SARS-CoV-2 Genome Analysis of Pediatric Patients in Konya Region, Turkey

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dc.contributor.authorTasbent, Fatma E.
dc.contributor.authorOzdemir, Mehmet
dc.contributor.authorAkcan, Ozge M.
dc.contributor.authorKurt, Esma K.
dc.date.accessioned2024-02-23T14:16:59Z
dc.date.available2024-02-23T14:16:59Z
dc.date.issued2021
dc.departmentNEÜen_US
dc.description.abstractObjective Genome sequencing is useful for following the change in mutation and variants in viral agent during pandemics. In this study, we performed next-generation sequencing of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) complete genomes on pediatric patients. Methods Six pediatric patients aged 0 to 18 years who were positive for SARS-CoV-2 by reverse transcription polymerase chain reaction were included in this study. SARS-CoV-2 genome sequencing was performed using Oxford Nanopore Technologies MinION, following the ARTIC Network protocols. Sequencing data were obtained using the FASTQ program and quality assessment was evaluated. The sequence information of all samples was uploaded to the Global Initiative on Sharing All Influenza Data (GISAID) database. Genome, variant, clade, and phylogenetic tree analyses were performed with bioinformatic analysis. Results Two of these six samples were at 20A, two were at 20B, and two were at 19A in the nextstrain clade. According to Pango lineages, B.1.36, B.1.218, B.1, and B.1.260 lineages were detected. A total of 84 mutations were observed in all samples. None of the variants were classified as variants of concern (VOC) nor variants of interest (VOI) according to the Pango database. Conclusion This study is the first comprehensively sequence analysis registered in the GISAID database reported from the Konya region in Turkey. Similar studies will be informative to track changes in the virus genome, obtain epidemiological data, guide studies on diagnosis and treatment, and evaluate vaccine efficacy.en_US
dc.identifier.doi10.1055/s-0041-1736561
dc.identifier.endpage302en_US
dc.identifier.issn1305-7707
dc.identifier.issn1305-7693
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85118291264en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage296en_US
dc.identifier.urihttps://doi.org/10.1055/s-0041-1736561
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12877
dc.identifier.volume16en_US
dc.identifier.wosWOS:000709520100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofJournal Of Pediatric Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCovid-19en_US
dc.subjectSars Cov-2en_US
dc.subjectVariationen_US
dc.subjectGenome Sequencingen_US
dc.subjectCladeen_US
dc.titleSARS-CoV-2 Genome Analysis of Pediatric Patients in Konya Region, Turkeyen_US
dc.typeArticleen_US

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