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Öğe The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamol(Wiley, 2015) Hacibeyoglu, Gulcin; Saritas, Tuba Berra; Saritas, Zulfikar Kadir; Korkmaz, Musa; Sevimli, Alper; Mehmetoglu, Idris; Otelcioglu, SerefThis study was conducted to investigate possible histopathological effects and biochemical reflections of intra-articular dexketoprofen trometamol. A total of 24 New Zealand rabbits were included in the study. Blood sampling was carried out from all animals on the first day, then they were randomly allocated either to the control group (GroupC, n=9) or the dexketoprofen trometamol group (GroupD, n=15). GroupC underwent each two intra-articular injections of saline, 0.25mL into right and 0.50mL into left knee. Group D was injected 0.25mL (6.25mg) dexketoprofen trometamol into the right knee and 0.50mL (12.5mg) into the left. The groups were divided randomly into three. Tissue and blood samples were collected from Groups C1 and D1 on the first day, C2 and D2 on the second day and C3 and D3 on the 10th day of the study. Interleukin-1 (IL-1), interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-) and C-reactive protein (CRP) levels were studied. The histopathological examination of C and D groups did not present any deterioration. IL-6 basal levels were significantly higher in Group D2 compared with C2 and C3 compared with D3. Basal TNF- levels were higher compared with day 1 in Group C1, and IL-6 and CRP levels were higher in Group D3. Also, none of the increases in these values are supported by histopathological evaluation results. Consequently, we suppose that dexketoprofen trometamol does not cause histopathological deterioration in articular cartilage of rabbits, and the increases in biochemical parameters exclusively are not clinically significant.Öğe EFFECTS OF PHOTOTHERAPY ON SERUM PARAOXONASE ACTIVITY AND TOTAL ANTIOXIDANT CAPACITY IN NEWBORN JAUNDICE(Nobel Ilac, 2014) Kurban, Sevil; Annagur, Ali; Altunhan, Huseyin; Mehmetoglu, Idris; Ors, Rahmi; Erdem, Said Sami; Yerlikaya, Fatma HumeyraObjective: Phototherapy is an efficient and commonly used form of therapy for the treatment of neonatal hyperbilirubinemia. Paraoxonase 1 (PON1) is an anti oxidative enzyme, which eliminates lipid peroxides. The aim of our study was to investigate the effect of phototherapy on serum PON1 activity and total antioxidant capacity (TAC) in hyperbilirubinemic full-term newborns. Material and Method: The study was performed on 40 full-tenn newborns between 3 to 15 days of age exposed to phototherapy. SerumPON1 activity and TAC levels of the babies were determined before and after phototherapy by spectrophotometric assays. Results: We have found that PON1 activity was not significantly affected by phototherapy whereas TAC levels were decreased significantly after phototherapy (p<0.001). Conclusion: Our findings demonstrated that phototherapy has no direct effect on PON1 activity. Also, decreased TAC levels might have resulted from increased oxidative stress which may lead to consumption of antioxidant molecules.Öğe Increased ischaemia-modified albumin is associated with inflammation in acute rheumatic fever(Cambridge Univ Press, 2014) Karatas, Zehra; Baysal, Tamer; Sap, Fatih; Alp, Hayrullah; Mehmetoglu, IdrisIntroduction: Ischaemia-modified albumin, a novel biochemical marker for tissue ischaemia, was found to be associated with oxidative stress. The purpose of this study was to assess the role of ischaemia-modified albumin in the diagnosis of acute rheumatic fever and also to evaluate the ischaemia-modified albumin levels in children with heart valve disease. Methods: The study groups, aged 5-18 years, consisted of 128 individuals - 40 with acute rheumatic fever, 35 with congenital heart valve disease, 33 with chronic rheumatic heart disease, and 20 healthy control subjects. Results: The ischaemia-modified albumin, erythrocyte sedimentation rate, and C-reactive protein levels of the acute rheumatic fever group were significantly higher than those in the chronic rheumatic heart disease, congenital heart valve disease, and control groups, separately (p < 0.001). The ischaemia-modified albumin levels in both carditis and isolated arthritis subgroups of children with acute