Yazar "Szulc, Piotr" seçeneğine göre listele
Listeleniyor 1 - 4 / 4
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Ethyl Methanesulfonate (EMS) Mutagen Toxicity-Induced DNA Damage, Cytosine Methylation Alteration, and iPBS-Retrotransposon Polymorphisms in Wheat (Triticum aestivum L.)(Mdpi, 2023) Turkoglu, Aras; Haliloglu, Kamil; Tosun, Metin; Bujak, Henryk; Eren, Baris; Demirel, Fatih; Szulc, PiotrThe use of mutagens in plant breeding is used to create new germplasm, increase agricultural yield, quality, and resistance to diseases and pests. Mutagens are physical or chemical factors that can alter the DNA or RNA structure of an organism, causing mutations above the expected level. One of the most common and potent chemical mutagens is EMS (ethyl-methane sulfonate), which produces point mutations in plants, but to a lesser degree can also cause the loss or deletion of a chromosomal region. This study used inter-primer binding site (iPBS) and coupled restriction enzyme digestion inter-primer binding site (CRED-iPBS) technique analysis to determine the effect of EMS mutagens on methylation rates in wheat genotypes at seedling growth stage. Treatments with five different EMS concentrations (0%; control, 0.1%, 0.2%, 0.3%, and 0.4%) at four different times (0; control, 3, 6, and 9 h) were used. Inter-primer binding site (iPBS) markers were employed to assess genomic instability and cytosine methylation in treated wheat. In seeds treated with EMS at different concentrations and times, the disappearance of regular bands and the formation of new bands due to the effects of the EMS mutagen revealed that genetic diversity exists. The CRED-iPBS analysis revealed that the 3 h + 0.1% EMS treatment produced the highest MspI polymorphism value (19.60%), while the 9 h + 0.1% EMS treatment produced the lowest value (10.90%). The mutagenic effects of EMS treatments had considerable polymorphism on a variety of impacts on the cytosine methylation and genomic instability of wheat. According to the current research, EMS mutagenesis may be a practical method for accelerating breeding programs to produce enough genetic diversity in wheat populations. Mutation-assisted breeding and the subsequent selection of desirable mutants using genetic markers may also be carried out in wheat utilizing an integrated strategy.Öğe Genotype-Trait (GT) Biplot Analysis for Yield and Quality Stability in Some Sweet Corn (Zea mays L. saccharata Sturt.) Genotypes(Mdpi, 2023) Stansluos, Atom Atanasio Ladu; Ozturk, Ali; Niedbala, Gniewko; Turkoglu, Aras; Haliloglu, Kamil; Szulc, Piotr; Omrani, AliA strong statistical method for investigating the correlations between traits, assessing genotypes based on numerous traits, and finding individuals who excel in particular traits is genotype-trait (GT) biplot analysis. The current study was applied to evaluate 11 sweet corn (Zea mays L. saccharata) genotypes and correlate them based on genotype-trait (GT) biplot analysis for two cropping seasons in Erzurum, Turkiye using the RCBD experimental design with three reputations. The results showed that the genotypes were significantly different for the majority of the examined variables according to the combined analysis of variance findings at 0.01 probability level. An ecological analysis was performed to evaluate sweet corn varieties and environmental conditions and interactions between them (genotype x environmental conditions). Our results showed that the summation of the first two and second main components was responsible for 73.51% of the combined cropping years of the sweet corn growth and development variance, demonstrating the biplot graph's optimum relative validity, which was obtained. In this study, the Khan F1 (G6) genotype was found to be the stablest genotype, and the Kompozit Seker (G7) genotype was the non-stable genotype, moreover based on the first cropping year, second cropping year, and the average mean of the two cropping years. As a conclusion, the Khan F1 (G6) genotype is the highest-yielding genotype, and the Kompozit Seker (G7) is the lowest. Based on the heat map dendrogram, the context of the differential extent of trait association of all genotypes into two clusters is indicated. The highest genetic distance was shown between the BATEM Tatli (G3) and Febris (G5) genotypes. Our results provide helpful information about the sweet corn genotypes and environments for future breeding programs.