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    Comparison of the GenoType® MTBC Molecular Genetic Assay with culture methods in the diagnosis of tuberculosis
    (Termedia Publishing House Ltd, 2014) Kurtoglu, Muhammet Guzel; Ozdemir, Mehmet; Kesli, Recep; Baysal, Bulent
    Introduction: Clinical samples from 433 patients pre-diagnosed with tuberculosis in Konya, Turkey, were investigated prospectively to compare the GenoType (R) MTBC test (GenoType (R) MTBC) with conventional gold standard culture methods. Material and methods: Lowenstein Jensen (LJ) and Mycobacteria Growth Indicator Tube (MGIT)-960 culture methods and GenoType (R) MTBC were performed together. Results: Mycobacterium tuberculosis (M. tuberculosis) detection rates were 16.2% by culture methods, 15.4% by GenoType (R) MTBC, and 6% by acid-fast bacilli microscopy. The LJ or MGIT-960 with GenoType (R) MTBC detected M. tuberculosis in 12 samples each that were negative according to the other culture method alone. Among 70 M. tuberculosis-positive samples, detection rates were 37% (26/70) by microscopy and 82.8% (58/70) by LJ and MGIT-960, but 95.7% (67/70) by GenoType (R) MTBC. Conclusions: GenoType (R) MTBC may be used as a beneficial adjunct test to culture methods for the detection of M. tuberculosis.
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    Detection of the frequency of PER-1 type extended-spectrum ?-lactamase-producing Acinetobacter baumannii clinical isolates in Turkey: a multicenter study
    (Tubitak Scientific & Technological Research Council Turkey, 2014) Asik, Gulsah; Ozdemir, Mehmet; Kurtoglu, Muhammet Guzel; Yagci, Server; Oksuz, Lutfiye; Gul, Mustafa; Kocoglu, Mucahide Esra
    Background/aim: beta-Lactamases are an important resistance mechanism in Acinetobacter baumannii. Pseudomonas extended-resistance (PER-1) type beta-lactamase-producing strains have been reported from various geographic locations; however, PER-1 type beta-lactamases from Turkish hospitals have not been investigated extensively. The aim of this study was to determine the prevalence of PER-1 type beta-lactamases in A. baumannii isolates in various regions of Turkey. Materials and methods: A total of 763 clinical A. baumannii isolates were collected from 9 university hospitals and 2 state hospitals between 2008 and 2011. Molecular amplification of the OXA-51 gene from the A. baumannii genome was performed in order to verify identification of the species. Real-time polymerase chain reaction was used to detect bla(PER-1) genes. Results: PER-1 was detected in 24.6% of the isolates. The annual frequencies of the PER-1 enzyme were detected as 52.2%, 35.9%, and 8.3% in 2008, 2009, and 2010, respectively. PER-1 prevalence decreased gradually over time. The differences observed in PER-1 prevalence among the regions of Turkey were statistically significant (chi-square test; P < 0.001). Conclusion: These data demonstrate that the frequency of detection of PER-1 type beta-lactamases in A. baumannii species has decreased in Turkey. However, the increased carbapenem resistance, together with multidrug resistance, has created a worrisome situation regarding this pathogen.
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    Prevalence and Concomitancy of Respiratory Viruses in Children with Acute Respiratory Tract Infections
    (Georg Thieme Verlag Kg, 2016) Tuzuner, Ugur; Akkaya, Oya; Ozdemir, Mehmet; Kurtoglu, Muhammet Guzel
    Respiratory tract infections (RTI) are among the most commonly seen infections in children across the world, with the highest rate of morbidity. Viruses are known to be the primary agent in these infections and mostly lead to upper RTIs. The most commonly seen viral agents of RTIs may be listed as rhinoviruses (HRV), influenza, parainfluenza (PIV), respiratory syncytial virus (RSV), adenovirus, and enteroviruses. In this study, prediagnosed children with acute RTI between January 2013 and May 2015 were included. Nasopharyngeal swab samples obtained from a total of 2,268 patients admitted to the hospital of Meram Medical School of Necmettin Erbakan University and Ministry of Health Konya Training and Research Hospital were investigated. The nasopharyngeal swab samples obtained in the hospital of Meram Medical School were investigated via Seeplex RV12 ACE Detection multiplex PCR (Seegene, South Korea) while the samples in Konya Training and Research Hospital were assessed using the system by CLART PneumoVir (Genomica, Spain). Of the 2,268 samples investigated in the study, 1,320 (58.2%) were detected for viruses. Positive number of viruses found in both kits were collected for use in a table, and the most common viruses rates were calculated according to these numbers. Among positive 1,221 samples, 27.9% were found to be RSV B, and this rate was, in turn, followed by HRV (18.8%) and RSV A. The most frequent concomitant of double viral agents was observed in RSV B and HRV as 14% followed by RSV A and HRV as 6.8%, and PIV 3 and HRV with 5.9%. While RSV B and RSV A were encountered most in winter and spring months, HRV was determined as an infectious agent in all seasons. Multiplex polymerase chain reaction is beneficial for physicians to diagnose such viruses at an early stage. By the early detection of respiratory viruses leading to seasonal epidemics, physicians' approach to patients will become easier, and unnecessary use of antibiotics will be prevented. Additionally, our study findings are intriguing in shedding light on studies related to the development and application of vaccines.