Lysates from the probiotic bacterium Streptococcus thermophilus enhances the survival of T cells and triggers programmed cell death in neuroblastoma cells

Basit Öğe Kaydı
dc.contributor.authorAltves, Safaa
dc.contributor.authorGuclu, Ebru
dc.contributor.authorAyan, Ilknur Cinar
dc.contributor.authorBilecen, Kivanc
dc.contributor.authorVural, Hasibe
dc.date.accessioned2024-02-23T13:59:47Z
dc.date.available2024-02-23T13:59:47Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractNeuroblastoma is the most common brain solid tumor in infancy. Despite the availability of numerous approaches like immunotherapy, surgery, chemotherapy, and radiotherapy, neuroblastoma frequently develops resistance and recurs. Immunotherapy is one of the most promising approaches and PD-L1 antibody blocking is the phenomena used to inhibit PD-1 receptors to increase and improve cytotoxic T cells toward cancer. Numerous studies underlined the critical role of probiotics on immune system development and modulation in addition to possible role in inducing apoptosis in cancer cells. In this study, a Streptococcus thermophilus strain, isolated from a local yogurt, was used as it is considered a potential probiotic due to its tolerance lower pH, bile acid, antibiotic suitability, and blood hemolysis. Our results showed that S. thermophilus lysates played as an immune checkpoint modulator at 25 mu g/ml dose boosting PD-L1 transcripts and protein levels in SH-SY5Y neuroblastoma cell line. Interestingly, co-culture between SH-SY5Y and Jurkat T cells in the presence of blocking PD-L1 antibodies increased Jurkat T-cell viability compering to control without lysate. On the other hand, annexin-V/7-AAD, qPCR and western blot results showed that S. thermophilus lysates at 200 and 400 mu g/ml decreased SH-SY5Y cell viability and increased apoptotic marker genes transcription and caspase-3 and caspase-9 protein expression.en_US
dc.identifier.doi10.1007/s12032-023-02186-4
dc.identifier.issn1357-0560
dc.identifier.issn1559-131X
dc.identifier.issue11en_US
dc.identifier.pmid37787808en_US
dc.identifier.scopus2-s2.0-85173066964en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s12032-023-02186-4
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11325
dc.identifier.volume40en_US
dc.identifier.wosWOS:001079145900002en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofMedical Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectImmunotherapyen_US
dc.subjectStreptococcus Thermophilusen_US
dc.subjectProbioticen_US
dc.subjectPd-L1en_US
dc.titleLysates from the probiotic bacterium Streptococcus thermophilus enhances the survival of T cells and triggers programmed cell death in neuroblastoma cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu