Effect of methylprednisolone loaded poly lactic-co-glycolic acid (PLGA) bioabsorbable nanofibers on tendon healing and adhesion formation

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dc.contributor.authorZuhour, Moath
dc.contributor.authorGunes, Cansu
dc.contributor.authorFindik, Siddika
dc.contributor.authorDundar, Mehmet Akif
dc.contributor.authorGok, Orhan
dc.contributor.authorAltuntas, Zeynep
dc.date.accessioned2024-02-23T14:12:45Z
dc.date.available2024-02-23T14:12:45Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractBackground: Nanomaterials have been widely used in many fields such as vaccination and drug delivery. Beside its behavior as a degradable physical barrier, it can provide a controlled drug release. Tendon healing is a process full of complications, one of which is adhesion caused by excessive fibrosis.Aim: In this study, we aimed to prevent adhesion formation by using methylprednisolone (MP) loaded Poly lactic-co-glycolic acid (PLGA) mats. We used PLGA mats as both, biodegradable physical barrier to reduce the contact between surrounding tissues and healing tendon and as a drug delivery vehicle to release the antifibrotic MP with a controlled pattern.Methods: MP-loaded PLGA nanofiber mats were produced using electrospinning technique under optimized pa-rameters. To find the optimal MP dose, the drug was loaded in 15%, 25% and 35% concentrations. In vitro analysis included FT-IR, antibacterial, water absorption, biodegradability and drug release behavior tests. 70 rats' tendons were used for this study. After scarification of the rats, tendons were analyzed in terms of macroscopic, histopathological and biomechanical evaluation.Results: The neat PLGA and 15%, 25%, and 35% MP-loaded nanofibers lost 47%, 83%, 88%, and 97% of their initial weights at the end of the 8 weeks' degradation process. Within the first 24 h, mats including higher drug concentrations showed more initial release burst effect than samples loaded with lower concentrations. Cumu-lative drug release at 24 h was 29.5%, 27.6%, and 24.7% for PLGA nanofiber samples containing 15%, 25%, 35% MP, respectively. Macroscopically, When the groups were compared, no statistically significant difference was found between group 1 (no surgical intervention) and group 5 (25% MP/PLGA) in terms of length, characteristics and degree of the adhesion. Comparing to other groups statistically significant atrophic effect was found in group 6 (35% MP/PLGA).Conclusion: 25% MP-loaded PLGA reduces the formation of adhesions macroscopically comparable to tendons that didn't receive any surgical intervention. Microscopically, it provides better tendon healing compared to tendons that received only surgical repair or surgery + neat PLGA. Methylprednisolone did not only add an antibacterial effect to PLGA but also increased the hydrophilic property and degradation rate of PLGA. Increased steroid concentration also leads to atrophy at the healing tendons which can be prevented by modifying the PLGA design.en_US
dc.identifier.doi10.1016/j.jddst.2023.104988
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.scopus2-s2.0-85173277126en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2023.104988
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12160
dc.identifier.volume89en_US
dc.identifier.wosWOS:001090967200001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Drug Delivery Science And Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdhesionen_US
dc.subjectCorticosteroidsen_US
dc.subjectFibrosisen_US
dc.subjectMethylprednisoloneen_US
dc.subjectPlgaen_US
dc.subjectTendonen_US
dc.titleEffect of methylprednisolone loaded poly lactic-co-glycolic acid (PLGA) bioabsorbable nanofibers on tendon healing and adhesion formationen_US
dc.typeArticleen_US

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