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EFFICIENT IN VITRO BULBLET REGENERATION FROM IMMATURE ZYGOTIC EMBRYOS OF FRITILLARIA IMPERIALIS AND F. PERSICA
(Parlar Scientific Publications (P S P), 2016) Cakmak, Derya; Sancak, Cengiz; Karaoglu, Cuma; Aasim, Muhammad; Parmaksiz, Iskender; Ozcan, Sebahattin
In this study in vitro bulblet regeneration potential of immature zygotic embryo explants of Fritillaria imperialis L. and F. persica L. were investigated. Explants were cultured on Murashige and Skoog's (MS) media supplemented with 0.25-1.0 mg/l thidiazuron (TDZ), 0.25-0.5 mg/l alpha-naphthalene acetic acid (NAA), 1.0-4.0 mg/l 6-benzylaminopurine (BAP) and 0.5-2.0 mg/l kinetin (kin). Multiple bulblet regeneration from all culture media was recorded after 14 months of culture. The highest bulblet regeneration frequency was recorded on media containing TDZ and NAA combinations from F. imperialis. Maximum bulblets per explants from F. imperialis and F. persica were attained on media containing BAP and NAA, kinetin and NAA or TDZ-NAA respectively. Bulblets subjected to hardening showed longer roots of 8.19 cm in a medium containing agar and activated charcoal (AC) cultured at 23 degrees C and higher number of roots per bulblet in a medium solidified with gelrite. Rooted bulblets were directly transferred to pots and cultured under greenhouse conditions where plants developed new foliage and roots, and acclimatized successfully.