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Öğe Chronic immobilization stress induces anxiety-related behaviors and affects brain essential minerals in male rats(Hogrefe Ag-Hogrefe Ag Suisse, 2022) Sahin, Zafer; Ozkurkculer, Alpaslan; Kalkan, Omer Faruk; Ozkaya, Ahmet; Koc, Aynur; Koca, Raviye Ozen; Solak, HaticeAlterations of essential elements in the brain are associated with the pathophysiology of many neuropsychiatric disorders. It is known that chronic/overwhelming stress may cause some anxiety and/or depression. We aimed to investigate the effects of two different chronic immobilization stress protocols on anxiety-related behaviors and brain minerals. Adult male Wistar rats were divided into 3 groups as follows (n = 10/group): control, immobilization stress-1 (45 minutes daily for 7-day) and immobilization stress-2 (45 minutes twice a day for 7-day). Stress-related behaviors were evaluated by open field test and forced swimming test. In the immobilization stress-1 and immobilization stress-2 groups, percentage of time spent in the central area (6.38 +/- 0.41% and 6.28 +/- 1.03% respectively, p < 0.05) and rearing frequency (2.75 +/- 0.41 and 3.85 +/- 0.46, p < 0.01 and p < 0.05, respectively) were lower, latency to center area (49.11 +/- 5.87 s and 44.92 +/- 8.04 s, p < 0.01 and p < 0.01, respectively), were higher than the control group (8.65 +/- 0.49%, 5.37 +/- 0.44 and 15.3 +/- 3.32 s, respectively). In the immobilization stress-1 group, zinc (12.65 +/- 0.1 ppm, p < 0.001), magnesium (170.4 +/- 1.7 ppm, p < 0.005) and phosphate (2.76 +/- 0.1 ppm, p < 0.05) levels were lower than the control group (13.87 +/- 0.16 ppm, 179.31 +/- 1.87 ppm and 3.11 +/- 0.06 ppm, respectively). In the immobilization stress-2 group, magnesium (171.56 +/- 1.87 ppm, p < 0.05), phosphate (2.44 +/- 0.07 ppm, p < 0.001) levels were lower, and manganese (373.68 +/- 5.76 ppb, p < 0.001) and copper (2.79 +/- 0.15 ppm, p < 0.05) levels were higher than the control group (179.31 +/- 1.87 ppm, 3.11 +/- 0.06 ppm, 327.25 +/- 8.35 ppb and 2.45 +/- 0.05 ppm, respectively). Our results indicated that 7-day chronic immobilization stress increased anxiety-related behaviors in both stress groups. Zinc, magnesium, phosphate, copper and manganese levels were affected in the brain.Öğe Does Phosphodiesterase Inhibiting Agent-Milrinone Prevent Negative Inotropic Effects of Sertraline on Postoperative Human Heart Muscle Contractility? An In Vitro Experimental Study(Wiley, 2017) Gormus, Zulfikare Isik Solak; Koca, Raviye Ozen; Koc, Aynur; Solak, Hatice; Ozdemir, Ayse; Sahin, Zafer; Kutlu, Selim[Abstract Not Availabe]Öğe Effect of Intravenous Glucose Administration on Catecholamine Concentrations in Hypothalamic Ventromedial Nucleus in Rats: A Microdialysis Study(Wiley-Blackwell, 2016) Solak, Hatice; Koca, Raviye Ozen; Gormus, Z. Isik Solak; Sahin, Zafer; Koc, Aynur; Karaibrahimoglu, Adnan; Kutlu, Selim[Abstract Not Availabe]Öğe Effect of kisspeptin on calcium signalling in cultured rHypoE-8 cell line(Wiley-Blackwell, 2015) Kutlu, Selim; Ozdemir, Gokcen; Sahin, Zafer; Ozcan, Mete; Canpolat, Sinan; Kelestimur, Haluk[Abstract Not Availabe]Öğe The Effect of Phenoxybenzamine to Decreased Heart Muscle Contractility After in vitro Constituted Ischemia(Wiley-Blackwell, 2016) Gormus, Z. Isik Solak; Solak, Hatice; Koca, Raviye Ozen; Sahin, Zafer; Kutlu, Selim; Gormus, Niyazi[Abstract Not Availabe]Öğe Effect of Pregnancy and Lactation Period on Depression Like Behaviour in Depressive Rats(Karger, 2018) Solak, Hatice; Koca, Raviye Ozen; Koc, Aynur; Sahin, Zafer; Uguz, Faruk; Ak, Mehmet; Kurar, Ercan[Abstract Not Availabe]Öğe Effect of RFRP-3 on Anxiety-like Behaviour and Hippocampal Neurogenesis in Rats(Wiley-Blackwell, 2016) Boyukcam, Yasemin; Sahin, Zafer; Koc, Aynur; Eroglu, Canan; Gormus, Isik Solak; Kurar, Ercan; Kutlu, Selim[Abstract