rheumatic fever were significantly higher than in the control group (p < 0.001, p < 0.01, respectively). However, there was no statistically significant difference between the chorea subgroup and control subjects. In addition, significant correlations were observed between ischaemia-modified albumin and acute phase reactants of patients with acute rheumatic fever (p < 0.001 for both erythrocyte sedimentation rate and C-reactive protein). The ischaemia-modified albumin levels of chronic rheumatic heart disease, congenital heart valve disease, and control subjects were similar. Conclusions: The increased level of ischaemia-modified albumin in children with acute rheumatic fever seems to be associated with inflammation. However, further studies are needed to provide stronger evidence.Öğe Investigation of Oxidative Stress Markers in Essential Hypertension(Clin Lab Publ, 2013) Toker, Aysun; Mehmetoglu, Idris; Yerlikaya, F. Humeyra; Nergiz, Suleyman; Kurban, Sevil; Gok, HasanBackground: The main goal of this study was to evaluate ischemia modified albumin (IMA), total antioxidant status (TAS), and total oxidant status (TOS) levels in treated essential hypertensive patients and to compare them with levels of normotensive subjects. Methods: In 45 hypertensive and 30 control subjects, serum levels of IMA were determined manually using a spectrophotometric Co(II)-albumin binding assay. TAS and TOS levels were evaluated spectrophotometrically. Lipid profile was estimated by routine methods. Results: Hypertensive patients had significantly higher levels of TOS and IMA (p = 0.020 and p = 0.034, respectively) and lower levels of TAS (p = 0.016) in comparison with control subjects. Serum levels of TAS were negatively correlated with TOS and IMA levels in the patient group. Serum levels of TOS were also positively correlated with IMA levels. There was no significant correlation between blood pressure and TAS, TOS, and IMA levels. Conclusions: Our results showed higher levels of IMA in hypertensive patients. We suggest that higher levels of IMA may result from increased oxidative stress and decreased antioxidant status in hypertensive patients.Öğe INVESTIGATION OF THE RELATIONSHIPS OF OBESITY WITH MELATONIN AND DEHYDROEPIANDROSTERONE LEVELS(Nobel Ilac, 2017) Mehmetoglu, Idris; Gokce, Seyid; Kurban, Sevil; Gokce, Recep; Atalar, Mehmet Nuri; Celik, MuhammedObjective: Melatonin is synthesized in pineal gland and plays a role in energy metabolism and the weight-reducing effects. Dehydroepiandrosterone (DHEA) is synthesized primarily by the zona reticularis of adrenal cortex and to a lesser degree by the gonads. DHEA has antioxidant, antilipidperoxidative, antiinflammatory, antiatherogenic, antiaging and weight-reducing or preventing effects. Our aim of this study was to investiagate blood DHEA, melatonin, insulin resistance (IR) and lipids levels and correlations between them in obese and nonobese subjects. Material and Method: The study was performed on 33 obese subjects ((16 male (M), 17 female (F)) aged 40.03 +/- 8.47 years and 33 normal weight healty controls (15M, 18F) aged 28.76 +/- 6.22 years. In both groups, DHEA, melatonin, IR, high-density lipoprotein (HDL), low-density lipoprotein (LDL) and total cholesterol and triglyceride levels and correlations between them were investigated. DHEA and melatonin levels were determined by ELISA method and insulin, fasting blood glucose levels and lipid parameters were determined by rutin method using commercially avilable kits. IR was calculated using a formula. Results: There was no statistically significant differences between DHEA and melatonin levels of the groups and there were no correlations between them in both groups. However, there was a significant negative correlation between DHEA and triglyceride levels in the obese group (r=-0.342, p<0.05). Conclusion: Our results show that DHEA and melatonin levels were not changed in obesity and they have no effects on IR. However significant negative correlation between DHEA and triglyceride levels in obese subjects needs to be more investigated in terms of the effect of DHEA on adipose tissue and obesity.