Extended-spectrum of KRAS and NRAS mutations in lung cancer tissue specimens obtained with bronchoscopy

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dc.contributor.authorBasdemirci, Muserref
dc.contributor.authorZamani, Adil
dc.contributor.authorZamani, Ayse G.
dc.contributor.authorFindik, Siddika
dc.contributor.authorYildirim, Mahmut S.
dc.date.accessioned2024-02-23T14:37:47Z
dc.date.available2024-02-23T14:37:47Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractBackground: Mutations in the RAS genes, HRAS, KRAS, and NRAS, are the most common modifications in many types of human tumors and are found in approximately 30% of all human cancers. These mutations are usually found in codons 12, 13, or 61. Methods: The aim of this study is to evaluate mutations in codons 59, 117, and 146 of KRAS and NRAS genes in addition to codons 12,13, and 61 of KRAS gene in lung cancer tissue specimens obtained with bronchoscopy. KRAS and NRAS mutation analyses with pyrosequencing were performed on DNA isolated from formalin-fixed paraffinembedded (FFPE) tissue samples of 64 patients histopathologically diagnosed as lung cancer after bronchoscopic biopsy. Results: In all, 20 patients (31.2%) had mutations in KRAS gene (8/27 squamous cell carcinoma, 8/11 adenocarcinoma, 3/16 small cell carcinoma, and 1/1 pleomorphic carcinoma). The most common mutation in codon 12 was in c.35G>T (G12V). When the mutation rate of adenocarcinoma (72.7%) and squamous cell carcinoma (22.9%) patients was compared with each other, a statistically significant difference was observed (P = 0.008). There were no mutations in codons 59, 117, or 146 of KRAS and NRAS genes in patients with lung cancer. Conclusion: In this study, we firstly examined mutations in codons 59, 117, and 146 of KRAS and NRAS genes in addition to codons 12, 13, and 61 of KRAS gene in Turkish lung cancer patients both in non-small cell lung cancer and small cell lung cancer. Although no mutation was detected in codons 59, 117, and 146 of KRAS and NRAS genes, the frequency of KRAS gene mutation was higher than the rate of mutation in both Asian and Western countries, and multicenter studies including more cases should be performed to further explore our results.en_US
dc.description.sponsorshipScientific Research Project of Necmettin Erbakan University [151518011]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Project of Necmettin Erbakan University (grant number 151518011).en_US
dc.identifier.doi10.4103/ijc.IJC_766_19
dc.identifier.endpage243en_US
dc.identifier.issn0019-509X
dc.identifier.issn1998-4774
dc.identifier.issue2en_US
dc.identifier.pmid34380837en_US
dc.identifier.scopus2-s2.0-85135768856en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage236en_US
dc.identifier.urihttps://doi.org/10.4103/ijc.IJC_766_19
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16240
dc.identifier.volume59en_US
dc.identifier.wosWOS:000920519900012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofIndian Journal Of Canceren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBronchoscopyen_US
dc.subjectKrasen_US
dc.subjectLung Canceren_US
dc.subjectMutationen_US
dc.subjectNrasen_US
dc.titleExtended-spectrum of KRAS and NRAS mutations in lung cancer tissue specimens obtained with bronchoscopyen_US
dc.typeArticleen_US

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