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Öğe Can Monkeypox Infection Be Serious Problem for Children?(Georg Thieme Verlag Kg, 2022) Ugur, Ayse Ruveyda; Ozdemir, Mehmet[Abstract Not Availabe]Öğe A case of Human Parechovirus Infection in an Infant with Meningitis(Aves Yayincilik, Ibrahim Kara, 2021) Tokak, Semih; Ozdemir, Mehmet; Gulseren, Yasemin Derya; Caksen, HuseyinHuman parechovirus is a potentially serious viral infection in neonates and infant and its importance increasing by years. In young infants, the typical clinical presentation includes fever, severe irritability, and rash, often leading to descriptions of hot, red, angry babies. We report a case of a 43-day-old girl with a fever that required hospitalization and in which human parechovirus was identified in the cerebrospinal fluid. Blood, urine, and cerebrospinal fluid bacterial cultures of the patient were negative and the patient has improved.Öğe Changing Infection Dynamics with the Pandemic: Distribution of Viral Agents of Respiratory Tract Infections in the Last 5 Years(Galenos Publ House, 2023) Karabey, Mehmet; Kaya, Havva; Kaba, Kadir; Ceylan, Alperen; Taskin, Zekeriya; Ozdemir, Mehmet; Feyzioglu, BahadirIntroduction: The measures taken against Severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2) positively impacted the reduction of its transmission. In addition, these measures also significantly decreased the spread of infections caused by other respiratory viruses. In this study, we aimed to determine the frequency of respiratory virus infections, other than SARS-CoV-2, during the Coronavirus disease-2019 (COVID-19) pandemic and investigated their course during both the quarantine and normalization periods.Materials and Methods: Swab samples sent to Necmettin Erbakan University Meram Faculty of Medicine Hospital Medical Microbiology Laboratory between May 2017 and May 2022 to determine the viral agents of respiratory tract infections by polymerase chain reaction (PCR) were retrospectively scanned.Results: A total of 187,240 SARS-CoV-2 PCR tests were performed between April 1, 2020, and May 31, 2020, and 14,773 (9.82%) tests were reported as positive. Based on our observation, the viruses demonstrating a decrease during the pandemic period were influenza A and B, seasonal H1N1, human metapneumovirus, respiratory syncytial virus A and B, and human herpes virus 7. No changes were observed in the infection rates of parvovirus B19, adenovirus, and human rhinovirus.Conclusion: In our study, we observed a serious decline in the cases caused by other respiratory viral agents and the detection rates of these agents during the pandemic period compared to the pre-pandemic period. This can be attributed to the extensive impact of the measures implemented during the COVID-19 pandemic to mitigate the spread of respiratory infections. Our results are a reflection of this situation. We believe that the data obtained from a large number of samples will serve as a guide for managing infections during the current pandemic and for future experiences.Öğe Comparative Evaluation of In-House and Commercial Real-Time PCR Methods for the Detection of the BK and JC Viruses in Clinical Samples(Thieme Medical Publ Inc, 2020) Ozdemir, Mehmet; Ayan, Ugur; Sevik, MuratAim The two most common human polyomaviruses are the BK (BKV) and JC viruses (JCV). Diseases associated with polyomavirus usually occur in cases of severe cellular immunosuppression. BKV and JCV can cause many diseases, especially if they are reactivated in an immunosuppressed host. The aim of this study is to compare and evaluate the results of real-time polymerase chain reaction (PCR) methods targeting the small and large T gene regions of the viral genome, considering polymorphisms occurring in the viral genome of BKV and JCV. Materials and Methods Urinary specimens of 82 patients were taken from immunosuppressed patient and sent to molecular microbiology laboratory of Meram Medical Faculty. The small t gene was investigated using a commercial kit (LightMix, Roche) by real-time PCR method. Large T gene was investigated by using the optimized in-house real-time PCR method. Sequence analysis was accepted as the standard method. Results