Investigation of anticancer mechanism of oleuropein via cell cycle and apoptotic pathways in SH-SY5Y neuroblastoma cells

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dc.contributor.authorSecme, Mucahit
dc.contributor.authorEroglu, Canan
dc.contributor.authorDodurga, Yavuz
dc.contributor.authorBagci, Gulseren
dc.date.accessioned2024-02-23T14:02:58Z
dc.date.available2024-02-23T14:02:58Z
dc.date.issued2016
dc.departmentNEÜen_US
dc.description.abstractNeuroblastoma is one of the most common types of pediatric tumors that can spread quickly in neuronal tissues. Oleuropein which is active compound of olive leaves, belongs to polyphenols group and has antioxidant, antimicrobial, anti-inflammatory, anti-hypertensive and anti-carcinogenic effects. The aim of the study is to determine the therapeutic effects of oleuropein on cell proliferation, invasion, colony formation, cell cycle and apoptotic mechanisms in SH-SY5Y neuroblastoma cell line under in vitro conditions. The effect of oleuropein on cell viability was determined by KIT method. 84 cell cycle control and 84 apoptosis related genes were evaluated by RT-PCR. Effects of oleuropein on apoptosis were researched by TUNEL assay. Protein expressions were determined by western blot analysis. Effects of oleuropein on cell invasion, colony formation and migration were detected by matrigel-chamber, colony formation assay and wound-healing assay, respectively. IC50 value of oleuropein in SH-SY5Y cells was detected as 350 IN at 48th hours. It is determined that oleuropein causes cell cycle arrest by down-regulating of CylinD1,CylinD2,CyclinD3,CDK4,CDK6 and up-regulating of p53 and CDKN2A, CDKN2B, CDKN1A gene expressions. Oleuropein also induces apoptosis by inhibiting of Bcl-2 and activating of Bax,caspase-9 and caspase-3 gene expressions. Apoptotic cell ratio was found 36.4 +/- 3.27% in oleuropein dose group. Oleuropein decreased invasion in SH-SY5Y cells and suppressed colony numbers in ratio of 53.6 +/- 4.71%.Our results demonstrated that oleuropein can be a therapeutic agent in the treatment of neuroblastoma. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipInstructor Training Program of Pamukkale University; Pamukkale Universityen_US
dc.description.sponsorshipThis study was produced from the MSc thesis of Mucahit Secme and supported by Instructor Training Program of Pamukkale University.en_US
dc.identifier.doi10.1016/j.gene.2016.03.038
dc.identifier.endpage99en_US
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.issue1en_US
dc.identifier.pmid27032461en_US
dc.identifier.scopus2-s2.0-84963699422en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage93en_US
dc.identifier.urihttps://doi.org/10.1016/j.gene.2016.03.038
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11921
dc.identifier.volume585en_US
dc.identifier.wosWOS:000375519400014en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofGeneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNeuroblastomaen_US
dc.subjectOleuropeinen_US
dc.subjectCell Cycleen_US
dc.subjectApoptosisen_US
dc.titleInvestigation of anticancer mechanism of oleuropein via cell cycle and apoptotic pathways in SH-SY5Y neuroblastoma cellsen_US
dc.typeArticleen_US

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