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    Interfacial characteristics of BIOfactor MTA and Biodentine with dentin
    (Wiley, 2023) Akbulut, Makbule Bilge; Mutlu, Seref Nur; Soylu, Mehmet Ali; Simsek, Emine
    The objective of this study was to characterize the interface between dentin and MTA-Angelus (Angelus, Londrina, Brasil), Biodentine (Septodont, France) and BIOfactor MTA (Imicryl, Konya, Turkey) using scanning electron microscopy (SEM), energy disperse X-ray spectroscopy (SEM-EDS) and confocal laser scanning microscopy (CLSM). Fifteen dentin segments were obtained from previously extracted single-rooted human teeth. Canal lumens were instrumented with diamond burs and then randomly filled with MTA-Angelus, Biodentine or BIOfactor MTA and placed in distilled water or Hanks' Balanced Salt Solution (HBSS) for 28-days. The samples were examined with SEM and the thickness of the interfacial layer measured. SEM-EDS analysis was performed to determine principal elemental composition of the material, dentin, and interfacial area. The marginal adaptation of cements to dentin was assessed by confocal microscopy and the percentage of material penetration was calculated. An interfacial layer was evident in approximately 70% of SEM images in both MTA-Angelus and BIOfactor samples. The thickness of interfacial layer was significantly higher in HBSS than in distilled water for all groups. MTA Angelus resulted in the thickest interfacial layer in distilled water while Biodentine had the thickest interfacial layer in HBSS. Calcium levels within the BIOfactor MTA-dentin interface were higher than both dentin and cement. Dentin penetration was higher in BIOfactor MTA and silicon was evident in all material-dentin interfaces. All calcium silicate-based materials promoted the formation of an interfacial layer. BIOfactor MTA exhibited promising characteristics with its good marginal adaptation even though it presented a moderately thick interfacial layer. Research Highlights A distinguishable interfacial layer was observed in most of the samples within the BIOfactor MTA, MTA-Angelus and Biodentine groups. The elemental constitution of the interfacial layer was different from that of the calcium silicate based materials.
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    Tooth Discoloration Effect of BIOfactor Mineral Trioxide Aggregate: A 6-Month In Vitro Study
    (Mdpi, 2023) Mutlu, Seref Nur; Akbulut, Makbule Bilge
    The central objective of this research was to examine the color consistency of three materials, Biodentine (Septodont, France), Angelus MTA (Angelus, Brasil), and BIOfactor MTA (Imicryl, Turkey), when exposed to various irrigation solutions and to observe their impact on tooth discoloration. Each material was used to make sample cylinders (n = 18). After hardening, the samples were immersed for 24 h in either distilled water, 2.5% sodium hypochlorite (NaOCl), or 2% chlorhexidine gluconate (CHX). A spectrophotometer was used to measure the color changes. On the mandibular molars, access cavities were made in order to assess the discoloring impact of calcium silicate cements. In the pulp chambers of the teeth, Biodentine, Angelus MTA, and BIOfactor MTA were inserted (n = 17). Glass ionomer cement was used to seal the samples. Spectrophotometric measurements were made at five different intervals (the beginning, one week, one month, three months, and six months), after which color variance values were computed. The resulting information was statistically evaluated. In all solutions, Biodentine and BIOfactor MTA displayed similar & UDelta;E values. When Angelus MTA was soaked in NaOCl, it discolored more than in CHX or distilled water. Distilled water and NaOCl both caused identical discoloration on all material groups when solutions were examined separately. With CHX, Biodentine changed colors more significantly. After a week and a month, all substances caused comparable tooth discoloration. Biodentine produced the most significant color shifts on teeth at the third and sixth months. Angelus MTA exhibited less color stability in NaOCl and Biodentine in CHX. While Biodentine induced significant discoloration, BIOfactor MTA only showed a moderate amount.