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Öğe Antibacterial and Smear Layer Removal Efficacy of Er:YAG Laser Irradiation by Photon-Induced Photoacoustic Streaming in Primary Molar Root Canals: A Preliminary Study(Mary Ann Liebert, Inc, 2018) Korkut, Emre; Torlak, Emrah; Gezgin, Onur; Ozer, Hazal; Sener, YagmurObjective: The objective of this study was to compare antibacterial and smear layer removal efficacy of the erbium:yttrium-aluminium-garnet (Er:YAG) laser irradiation by photon-induced photoacoustic streaming (PIPS), neodymium:yttrium-aluminium-garnet (Nd:YAG), and diode lasers and conventional irrigation agent in primary molar root canals. Background: The selection, application, and activation of the irrigant are important factors affecting the success of the endodontic treatment. Materials and methods: Distal root canals of human primary mandibular molar teeth used for antimicrobial efficacy evaluation were first inoculated with Enterococcus faecalis (n=25). Smear layer removal efficacy was evaluated on the noninoculated specimens (n=20). The root canals in the first treatment groups were irrigated with a conventional technique using 5% sodium hypochlorite (NaOCl). In the laser groups, the root canals were irradiated with either Nd:YAG (1064nm) or diode lasers (940nm) followed by NaOCl irrigation agent. In the fourth groups, the NaOCl irrigation agent was activated with an Er:YAG laser (2940nm) by PIPS tip using nonablative settings. Antibacterial efficacy was determined by viable counts of E. faecalis after treatments. The treated root canals were examined by scanning electron microscopy to determine the smear layer removal efficacy of treatments. Results: Statistically significant (p<0.05) reductions in the number of E. faecalis were achieved in Nd:YAG and diode laser groups and Er:YAG laser by PIPS-activated irrigation groups compared to the NaOCl group. Er:YAG laser by PIPS-activated irrigation resulted in more cleaning of the root canal walls and a higher quantity of open tubules. Conclusions: These findings suggest that Er:YAG laser by PIPS-activated irrigation can be considered as an effective irrigant agitation technique in pediatric endodontics.Öğe Biofilm formation by Staphylococcus aureus isolates from a dental clinic in Konya, Turkey(Elsevier Science London, 2017) Torlak, Emrah; Korkut, Emre; Uncu, Ali T.; Sener, YagmurThe ability of Staphylococcus aureus to form biofilm is considered to be a major virulence factor influencing its survival and persistence in both the environment and the host. Biofilm formation in S. aureus is most frequently associated with production of polysaccharide intercellular adhesion by ica operon-encoded enzymes. The present work aimed at evaluating the in vitro biofilm production and presence of the icaA and icaD genes in S. aureus isolates from a dental clinic in Konya, Turkey. The surfaces of inanimate objects were sampled over a period of six months. S. aureus isolates were subjected to Congo Red Agar (CRA) and crystal violet (CV) staining assays to evaluate their ability of biofilm production, while the presence of the icaA and icaD genes was determined by polymerase chain reaction. S. aureus contamination was detected in 13.2% of the environmental samples. All the 32 isolates were observed to be positive for both the icaA and icaD genes. Phenotypic evaluations revealed that CV staining assay is a more reliable alternative to CRA assay to determine biofilm formation ability. A high percentage of agreement (91%) was observed between the results from CV staining and ica genes' detection assays. Phenotypic and genotypic evaluations should be combined to detect biofilm formation in S. aureus. Our findings indicate that dental clinic environments should be considered as potential reservoir for biofilm-producing S. aureus and thus cross contamination. (C) 2017 The Authors. Published by Elsevier Limited.Öğe Effect of different surface treatments on the shear and microtensile bond strength of resin-modified glass ionomer cement to dentin(Taylor & Francis Ltd, 2014) Altunsoy, Mustafa; Botsali, Murat Selim; Korkut, Emre; Kucukyilmaz, Ebru; Sener, YagmurObjective. The aim of this study was to evaluate the effect of different surface treatments on the microtensile bond strength (mTBS) and shear bond strength (SBS) of resin-modified glass ionomer cement (RMGIC) to dentin. Materials and methods. Fifty-two extracted human molars were flattened to obtain dentin surfaces. For SBS assessment 40 teeth were divided into four groups according to their surface treatments (acid etching, Er: YAG laser QSP mode, Er: YAG laser MSP mode and control-SiC) (n = 10). A plastic cylinder was placed over the differently treated dentin surfaces and RMGIC was placed into the rings and polymerized. Twelve teeth were used for the mu TBS test. The treated dentin surfaces described above were restored with 4 mm high RMGIC and light cured; then, the specimens were sectioned into serial sticks (n = 15) and mu TBS and SBS were tested for failure in a testing machine with a 1 mm/min crosshead speed. The data were analyzed by one-way ANOVA and Tukey HSD tests (alpha = 0.05). Results. Acid etching showed significantly higher SBS than the other groups (p < 0.05). Er: YAG QSP and MSP-treated groups showed higher SBS values than the control group, but the difference was not statistically significant (p > 0.05). Er: YAG MSP showed the highest mu TBS value followed by acid etching, whereas the control group exhibited the lowest value (p < 0.05) and the differences between the control group and Er: YAG QSP were not significant (p > 0.05). Conclusions. The application of Er: YAG MSP mode and acid etching to dentin can be used for improving the bond strength of RMGIC.Öğe HPLC analysis of eluted monomers released from dental composites containing bioactive glass(Taylor & Francis Ltd, 2018) Gezgin, Onur; Korkut, Emre; Tulumbaci, Fatih; Ozer, Hazal; Sener, YagmurObjectives: The aim of the present study is to evaluate the released residual monomers from composite resins that contain different proportions of bioactive glass (BAG). Methods: Experimental resin composites were prepared by a resin matrix (50% BisGMA and 50% TEGDMA) and inorganic filler with BAG (5, 10 and 30%). Each resin composite was placed in the tooth cavity (n=5). After polymerisation, samples were immediately immersed in 75% ethanol and 25% deionised water (6ml) at 37 degrees C. Residual monomers (Bis-GMA, TEGDMA, HEMA and UDMA) that were eluted from the composites for 10m, 1h, 1 d, 7 d and 30 d were analysed by high-performance liquid chromatography (HPLC). The data were analysed with one-way ANOVA and Tukey HSD at a p<0.05 significance level. Results: Among the time periods, the fastest released residual monomer was observed in the 10m elution. The highest amount of released residual monomer from all groups (except the control group) was TEGDMA, whereas this was HEMA for the control group. The amounts of residual monomers eluted from BAG30 were significantly higher than other groups (p<0.05). Conclusions: The release of the monomer increases in accordance with the increased BAG addition to the composite resins.Öğe Periostitis Ossificans Managed with Endodontic Treatment(Amer Soc Dentistry Child, 2015) Kucukyilmaz, Ebru; Sener, Yagmur; Tosun, Gul; Savas, SelcukPeriostitis ossificans (PO) is a specific type of chronic osteomyelitis. Radiographically, it is characterized by the presence of lamellae of newly formed periosteal bone outside the cortex, giving the characteristic appearance of onion skin. The most common cause of PO is periapical infection of the mandibular permanent first molar, which occurs primarily in children and young adults. Treatment in these cases is directed toward eliminating the source of the infection, either by extraction of the offending tooth or endodontic therapy. The purpose of this paper is to present a case of PO involving a permanent mandibular first molar managed with endodontic treatment.
