Effect of gibberellic acid on in vitro flowering from stem node explant of Anubias barteri var. Nana
Küçük Resim Yok
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Research Journal Biotechnology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Anubias barteri is a commercially important aquatic ornamental plant that has low multiplication rate and in vitro regeneration difficulties. Therefore, lack of scientific information is available. To increase the knowledge and function of plant growth regulators on Anubias regeneration, stem nodes explants were cultured in half and full-strength Murashige and Skoog media containing Gibberellic acid (GA(3)), Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA). The used plant growth regulators significantly (p <= 0.05) affected number of axillary shoots induction, shoot length, flower induction and number of flowers per explant. The growth pattern of in vitro regenerated axillary shoots was indicated from the shoot base of the stem node. Maximum number of axillary shoots (4.25 +/- 0.38), shoots length (2.63 +/- 0.21) and flowers (4.0) per explants were achieved on full strength MS medium supplemented with 1.00 mg L-1 GA(3), 0.10 mg L-1 BAP, 0.10 mg L-1 NAA and 2 g L-1 activated charcoal. The MS medium without growth regulators did not induce flower that showed the necessity of growth regulators for in vitro flowering. The root induction was statistically similar on half and full-strength MS medium containing different combination of applied growth regulators. The maximum root induction (91.66%) was noted on MS medium containing 0.25 mg L-1 GA(3), 0.10 mg L-1 BAP and 0.10 mg L-1 NAA. All regenerated plantlets were successfully acclimatized in tap water filled aquarium. The present results showed importance of plant growth regulators that control growth, morphogenetic processes and micropropagation of Anubias plant.
Açıklama
Anahtar Kelimeler
Axillary Shoots, Regeneration, Flowering, Proliferation, Pgrs, Stem Nodes
Kaynak
Research Journal Of Biotechnology
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
14
Sayı
9
