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Öğe Acute and subacute effects of thymoquinone on acute methanol intoxication: An assessment based on serum TBARS and BDNF levels in rat model(Marmara Univ, Fac Medicine, 2022) Kozak, Hasan Huseyin; Kilinc, Ibrahim; Ozkurkculer, AlpaslanObjectives: Previous studies have shown the role of oxidative stress in methanol (MeOH) neurotoxicity. In clinical practice ethanol (EtOH) was used for the treatment of MeOH intoxication. Treatment with EtOH results in depression of the central nervous system, which may occur even at therapeutic doses. It also induces oxidative stress. Antioxidant and neuroprotective effects of thymoquinone (TQ) are known in different models of neurotoxicity. There are no studies investigating the protective effect of TQ against acute MeOH intoxication. We aimed to evaluate the effect of TQ administration on serum thiobarbituric acid reactive substances (TBARS) and Brain-Derived Neurotrophic Factor (BDNF) levels in rats with experimentally-induced MeOH intoxication. Materials and Methods: Six groups were constituted. Methotrexate (Mtx) treatment (0.3 mg/kg/day) intraperitoneally (i.p.) was given for 7 days to slow down the formate metabolism of all rats except controls in order to create a MeOH intoxication similar to that in humans. On the 8th day of the experiment, 3 g/kg MeOH was injected i.p. in MeOH, EtOH and TQ groups. Four hours after MeOH administration, 0.5 g/kg EtOH was injected i.p. in EtOH group and 30 mg/kg TQ was administered i.p. in TQ1 and TQ2 groups. In addition, a total of 5 doses of 30 mg/kg TQ was injected i.p. 24, 48, 72 and 96 hours after the first dose in TQ2 group. Saline solution was given i.p. in the other groups. Blood samples were obtained for evaluating serum TBARS and BDNF levels. Results: The highest TBARS level was found in MeOH+MTx group and this increase was statistically significant as compared to control and Mtx groups (p<0.001) . A statistically significant reduction was detected in serum TBARS levels in MeOH+Mtx+EtOH, MeOH+Mtx+TQ1 and MeOH+Mtx+TQ2 groups (p<0.001). Maximum serum BDNF level elevation was found in MeOH+Mtx group and this increase was statistically significant as compared to control and Mtx groups (p<0.001). Serum BDNF levels were higher in MeOH+Mtx+EtOH, MeOH+Mtx+TQ1 and MeOH+Mtx+TQ2 groups and the difference was statistically significant (p<0.001). Conclusions: Thymoquinone could suppress proinflammation and lipid peroxidation in MeOH intoxication, lead to rapid toxicity adaptation, and play the role of neuroprotection more effectively than EtOH. These results may suggest that TQ could be used as an alternative treatment option in MeOH intoxication.Öğe Chronic immobilization stress induces anxiety-related behaviors and affects brain essential minerals in male rats(Hogrefe Ag-Hogrefe Ag Suisse, 2022) Sahin, Zafer; Ozkurkculer, Alpaslan; Kalkan, Omer Faruk; Ozkaya, Ahmet; Koc, Aynur; Koca, Raviye Ozen; Solak, HaticeAlterations of essential elements in the brain are associated with the pathophysiology of many neuropsychiatric disorders. It is known that chronic/overwhelming stress may cause some anxiety and/or depression. We aimed to investigate the effects of two different chronic immobilization stress protocols on anxiety-related behaviors and brain minerals. Adult male Wistar rats were divided into 3 groups as follows (n = 10/group): control, immobilization stress-1 (45 minutes daily for 7-day) and immobilization stress-2 (45 minutes twice a day for 7-day). Stress-related behaviors were evaluated by open field test and forced swimming test. In the immobilization stress-1 and immobilization stress-2 