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    Coenzyme Q10 improves the survival, mesenteric perfusion, organs and vessel functions in septic rats
    (Elsevier France-Editions Scientifiques Medicales Elsevier, 2017) Ozer, Erdem Kamil; Goktas, Mustafa Tugrul; Kilinc, Ibrahim; Pehlivan, Sultan; Bariskaner, Hulagu; Ugurluoglu, Ceyhan; Iskit, Alper Bektas
    Background: Coenzyme Q10 (CoQ10) is a naturally occurring, lipid-soluble antioxidant and an essential electron carrier in the mitochondrial respiratory chain. In sepsis, CoQ10 deficiency induced by mitochondrial failure can lead to hypoxia, hypoperfusion, oxidative organ damage and finally death. We aimed to investigate the effects of CoQ10 on survival, mesenteric artery blood flow (MABF), vascular reactivity, oxidative and inflammatory injuries in cecal ligation and puncture (CLP)-induced sepsis. Methods: Wistar rats were divided into Sham, CLP, Sham + CoQ10, CLP + CoQ10 subgroups. CoQ10 (10 mg/kg/day) or vehicle (olive oil; 1 mL/kg/day) was intraperitoneally injected for 15 days. At 16th day, Sham or CLP operation was performed. 20 h after the operations, MABF and phenylephrine responses of isolated aortic rings were measured. Tissue samples were obtained for histopathological and biochemical evaluations. Furthermore, survival rates were monitored throughout 96 h. Results: CoQ10 prevented mesenteric hypoperfusion and aortic dysfunction induced by CLP. Survival rate was % 0 at 46th h in CLP group, but in CLP + CoQ10 group it was 37.5% at the end of 96 h. CLP-induced elevations of serum AST, ALT, LDH, BUN, Cr and inflammatory cytokine (tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6) levels were blocked by CoQ10. CoQ10 restored the increased liver, lung, spleen and kidney malondialdehyde levels and as well as reduced liver and spleen glutathione levels. The protective effects of CoQ10 on multiple organ damage were also observed histopathologically. Conclusions: CoQ10 showed protective effects in sepsis due to its preservative effects on mesenteric perfusion, aortic function and also its anti-inflammatory and antioxidative effects. (C) 2017 Elsevier Masson SAS. All rights reserved.
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    Thymoquinone protects against the sepsis induced mortality, mesenteric hypoperfusion, aortic dysfunction and multiple organ damage in rat
    (Polish Acad Sciences Inst Pharmacology, 2017) Ozer, Erdem Kamil; Goktas, Mustafa Tugrul; Toker, Aysun; Pehlivan, Sultan; Bariskaner, Hulagu; Ugurluoglu, Ceyhan; Iskit, Alper Bektas
    Background: Thymoquinone (TQ) is a potent cytoprotective, antioxidant and anti-inflammatory agent. We aimed to investigate the possible protective effects of TQ on survival, mesenteric artery blood flow (MABF), vascular reactivity, oxidative and inflammatory injuries in a murine sepsis model induced by cecal ligation and puncture (CLP).Methods: Wistar rats were divided into the following four groups: Sham, CLP, Sham + TQand CLP + TQ. TQ (1 mg/kg/day) or vehicle (dimethyl sulfoxide, 1 mL/kg/day) was intraperitoneally injected for 3 days. At 4th day Sham or CLP operation was applied. 20 h after the operations, MABF and contractile responses of isolated aortic rings to phenylephrine were measured. Tissue samples were obtained for histopathological and biochemical examinations. Also, survival rates were recorded throughout 96 h.Results: TQ ameliorated mesenteric hypoperfusion and partially attenuated aortic dysfunction induced by CLP. Survival rate was %0 at 42nd h in CLP group, but in CLP + TQ group it was 33.4% at the end of 96 h. Serum levels of AST, ALT, LDH, BUN, Cr and inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6) increased in CLP group that were prevented by TQ. The decreases in liver, spleen and kidney glutathione levels and the increases in liver, lung, kidney and spleen malondialdehyde levels induced by CLP were inhibited by TQ. The histopathological protective effects of TQ on multiple organ damage due to CLP were also observed.Conclusion: TQ has ameliorative effects on sepsis due to its protective effects on mesenteric perfusion, contractile function of aorta and its anti-inflammatory and antioxidative effects. (C) 2017 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Sp. z o.o. All rights reserved.