Inhibitory effect of AK-7 mediates by apoptosis, increases DNA fragmentation and caspase-3 activity in human glioblastoma multiforme cells

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dc.contributor.authorGuclu, Ebru
dc.contributor.authorAyan, Ilknur Cinar
dc.contributor.authorVural, Hasibe
dc.date.accessioned2024-02-23T14:34:51Z
dc.date.available2024-02-23T14:34:51Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractSirtuins (SIRTs) which are nicotinamide adenine dinucleotide (NAD+) dependent class III histondeacetylases have a controversial role in cancer. In this study, the effect of pharmacological inhibition of AK-7, a SIRT2 inhibitor, was investigated in U87 glioblastoma multiforme cells. The cytotoxic effect of AK-7 was evaluated by XTT analysis. After AK-7 treatment, colony forming capacity of cells was determined and apoptosis was evaluated. The expression levels of apoptosis-related genes were determined by qRT-PCR. According to the results, AK-7 inhibited cell proliferation in a dose-and time-dependent manner. After AK-7 treatment, the colony forming capacity of U87 cells was suppressed. And, AK-7 increased apoptosis rate, DNA fragmentation, and caspase-3 activity. According to qRT-PCR, a significant increase was observed in expression levels of apoptosis-related genes. This study revealed that AK-7 inhibits cell proliferation and induces apoptosis in glioblastoma multiforme cells and SIRT2 inhibition can be evaluated as a therapeutic approach in glioblastoma multiforme.en_US
dc.identifier.doi10.3329/bjp.v17i2.59809
dc.identifier.endpage50en_US
dc.identifier.issn1991-007X
dc.identifier.issn1991-0088
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85132132729en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage42en_US
dc.identifier.urihttps://doi.org/10.3329/bjp.v17i2.59809
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15768
dc.identifier.volume17en_US
dc.identifier.wosWOS:000809423000003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBangladesh Pharmacological Socen_US
dc.relation.ispartofBangladesh Journal Of Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[Keyword Not Available]en_US
dc.titleInhibitory effect of AK-7 mediates by apoptosis, increases DNA fragmentation and caspase-3 activity in human glioblastoma multiforme cellsen_US
dc.typeArticleen_US

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