Alpha-Amanitin Poisoning, Nephrotoxicity and Oxidative Stress: An Experimental Mouse Model

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dc.contributor.authorErgin, Mehmet
dc.contributor.authorDundar, Zerrin Defne
dc.contributor.authorKilinc, Ibrahim
dc.contributor.authorColak, Tamer
dc.contributor.authorOltulu, Pembe
dc.contributor.authorGirisgin, Abdullah Sadik
dc.date.accessioned2024-02-23T14:44:44Z
dc.date.available2024-02-23T14:44:44Z
dc.date.issued2015
dc.departmentNEÜen_US
dc.description.abstractBackground: Alpha-amanitin (alpha-AMA) plays a major role in Amanita phalloides poisoning, showing toxic effects on multi-organs, particularly on the liver and kidneys. Studies have shown a relationship between alpha-AMA-related injuries and reactive oxygen species. Objectives: We aimed to investigate whether there is renal injury and its relationship with oxidative stress after intraperitoneal injection of alpha-AMA in mice experimental poisoning models. Materials and Methods: There were 37 male BALB/c laboratory mice treated with alpha-AMA, according to the study groups: control group (n = 7); low dose (0.2 mg/kg) (n = 10); moderate dose (0.6 mg/kg) (n = 10), and high dose (1 mg/kg) (n = 10). The sample size was detected according to the ethical committee's decision as well as similar studies in the literature. After a 48-hour follow-up period, all the subjects were sacrificed for pathological and biochemical assays. The study was held in Turkey. Results: alpha-AMA poisoning in mice results in inflammatory changes and necrosis in renal structures. There were statistically significant differences between the study groups regarding measured levels of catalase, superoxide dismutase, glutathione peroxidase, total antioxidant status (TAS), total oxidant status (TOS) and malonyl dialdehyde in renal homogenates of mice (P < 0.001, P < 0.001, P < 0.001, P < 0.001, P < 0.001, and P = 0.001, respectively). The TOS and TAS measurements helped to eliminate cumbersome analysis of diverse oxidant and antioxidant molecules. The TOS levels in renal homogenate of mice were significantly higher in all the intoxication groups compared to the control group (5.73, 7.02, 7.77, and 9.65 mmol trolox eq/g protein and P = 0.002, P = 0.001, and P = 0.001, respectively). The TAS levels in moderate and high-dose groups were significantly lower than all the other groups treated with alpha-AMA (0.130, 0.152, 0.065, and 0.087 mmol trolox eq/g protein and P = 0.031, P = 0.001, and P = 0.001, respectively). Conclusions: Our results indicated that alpha-AMA poisoning in mice led to inflammatory changes and necrosis in renal structures. Biochemical analysis showed a shift in the oxidative/anti-oxidative balance towards the oxidative status.en_US
dc.description.sponsorshipNecmettin Erbakan university, department of scientific research project [141218002]en_US
dc.description.sponsorshipThe study was supported by Necmettin Erbakan university, department of scientific research projects; research project No. 141218002.en_US
dc.identifier.doi10.5812/ircmj.28068
dc.identifier.issn2074-1804
dc.identifier.issn2074-1812
dc.identifier.issue8en_US
dc.identifier.pmid26430527en_US
dc.identifier.scopus2-s2.0-84939612728en_US
dc.identifier.urihttps://doi.org/10.5812/ircmj.28068
dc.identifier.urihttps://hdl.handle.net/20.500.12452/17093
dc.identifier.volume17en_US
dc.identifier.wosWOS:000362367900011en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKowsar Publen_US
dc.relation.ispartofIranian Red Crescent Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlpha-Amanitinen_US
dc.subjectOxidative Stressen_US
dc.subjectMycotoxinsen_US
dc.titleAlpha-Amanitin Poisoning, Nephrotoxicity and Oxidative Stress: An Experimental Mouse Modelen_US
dc.typeArticleen_US

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