Öğe Identification of Novel QTLs Associated with Frost Tolerance in Winter Wheat (Triticum aestivum L.)(Mdpi, 2023) Bolouri, Parisa; Haliloglu, Kamil; Mohammadi, Seyyed Abolghasem; Turkoglu, Aras; Ilhan, Emre; Niedbala, Gniewko; Szulc, PiotrLow temperature (cold) and freezing stress is a major problem during winter wheat growth. Low temperature tolerance (LT) is an important agronomic trait in winter wheat and determines the plants' ability to cope with below-freezing temperatures; thus, the development of cold-tolerant cultivars has become a major goal of breeding in various regions of the world. In this study, we sought to identify quantitative trait loci (QTL) using molecular markers related to freezing tolerance in winter. Thirty-four polymorphic markers among 425 SSR markers were obtained for the population, including 180 inbred lines of F-12 generation wheat, derived from crosses (Norstar x Zagros) after testing with parents. LT50 is used as an effective selection criterion for identifying frost-tolerance genotypes. The progeny of individual F-12 plants were used to evaluate LT50. Several QTLs related to wheat yield, including heading time period, 1000-seed weight, and number of surviving plants after overwintering, were identified. Single-marker analysis illustrated that four SSR markers with a total of 25% phenotypic variance determination were linked to LT50. Related QTLs were located on chromosomes 4A, 2B, and 3B. Common QTLs identified in two cropping seasons based on agronomical traits were two QTLs for heading time period, one QTL for 1000-seed weight, and six QTLs for number of surviving plants after overwintering. The four markers identified linked to LT50 significantly affected both LT50 and yield-related traits simultaneously. This is the first report to identify a major-effect QTL related to frost tolerance on chromosome 4A by the marker XGWM160. It is possible that some QTLs are closely related to pleiotropic effects that control two or more traits simultaneously, and this feature can be used as a factor to select frost-resistant lines in plant breeding programs.Öğe Sodium Azide as a Chemical Mutagen in Wheat (Triticum aestivum L.): Patterns of the Genetic and Epigenetic Effects with iPBS and CRED-iPBS Techniques(Mdpi, 2023) Turkoglu, Aras; Haliloglu, Kamil; Tosun, Metin; Szulc, Piotr; Demirel, Fatih; Eren, Baris; Bujak, HenrykWheat, which is scientifically known as Triticum aestivum L., is a very nutritious grain that serves as a key component of the human diet. The use of mutation breeding as a tool for crop improvement is a reasonably rapid procedure, and it generates a variety that may be used in selective breeding programs as well as functional gene investigations. The present experiment was used to evaluate the potential application of a conventional chemical mutagenesis technique via sodium azide (NaN3) for the germination and seedling growth stage in wheat. Experiments with NaN3 mutagenesis were conducted using four different treatment periods (0, 1, 2, and 3 h) and five different concentrations (0, 0.5, 1, 1.5, and 2 mM). The genomic instability and cytosine methylation of wheat using its seeds were investigated after they were treated. In order to evaluate the genomic instability and cytosine methylation in wheat that had been treated, interprimer binding site (iPBS) markers were used. The mutagenic effects of NaN3 treatments had considerable polymorphism on a variety of impacts on the cytosine methylation and genomic instability of wheat plants. The results of the experiment showed considerable changes in the iPBS profiles produced by the administration of the same treatments at different dosages and at different times. Coupled restriction enzyme digestion interprimer binding site (CRED-iPBS) assays identified changes in gDNA cytosine methylation. The highest polymorphism value was obtained during 1 h + 2 mM NaN3, while the lowest (20.7%) was obtained during 1 h + 1.5 mM NaN3. Results showed that treatments with NaN3 had an effect on the level of cytosine methylation and the stability of the genomic template in wheat plants in the germination stage. Additionally, an integrated method can be used to for mutation-assisted breeding using a molecular marker system in wheat followed by the selection of desired mutants.