Yazar "Sadi, Gokhan" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    IN VITRO CYTOTOXICITY, ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF VARIOUS EXTRACTS FROM SCHIZOPHYLLUM COMMUNE FR.
    (Parlar Scientific Publications (P S P), 2017) Emsen, Bugrahan; Kocabas, Aytac; Kaya, Abdullah; Cinar, Seval; Aasim, Muhammad; Sadi, Gokhan
    Mushrooms have become attractive materials as a source of physiologically valuable bioactive compounds recently. This study was designed to investigate antioxidant, antibacterial and cytotoxic properties of Schizophyllum commune Fr. which is highly distributed worldwide. Five different extracts of S. commune were obtained with different solvent systems. Their antioxidant potentials were determined by measuring bioactive components (beta-carotene, flavonoids, lycopene and total phenols) in conjunction with radical scavenging and metal chelating activities as well as its reducing powers. Antibacterial activities of aqueous and methanol extract were observed on Bacillus subtilis (Ehrenberg) Cohn and Agrobacterium tumefaciens Smith & Townsend. In vitro cytotoxic effects of the extracts against liver hepatocellular carcinoma (HepG2) cell lines after 48 and 72 h incubation were tested. It was found that acetone extract of S. commune had maximum amount of beta-carotene (22.69 mu g/mg), phenolic (27.66 mu g/mg) and lycopene (8.73 mu g/mg) content and n-hexane extract had maximum amount of flavonoid (3.48 mu g/mg). According to IC50 values, the chloroform extract of S. commune showed the highest DPPH capturing (7.652 mg/ml) and metal chelating (6.590 mg/ml) activities. Furthermore, acetone and n-hexane extracts exerted substantial in vitro cytotoxic effects against liver hepatocellular carcinoma (HepG2) cell lines after 72 h incubation with IC50 value of 0.825 and 0.316 mg/ml respectively. Results of this study showed that different extracts of investigated mushroom have considerable cytotoxic and antioxidant properties and may be utilized as a promising source of therapeutics.
  • Küçük Resim Yok
    Öğe
    A tetraoxacalix[2]arene[2]triazine based fluorogenic probe for the sensing of Fe3+: Computational and living-cell imaging applications
    (Elsevier Science Sa, 2020) Karuk Elmas, Sukriye Nihan; Gunay, Ibrahim Berk; Genc, Hayriye Nevin; Aydin, Duygu; Arslan, Fatma Nur; Sadi, Gokhan; Sirit, Abdulkadir
    Herein, we have reported a feasibility study of highly selective and sensitive fluorogenic chemosensor (probe TOCT) derived from tetraoxacalix [2] arene [2] triazine for Fe3+ sensing in aqueous media. The probe TOCT has been fabricated and verified by H-1-NMR, HR-LCMS,FT-IRand C-13-NMR. The sensing properties of probe TOCT toward various metal ions were confirmed by fluorescence titration study as well as competitive study. The intensity of probe TOCT reduced with the amount of Fe3+ when it was excited at 300 nm in CH3CN:H2O (95/5, v/v, pH = 7) media. The probe demonstrated a 'turn-off' response toward Fe3+, in the presence of possible competing ions. The detection limit value of probe TOCT for Fe3+ sensing was computed to be 12.5 nM. The possible molecular structure of complex between probe TOCT and Fe3+ was also proposed on the basis of HR-LCMS and FT-IR. The stoichiometry of TOCT-Fe3+ complex was identified to be 1:1 to the methodology of Job's plot. The binding ability was studied through the equation of Benesi-Hildebrand which was computed to be 8.90 x 10(4) M-1 for Fe3+. To support the intensity enhancement and quenching studies, we reported computational experiments based on the density functional theory (DFT). Moreover, a great potential has been detected for probe TOCT as a fluorescent chemosensor in biological applications to monitor Fe3+ in living cells.