Öğe Lipoprotein-associated phospholipase-A2 activity and its diagnostic potential in patients with acute coronary syndrome and acute ischemic stroke(Wolters Kluwer Medknow Publications, 2017) Kocak, Sedat; Ertekin, Birsen; Girisgin, Abdullah Sadik; Dundar, Zerrin Defne; Ergin, Mehmet; Mehmetoglu, Idris; Bodur, SaidBackground: The study examined the Lp-PLA(2) activity at the patients presented to the emergency department with acute coronary syndrome (ACS) or acute ischemic stroke (AIS), as well as its diagnostic value. Methods: The prospective study included consecutive male and female patients aged >18 years that presented to the our emergency department with ACS or AIS between November 2009 and January 2010. Blood samples were obtained immediately following diagnosis in the ACS and AIS groups. The diagnostic value of Lp-PLA(2) was determined based on receiver operating characteristic curves, sensitivity, specificity, predictive values, likelihood ratios and accuracy rates. Results: In all, 34 ACS and 32 AIS patients were included in the study, and the control group included 35 patients. Lp-PLA(2) enzyme activity was significantly lower in the ACS and AIS groups than in the control group (26.7 +/- 13.8, 31.4 +/- 13.6, and 41.4 +/- 8.1 nmol min(-1).mL(-1), respectively; p < 0.0001, p = 0.022). In the ACS group the area under the curve (AUC) was 0.825 (95% CI: 0.722-0.929), sensitivity was 71% for an optimal Lp-PLA(2) cut-off value of 31.4 nmol min(-1).mL(-1), and specificity was 91%, whereas in the AIS group the AUC was 0.768 (95% CI: 0.652-0.884), sensitivity was 75% for an optimal Lp-PLA(2) cut-off value of 38.1 nmol min(-1).mL(-1), and specificity was 74%. Conclusions: Lp-PLA(2) enzyme activity was significantly lower during the early stage of both ACS and AIS. The obtained statistic data suggest that low Lp-PLA(2) enzyme activity can be used for diagnostic purposes. Copyright (C) 2016 The Emergency Medicine Association of Turkey. Production and hosting by Elsevier B.V. on behalf of the Owner. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Öğe Obesity Is an Independent Determinant of Ischemia-Modified Albumin(Karger, 2012) Mehmetoglu, Idris; Kurban, Sevil; Yerlikaya, Fatma Humeyra; Polat, HakkiObjective: We have measured ischemia-modified albumin (IMA), total antioxidant status (TAS), total oxidant status (TOS) and high-sensitivity C-reactive protein (hsCRP) levels in obese and normal-weight subjects to investigate if IMA can be used as a biomarker of oxidative stress and inflammation and if IMA was an independent determinant of obesity or not. Methods: The study was performed on 92 obese subjects (20 male, 72 female) aged 38 +/- 11 years and 78 normal-weight controls (19 male, 59 female) aged 37 +/- 11 years. Serum lipids, IMA, TAS, TOS, and hsCRP levels of the subjects were measured. Results: IMA (p < 0.05), TOS (p < 0.001), and hsCRP (p < 0.001) levels of the obese subjects were significantly higher, whereas TAS levels were significantly lower (p < 0.05) than those of the controls after adjustment for age and gender. In the linear regression analysis, waist circumference (r(2) = 0.139, p < 0.01), BMI (r(2) = 0.136, p < 0.01) and insulin (r(2) = 0.120, p < 0.05) were shown to be significant independent determinants of IMA levels. Conclusions: We have found that oxidative stress and inflammation were increased and antioxidative defense was decreased, which resulted in increased levels of IMA, a biomarker of ischemia, in obese subjects. Also, obesity and insulin were found to be independent determinants of IMA. Thus, obese subjects are under high risk of ischemia, and IMA may be used as a biomarker of oxidative stress and ischemia. Further larger investigations are needed to confirm this opinion. Copyright (C) 2012 S. Karger GmbH, FreiburgÖğe Plasma 9-cis, 11-trans ve 10-trans, 12-cis Conjugated Linoleic Acid Isomers Levels in Continuous Ambulatory Peritoneal Dialysis Patients(Turk Nefroloji Diyaliz Transplantasyon Dergisi, 2012) Yerlikaya, Fatma Hmeyra; Mehmetoglu, Idris; Kurban, Sevil; Tonbul, Halil ZekiOBJECTIVE: Peritoneal dialysis is a renal replacement therapy in patients with end stage renal disease. Conjugated linoleic acid (CLA) refers to a class of positional and geometric conjugated isomers of the omega-6 essential fatty acid, linoleic acid. This study aimed to investigate biologically active isomers of CLA (10-trans, 12-cis CLA and -9-cis, 11-trans