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    Effect of zinc sulfate supplementation on metallothionein levels in rat heart tissue with induced ischemia-reperfusion injury
    (Biointerface Research Applied Chemistry, 2018) Yazgan, Betul; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa Cihat; Sahna, Engin
    Metallothioneins are remarkable proteins with regard to their role in the regulation of intracellular zinc balance and mediation of the physiological effects of zinc, as well as their antioxidant effects. The objective of the present study is to examine how zinc supplementation impacts metallothionein levels in ischemia-reperfusion injury in rats. The study involved heart tissues obtained from 30 Wistar-Albino adult male rats already used in another project. Experimental animals were equally divided into three groups as follows: Group 1: Control (H-Cont); Group 2: Heart Ischemia/Reperfusion (H-I/R); and Group 3: Zinc supplemented Ischemia/Reperfusion (H/Zn-I/R). Cardiac ischemia-reperfusion procedures were carried out under general anesthesia. In the zinc-supplemented cardiac I/R group (Group 3), the animals were supplemented with 5 mg/kg/day intraperitoneal (i.p.) zinc sulfate per rat for 21 days. At the end of the procedures, all animals were decapitated and heart tissues were collected. The tissues were subjected to immunohistochemical staining procedures using rat metallothionein antibody. The stained preparations were photographed and cells stained with metallothionein were counted at a light microscope to calculate their percentages. The analyzed heart tissue samples of the groups did not have any significant difference in terms of their metallothionein levels. Results obtained from the study indicate that 21-day zinc supplementation does not have a critical effect on metallothionein synthesis in cardiac ischemia-reperfusion. This result may be attributed to the dose of zinc, length of supplementation and/or duration of ischemia-reperfusion.
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    Effects of zinc supplementation on metallothionein levels in ischemic renal tissue
    (Taylor & Francis Ltd, 2020) Yazgan, Betul; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa Cihat
    We investigated how zinc (Zn) supplementation affects metallothionein levels in the cortex and medulla of ischemic renal tissue of rats. We used adult male rats divided into four groups: group 1, untreated control; group 2, sham-operated; group 3, ischemia-reperfusion; group 4, ischemia-reperfusion + 5 g/kg Zn. Renal tissue was analyzed using immunostaining of rat metallothionein. Cells stained with metallothionein were counted and their percentage was calculated. We found that the Zn supplemented ischemia and reperfusion group exhibited a greater percentage of cells stained strongly for metallothionein in the renal cortex than all other groups. In the renal medulla, percentages of weak staining for metallothionein in the control and ischemia and reperfusion groups were greater than those in the sham and Zn-supplemented ischemia/reperfusion groups. Our findings indicate that the main effect of Zn in the renal tissue occurs in the cortex, while metallothionein synthesis in the renal medulla is unaffected.
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    Zinc is a strong stimulant of metallothionein synthesis in the ischaemic testis tissue
    (Wiley, 2021) Yazgan, Betul; Baltaci, Abdulkerim Kasim; Mogulkoc, Rasim; Avunduk, Mustafa Cihat
    This study was performed to determine the effect of zinc supplementation effects on metallothionein levels in testis ischaemia-reperfusion of rats. The experimental groups were designed as Control, Sham, Ischaemia-Reperfusion (I/R) and I/R + Zinc supplemented. Zinc supplemented as 5 mg/kg day for 3 weeks. Testis tissues were analysed for metallothionein by immunohistochemical staining procedures. Group comparison showed that the zinc-supplemented ischaemia-reperfusion group had a significantly higher level of cells strongly stained with metallothionein than all other groups. A general evaluation of the results suggests that zinc supplementation is a strong stimulant of metallothionein synthesis in the ischaemic testis tissue.