Not Availabe]Öğe Effects of Naringenin on Oxidative Stress and Histopathological Changes in the Liver of Lead Acetate Administered Rats(Wiley, 2016) Ozkaya, Ahmet; Sahin, Zafer; Dag, Uzeyir; Ozkaraca, MustafaLead has several adverse effects on the body due to one of the environmental pollutants. We aimed to determine the effects of naringenin on the oxidative stress and the hepatic damage against lead acetate treatment in the liver of male rats. Naringenin was administered by orogastric gavage (50 mg/kg) and lead acetate was given as daily 500 parts per million in drinking water for 4 weeks. Lead and antioxidant activities were measured, and histopathological evaluation was performed in the liver. Lead concentrations, malondialdehyde, and antioxidant activity were restored by the naringenin. The grade of necrosis, hydropic degeneration, and hepatic cord disorganization was decreased by the naringenin. However, there were no differences in the degree of sinusoidal congestion, hepatic steatosis, and capsular fibrosis between lead acetate and naringenin + lead acetate groups. We can suggest that naringenin has antioxidant and chelating effects in the liver. Nevertheless, this effect is not enough against the lead acetate induced hepatic injury. (C) 2016 Wiley Periodicals, Inc.Öğe Effects of Phenoxybenzamine after Hypothermic and Normothermic Ischemia Injury in Rat Aorta(Wiley-Blackwell, 2016) Gormus, Isik Solak; Koca, Raviye Ozen; Solak, Hatice; Sahin, Zafer; Karaibrahimoglu, Adnan; Gormus, Niyazi; Kutlu, Selim[Abstract Not Availabe]Öğe Evaluation of Apoptosis Pathway of Geraniol on Ishikawa Cells(Routledge Journals, Taylor & Francis Ltd, 2021) Kuzu, Betul; Cuce, Gokhan; Ayan, Ilknur Cinar; Gultekin, Burcu; Canbaz, Halime Tuba; Dursun, Hatice Gul; Sahin, ZaferEndometrial cancer is the most common type of cancer in the female reproductive system. Geraniol is acyclic monoterpene alcohol derived from essential oils of aromatic plants. This study aimed to investigate the apoptosis pathway of geraniol on Ishikawa cells. The cytotoxic effects of Geraniol on Ishikawa cells were determined by an MTT test. Ishikawa cells were seeded on cover slips, the IC50 dose was applied, and the cells were incubated with antibodies against Bax, Bcl-2, and TUNEL Assay. mRNA expression analysis of apoptosis-related genes was determined by RT-qPCR with an IC50 dose of Geraniol. The IC50 dose of Geraniol decreased Bcl-2 staining significantly, but it significantly increased Bax staining and TUNEL positive cells. A significant increase in the Bax, caspase3, caspase-8, cytochrome C and Fas genes and a significant decrease in the Bcl-2 gene was observed when the IC50 dose group was compared to the cells in the control group based on their mRNA expression levels.Analysis of expression of genes whose products are involved in apoptosis suggests the involvement of the mitochondrial pathway.Öğe Evaluation of Effects of Three Distinct Stress Protocols on Depression and/or Anxiety-like Behaviors in Female Rats(Wiley, 2017) Sahin, Zafer; Koc, Aynur; Koca, Raviye Ozen; Solak, Hatice; Ozkurkculer, Alpaslan; Gormus, Zulfikare Isik Solak; Kutlu, Selim[Abstract Not Availabe]Öğe Evaluation of Naringenin Chelating and Hepatoprotective Potential against to Lead Acetate Administration(Wiley-Blackwell, 2015) Sahin, Zafer; Ozkaya, Ahmet; Dag, Uzeyir; Ozkaraca, Mustafa[Abstract Not Availabe]Öğe Geraniol attenuates hydrogen peroxide-induced liver fatty acid alterations in male rats(Ejmanager Llc, 2017) Ozkaya, Ahmet; Sahin, Zafer; Gorgulu, Ahmet Orhan; Yuce, Abdurrauf; Celik, SaitBackground: Hydrogen peroxide (H2O2) is an oxidant agent and this molecule naturally occurs in the body as a product of aerobic metabolism. Geraniol is a plant-derived natural antioxidant. The aim of this study was to determine the role of geraniol on hepatic fatty