BKV positivity was detected in 9 samples and JCV positivity in 61 samples by real-time PCR method specific to small t gene region; BKV positivity in 21 samples and JCV positivity in 67 samples were determined by real-time PCR method specific to the large T gene region. Statistically, there was a significant difference for BKV, but not significant difference for JCV detection between the two methods. Conclusion Different polymorphisms in the target gene regions were responsible for the different outcomes obtained from this study. With this sensitivity and specificity, in-house PCR method which we used is a candidate for routine diagnosis.Öğe Comparison of the GenoType® MTBC Molecular Genetic Assay with culture methods in the diagnosis of tuberculosis(Termedia Publishing House Ltd, 2014) Kurtoglu, Muhammet Guzel; Ozdemir, Mehmet; Kesli, Recep; Baysal, BulentIntroduction: Clinical samples from 433 patients pre-diagnosed with tuberculosis in Konya, Turkey, were investigated prospectively to compare the GenoType (R) MTBC test (GenoType (R) MTBC) with conventional gold standard culture methods. Material and methods: Lowenstein Jensen (LJ) and Mycobacteria Growth Indicator Tube (MGIT)-960 culture methods and GenoType (R) MTBC were performed together. Results: Mycobacterium tuberculosis (M. tuberculosis) detection rates were 16.2% by culture methods, 15.4% by GenoType (R) MTBC, and 6% by acid-fast bacilli microscopy. The LJ or MGIT-960 with GenoType (R) MTBC detected M. tuberculosis in 12 samples each that were negative according to the other culture method alone. Among 70 M. tuberculosis-positive samples, detection rates were 37% (26/70) by microscopy and 82.8% (58/70) by LJ and MGIT-960, but 95.7% (67/70) by GenoType (R) MTBC. Conclusions: GenoType (R) MTBC may be used as a beneficial adjunct test to culture methods for the detection of M. tuberculosis.Öğe Comparison of the performance of TK system with LJ and MGIT methods in the diagnosis of tuberculosis(E-Century Publishing Corp, 2014) Feyzioglu, Bahadir; Dogan, Metin; Sanli, Ozlem O.; Ozdemir, Mehmet; Baykan, MahmutTuberculosis is a common infectious disease caused by various strains of mycobacteria, usually Mycobacterium tuberculosis (TB). Various liquid or solid media are used for the diagnosis of tuberculosis. TK Rapid Mycobacterial Culture System has been developed recently. In our study, we aimed to compare TK Rapid Mycobacterial Culture System with LJ and MGIT systems in the diagnosis of tuberculosis. 200 clinical specimens (152 sputum, 41 Bronchoalveolar lavage fluid (BAL), 4 gastric aspirations, 2 urine and 1 wound) obtained from 192 patients from different clinics were included for the diagnosis of TB. All specimens were decontaminated by using the same-common procedure in all the methods. The obtained sediment was used for inoculation for the BACTEC MGIT 960, TK and LJ. Additionally, smears were prepared from the residual suspension for Ehrlich-Ziehl-Neelsen (EZN) staining for microscopic examination. Contamination was observed in 23 sputum and 4 BAL samples. Contamination rates for TK, LJ, and BACTEC MGIT 960 systems were determined as 3 (1.5%), 13 (6.5%), and 18 (9%) respectively. Mycobacterium tuberculosis growth was determined as 15 (7.5%), 14 (7%) and 13 (6.5%) by TK culture system, MGIT and LJ, respectively. In our study, the total mean detection times of Mycobacterium tuberculosis by the LJ, TK, and MGIT method were 20.1, 17.1, and 8.3 days, respectively. TK system showed a dramatically lower contamination rate than the others. There was no difference in growth rates for each of the three methods. We concluded that the TK culture system is disadvantageous in terms of turnaround time.