Development of transgenic tobacco lines with pyramided insect resistant genes

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dc.contributor.authorBakhsh, Allah
dc.contributor.authorDinc, Tolga
dc.contributor.authorHussain, Tahira
dc.contributor.authorDemirel, Ufuk
dc.contributor.authorAasim, Muhammad
dc.contributor.authorCaliskan, Mehmet Emin
dc.date.accessioned2024-02-23T14:35:27Z
dc.date.available2024-02-23T14:35:27Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractInsect pests are among the major constraints rendering drastic decreases in crop yield. 'I he expression of stacked insecticidal genes in crops can lead to resistance durability and can delay the development of resistance in target insect pests. The present study was designed to introduce an insect resistance trait in locally cultivated Turkish tobacco cultivars (Basma and Nail) with pyramided insecticidal genes. Agrobacterium strain LBA4404 harboring plasmid pKGH4 with cry1Ac and cry2A genes under the control of 35S promoter was used to infect leaf discs of both cultivars; plasmid also contained uidA within the T-DNA region for earlier screening of putative transformants. The overall transformation efficiency was calculated as 30.7% and 18.8% in Basma and Nail, respectively. PCR results confirmed the integration of cry1Ac, cry2A, uidA, and nptII genes in 40 plants of Basma and 16 plants of Nail. ELISA results showed variation in expression of cry1Ac protein among transgenic plants varying from 0.017 to 0.607 mu g/g of fresh tissue. Bioassay results with potato tuber moth (Phthorimea operculella Zeller) showed significant mortality of the targeted pest on primary transfonnants. Furthermore, T1 transgenic progeny exhibited the inheritance of T-DNA in Mendelian as well as non-Mendelian fashion. The results revealed that lines can serve as a source of germplasm in tobacco breeding programs.en_US
dc.description.sponsorshipNigde Omer Halisdemir University Research Project Unit [FEB 2014/10-BAGEP]en_US
dc.description.sponsorshipThe present research project was supported by a grant from Nigde Omer Halisdemir University Research Project Unit (Project No. FEB 2014/10-BAGEP).en_US
dc.identifier.doi10.3906/biy-1710-71
dc.identifier.endpage186en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue2en_US
dc.identifier.pmid30814879en_US
dc.identifier.scopus2-s2.0-85046024111en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage174en_US
dc.identifier.urihttps://doi.org/10.3906/biy-1710-71
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16037
dc.identifier.volume42en_US
dc.identifier.wosWOS:000433481900008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTobaccoen_US
dc.subjectGenetic Transformationen_US
dc.subjectProtein Expressionen_US
dc.subjectInsect Resistanceen_US
dc.titleDevelopment of transgenic tobacco lines with pyramided insect resistant genesen_US
dc.typeArticleen_US

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