acids alterations following H2O2-induced oxidative stress in male rats. Methods: After randomization, male Wistar rats were divided into four groups (n = 7 each group). Geraniol (50 mg/kg, dissolved in corn oil) and H2O2 (16 mg/kg, dissolved in distilled water) were administered by an intraperitoneal injection. Administrations were performed during 30 days with 1-day interval. Results: Administration of H2O2 resulted with a significant increase in malondialdehyde (MDA) and a significant decrease in glutathione (GSH) peroxidase glutathione level; geraniol restored its effects on liver. However, hepatic catalase (CAT) activities were significantly higher in H2O2, geraniol, and geraniol+ H2O2 groups than control group. The ratio of hepatic total saturated fatty acids increased in H2O2-treated animals compared with control. In addition, hepatic total unsaturated fatty acids reduced in H2O2 group compared with control. The percentages of both hepatic total saturated and unsaturated fatty acids were not different between geraniol+ H2O2 and control groups. Conclusions: H2O2-induced oxidative stress may affect fatty acid composition in liver and body. Geraniol can partly restore oxidative hepatic damage because it cannot completely reverse the H2O2 -induced increase in hepatic CAT activities. Moreover, this natural compound can regulate hepatic total saturated and unsaturated fatty acids percentages against H2O2-induced alterations.Öğe Hypothalamic Expressions of Apelin, Apelin Receptor and Neuritine in the First Generation Rat Pups: A Maternal Depression Model(Karger, 2021) Kurar, Ercan; Gunes, Canan Eroglu; Koca, Raviye Ozen; Solak, Hatice; Koc, Aynur; Sahin, Zafer; Cuce, Gokhan[Abstract Not Availabe]Öğe Investigation of Effect of Intravenous Glucose Administration on Noradrenaline Levels in Lateral Hypothalamic Area by using Microdialysis Method in Rat(Wiley-Blackwell, 2015) Solak, Hatice; Koca, Raviye Ozen; Gormus, Zulfikare Isik Solak; Sahin, Zafer; Koc, Aynur; Kutlu, Selim[Abstract Not Availabe]Öğe Investigation of Effects of Dimethyl Sulphoxide on Smooth Muscle Contractions in Rat Tissues in Isolated Organ Baths(Wiley, 2017) Solak, Hatice; Taki, Fatma Nur; Koc, Aynur; Koca, Raviye Ozen; Gormus, Z. Isik Solak; Sahin, Zafer; Kutlu, Selim[Abstract Not Availabe]Öğe Investigation of Some Nutrition-related Neuropeptide Expressions in Hypothalamus Tissues of Offspring Exposed to Maternal Depression and Sertraline Treatment.(Wiley, 2023) Solak, Hatice; Koca, Raviye Ozen; Gunes, Canan Eroglu; Uguz, Faruk; Ak, Mehmet; Sahin, Zafer; Kurar, Ercan[Abstract Not Availabe]Öğe Investigation of the effect of naringenin on oxidative stress-related alterations in testis of hydrogen peroxide-administered rats(Wiley, 2017) Sahin, Zafer; Ozkaya, Ahmet; Cuce, Gokhan; Uckun, Mirac; Yologlu, ErtanTestis tissue is prone to oxidation because its plasma membrane contains many polyunsaturated fatty acids. Naringenin is a plant-derived natural flavonoid. We investigated the possible ameliorative role of naringenin on the hydrogen peroxide (H2O2)-induced testicular damage in Wistar rats. Animals received 12mg/kg H2O2 by intraperitoneal injection, and 50mg/kg naringenin via orogastric gavage for 4 weeks. In the H2O2 group, the testis malondialdehyde level increased, while the amount of reduced glutathione, glutathione transferase activities, and the testis weight decreased. There were severe testicular damages in the H2O2 group otherwise their grade were less in the naringenin+H2O2 group. However, the serum testosterone concentrations decreased in both the H2O2 and the naringenin+H2O2 groups. The testicular zinc and calcium levels reduced in the H2O2-treated rats. In conclusion, the administration of H2O2 caused oxidative stress in the testes and naringenin supplementation decreased the H2O2-induced effects, except for changes in testosterone levels.