Öğe COVID-19 Seroprevalance in a University Hospital Health Workers(Bilimsel Tip Yayinevi, 2021) Arslan, Gokce Kader; Ozdemir, Mehmet; Kaya, Havva; Feyzioglu, Bahadir; Kepenek Kurt, Esma; Erayman, IbrahimIntroduction: Healthcare workers are at the forefront in the Pandemic war against COVID-19 (Coronavirus Disease 2019) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2). In this struggle, they have become high-risk by keeping in close con- tact with patients during their diagnosis, treatment, and follow-up with long working hours. The aim of this study was to contribute to epidemiological data of our country by examining the antibody status of our hospital healthcare workers. Materials and Methods: Anti-SARS-CoV-2 IgG/IgM, COVID-19 ELISA kits were studied from sera samples of healthcare workers in Necmettin Erbakan University Meram Medical Faculty Hospital between June 1 and November 30, 2020. Nasopharyngeal swab sam- ples of these persons were also tested with the Real Time Polymerase Chain Reaction (RT-PCR) method. Results: SARS-CoV-2 seroprevalence of 741 healthcare workers included in our study was found to be 17%. Seropositivity was detected in 6.4% (33/515) of the healthcare workers with negative SARS-CoV-2 PCR test and in 3.9% (5/130) of the healthcare workers who did not have PCR test. Among the healthcare workers, the highest seroprevalence was observed in nurses (39.6%) followed by doctors (%23). Conclusion: It was evaluated that SARS-CoV-2 seroprevalence in healthcare workers is higher than in the population. This study shows that occupational exposure is a risk factor. 3.9% seropositivity was found in healthcare workers who never had a test. Considering that these workers have an asymptomatic or subclinical infection, there is a possible risk for nosocomial transmission. Therefore, healthcare professionals should use personal protective equipment and apply hygiene rules correctly and effectively in infectious diseases, especially during pandemic periods, while working in the hospital.Öğe Delta variant effect on the clinical course of adolescent COVID-19 patients(Wiley, 2023) Caglar, Hanife Tugce; Pekcan, Sevgi; Yilmaz, Asli Imran; Unal, Gokcen; Akcan, Ozge Metin; Unsacar, Mahmut Z.; Ozdemir, MehmetObjective The clinical course of new COVID-19 variants in adolescents is still unknown. The aim of this study is to evaluate the clinical characteristics of COVID-19 in adolescents and compare the differences between the original version and the delta variant. Materials and Methods The medical records of patients aged 10-18 years treated for COVID-19 between April 1, 2020 and March 31, 2022 were retrospectively reviewed. Patients were divided into four groups (asymptomatic, mild, moderate, and severe) for COVID-19 severity and into two groups according to the diagnosis date (first-second year). The primary endpoint of the study was hospital admission. Results The mean age of patients was 171.81 +/- 29.5 months, and most of them were males (n: 435, 53.3%). While the patient number was 296 (43.52%) in the first year of pandemic, it raised to 520 (54.11%) in the second year (p < 0.01). The severity of COVID-19 was mild in 667 (81.7%) patients. In the comparison of patients according to the diagnosis date (first-second years); the parameters of anosmia, ageusia, weakness, muscle pain, vomiting, hospital admission, and length of stay in hospital were statistically different (p < 0.05). In the comparison of hospitalized patients between years, the necessity of oxygen support (p < 0.001), endotracheal intubation rates (p < 0.05), length of stay in the hospital (p < 0.001), and the severity of COVID-19 (p < 0.05) was significantly higher in the second year. Conclusion The clinical course for adolescents diagnosed with COVID-19 has linearly changed with the delta variant. Our results confirmed that the delta variant is more transmissible, requires more oxygen support, increases endotracheal intubation, and prolongs the length of stay in the hospital.Öğe Detection of influenza viruses from patients in university hospital(Elsevier Science Bv, 2016) Gulcen, Begum Saran; Feyzioglu, Bahadir; Ozdemir, Mehmet; Baykan, Mahmut[Abstract Not Availabe]Öğe Detection of the frequency of PER-1 type extended-spectrum ?