CLA) and the relationship between these fatty acids and weight, body mass index (BMI), C-reactive protein (CRP) and parathyroid hormone (PTH) in continuous ambulatory peritoneal dialysis patients (CAPD) and healthy subjects. MATERIAL and METHODS: This study was performed on 51 (21M, 30F) CAPD patients at least 6 months under dialysis, aged 20-75 years and 45 (25M, 20F) healthy control subjects aged 20-60 years. Plasma 10-trans, 12-cis CLA (t10, c12 CLA) and 9-cis, 11-trans CLA (c9, t11 CLA) levels were measured by GS-MS technique. RESULTS: t10, c12 and c9, t11 isomers of CLA levels of the CAPD patients were as follows: 11.84 +/- 5.3 and 8.34 +/- 4.4 mg/L respectively. The same parameters of the controls were as follows: 12.88 +/- 4.1 and 9.20 +/- 4.5 mg/L, respectively. The differences between plasma CLA isomers levels of CAPD patients and control subjects were not significant. Isomers of CLA negatively correlated with serum PTH levels in CAPD patients. However, there was no significant correlation between plasma isomers of CLA and weight, BMI, CRP in CAPD patients. CONCLUSION: These findings suggest that a CLA-rich diet may help in reducing high levels of parathyroid hormone in the case of good control of phosphorus in CAPD patients associated with secondary hyperparathyroidism.Öğe Serum ischemia-modified albumin and oxidized LDL in cord blood and serum of neonates born to pre-eclamptic mothers(Wiley, 2015) Mehmetoglu, Idris; Kurban, Sevil; Toker, Aysun; Annaguer, Ali; Altunhan, Huseyin; Erbay, Ekrem; Ors, RahmiBackgroundWe investigated oxidized low-density lipoprotein (OxLDL) and ischemia-modified albumin (IMA) in cord blood and neonatal blood of 7-day-old neonates born to pre-eclamptic and normotensive healthy mothers. MethodsThe study was performed on 30 neonates born to pre-eclamptic and 20 neonates born to normotensive mothers. IMA and OxLDL were determined on spectrophotometry and ELISA, respectively. ResultsIMA in cord blood was higher in the pre-eclamptic group as compared with the normotensive group, but the difference between the groups was not significant. IMA in neonate venous blood was significantly higher in the pre-eclamptic group than in the normotensive group (P < 0.001). OxLDL in both cord blood and in neonate venous blood was significantly higher in the pre-eclamptic group compared with the normotensive group (P < 0.001). IMA and OxLDL were significantly decreased after delivery in both groups. ConclusionsSignificantly increased cord blood OxLDL and significantly increased OxLDL and IMA 7days after birth in neonates born to pre-eclamptic mothers might be an indicator of increased oxidative stress in pre-eclampsia.Öğe Serum ischemia-modified albumin levels at diagnosis and during treatment of late-onset neonatal sepsis(Taylor & Francis Ltd, 2014) Yerlikaya, F. Humeyra; Kurban, Sevil; Mehmetoglu, Idris; Annagur, Ali; Altunhan, Huseyin; Erbay, Ekrem; Ors, RahmiSepsis is one of the most common infectious conditions in the neonatal period, and continues as a major source of morbidity and mortality. The aim of this study is to determine serum ischemia-modified albumin (IMA) levels in late-onset neonatal sepsis at the time of diagnosis and after therapy, and to show the meaningful on the follow-up. Also, it is aimed to compare serum IMA levels with serum C-reactive protein (CRP), procalcitonin (PCT) levels and white blood cell count. The study was performed on 33 premature babies with sepsis and 21 healthy premature controls at 7-28 days of age. In the sepsis group, biochemical parameters and blood culture samples were obtained from the blood at the onset and on the fifth day of treatment for each patient. Serum IMA, CRP, PCT and white blood cell count were significantly higher in the sepsis group before treatment when compared with the control group. In addition, the levels of IMA were positively correlated with white blood cell count, CRP and PCT in the sepsis group before treatment. In conclusion, serum IMA levels may be useful in late-onset neonatal sepsis at the time of diagnosis and after therapy. As far as we know this is the first report about the assesment of illness diagnosis and after therapy using serum IMA levels, and further studies are needed to confirm our results in larger groups of patients.