Öğe Hippocampal and Hypothalamic FBN1 Expression in an Experimental Opioid Addiction Rat Model(Karger, 2021) Gunes, Canan Eroglu; Cimen, Yasin Ali; Yildiz, Ibrahim; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe Hypothalamic Expressions of Apelin, Apelin Receptor and Neuritine in the First Generation Rat Pups: A Maternal Depression Model(Karger, 2021) Kurar, Ercan; Gunes, Canan Eroglu; Koca, Raviye Ozen; Solak, Hatice; Koc, Aynur; Sahin, Zafer; Cuce, Gokhan[Abstract Not Availabe]Öğe Intrauterine detection of DCLRE1C (Artemis) mutation by restriction fragment length polymorphism(Wiley, 2019) Karaselek, Mehmet Ali; Kapakli, Hasan; Keles, Sevgi; Guner, Sukru Nail; Celik, Seyma Celikbilek; Kurar, Ercan; Reisli, Ismail[Abstract Not Availabe]