-lactamase-producing Acinetobacter baumannii clinical isolates in Turkey: a multicenter study(Tubitak Scientific & Technological Research Council Turkey, 2014) Asik, Gulsah; Ozdemir, Mehmet; Kurtoglu, Muhammet Guzel; Yagci, Server; Oksuz, Lutfiye; Gul, Mustafa; Kocoglu, Mucahide EsraBackground/aim: beta-Lactamases are an important resistance mechanism in Acinetobacter baumannii. Pseudomonas extended-resistance (PER-1) type beta-lactamase-producing strains have been reported from various geographic locations; however, PER-1 type beta-lactamases from Turkish hospitals have not been investigated extensively. The aim of this study was to determine the prevalence of PER-1 type beta-lactamases in A. baumannii isolates in various regions of Turkey. Materials and methods: A total of 763 clinical A. baumannii isolates were collected from 9 university hospitals and 2 state hospitals between 2008 and 2011. Molecular amplification of the OXA-51 gene from the A. baumannii genome was performed in order to verify identification of the species. Real-time polymerase chain reaction was used to detect bla(PER-1) genes. Results: PER-1 was detected in 24.6% of the isolates. The annual frequencies of the PER-1 enzyme were detected as 52.2%, 35.9%, and 8.3% in 2008, 2009, and 2010, respectively. PER-1 prevalence decreased gradually over time. The differences observed in PER-1 prevalence among the regions of Turkey were statistically significant (chi-square test; P < 0.001). Conclusion: These data demonstrate that the frequency of detection of PER-1 type beta-lactamases in A. baumannii species has decreased in Turkey. However, the increased carbapenem resistance, together with multidrug resistance, has created a worrisome situation regarding this pathogen.Öğe Determination of Epidemiology and Seasonal Distribution of Viral Agents Detected in Children with Respiratory Tract Infection(Aves Yayincilik, Ibrahim Kara, 2019) Tokak, Semih; Gulseren, Yasemin Derya; Ozdemir, MehmetObjective: The aim of this study was to determine the viral pathogens in the respiratory tract infections of children who applied to various outpatient clinics of our hospital and to investigate their seasonal distribution. Material and Methods: Between January 2016 and January 2017, 997 children (45.1% female, 54.9% male, 0 month-17 years) who were diagnosed with upper or lower respiratory tract infection were included in the study. Twenty-one viral respiratory pathogens were analyzed by multiplex polymerase chain reaction method by using Fast Track FTD kit (Fast Track Diagnosis, Luxemburg). Results: One or more respiratory viruses were detected in 761 (76.3%) of 997 patients and no virus was detected in 236 (22.8%) of the patients. In our study, distrubition of respiratory tract viruses were as; Adenovirus (2.76%), Bocavirus (4.20%), Coronavirus 229E (0.92%), Coronavirus OC43 (6.96%), Enterovirus (6.04%), Metapneumovirus A (4.60%), Metapneumovirus B (4.47%), Parainfluenza 1 (0.13%), Parainfluenza 2 (1.18%), Parainfluenza 3 (8.80%), Parainfluenza 4 (1.18%), Parainfluenza 4a (0.13%), Parainfluenza 4b (0.13%), Rhinovirus (48.75%), RSVA/B (37.84%), Influenza B (3.02%) and Parechovirus (6.57%). When we observe the seasonal distribution of viral agents, RSV was the most common agent in winter and it was rhinovirus in spring, summer and autumn season. Conclusion: Approximately 80% of the patients included in the study had a viral agent that may be responsible for clinical symptoms. For this reason, the rapid and sensitive diagnosis of viruses causing viral respiratory infections will reduce the cost of treatment, reduce unnecessary use of antibiotics and prevent the development of resistance to antibiotics and will guide the clinician to prevent the infections caused by these viruses.Öğe Distribution of blaOXA Genes in Acinetobacter baumannii Strains: A Multicenter Study(Ankara Microbiology Soc, 2013) Ciftci, Ihsan Hakki; Asik, Gulsah; Karakece, Engin; Oksuz, Lutfiye; Yagci, Server; Cetin, Emel Sesli; Ozdemir, MehmetAcinetobacter baumannii is the most important agent of nosocomial infections within the Acinetobacter genus. This gram-negative coccobacillus is intrinsically resistant to many antibiotics used in antimicrobial therapy, and capable of developing resistance including carbapenems. The objective of this study was to develop a multiplex real time polymerase chain reaction (qPCR) kit for OXA subgroups in A.baumannii, and to investigate the distribution of OXA subgroups in A.baumannii strains isolated from geographically different regions of Turkey. A total of 834 A.baumannii clinical isolates collected from different state and university medical centers in 13 