The effect of vanillic acid on ligature-induced periodontal disease in Wistar rats

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dc.contributor.authorKaratas, Ozkan
dc.contributor.authorYuce, Hatice Balci
dc.contributor.authorTaskan, Mehmet Murat
dc.contributor.authorGevrek, Fikret
dc.contributor.authorYarkac, Fatma Ucan
dc.contributor.authorKeskin, Ash
dc.contributor.authorKaratas, Sukruye Firuze Ocak
dc.date.accessioned2024-02-23T14:02:08Z
dc.date.available2024-02-23T14:02:08Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractObjective: Vanillic acid, also known as 4-hydroxy-3-methoxy benzoic acid has a potent effect on bone metabolism. The purpose of the present study was to specify the effects of vanillic acid (VA) on preventing inflammation and bone destruction in experimental periodontitis as inflammatory bone disease. To evaluate the effects of VA, osteoblast, osteoclast and inflammatory cell counts, iNOS, CD68, MMP-1, and TIMP-1 levels were determined. Methods: 32 female Wistar rats were divided into four experimental groups as; Group 1: healthy control (C, n = 8), group 2: Periodontitis (P, n = 8), group 3: periodontitis and 50 mg/kg VA administered group (P + VA 50, n = 8) and group 4: periodontitis and 100 mg/kg VA delivered group (P + VA-100, n = 8). Ligature-induced experimental periodontitis was carried out at mandibular first molar teeth of the right quadrant by placing submarginal 4-0 silk ligatures. VA was administered by oral gavage for 14 days beginning from the first day. Rats were euthanized on the 15th day. Morphological changes in alveolar bone were evaluated via a stereomicroscope. Mandibles were subjected to histological procedures. Osteoblasts, tartrate-resistant acid phosphatase synthesizing osteoclasts and inflammatory cells were counted. Inducible nitric oxide synthase (iNOS), cluster of differentiation (CD)-68, Matrix metalloproteinase (MMP)-1, tissue inhibitor of MMP-1, runt-related x factor-2 (RUNX2), and cyclooxygenase (COX)-2 expressions were determined by immunohistochemistry. Results: The rats in the periodontitis group had the highest alveolar bone loss compared to the other groups. Both doses of VA significantly decreased alveolar bone loss but not the control levels. TRAP-positive osteoclast and inflammatory cell counts were also highest in the P group, and both 50 and 100 mg/kg VA reduced these counts. Control rats had the lowest osteoclast and inflammatory cell counts compared to the other groups. Similar to osteoclast counts, MMP-1, iNOS, CD68, and COX-2 expressions were the highest in the P group compared to the other groups. Both doses of VA significantly decreased these levels. Osteoblast cells were higher in the VA groups compared to the control and periodontitis groups. RUNX2 levels were lower in the periodontitis group compared to the control group. A slight increase was also observed in VA groups. However, the difference in the TIMP-1 levels was significant only between P and VA100 groups. Conclusion: VA administration successfully ameliorated periodontitis symptoms by decreasing alveolar bone and collagen destruction, periodontal inflammation, and increasing osteoblastic activity.en_US
dc.identifier.doi10.1016/j.archoralbio.2019.05.010
dc.identifier.endpage7en_US
dc.identifier.issn0003-9969
dc.identifier.issn1879-1506
dc.identifier.pmid31103800en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.archoralbio.2019.05.010
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11603
dc.identifier.volume103en_US
dc.identifier.wosWOS:000472590300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofArchives Of Oral Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlveolar Boneen_US
dc.subjectAntioxidantsen_US
dc.subjectCd68en_US
dc.subjectExperimental Periodontitisen_US
dc.subjectInosen_US
dc.subjectVanillic Aciden_US
dc.titleThe effect of vanillic acid on ligature-induced periodontal disease in Wistar ratsen_US
dc.typeArticleen_US

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