groups, percentage of time spent in the central area (6.38 +/- 0.41% and 6.28 +/- 1.03% respectively, p < 0.05) and rearing frequency (2.75 +/- 0.41 and 3.85 +/- 0.46, p < 0.01 and p < 0.05, respectively) were lower, latency to center area (49.11 +/- 5.87 s and 44.92 +/- 8.04 s, p < 0.01 and p < 0.01, respectively), were higher than the control group (8.65 +/- 0.49%, 5.37 +/- 0.44 and 15.3 +/- 3.32 s, respectively). In the immobilization stress-1 group, zinc (12.65 +/- 0.1 ppm, p < 0.001), magnesium (170.4 +/- 1.7 ppm, p < 0.005) and phosphate (2.76 +/- 0.1 ppm, p < 0.05) levels were lower than the control group (13.87 +/- 0.16 ppm, 179.31 +/- 1.87 ppm and 3.11 +/- 0.06 ppm, respectively). In the immobilization stress-2 group, magnesium (171.56 +/- 1.87 ppm, p < 0.05), phosphate (2.44 +/- 0.07 ppm, p < 0.001) levels were lower, and manganese (373.68 +/- 5.76 ppb, p < 0.001) and copper (2.79 +/- 0.15 ppm, p < 0.05) levels were higher than the control group (179.31 +/- 1.87 ppm, 3.11 +/- 0.06 ppm, 327.25 +/- 8.35 ppb and 2.45 +/- 0.05 ppm, respectively). Our results indicated that 7-day chronic immobilization stress increased anxiety-related behaviors in both stress groups. Zinc, magnesium, phosphate, copper and manganese levels were affected in the brain.Öğe Comparison of Effect of Two Immobilization Stress Protocols on Depression/Anxiety Behavior in Male Rats(Wiley, 2017) Ozkurkculer, Alpaslan[Abstract Not Availabe]Öğe Effect of Apelin on Anxiety Like Behaviour in Male Rats(Karger, 2021) Sak, Zeynep Caliskan; Kutlu, Selim; Ozkurkculer, Alpaslan; Koca, Raviye Ozen; Solak, Hatice; Gormus, Zisik Solak[Abstract Not Availabe]Öğe The Effect of Morphine Dependence and Morphine Withdrawal on Neuritin and Some Markers of Neurogenesis in the Rat Hippocampus(Wiley, 2023) Cimen, Yasin Ali; Yildiz, Ibrahim; Gunes, Canan Eroglu; Ozkurkculer, Alpaslan; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe Effect of Morphine Dependency on Apelinergic System in Rat Hippocampus(Wiley, 2022) Yildiz, Ibrahim; Cimen, Yasin Ali; Eroglu, Canan; Ozkurkculer, Alpaslan; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe Evaluation of Effects of Three Distinct Stress Protocols on Depression and/or Anxiety-like Behaviors in Female Rats(Wiley, 2017) Sahin, Zafer; Koc, Aynur; Koca, Raviye Ozen; Solak, Hatice; Ozkurkculer, Alpaslan; Gormus, Zulfikare Isik Solak; Kutlu, Selim[Abstract Not Availabe]Öğe Long-term metabolic cage housing increases anxiety/depression-related behaviours in adult male rats(Taylor & Francis Ltd, 2019) Sahin, Zafer; Solak, Hatice; Koc, Aynur; Koca, Raviye Ozen; Ozkurkculer, Alpaslan; Cakan, Pinar; Gormus, Zulfikare Isik SolakThere are several reports on unfavourable effects of metabolic cage housing on animal welfare mainly due to the characteristic structures of these cages such as single housing and grid flooring. This study was aimed to compare the effects of long-term metabolic cage housing and conventional housing (normal grouped housing in standard cages) on the anxiety/depression-like behaviours in male rats. Anxiety/depression-related behaviours were evaluated by use of forced swimming test and open field test. Swimming and climbing were significantly lower and immobility duration higher in the metabolic cage group. In the open field test, total distance, mean velocity, time spent in the central area, zone transition, grooming, and rearing scores were significantly lower in the metabolic cage. Moreover, serum corticosterone level was higher in the metabolic cage group. The results of the study indicate that long-term metabolic cage housing may cause an increase in the anxiety- and depression-related behaviours in male rats.