provinces (Afyonkarahisar, Ankara, Bolu, Elazig, Erzurum, Isparta, Istanbul, Kahramanmaras, Konya, Sakarya, Van) between 2008-2011, were included in the study. The isolates were identified by conventional methods and automated systems [Vitek2 (bioMerieux, ABD) and Phoenix (BD Diagnostic, MD)]. The susceptibility profiles of the isolates were studied with automated systems and standard disc diffusion method. All samples were subjected to qPCR to detect bla(OXA-51-like), bla(OXA-23-like) and bla(OXA-58-like) genes. A conventional PCR method was also used to detect bla(OXA-24-like) gene. The resistance rates observed during the study period were as follows: 96.8% for amoxicillin-clavulanate, 86.8% for ciprofloxacin, 74.7% for gentamicin, 71.7% for amikacin, 73.5% for cefaperozone-sulbactam, 72.1% for imipenem and 73% for meropenem. Six hundred and two (72.2 %) isolates were resistant to both imipenem and meropenem. Colistin was found to be the most effective antibiotic against A.baumannii isolates with 100% susceptibility rate. All isolates were positive for bla(OXA-51-like) gene, however bla(OXA-24-like) gene could not be demonstrated in any isolate. Total positivity rates of bla(OXA-23-like) and bla(OXA-58-like) genes were found as 53.7% and 12.5%, respectively, while these rates were 74.4% and 17.3% in carbapenem-resistant isolates, respectively. Twenty-five isolates were positive-for both bla(OXA-23-like) and bla(OXA-58-like) genes. All of the carbapenem-resistant isolates have OXA type genes like with the exception of bla(OXA-24-like) gene. The positivity rates for bla(OXA-23-like) and bla(OXA-58-like) genes varied for each center. In addition, there was a decrease in the frequency of bla(OXA-58-like) gene, however both bla(OXA-23-like) gene and carbapenem resistance rates increased during the study period. In conclusion, high rates of resistance to carbapenems were also remarkable but A.baumannii strains keep on sensitivity to colistin. Both bla(OXA-23-like) and bla(OXA-58-like) genes were shown to be widespread in carbapenem-resistant A.baumannii clinical isolates. However, bla(OXA-23-like) gene positive strains were increased throughout the study. Currently, multiplex qPCR is the best way for rapid diagnosis of resistant bacteria for prevention of hospital-acquired infections. The multiplex qPCR kit developed in this study could be useful for rapid diagnosis and identify the frequencies of bla(OXA-23-like), bla(OXA-51-like) and bla(OXA-58-like) genes in carbapenem-resistant A.baumannii clinical isolates.Öğe Effects of melatonin on bacterial translocation in an experimental short bowel syndrome(Academic Journals, 2012) Akbaba, Soner; Isik, Sevil; Ozogul, Yusuf; Bostanci, Erdal Birol; Aydog, Gulden; Ozdemir, Mehmet; Atalay, FuatThe most important reasons for morbidity and mortality in short bowel syndrome (SBS) are septic complications. There is no specific treatment to prevent bacterial translocation (BT) which causes these complications. Melatonin is proven to have positive effects on the gastrointestinal system. The aim of this study was to evaluate the effects of exogenously administered melatonin on BT in SBS. Fifty Sprague-Dawley rats were divided into six groups. In control groups, the rats underwent laparotomy (Group I, Control + saline, n = 5), (Group II, Control + Melatonin, n = 5, 20 mg/kg, IM). In Sham groups, the rats underwent ileal transection (Group III, Sham + Saline, n = 8), (Group IV, Sham + Melatonin, n = 8). In SBS groups, the rats underwent 75% small bowel resection (Group V, SBS + Saline, n = 12), (Group VI, SBS + Melatonin, n = 12). Histopathological and microbiological studies were performed at the 3rd postoperative day. No difference was detected in sites of BT and colony numbers between the various groups. BT was more common in SBS groups than sham groups. However, melatonin administered rats were observed to have decreased the BT rates as compared to their control groups. The number of Kupffer cells in the liver decreased in the ongoing surgery groups while melatonin administration, on the other hand, increased Kupffer cells. Sinusoids were observed to expand due to the surgical operation, while melatonin administration increased parenchymal areas in mesenteric lymph nodes (MLN). In SBS + Melatonin group, there were increased villus heights and total mucosal thickness than those of the control and sham groups. This study demonstrated that melatonin can ensure protection against BT in SBS as it contributes to the maintenance of immune defensive mechanisms and increases intestinal adaptive response.