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Öğe Frequency of Adenovirus and Rotavirus and Their Seasonal Distribution in Children With Gastroenteritis(Aves, 2016) Tuzuner, Ugur; Gulcen, Begum Saran; Ozdemir, Mehmet; Feyzioglu, BahadirObjective: In our study, we aimed to investigate the prevalence of rotavirus and enteric adenovirus in stool samples sent to our laboratory for antigen detection of children between 0-18 years of age admitted to hospital with diarrhea, abdominal pain, vomiting and fever and diagnosed as gastroenteritis. We also analyzed their frequencies according to demographic parameters. Methods: Results of 5156 pediatric patients admitted to Nec-mettin Erbakan University Meram Faculty of Medicine Hospital and diagnosed as gastroenteritis between January 2013-December 2015 were investigated retrospectively. VIKIA (R) Rota-Adeno (bioMerieux, Marcy l'Etoile, France), a chromatographic immunoassay detecting both viruses simultaneously was used according to the manufacturer's recommendations in stool samples. Results: Viral antigens were detected in 884 (17.1%) of the total 5156 samples. 764 (14.8%) of the positive results were detected as rotavirus and 120 (2.3%) were detected as adenovirus. Of the patients with positive results, 412 (46.6%) were female and 472 (53.4%) were male. When results are considered according to age, 2-4 age group was found to have the most common positivity (n=372) as 42.1%. Seasonal distribution of acute gastroenteritis cases was analyzed and the number of cases due to rotavirus was found to be increased in winter and spring and enteric adenoviruses were detected all year round. Conclusions: Rotavirus is the most common reason of gastroenteritis in the newborn and children, which must be considered for patients with diarrhea especially in the first four years of life. Rapid diagnosis is important for prediction of clinical implications and treatment. As enteric adenovirus is an important reason of gastroenteritis in infancy and childhood, it is necessary to investigate adenovirus antigens as well. Conducting regional studies are important for contributing to epidemiological data.Öğe Prevalence and Concomitancy of Respiratory Viruses in Children with Acute Respiratory Tract Infections(Georg Thieme Verlag Kg, 2016) Tuzuner, Ugur; Akkaya, Oya; Ozdemir, Mehmet; Kurtoglu, Muhammet GuzelRespiratory tract infections (RTI) are among the most commonly seen infections in children across the world, with the highest rate of morbidity. Viruses are known to be the primary agent in these infections and mostly lead to upper RTIs. The most commonly seen viral agents of RTIs may be listed as rhinoviruses (HRV), influenza, parainfluenza (PIV), respiratory syncytial virus (RSV), adenovirus, and enteroviruses. In this study, prediagnosed children with acute RTI between January 2013 and May 2015 were included. Nasopharyngeal swab samples obtained from a total of 2,268 patients admitted to the hospital of Meram Medical School of Necmettin Erbakan University and Ministry of Health Konya Training and Research Hospital were investigated. The nasopharyngeal swab samples obtained in the hospital of Meram Medical School were investigated via Seeplex RV12 ACE Detection multiplex PCR (Seegene, South Korea) while the samples in Konya Training and Research Hospital were assessed using the system by CLART PneumoVir (Genomica, Spain). Of the 2,268 samples investigated in the study, 1,320 (58.2%) were detected for viruses. Positive number of viruses found in both kits were collected for use in a table, and the most common viruses rates were calculated according to these numbers. Among positive 1,221 samples, 27.9% were found to be RSV B, and this rate was, in turn, followed by HRV (18.8%) and RSV A. The most frequent concomitant of double viral agents was observed in RSV B and HRV as 14% followed by RSV A and HRV as 6.8%, and PIV 3 and HRV with 5.9%. While RSV B and RSV A were encountered most in winter and spring months, HRV was determined as an infectious agent in all seasons. Multiplex polymerase chain reaction is beneficial for physicians to diagnose such viruses at an early stage. By the early detection of respiratory viruses leading to seasonal epidemics, physicians' approach to patients will become easier, and unnecessary use of antibiotics will be prevented. Additionally, our study findings are intriguing in shedding light on studies related to the development and application of vaccines.Öğe Screening blood donors by nucleic acid amplification technology in Turkey(E-Century Publishing Corp, 2017) Ozdemir, Mehmet; Tuzuner, Ugur; Feyzioglu, Bahadir; Baykan, Mahmut; Baysal, BulentVolunteer blood donors are screened for hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) infection by various immunoassay methods in Turkey. The risk of enzyme immunoassay (EIA) negative and positive nucleic acid amplification technology (NAT) samples is not clearly understood yet. The purpose of this study is to screen for such donors in Turkey by a commercially available multiplex NAT test. All donors were screened by EIA and then NAT was performed on pools of six blood sera. When NAT reactive pools were determined they resolved to test the single donation samples. Single donor positive NAT sera were discriminated by polymerase chain reaction (PCR)-based diagnostic assay (COBAS AmpliScreen, Roche, USA). Incompatible sample results with EIA and NAT were searched with additional serologic and confirmatory tests. A total of 3000 donors were screened and detected seronegative, 9 HBV NAT cases (0.3%) and 1 HCV (0.03%) and 1 HIV NAT case (0.03%) were detected positively. Follow-up these donors were showed that the HCV yield case was a window period and all HBV NAT yield cases were occult carriers. The use of NAT will detect occult HBV and reduce window period in HCV. The yield rate, especially in occult HBV, was higher than that in non-endemic countries like Europian countries. Therefore, for routine donor screening by NAT will be provided safer blood transfusion in Turkey cost-effectively.Öğe The seroprevalence of HBV, HCV and HIV in blood donors(Elsevier Science Bv, 2016) Tuzuner, Ugur; Ozdemir, Mehmet; Feyzioglu, Bahadir; Baykan, Mahmut[Abstract Not Availabe]