Öğe Investigation of the effect of trazodone on spontaneous and acetylcholine-induced bladder detrusor smooth muscle contractions(Kare Publ, 2022) Burunsuz, Ozge; Ozdengul, Faik; Gica, Sakir; Sen, Aysu; Sahin, ZaferObjective: The management of psychiatric patients is often complicated by medical comorbidities, complex pharmacological regimens, and side effects secondary to these regimens. In the current study, it was aimed to investigate the effects and mechanism of action of trazodone on rat bladder smooth muscle contractility in vitro. Method: Sixteen adult male Wistar albino rats were euthanized by the cervical dislocation method following ether anesthesia. Two muscle strips of 2x10 mm in size were prepared vertically from the bladder by opening a longitudinal incision from the neck of the bladder in the direction of the apex. The strips were tied properly and hung in the organ bath. All contraction amplitudes and frequencies were recorded. After a 45-min adaptation period, contractions were induced by applying 10(-5) M acetylcholine (ACh) to all spontaneously contracting bladder strips. After 20 min, doses of trazodone (10(-9) M vs 10(-3) M) were given cumulatively. The resulting effects were recorded. Results: The groups were compared within themselves: a significant difference was found between the initial tensions in the group with ACh-induced contractions and the tensions after the administration of trazodone at 10(-9), 10(-8), 10(-7), 10(-6) , 10(-5), 10(-4), and 10(-3) M doses (p<0.0001). In the group with spontaneous contractions, a significant difference was found between the initial tensions and the tensions after the administration of trazodone at 10(-9), 10(-8), 10(-5), and 10(-4) M doses (p<0.0001). Conclusion: Results showed that trazodone had a significant inhibitory effect on bladder smooth muscle contractions in vitro, especially at concentrations of 10(-4) and 10(-3) M.Öğe Investigation of the effect of trazodone on spontaneous and acetylcholine-induced bladder detrusor smooth muscle contractions(Kare Publ, 2022) Burunsuz, Ozge; Ozdengul, Faik; Gica, Sakir; Sen, Aysu; Sahin, ZaferObjective: The management of psychiatric patients is often complicated by medical comorbidities, complex pharmacological regimens, and side effects secondary to these regimens. In the current study, it was aimed to investigate the effects and mechanism of action of trazodone on rat bladder smooth muscle contractility in vitro. Method: Sixteen adult male Wistar albino rats were euthanized by the cervical dislocation method following ether anesthesia. Two muscle strips of 2x10 mm in size were prepared vertically from the bladder by opening a longitudinal incision from the neck of the bladder in the direction of the apex. The strips were tied properly and hung in the organ bath. All contraction amplitudes and frequencies were recorded. After a 45-min adaptation period, contractions were induced by applying 10(-5) M acetylcholine (ACh) to all spontaneously contracting bladder strips. After 20 min, doses of trazodone (10(-9) M vs 10(-3) M) were given cumulatively. The resulting effects were recorded. Results: The groups were compared within themselves: a significant difference was found between the initial tensions in the group with ACh-induced contractions and the tensions after the administration of trazodone at 10(-9), 10(-8), 10(-7), 10(-6) , 10(-5), 10(-4), and 10(-3) M doses (p<0.0001). In the group with spontaneous contractions, a significant difference was found between the initial tensions and the tensions after the administration of trazodone at 10(-9), 10(-8), 10(-5), and 10(-4) M doses (p<0.0001). Conclusion: Results showed that trazodone had a significant inhibitory effect on bladder smooth muscle contractions in vitro, especially at concentrations of 10(-4) and 10(-3) M.