Öğe The Efficiency of Hepatitis C Virus Core Antigen Test in the Diagnosis of Hepatitis C Infection(Galenos Yayincilik, 2016) Demircili, Mehmet Emin; Ozdemir, Mehmet; Feyzioglu, Bahadir; Baysal, BulentObjective: It was aimed to investigate diagnostic value of hepatitis C virus (HCV) core antigen test in patients with positive or negative anti-HCV assay by comparing with HCV ribonucleic acid (RNA) assay. Materials and Methods: Serum samples obtained from 189 patients who were admitted to Necmettin Erbakan University Meram Faculty of Medicine between December 2010 and February 2012, and HCV RNA assay were carried out for various reasons. Two mL of samples were stored under suitable conditions and anti-HCV, HCV core antigen and strip immunoblot assay [Commercial INNO LIA (TM) HCV Score (Innogenetics NV in Ghent, Belgium)] were performed. Genotyping was performed in the amplicons of the samples with positive HCV RNA test. Results: The diagnostic sensitivity specificity, negative predictive value and positive predictive value of HCV core antigen test were 96.2%, 100%, 97.3%, and 100%, respectively. Sixty-five serum samples were genotyped and their distribution were detected: Fifty-nine samples were genotype 1b, 2-genotype 1a/1b, 1-genotype 3a, 1-genotype 4, 1-genotype 2a/2c, and 1 was genotype 1a. Conclusion: It was concluded that HCV core antigen assay is highly specific, sensitive, reliable, reproducible, and easy to perform. It may be applied as a supplemental and confirmatory test in anti-HCV assays in the diagnosis of HCV.Öğe Frequency of Adenovirus and Rotavirus and Their Seasonal Distribution in Children With Gastroenteritis(Aves, 2016) Tuzuner, Ugur; Gulcen, Begum Saran; Ozdemir, Mehmet; Feyzioglu, BahadirObjective: In our study, we aimed to investigate the prevalence of rotavirus and enteric adenovirus in stool samples sent to our laboratory for antigen detection of children between 0-18 years of age admitted to hospital with diarrhea, abdominal pain, vomiting and fever and diagnosed as gastroenteritis. We also analyzed their frequencies according to demographic parameters. Methods: Results of 5156 pediatric patients admitted to Nec-mettin Erbakan University Meram Faculty of Medicine Hospital and diagnosed as gastroenteritis between January 2013-December 2015 were investigated retrospectively. VIKIA (R) Rota-Adeno (bioMerieux, Marcy l'Etoile, France), a chromatographic immunoassay detecting both viruses simultaneously was used according to the manufacturer's recommendations in stool samples. Results: Viral antigens were detected in 884 (17.1%) of the total 5156 samples. 764 (14.8%) of the positive results were detected as rotavirus and 120 (2.3%) were detected as adenovirus. Of the patients with positive results, 412 (46.6%) were female and 472 (53.4%) were male. When results are considered according to age, 2-4 age group was found to have the most common positivity (n=372) as 42.1%. Seasonal distribution of acute gastroenteritis cases was analyzed and the number of cases due to rotavirus was found to be increased in winter and spring and enteric adenoviruses were detected all year round. Conclusions: Rotavirus is the most common reason of gastroenteritis in the newborn and children, which must be considered for patients with diarrhea especially in the first four years of life. Rapid diagnosis is important for prediction of clinical implications and treatment. As enteric adenovirus is an important reason of gastroenteritis in infancy and childhood, it is necessary to investigate adenovirus antigens as well. Conducting regional studies are important for contributing to epidemiological data.