Bioactive Compounds of Rheum ribes L. and its Anticancerogenic Effect via Induction of Apoptosis and miR-200 Family Expression in Human Colorectal Cancer Cells

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dc.contributor.authorAyan, Ilknur Cnar
dc.contributor.authorCetinkaya, Sumeyra
dc.contributor.authorDursun, Hatice Gul
dc.contributor.authorSuntar, Ipek
dc.date.accessioned2024-02-23T14:17:14Z
dc.date.available2024-02-23T14:17:14Z
dc.date.issued2021
dc.departmentNEÜen_US
dc.description.abstractRheum ribes L. is a widespread plant species in the eastern part of our country and is consumed as a vegateble by the folk people. In our study, we investigated whether R. ribeshas anti-cancerogenic effect on colorectal cancer cell lines. Six different extracts were obtained from R. ribes. Total phenolic, flavonoid contents, antioxidant activities, and cytotoxic effects of all extracts on colorectal cancer cells were determined. Differences in expression levels of the miR-200 family and target genes were assessed by real-time qPCR in cells. Antiproliferative effect was determined by TUNEL, Annexin V, cell cycle, invasion analysis; and levels of BCL-2, ZEB1, GATA4 and FAS/CD95 proteins were determined by ELISA. According to the results of miRNA expression analysis, the only dose group with a significant increase (p < 0,05) in the expression level of all of miRNAs in both cell lines was RM (Root methanol) extract alone. Our results showed that, RM caused a significant increase in the expressions of miR-200a/b/c and miR-141, and it suppressed BCL-2, ZEB1, GATA4 expressions, with this way. Thus, it has been suggested that the treatment of RM alone, or combined treatment of RM with 5-FU result in a better response than cancer cells treated with 5-FU alone.en_US
dc.description.sponsorshipscientific research projects foundation of Necmettin Erbakan university [161418001]en_US
dc.description.sponsorshipThis work was supported by scientific research projects foundation of Necmettin Erbakan university (Grant number: 161418001).en_US
dc.identifier.doi10.1080/01635581.2020.1792947
dc.identifier.endpage1243en_US
dc.identifier.issn0163-5581
dc.identifier.issn1532-7914
dc.identifier.issue7en_US
dc.identifier.pmid32691634en_US
dc.identifier.scopus2-s2.0-85088293861en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1228en_US
dc.identifier.urihttps://doi.org/10.1080/01635581.2020.1792947
dc.identifier.urihttps://hdl.handle.net/20.500.12452/13009
dc.identifier.volume73en_US
dc.identifier.wosWOS:000550551300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherRoutledge Journals, Taylor & Francis Ltden_US
dc.relation.ispartofNutrition And Cancer-An International Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[Keyword Not Available]en_US
dc.titleBioactive Compounds of Rheum ribes L. and its Anticancerogenic Effect via Induction of Apoptosis and miR-200 Family Expression in Human Colorectal Cancer Cellsen_US
dc.typeArticleen_US

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