Öğe Hepatitis C Genotypes in Patients with Chronic Hepatitis C Infection: A Three-Year Evaluation(Bilimsel Tip Yayinevi, 2020) Gulseren, Yasemin Derya; Esenkaya Tasbent, Fatma; Ozdemir, Mehmet; Feyzioglu, BahadirIntroduction: In case of chronic hepatitis C infection, cirrhosis and hepatocellular carcinoma may progress. HCV genotypes and subtypes have been found to vary according to geographical regions. In addition to its epidemiological importance, HCV genotype is an important factor in determining the response and duration of treatment. In this study, it was aimed to determine the genotype distribution in our region. Materials and Methods: The results of 241 patients with HCV RNA positivity detected in our laboratory Molecular unit between 2016 and 2018 were retrospectively screened. HCV-RNA extraction for genotyping was performed by automated system (EZ1 Virus Mini Kit v.2.0, Germany), and line probe assay (LIPA) based on reverse hybridization method was applied. HCV-RNA levels were determined by real-time PCR method (Artus HCV QS-RGQ kit, Qiagen, Germany). Results: Two hundred and forty-one patients were included in the study, and 116 (48%) were females and 125 (52%) were males. Mean age was 56.1 +/- 19.4 (range: 16-90) years. Mean logarithmic viral load value was 5.7 +/- 0.9 IU/ml (range; 2.71 x 10(2)-17 x 10(6)), mean value of AST was 50.5 +/- 43.7 IU/ml and mean ALT value was 63.4 +/- 63.5 IU/ml. Genotype 1b was detected in 58.9% of the patients, genotype 3a in 14.1%, genotype 1a in 13.27%, genotype 2b in 4.1%, genotype 4a in 1.2%. The subtypes could not be determined for 4.9%, 1.2%, 1.6% and 0.4% of infected patient in genotype 1,2,4 and 5 respectively. Conclusion: In our study, genotype 1b (58.9%) was found as the dominant genotype. This was followed by genotype 3a (14.1%). In patients infected with genotype 1, viral load value was found to be significantly higher than other genotypes. Monitoring genotype change is important for determining treatment protocols and duration.Öğe Infection Diseases Following Natural Disaster in Children: Health Prevention and Assessment(Georg Thieme Verlag Kg, 2023) Ugrakli, Selin; Ozdemir, Mehmet; Gray, James W.[Abstract Not Availabe]Öğe Investigation of Children with Acute Gastroenteritis by Multiplex PCR Method in Central Part of Turkey(Georg Thieme Verlag Kg, 2022) Yilmaz, Fatih; Kaya, Havva; Ozdemir, MehmetObjective Gastroenteritis is a disease that affects all age groups, especially children, and causes high mortality and morbidity in all countries. The most common agents of acute gastroenteritis are viral agents. As a result, millions of diarrhea attacks and hospital admissions occur worldwide every year due to viral gastroenteritis. This study uses the multiplex polymerase chain reaction (PCR) method to investigate the viruses that are the causative agents of viral gastroenteritis in the pediatric patient group in Konya, Turkey. Methods Stool samples of 94 patients aged 0 to 18 years sent from Emergency clinics and Pediatric outpatient clinics, Meram Medical Faculty Hospital Pediatric clinics, Konya Necmettin Erbakan University to Medical Microbiology Laboratory with a diagnosis of gastroenteritis between February and December 2018 were included in the study. Stool samples were stored at -80 degrees C until the time of the analysis. Deoxyribonucleic acid/ribonucleic acid isolation from stool samples was performed with EZ1 Virus Mini Kit v2.0 (Qiagen, Hilden, Germany) using an automatic extraction system (BioRobot EZ1 system, Qiagen). The presence of astrovirus, rotavirus, adenovirus, norovirus (GI, GII), and sapovirus agents was investigated by the multiplex PCR method (Fast Track Diagnostics, Luxembourg) viral gastroenteritis kit. Results Viral gastroenteritis agents were detected in 56.3% of the patients. One viral agent was detected in 47 (50%) of these patients and at least two viral agents in 6 (6.3%) of them. Norovirus GII was detected in 20 (21.2%) of the children included in the study, adenovirus in 13 (13.8%), rotavirus in 11 (12.8%), astrovirus in 11 (11.7%), sapovirus in 4 (4.2%), and norovirus GI in 1 (1.06%). When the distribution of viral agents was examined by months, the most number of agents were observed (21; 35%) in May, followed by April and June (12; 20%). Considering the distribution of the prevalence of the agents by age, it was seen to be mainly between 0 and 12 months (42%). Conclusion Considering that the most common viral agent in our region is norovirus GII, it will be useful to investigate the norovirus that is not routinely examined in children who are admitted to clinics with the complaint of gastroenteritis. It will be appropriate to examine routinely adenovirus, rotavirus, and norovirus in the laboratory, especially in children with diarrhea and vomiting in the winter and spring months.Öğe Investigation of Parvovirus B19 IgM and IgG Positivity Rates in Pediatric Hematology Patients(Georg Thieme Verlag Kg, 2018) Gorkem, Aysun; Ugur, Ayse Ruveyda; Ozdemir, Mehmet; Feyzioglu, Bahadir; Baykan, MahmutHuman parvovirus B19 is a frequent etiologic agent causing erythema infectiosum in children. It has recently been suggested that parvovirus B19 may be latent after infection and cause reactive infections especially in immunosuppressed patients with hematological problems. In this study, we aimed to investigate the parvovirus B19 immunoglobulin M (IgM) and immunoglobulin G (IgG) seropositivity rates of patients evaluated in a pediatric hematology clinic. We retrospectively screened the laboratory results of parvovirus B19 IgM and IgG antibody assays of children less than 18 years, who consulted pediatric in-and-outpatient clinics between 2013 and 2016. Parvovirus B19 IgM and IgG antibodies were investigated in serum samples by using enzymelinked immunosorbent assay method in the Medical Microbiology Laboratory. Parvovirus B19 IgM antibodies were detected in 109 of 602 patients attending pediatric hematology clinics (18.1%). Parvovirus B19 IgG antibody was detected in 244 of 952 patients attending pediatric hematology clinics (25.6%). Parvovirus B19 IgM and IgG positivity in samples from pediatric in-and-outpatient clinics other than pediatric hematology were 2.8% and 35.7%, respectively. Parvovirus IgM and IgG positivity in serum samples sent from the pediatric hematology clinic and outpatients was statistically significant compared with those sent from pediatric clinics other than pediatric hematology (p = 0.0001 and p = 0.0008, respectively). The higher detection rate of serum parvovirus B19 IgM positivity in patients under the follow-up of pediatric hematology clinics suggests that immune suppression-related viral reinfection or persistence may occur in these patients.Öğe INVESTIGATION OF PARVOVIRUS B19 SEROPREVALENCE IN VARIOUS AGE GROUPS IN CENTRAL ANATOLIA REGION, TURKEY(Ankara Microbiology Soc, 2010) Dagi, Hatice Turk; Ozdemir, Mehmet; Baykan, Mahmut; Baysal, BulentHuman parvovirus B19 is a small, non-enveloped, icosahedral symmetric, single-stranded DNA virus that can cause a number of diseases, notably erythema infectiosum in children and aplastic crisis in patients with chronic hemolytic disorders. There have been limited data on the epidemiological pattern of parvovirus B19 infection in Turkey. The objective of this study was to investigate the seroprevalence of parvovirus B19 in Konya province (Central Anatolia), Turkey. Parvovirus B19 IgG antibodies were investigated by a commercial ELISA kit (RIDASCREEN, R-Biopharm AG, Germany) in 631 adults (age range: 18-> 60 years) and 542 children (age range: 0-17 years). The overall prevalence of parvovirus B19 IgG antibodies was 28.9%. The rate of parvovirus B19 IgG positivity was 20.7% (112/542) in the 0-17 years age group and was 36% (227/631) in the adult population. No significant difference in seropositivity rates were detected in terms of sex in children and adult group (p>0.05 in both groups). The rates of parvovirus B19 IgG seropositivity were 15.8% in 0-4 years age group, 16% in 5-9 years, 24.2% in 10-14 years, 40.9% in 15-19 years, 34.7% in 20-29 years, 35.5% in 30-39 years, 32.2% in 40-49 years, 37.5% in 50-59 years and 53.8% in > 60 years age group. The seropositivity rates in 0-4 and 5-9 years age groups were lower than the other age groups and the difference was statistically significant (p < 0.05). To determine the prevalence of parvovirus B19 in different age groups in different geographical areas is necessary since this will provide important information about the epidemiology of such infections.
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